Single Step (KRX) Competent Cells from Promega

Product Specs
ItemSingle Step (KRX) Competent Cells
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL3002
Quantity20 x 50microl
GenotypeGenotype: [F-prime, traD36, -delta-ompP, proA+B+, lacI^q, -delta-(lacZ)M15] -delta-ompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (r_k-, m_k+), e14- (McrA-), relA1, supE44, -delta-(lac-proAB), -delta-(rhaBAD)::T7 RNA polymerase.
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia

Specifications/Features

Transformation Efficiency:
10E8 cfu/microg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Structural basis of RNAPII transcription on the nucleosome containing histone variant H2A.BThe EMBO JournalMay 30, 2025Munetaka Akatsu, Rina Hirano, Tomoya Kujirai, Mitsuo Ogasawara, Haruhiko Ehara, Shun-ichi Sekine, Yoshimasa Takizawa, Hitoshi Kurumizaka(DH5α),Takara,Cat#9057 E. coli (NEB Turbo),New England Biolabs,Cat#C2984I E. coli (KRX),Promega,Cat#L3002 K. phaffi (THY46 strain),"Higo et al, 2014",N/A Recombinant DNA,Recombinant DNA,Recombinant DNA pET-15b-H2A,"Machida

(2) Structural and molecular basis of PCNA-activated FAN1 nuclease function in DNA repairNat CommunMay 14, 2025F. Li, A. S. Phadte, M. Bhatia, S. Barndt, A. R. Monte Carlo Iii, C-F. D. Hou, R. Yang, S. Strock, A. PluciennikFAN1_R507A and FAN1_4A were expressed and purified from E. coli Single step (KRX) cells (Promega Cat# L3002) harboring plasmid pET28a-His-MBP-FAN1 or plasmids expressing the mutant forms of FAN1 as described

(3) Heme regulates protein interactions and phosphorylation of BACH2 intrinsically disordered region in humoral responseiScienceJanuary 17, 2025Miki Watanabe-Matsui, Shun Kadoya, Kei Segawa, Hiroki Shima, Tadashi Nakagawa, Yuko Nagasawa, Shuichiro Hayashi, Mitsuyo Matsumoto, Mariko Ikeda, Akihiko Muto, et al.virus strains,Bacterial and virus strains,Bacterial and virus strains ,, E. coli: Strain: KRX,Promega,L3002 ,, Biological samples,Biological samples,Biological samples ,, His-FLAG-DNMT1,BPS Bioscience,Cat#51100 ,, "Chemicals,

(4) Structural basis of 3′-tRNA maturation by the human mitochondrial RNase Z complexThe EMBO JournalNovember 8, 2024Genís Valentín Gesé, B Martin Hällbergor catalog number Experimental models,Experimental models,Experimental models E. coli KRX,Promega,L3002 Recombinant DNA,Recombinant DNA,Recombinant DNA pNIC-CTHF-M21,"Reinhard et al, 2017",N/A pJ411,DNA

(5) Voltage sensors of a Na+ channel dissociate from the pore domain and form inter-channel dimers in the resting stateNat CommunDecember 19, 2023Ayumi Sumino, Takashi Sumikama, Mikihiro Shibata, Katsumasa Iriewere confirmed by DNA sequencing. Proteins were expressed in the Escherichia coli KRX strain (Promega; L3002). Cells were grown at 37 °C to an OD600 of 0.6, induced with 0.2% α-L (+)-Rhamnose Monohydrate (Fujifilm

(6) The structural basis of divalent cation block in a tetrameric prokaryotic sodium channelNature CommunicationsJuly 15, 2023Katsumasa Irie, Yoshinori Oda, Takashi Sumikama, Atsunori Oshima, Yoshinori FujiyoshiDNA sequencing. Protein expression and purification Proteins were expressed in the Escherichia coli KRX strain (Promega; L3002). Cells were grown at 37 °C to an OD600 of 0.6, induced with 0.2% α-L (+)-Rhamnose Monohydrate (Fujifilm

