JM109 Competent Cells, >10(8)cfu/ug from Promega

Product Specs
ItemJM109 Competent Cells, >10(8)cfu/ug
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL2001
Quantity1ml
GenotypeendA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, Δ( lac-proAB), [F´ traD36, proAB, laqIqZΔM15].
Validated Species ReactivityEscherichia
Cell Type (standardized)Competent Cells

Specifications/Features

Transformation Efficiency:
>10⁸cfu/μg

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Dual EZH1/2 inhibition enhances DNMT inhibitor efficacy in colon cancer through targeting H3K27me1bioRxivSeptember 22, 2025Alison A. Chomiak, Ashley K. Wiseman, Joel Hrit, Yanqing Liu, Stephanie Stransky, Simone Sidoli, Rochelle L. Tiedemann, Scott B. Rothbartamplicon into a pGEM-T vector (Promega A1360), and transformed the plasmid into JM109 cells (Promega L2001) according to manufacturer’s guidelines. Plasmid DNA was amplified directly from bacterial colonies

(2) Development and Validation of a Standardized Pseudotyped Virus-Based Neutralization Assay for Assessment of Anti-Nipah Virus Neutralizing Activity in Candidate Nipah VaccinesVaccines (Basel)July 15, 2025Muntasir Alam, Md Jowel Rana, Asma Salauddin, Emma Bentley, Gathoni Kamuyu, Dipok Kumer Shill, Shafina Jahan, Mohammad Mamun Alam, Md Abu Raihan, Mohammed Ziaur Rahman, et al.These plasmids were transformed into competent E. coli JM109 cells (Promega, Madison, WI, USA, cat no. #L2001) and then plasmid DNA was extracted using a Qiagen midiprep kit (Qiagen, Hilden, Germany, cat. #12145)

(3) PRKACB is a novel imprinted gene in marsupialsEpigenetics & ChromatinSeptember 28, 2024Trent Newman, Donna M. Bond, Teruhito Ishihara, Phoebe Rizzoli, Quentin Gouil, Timothy A. Hore, Geoff Shaw, Marilyn B. Renfreevector cloning system (cat. no. A1360, Promega, Madison, WI, USA). JM109 competent cells (cat. no. L2001, Promega, Madison, WI, USA) were transformed with the ligation products and plated onto LB/Amp/IPTG/X-Gal.

(4) The structure of the TH/INS locus and the parental allele expressed are not conserved between mammalsHeredity (Edinb)July 1, 2024Trent Newman, Teruhito Ishihara, Geoff Shaw, Marilyn B. RenfreeEasy Vector System (cat. no. A1360, Promega, Madison, WI, USA) and JM109 Competent Cells (cat. no. L2001, Promega, Madison, WI, USA). Plasmids were extracted using the Wizard Plus SV Minipreps DNA Purification System

(5) The Evolution of Transglutaminases Underlies the Origin and Loss of Cornified Skin Appendages in VertebratesMolecular Biology and EvolutionJune 1, 2024Attila Placido Sachslehner, Marta Surbek, Karin Brigit Holthaus, Julia Steinbinder, Bahar Golabi, Claudia Hess, Leopold Eckhartligated into a pGEM-T Easy vector (Promega). Plasmids containing an insert were amplified in E. coli (L2001, Promega), and plasmid DNA was isolated with the QIAprep spin miniprep kit 250 (Qiagen). The plasmids

(6) Evolutionary origin of Hoxc13-dependent skin appendages in amphibiansNat CommunMarch 18, 2024Marjolein Carron, Attila Placido Sachslehner, Munevver Burcu Cicekdal, Inge Bruggeman, Suzan Demuynck, Bahar Golabi, Elfride De Baere, Wim Declercq, Erwin Tschachler, Kris Vleminckx, et al.pGEM-T Easy vector (catalog number: A1380, Promega) which was amplified in E. coli (catalog number: L2001, Promega). Using the same primers, hoxc13 was also amplified from toe cDNAs of wildtype frogs and frogs

(7) Evolution of two distinct variable lymphocyte receptors in lampreys: VLRD and VLRECell ReportsAugust 4, 2023Sabyasachi Das, Thomas Boehm, Stephen J. Holland, Jonathan P. Rast, Francisco Fontenla-Iglesias, Ryo Morimoto, J. Gerardo Valadez, Ryan D. Heimroth, Masayuki Hirano, Max D. Cooperstrains,, ,, Chemically Competent E. coli,Invitrogen,Cat# K287520 JM109 Competent cells,Promega,Cat# L2001 ,, Biological samples,, ,, "Blood, Kidney, Typhlosole, Intestine, Gill, and Skin from sea lamprey

(8) Restricting genomic actions of innate immune mediators on fetal hematopoietic progenitor cellsiScienceApril 21, 2023Vu L. Tran, Peng Liu, Koichi R. Katsumura, Erin Kim, Bjorn M. Schoff, Kirby D. Johnson, Emery H. Bresnickstrains,Bacterial and virus strains,Bacterial and virus strains ,, JM109 competent cells,Promega,L2001 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(9) Discriminating activities of DEAD-Box Helicase 41 from myeloid malignancy-associated germline variants by genetic rescueLeukemiaJanuary 1, 2023Kim JA, Shen S, Matson DR, Lovrien LN, Smith-Simmer KJ, Keles S, Churpek JE, Bresnick EH.Reagent,Source,Identifier Bacterial strains,Bacterial strains,Bacterial strains JM109 competent cells,Promega,L2001 Experimental model: Cell lines,Experimental model: Cell lines,Experimental model: Cell lines Mouse:

(10) Protocol: A Multiplexed Reporter Assay to Study Effects of Chromatin Context on DNA Double-Strand Break RepairFrontiers in GeneticsJanuary 31, 2022Ruben Schep, Christ Leemans, Eva K. Brinkman, Tom van Schaik, Bas van SteenselCloneCatcher DH5α electrocompetent E. coli (Genlantis—Cat# C810111)2. JM109 competent cells (Promega—Cat#: L2001) 5.2.4 Commercial Kits 1. PCR Isolate II PCR and Gel Kit (Bioline, Cat#: BIO-52060; brand not critical)2.

