Description
The TLR3/NF-kB Leeporter GFP Reporter cell line is a stably transfected HEK293 cell line, which expresses full-length human Toll-like receptor 3 (TLR3) and enhanced green fluorescent protein (eGFP) reporter gene under the transcriptional control of the NF-kB response element. Functional activity of the cell line has been validated by TLR3 ligand assay, in which upon activation by poly (I:C), TLR3 quickly initiates TRIF-dependent signaling pathway and mediates nuclear translocation of NF-kB (Figures 1 and 2)