(7) (α)N-Acetyl β-Endorphin Is an Endogenous Ligand of σ1Rs That Regulates Mu-Opioid Receptor Signaling by Exchanging G Proteins for σ2Rs in σ1R OligomersInternational journal of molecular sciencesDecember 29, 2022Garzón-Niño J, Cortés-Montero E, Rodríguez-Muñoz M, Sánchez-Blázquez P.proteins were identical to the GenBank™ sequences. The vector was introduced into the E. coli BL21 (KRX #L3002, Promega) and clones were selected on solid medium containing ampicillin. After 3 h of induction at

(8) Phosphorylated MED1 links transcription recycling and cancer growthNucleic Acids ResearchMay 6, 2022Zhong Chen et al.verified by DNA sequencing. The resulting plasmids were then transfected into KRX competent cells (Cat #L3002, Promega) and expression of GST–MED4, GST–MED9 and GST–MED31 protein fusions was induced by 0.1% rhamnose

(9) A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivoCell Reports MethodsFebruary 28, 2022Masayuki Sakamoto, Masatoshi Inoue, Atsuya Takeuchi, Shigetaka Kobari, Tatsushi Yokoyama, Shin-ichiro Horigane, Sayaka Takemoto-Kimura, Manabu Abe, Kenji Sakimura, Masanobu Kano, et al.Fisher Scientific) at 28°C. E. Coli DH5α and KRX cells were obtained from TOYOBO (DNA-903) and Promega (L3002), respectively. Bacteria were incubated in Lysogeny Broth medium supplemented with antibiotics at 37°C.

(10) The σ1 Receptor and the HINT1 Protein Control α2δ1 Binding to Glutamate NMDA Receptors: Implications in Neuropathic PainBiomoleculesNovember 12, 2021Rodríguez-Muñoz M, Cortés-Montero E, Onetti Y, Sánchez-Blázquez P, Garzón-Niño J.identical to the GenBank™ sequences when sequenced. The vector was introduced into the E. coli BL21 (KRX #L3002, Promega, Madison, WI, USA) and clones were selected on solid medium containing ampicillin. After a

(11) Novel Phage-Derived Depolymerase with Activity against Proteus mirabilis BiofilmsMicroorganismsOctober 19, 2021Cormac J. Rice, Stephen A. Kelly, Seamus C. O’Brien, Erinn M. Melaugh, Jan C. B. Ganacias, Zheng Hua Chai, Brendan F. Gilmore, Timofey Skvortsovthis solution was used to transform Escherichia coli KRX cells (Promega, Madison, WI, USA, cat. no. L3002) using heat shock transformation. Briefly, 5 µL of the plasmid was added to 50 µL of KRX competent cells

(12) Human HINT1 Mutant Proteins that Cause Axonal Motor Neuropathy Exhibit Anomalous Interactions with Partner ProteinsMolecular NeurobiologyApril 1, 2021Elsa Cortés-Montero, María Rodríguez-Muñoz, Pilar Sánchez-Blázquez, Javier Garzón-Niñothey were identical to the GenBank™ sequences. The vector was introduced into the E. coli BL21 (KRX #L3002, Promega) and clones were selected on solid medium containing ampicillin. After 3 h of induction at

(13) Calmodulin Supports TRPA1 Channel Association with Opioid Receptors and Glutamate NMDA Receptors in the Nervous TissueInternational journal of molecular sciencesDecember 28, 2020Cortés-Montero E, Rodríguez-Muñoz M, Ruiz-Cantero MDC, Cobos EJ, Sánchez-Blázquez P, Garzón-Niño J.proteins were identical to the GenBank™ sequences. The vectors were introduced into E. coli BL21 (KRX #L3002, Promega) and clones were selected on solid medium containing ampicillin. After 3 h of induction at

