NEB 5-alpha Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemNEB 5-alpha Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $153.00 Inquire
Catalog NumberC2987I
Quantity6 x 0.2 ml
GenotypefhuA2Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
ApplicationsDNA Manipulation
SpeciesE. coli
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
1- 3 x 10⁹ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Comparative analysis reveals molecular adaptation of mammalian HCA2 to microbial metabolitesiScienceJune 19, 2026Franziska Bischof, Petra Krumbholz, Gunnar Kleinau, Patrick Scheerer, Claudia Stäubertstrains,Bacterial and virus strains,Bacterial and virus strains ,, NEB 5-alpha competent E.coli,NEB,C2987 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(2) Phage-mediated lysis increases growth rate of surviving bacterial cellsiScienceJune 19, 2026Emanuele Fara, Benjamin Raach, Alessio Cavallaro, Justus Fink, Divvya Ramesh, Yongzhao Guo, Victoria Orphan, Alex R. Hall, Gabriele Micali, Martin Ackermann, et al.numbers PV021963 and PV936258, respectively. Plasmids were extracted from E. coli competent cells (NEB #C2987H) using the QIAprep Spin Miniprep Kit (Qiagen #27104) and transformed into the WT and TS lysogenic strains.

(3) Distinct 2-phenylimidazo[1,2-a]pyridine derivatives that inhibit breast cancer cell proliferation identified as AHR ligandsiScienceJune 19, 2026Katrin Koellisch, Christine Blattner, Stefano Motta, Janine Wesslowski, Melanie Rothley, Simone Büchel, Savannah Sirounian, Ilenia Segatto, Hanna T. Weber, Julia Müller, et al.strain,Bacterial strain ,, NEB® 5-alpha Competent E. coli (High Efficiency),New England Biolabs,Cat# C2987H ,, Chemicals and recombinant proteins,Chemicals and recombinant proteins,Chemicals and recombinant

(4) Metabolic engineering enables Escherichia coli to grow on 1,3-propanediolSynthetic and Systems BiotechnologyJune 1, 2026Nga Yu Poon, Anthony J. Sinskey, Kang Zhouconstruction, sequencing and strain engineering Newly constructed plasmids were propagated in Dh5α (NEB C2987H) overnight and extracted in accordance with the protocol provided in the QIAprep Spin Miniprep Kit (Qiagen).

(5) International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equitySci AdvMay 29, 2026Severino Jefferson Ribeiro da Silva, Quinn Matthews, Séverine Cazaux, Justin R. J. Vigar, Bárbara N. R. Santos, Deyse C. M. Carvalho, Lauren A. Cranmer, David Duplat, Serena Singh, Mohammad Simchi, et al.silencing, was used to improve linear DNA stability in the resulting lysates. E. coli NEB 5-alpha (NEB, C2987I) was used for plasmid cloning and purification. Bacterial strains were grown in Luria broth medium (except

(6) ErbB family receptor dimerization dynamics and dysregulation via long-term single-molecule imagingCellMay 28, 2026Kaibo Ma, Xiaojie Ma, João F. Shida, Zijian Niu, Yuzhu Karlie Lin, Saptarshi Mandal, Alexandra Dobbins, Lior Golomb, Michael J. Eck, Heidi Greulich, et al.virus strains,Bacterial and virus strains,Bacterial and virus strains DH5ɑ,New England BioLabs,Cat#: C2987H BL21(DE3) Competent E. coli,New England BioLabs,Cat#: C2521H "Chemicals, peptides, and recombinant

(7) Human pancreatic progenitor organoids define genetic and epigenetic barriers to early PDAC transformationDev CellMay 19, 2026Xian Zhang, Wilfred Wong, Hyein S. Cho, Dapeng Yang, Gary Dixon, Renhe Luo, Dingyu Liu, Denis Torre, Shigeaki Umeda, Federico González, et al.strains,Bacterial and virus strains,Bacterial and virus strains 5-alpha Competent E. coli,NEB,Cat# C2987H Biological samples,, OCT-embedded patient PDAC tumors,MSKCC,N.A. "Chemicals, peptides, and recombinant

