NEB 10-beta Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemNEB 10-beta Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $249.00 Inquire
Catalog NumberC3019H
Quantity20 x 0.05 ml
GenotypearaD139 Δ(ara-leu)7697 fhuA lacX74 galK (Φ80 Δ(lacZ)M15) mcrA galU recA1 endA1 nupG rpsL Δ(mrr-hsdRMS-mcrBC)
ApplicationsDNA Manipulation
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
1 - 3 x 10⁹ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Single-cell transcriptomics identifies a p21-activated kinase important for survival of the zoonotic parasite Fasciola hepaticaiScienceAugust 21, 2026Oliver Puckelwaldt, Svenja Gramberg, Sagar Ajmera, Janine Koepke, Jamal Shamsara, Christos Samakovlis, Peter Kolb, Simone Haeberleinstrains,Bacterial and virus strains ,, NEB 10-beta Competent E. coli (High Efficiency),New England Biolabs,Cat#C3019H ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(2) Identification and characterization of quorum-quenching enzyme AiiF from Acidithiobacillus ferrooxidans and its application for biofilm disruptionApplied and Environmental MicrobiologyAugust 19, 2026Mallori A. Herishko, Hannah S. Zurier, Scott Bantaexpression vector into chemically competent Escherichia coli 10-beta cells (New England Biolabs, Cat. No. C3019H; Ipswich, MA, USA). Overnight cultures of transformed cells were inoculated 1:100 into LB broth supplemented

(3) Synonymous mutations in essential genes infrequently produce fitness effects in human cell linesNucleic Acids ResAugust 5, 2026Yiyun Rao, Ivan Sokirniy, Edward O'Brien, Justin Pritchardcloning. sgRNA library transformation One day before electroporation, inoculate 10-beta competent cells (NEB, C3019H) in 200 ml media (20 g Tryptone/l, 10 g Yeast Extract/l). After the cell reached OD ∼ 0.5–0.6, cells

(4) Gabija restricts phage circularization and DNA replicationCell Host & MicrobeJuly 16, 2026Alex Hong, Miaoxi Liu, Alexis Truta, Alexander Talaie, Gerald R. Smith, Joseph Bondy-DenomyRESOURCE,SOURCE,IDENTIFIER Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains SM10,NEB,C3019H DH5α,NEB,C2987H XL1-Blue,QB3 Macrolab,N/A PAO1 WT,J. Bondy-Denomy Lab,NC_002516.2 PA14 WT,J. Bondy-Denomy

(5) Inhibition of lagging strand replication by G-rich telomeric DNA and the shelterin subunit POT1The EMBO JournalJuly 9, 2026Ciara Leonard-Booker, Giulia Mazzucco, Charlotte E L Fisher, Karim Hussain, Tom D Deegan, Ylli Doksani, Max E Douglas5-alpha competent (high efficiency),New England Biolabs,C2987H E. coli 10-beta,New England Biolabs,C3019H E. coli BL21-CodonPlus (DE3)-RIL,Agilent Technology,230245 E. coli SURE2,Agilent Technology,200152 Spodoptera frugiperda

(6) 19F NMR probes of conformational change in a bifurcating electron transfer flavoproteinBiophysical JournalJuly 7, 2026Anurag Priyadarshi, Anne-Frances Miller(New England Biolabs, NEB Cat #C2529H) and propagation strains (NEB 10-beta competent E. coli, Cat #C3019H). Isolated colonies of the expression strain were grown overnight (32°C, 180 rpm) to obtain 10 mL overnight

(7) Combining machine learning and iterative experiments to keep pace with emerging viral variants of concernPLoS Comput BiolJune 17, 2026Thomas Sheffield, Ryan C. Bruneau, Stephen Won, Kenneth L. Sale, Brooke Harmon, Le Thanh Mai PhamC-terminal. These plasmids were transformed into and amplified in 10B Escherichia coli cells (NEB, C3019H), extracted via Plasmid Plus 96 miniprep kits (Qiagen) and sequence verified by Genewiz (Azenta Life

(8) Discovery of Kv3.1 channel inhibitors reveals VU0521426 as a state-dependent inactivator preferentially active against pathogenic gain-of-function mutantsAmerican Journal of Physiology-Cell PhysiologyJune 1, 2026Rahul U. Chandrappa, Vaishali Satpute Satpute Janve, Emily L. Days, Jerod S. Dentonby bacterial transformation into NEB 10-beta competent E. coli cells (New England Biolabs, Catalog # C3019H). Transformed cells were cultured at 37°C for 1 h with shaking (200 rpm). Transformed cells were plated

(9) A novel reaction-diffusion architecture for engineering self-organized patterns in mammalian cellsbioRxivMay 28, 2026Benjamin Swedlund, John J Danan, Ting-Xin Jiang, Soha Ben Ben Tahar, Kyle Poon, Pranav S Bhamidipati, Zachary A Kreiger, Sandra Murillo, Minnal Kunnan, Daniel J G Pearce, et al.assembly (NEB, E2621L). The assembly product was introduced into chemically competent bacteria (NEB, C3019H). Colonies were then grown overnight in the antibiotic corresponding to the resistance of the plasmid

(10) Tollip antagonizes ESCRT-III-mediated plasma membrane repair and cell recoveryCell RegenMay 19, 2026Julia M. Ferrick, Xinan Meng, Emily Morgan, Anthony Kalvi, Zhen Li, Chaoqun Li, Chao-Yuan Chang, Suhong Xu, Yi-Nan GongCat No. 28,706), and ligated using T4 DNA Ligase (NEB, Cat No. M0202S). Competent cells (NEB, Cat No. C3019H) were transformed with ligation products, and colonies were screened by PCR using DreamTaq Green PCR

