Description
BL21(DE3) Competent Cells and product number, BL21
E Coli BL21(DE3) Competent Cells are an all purpose reagent for T7 promoter driven IPTG inducible E Coli protein expression.
For greater transformation efficiencies heat shock at 37C for 1 minute before plating, or heat shock at 42C for 30 seconds then add SOC media and shake at 225 rpm at 37C for 1 hour before plating. Conveniently supplied as a pack of 10 single use 0.1 ml aliquots.
BL21(DE3) Competent Cells and product number, BL21