(8) Cellular-state control using ribozyme-scaffolded miRNA-sensing and CRISPR-mediated actuationCell Reports MethodsMay 18, 2026Taek Kang, Leonidas Blerisintermediate and final plasmids were transformed into NEB-5alpha competent Escherichia coli (NEB, catalog #C2987H). Plasmid integrity was confirmed with direct Sanger sequencing, which was carried out by Genewiz, and

(9) A role for CASM in the repair of damaged Golgi architectureAutophagyMay 18, 2026Seeun Oh, Saif Ullah, Bhaskar Saha, Michael A Mandellstrains,Bacterial and virus strains NEB 5-alpha Competent E. coli (High Efficiency),New England Biolabs,C2987 XL10-Gold Ultracompetent cells,Agilent Technologies,210518 "Chemicals, peptides, and recombinant protein","Chemicals,

(10) ISGylation is disrupted by UBA7 gene variants identified in individuals with neurodevelopmental disorder phenotypesiScienceMay 15, 2026Venkateshwarlu Bandi, Myrrhe Venema, Iona Wallace, Merel O. Mol, Anita Nikoncuk, Rachel Schot, Marjon van Slegtenhorst, Emilia K. Bijlsma, Amjad Khan, Susan M. White, et al.(E. coli HST08),Takara Bio,Cat# 636763 NEB 5-alpha Competent E. coli (DH5α),New England Biolabs,Cat# C2987 BL21-CodonPlus (DE3)-RIPL Competent Cells (E. coli),Agilent Technologies,Cat# 230280 ,, "Chemicals,

(11) Structure and function of IWS1 in transcription elongationNucleic Acids ResMay 15, 2026Della Syau, Felix Steinruecke, Sophie Roth, Ernst Schmid, Karen Adelman, Johannes C Walter, Lucas Farnungprotein expression, unless otherwise indicated. Escherichia coli DH5α (New England Biolabs (NEB) Cat# C2987H), DH10EMBacY (Geneva Biotech), and BL21-CodonPlus (DE3)-RIL (Agilent Cat# 230245) were cultured in LB broth

(12) Natural xanthones as α-Mangostin induce vasorelaxation involving key gating residues in the S6 domain of BK channelsElifeMay 6, 2026Soenke Cordeiro, Robert Patejdl, Thomas Baukrowitz, Marianne A Musinszki(synthetic),EYFP,Addgene,https://www.addgene.org/vector-database/2688/,pEYFP "Strain, strain background (Escherichia coli)",DH5α,NEB,Cat. #: C2987,Chemically competent cells "Strain, strain background (Mus musculus)","CD1/CHR2 Mice (1 female, 7 male)",IMSR,RRID:IMSR_CRL:022,age

(13) Restoring the 14-3-3/CRAF regulatory interaction in Noonan syndrome using molecular gluesProceedings of the National Academy of SciencesMay 5, 2026Johanna M. Virta, Holly R. Vickery, Markella Konstantinidou, Mckenna C. Crawford, Marloes A. M. Pennings, Christian Ottmann, Luc Brunsveld, Michelle R. Arkinsite-directed mutagenesis (NEB KLD enzyme kit M0554) and expressed in NEB 5α competent Escherichia coli (NEB C2987). Protein Expression and Purification. 14-3-3σ was expressed and purified as previously described

(14) Massively parallel reporter assays for CYP3A4 enhancer variants alongside their native promoterbioRxivMay 1, 2026Yelena Guttman, Beniamin Krupkin, Nadav Ahituvassembled pGL4-GFP product was transformed into E. coli DH5α competent cells (New England Biolabs, C2987) and positive clones were validated by plasmid sequencing (Plasmidsaurus). Fragment cloning The XREM,