(11) Discovery of a chimeric transposase-transposon system for advanced genome engineeringiScienceMay 15, 2026Daniel Heinzelmann, Franziska Reuss, Nikolas Zeh, Robin Nilson, Emely Walker, Juergen Fieder, Benjamin Lindner, Benjamin Renner, Patrick Schulz, Simon Fischer, et al.strains ,, rAAV2-GFP vector,Boehringer proprietary,N/A NEB® 10-beta Competent E. coli,New England Biolabs,Cat# C3019H ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(12) Engineering Murine Cross‐Reactivity Into an Affibody to Human Death Receptor 5Biotechnology and BioengineeringMay 6, 2026Tse‐Han Kuo, Nagamani Vunnam, Jonathan N. Sachs, Benjamin J. Hackelfor DNA Amplification and Protein Production NEB 10‐beta competent Escherichia coli (E. coli) (NEB C3019H) were used to amplify the plasmid DNA. BL21(DE3) competent E. coli (NEB C2527H) was utilized to produce

(13) Systematic evaluation of the impact of promoter proximal short tandem repeats on expressionbioRxivApril 28, 2026Xuan Zhang, Lingzhi Zhang, Ellice Wang, Susan Benton, Eduardo Modolo, Mikhail Maksimov, Sharona Shleizer-Burko, Qingqing Gong, Catherine Wang, Michael Lamkin, et al.libraries, 4 μL of the ligated vector was transformed into 50μL of NEB10-beta competent cells (NEB C3019H) using heat shock transformation. For hSTR2, 1.5 μL of the purified ligated vector was electroporated

(14) Phaeochromocytomas and paragangliomas harbour tumour-initiating SOX2+ stem cellsEndocr Relat CancerApril 22, 2026Yasmine Kemkem, Mark Quinn, Bence Kövér, Alice Santambrogio, James Kaufman-Cook, Olivia Sherwin, Laura D Scriba, Dimitria Brempou, Miriam Vazquez Segoviano, Dylan Cameron, et al.plasmid was transformed into NEB® 10-beta Competent E. coli (High Efficiency; New England Biolabs, C3019H). Positive clones were screened, and the final construct was verified by Sanger sequencing. Transfection

(15) Intranasal Replicating Adenovirus type 4-Vectored SARS-CoV-2 Vaccines Induce Durable and Efficacious Responses in Preclinical TestingiScienceApril 17, 2026Breanna Kim, Rachel Roenicke, Peyton M. Roeder, Isabel Steinberg, Andy Patamawenu, Mitra Harrison, Freya van’t Veer, Emma Koory, Jane Xie, Tulley Shofner, et al.,Agilent Technologies,Cat#200154 E .coli DH10B,Thermo Fisher Scientific,Cat#EC0113 E .coli NEB 10 beta,New England Biolabs,Cat#C3019H Ad4-Luc,This study,N/A Ad4-H5,"Matsuda et al.25,26","Reference [25,26]" Ad4-Anc,This study,N/A Ad4-Beta,This

(16) Active Site Structure and Mechanism of a Molybdenum Catechol DehydroxylaseJournal of the American Chemical SocietyApril 15, 2026Jing Yang, Minwoo Bae, Khadanand KC, Xueyang Dong, Quyen D. Nguyen, Joshua Mengell, Emily P. Balskus, Martin L. Kirk(BHIrcf) unless otherwise noted. Bacterial Strains 10-beta competent E. coli (New England Biolabs, C3019H) was routinely used for cloning and DNA construction. Gordonibacter urolithinfaciens DSM 27213 (G. uro)

(17) Genotype-by-inhibitor interactions to dissect enterovirus replicationNature CommunicationsApril 11, 2026William Bakhache, Walker Symonds-Orr, Patrick T. Dolanassembled construct was then transformed into NEB 10-beta Chemically Competent E. coli (High Efficiency) (C3019H; New England Biolabs). Transformants were selected on LB agar containing 100 μg/mL carbenicillin, and

(18) Dissecting the homeodomain MAT locus and engineering novel tripolar and bipolar mating systems in Cryptococcus amylolentusmBioApril 8, 2026Liping Xiong, Vikas Yadav, Sheng Sun, Joseph Heitmanas template. DNA assembly product was transformed to NEB 10-beta competent E. coli cells (Cat. no. C3019H). Schemes of deletion cassettes are shown in Fig. S2B. For each strain construction, three DNA products,

(19) MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmyNat Struct Mol BiolApril 1, 2026Stephen P. Burr, Kathryn Auckland, Angelos Glynos, Abhilesh Dhawanjewar, Cameron Ryall, Wei Wei, Antony Hynes-Allen, Malwina Prater, Matylda Sczaniecka-Clift, Julien Prudent, et al.oligo was cloned into the LentiCRISPRv2 backbone96 and transformed in 10-beta competent E. coli (NEB, C3019H); then, plasmid DNA was extracted using the QIAprep spin miniprep kit (Qiagen, 27104). Single gRNA sequences

(20) Dependence of cell fate potential and cadherin switching on the coordinate within the primitive streak during differentiation of human pluripotent stem cellsDevelopmentApril 1, 2026Ye Zhu, Aryeh WarmflashmCerulean-CAAX plasmid (denoted AW-P235) was then transformed using NEB 10-beta competent Escherichia coli C3019H. DNA nucleofection All DNA nucleofections were performed using Amaxa P3 Primary Cell 4D-Nucleofector

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(1)

8/14/2023Reliable E. coli Competent Cells From New England BioLabs Pavan Kumar ChodisettiI have constructed a relatively large shuttle vector that can be propagated in both Gram-positive (E. faecium) and Gram-negative bacteria (E. coli). I used to have trouble transforming this plasmid ... Read Review