Description
The Immortalized Mouse Epidermal Cells (COCA) retains its capacity to differentiate and stratify in response to increased calcium concentration and can form three-dimensional in vitro models. They form epidermis in grafting assays in vivo. It is resourceful as skin modeling and can be used for 3D epidermal models appropriate for skin biology research. Keratinocyte differentiation can be induced by switching the media to high calcium (1.2 mM) containing media