(15) INS-17 acts as a nutrient deprivation signal to mediate adult IIS-regulated associative behaviors in C. elegansPLOS GeneticsApril 28, 2026Emily J. Leptich, Priyadharshini Vijayakumar, Edward W. Pietryk, Meredith I. Williams, Rachana Rajupalem, Rachel N. Areyfor transformation. Competent cells were transformed using the New England Biolabs protocol (C2987H/C2987I). Single colonies of transformed cells were isolated and cultured in 3.0 mL of LB containing 50 μg/mL

(16) Decreasing peptide deformylase activity is a beneficial strategy for increasing formaldehyde resistance in Methylobacterium extorquensbioRxivApril 25, 2026Chandler N. Hellenbrand, Tyler Miller, Elias I. Kemna, Eric L. Bruger, Zachary T. Hying, Jannell V. BazurtoAssembly reaction at 50°C for 1 hr. Assemblies were transformed into NEB® 5-alpha Competent E. coli (C2987). Conjugation of plasmids into M. extorquens strains Cells were grown and exposed to E. coli strains

(17) Impact of dipeptidyl peptidase I and neutrophil serine proteases on neutrophil functional responsesFrontiers in PharmacologyApril 24, 2026Dedong Li, Kuan-Ju Chen, Nandita Niranjan, Jessica Basso, Daniel LaSala, Mei-Fong Pang, Craig Smith, Tam Nguyen, Vanessa de Carvalho Oliveira, David C. Cipolla, et al.Synergy Neo reader (Agilent Technologies). Bacterial killing assays Escherichia coli (DH5α; NEB #C2987H) were grown to exponential phase and quantified using an OD600-CFU standard curve. For neutrophil killing

(18) A multi-tetracycline responsive induction system for gene expression in Bacillus subtilisApplied and Environmental MicrobiologyApril 23, 2026Keira Reich-Veillette, Amrita E. Rhoads, Elizabeth A. Libbyordering gene synthesis through Azenta. All plasmids were cloned and propagated in E. coli (NEB DH5α, C2987H) and successful strains selected for on LB Lennox agar (Fisher LB agar 50-488-752, casein peptone 10

(19) SPNS2 exports sphingosine-1-phosphate and imports glucoseNat CommunApril 20, 2026Cynthia Weigel, Md Lokman Hossen, Ryan D. R. Brown, Can E. Senkal, Christopher D. Green, Jason Newton, Sumit Saha, Elisa N. D. Palladino, Bin Ni, Francesco S. Celi, et al.expression vector pEU-E01-MCS. Plasmid DNA template was isolated from transformed NEB 5-alpha E. coli (# C2987H, New England Biolabs) in LB broth with 100 µg/mL ampicillin (#L8105, Teknova) with the Monarch Plasmid Miniprep Kit

(20) Polarized SOS activity orchestrates FGF-directed cell migration in vivoCurrent BiologyApril 20, 2026Theresa V. Gibney, Laila Y. Latifi, Jacob I. Mardick, Neal R. Rasmussen, Maria C. Lyons, Meera V. Sundaram, David J. Reiner, Ariel M. PaniTechnologies (IDT). Constructs were transformed into chemically competent E. coli 5-alpha cells (NEB C2987). Plasmid DNA was extracted using either Qiagen (Qiagen 27104) or Invitrogen PureLink HQ (Invitrogen

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(1)

9/13/2018Competent E. coli for Cloning Constructs with High EfficiencyDr. Thippeswamy SannasiddappaRouting cloning plasmid constructs of size less than 8 Kb constructed by restriction cloning were transformed into NEB 5 alpha high efficiency competent E. coli cells by heat shock method. After ... Read Review
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4/9/2018Transformation Of Expression Plasmid Constructs By Heat Shock Method Triveni MahendrappaI have used NEB 5-alpha high efficiency competent E. coli cells for transformation of expression plasmid of size 6.3 Kb by heat shock method. After transformation, transformed cells were recovered ... Read Review