(14) The ALS-related σ1R E102Q Mutant Eludes Ligand Control and Exhibits Anomalous Response to CalciumInternational journal of molecular sciencesOctober 4, 2020Rodríguez-Muñoz M, Cortés-Montero E, Garzón-Niño J, Sánchez-Blázquez P.were identical to the GenBank sequences. The vectors were introduced into Escherichia coli BL21 (KRX #L3002, Promega), and clones were selected on solid medium containing ampicillin. After 3 h of induction at

(15) Dinucleotide Degradation by REXO2 Maintains Promoter Specificity in Mammalian MitochondriaMolecular CellDecember 5, 2019Thomas J. Nicholls, Henrik Spåhr, Shan Jiang, Stefan J. Siira, Camilla Koolmeister, Sushma Sharma, Johanna H.K. Kauppila, Min Jiang, Volkhard Kaever, Oliver Rackham, et al.Strains ,, Rosetta 2 Competent Cells,Merck Millipore,Cat#71402 Single Step (KRX) Competent Cells,Promega,Cat#L3002 Autographa californica nuclear polyhedrosis virus,Clontech,Cat#631401 ,, "Chemicals, Peptides, and

(16) The Axonal Motor Neuropathy-Related HINT1 Protein Is a Zinc- and Calmodulin-Regulated Cysteine SUMO ProteaseAntioxidants & redox signalingSeptember 1, 2019Cortés-Montero E, Rodríguez-Muñoz M, Sánchez-Blázquez P, Garzón J.were identical to the GenBank™ sequences. The vector was introduced into Escherichia coli BL21 (KRX #L3002; Promega), and clones were selected on solid medium containing ampicillin. After 3 h of induction at

(17) Distinct structural classes of activating FOXA1 alterations in advanced prostate cancerNatureJuly 1, 2019Parolia A, Cieslik M, Chu SC, Xiao L, Ouchi T, Zhang Y, Wang X, Vats P, Cao X, Pitchiaya S, et al.expression constructs were used to transform the Single Step (KRX) Competent E. coli cells (Promega, Cat#: L3002), which have been modified for synthesis of mammalian proteins. A starter broth of 2 ml was inoculated

(18) Ligands Exert Biased Activity to Regulate Sigma 1 Receptor Interactions With Cationic TRPA1, TRPV1, and TRPM8 ChannelsFrontiers in pharmacologyJanuary 1, 2019Cortés-Montero E, Sánchez-Blázquez P, Onetti Y, Merlos M, Garzón J.identical to the GenBank™ sequences. The vector was introduced into the Escherichia coli BL21 (KRX #L3002, Promega), and clones were selected on solid medium containing ampicillin. After 3 h of induction at

(19) Sequential Regulation of Maternal mRNAs through a Conserved cis-Acting Element in Their 3′ UTRsCell ReportsDecember 26, 2018Pooja Flora, Siu Wah Wong-Deyrup, Elliot Todd Martin, Ryan J. Palumbo, Mohamad Nasrallah, Andrew Oligney, Patrick Blatt, Dhruv Patel, Gabriele Fuchs, Prashanth RanganStrains,, ,, BL21(DE3) competent E.coli,New England Biolabs Inc.,Cat# C25271 KRX E.coli competent cells,Promega,Cat# L3002 DH5α competent cells,Invitrogen,Cat# 18265017 ,, "Chemicals, Peptides, and Recombinant Proteins",, ,, "Formaldehyde

(20) De novo mutations in the GTP/GDP-binding region of RALA, a RAS-like small GTPase, cause intellectual disability and developmental delayPLoS geneticsNovember 1, 2018Hiatt SM, Neu MB, Ramaker RC, Hardigan AA, Prokop JW, Hancarova M, Prchalova D, Havlovicova M, Prchal J, Stranecky V, et al.the coding regions of RALA were those observed in the probands. Single Step (KRX) Competent Cells (#L3002, Promega Corporation, Madison WI, USA) were transformed with plasmids, and bacteria were grown overnight

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