(11) HES and Mox genes are expressed during early mesoderm formation in a mollusk with putative ancestral featuresScientific ReportsDecember 1, 2021Attila Sachslehner, Elisabeth Zieger, Andrew Calcino, Andreas WanningerpGEM-T easy vector (Promega #A1380). The plasmid was amplified using E. coli competent cells (Promega #L2001). Plasmid DNA was purified using the QIAprep spin miniprep kit 250 (Qiagen #27106). Inserts were sequenced

(12) Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balanceMolecular cellMay 20, 2021Schep R, Brinkman EK, Leemans C, Vergara X, van der Weide RH, Morris B, van Schaik T, Manzo SG, Peric-Hupkes D, van den Berg J, et al.strains ,, CloneCatcher DH5α electrocompetent E. coli,Genlantis,Cat# C810111 JM109 Competent Cells,Promega,Cat# L2001 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(13) RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cellseLifeDecember 2, 2020Tullett KM, Tan PS, Park HY, Schittenhelm RB, Michael N, Li R, Policheni AN, Gruber E, Huang C, Fulcher AJ, et al.reference,Identifiers,Additional information "Strain, strain background (Escherichia coli)",JM109,Promega,Cat# L2001,Competent cells "Strain, strain background (Escherichia coli)",BL21(DE3)pLysS,Promega,Cat# L1195,Competent

(14) Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 PhosphorylationCell reportsSeptember 15, 2020Chen K, Jiao X, Di Rocco A, Shen D, Xu S, Ertel A, Yu Z, Di Sante G, Wang M, Li Z, et al.Virus Strains,Bacterial and Virus Strains,Bacterial and Virus Strains JM109 Competent Cells,Promega,L2001 "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(15) Discovering How Heme Controls Genome Function Through Heme-omicsCell ReportsJune 30, 2020Ruiqi Liao et al.REAGENT or RESOURCE,SOURCE,IDENTIFIER Bacterial and Virus Strains,, JM109 competent cells,Promega,L2001 Biological Samples,, G-CSF-mobilized human peripheral blood,University of Wisconsin-Madison,N/A "Chemicals,

(16) Paradoxical Role for Wild-Type p53 in Driving Therapy Resistance in MelanomaMolecular cellFebruary 6, 2020Webster MR, Fane ME, Alicea GM, Basu S, Kossenkov AV, Marino GE, Douglass SM, Kaur A, Ecker BL, Gnanapradeepan K, et al.[EPR18021],Abcam,Ab188224 Bacterial and Virus Strains,, JM109 High Efficiency Competent Cells,Promega Corp.,Cat# L2001 Biological Samples,, Patient-derived xenografts (PDX) PFFE blocks,Herlyn Lab at Wistar,"WM4351, WM4298,

(17) Ligand Modulates Cross-Coupling between Riboswitch Folding and Transcriptional PausingMolecular CellNovember 1, 2018Julia R. Widom, Yuri A. Nedialkov, Victoria Rai, Ryan L. Hayes, Charles L. Brooks, Irina Artsimovitch, Nils G. WalterStrains,Bacterial and Virus Strains,Bacterial and Virus Strains E. coli: JM109 competent cells,Promega,Cat#L2001 E. coli: BLR (DE3) competent cells,EMD Millipore,Cat#69053 E. coli: XJb (DE3) competent cells,Zymo Research

(18) TOR-autophagy branch signaling via Imp1 dictates plant-microbe biotrophic interface longevityPLoS geneticsNovember 1, 2018Sun G, Elowsky C, Li G, Wilson RA.Afterward, 10 μL of the ligation products were transformed into the competent E. coli (JM-109; Promega, L2001) for plasmid amplification. Plasmids were extracted from a 3 mL culture of the transformed JM-109 strain

(19) Assaying the Effects of Splice Site Variants by Exon Trapping in a Mammalian Cell LineBio-protocolMay 20, 2017Tompson SW, Young TL.Scientific, Invitrogen™, catalog number: AM12275) JM109 E. coli competent cells (Promega, catalog number: L2001) pSPL3 vector (Thermo Fisher Scientific, Invitrogen) Restriction endonuclease, XhoI (New England Biolabs

(20) RAB10 Interacts with the Male Germ Cell-Specific GTPase-Activating Protein during Mammalian SpermiogenesisInternational journal of molecular sciencesJanuary 5, 2017Lin YH, Ke CC, Wang YY, Chen MF, Chen TM, Ku WC, Chiang HS, Yeh CH.T4 DNA ligase (M0202; New England Biolabs, Ipswich, MA, USA) and JM109 competent cells, respectively (L2001; Promega, Fitchburg, WI, USA) [15,16]. The construct was confirmed via sequencing. Subsequently, NTERA-2

Be the First to Write a Review!