A549 from ATCC

Product Specs
ItemA549
CompanyATCC
Price $714.00Supplier Page View Company Product Page
Catalog NumberCRM-CCL-185
TypeCell Biology
SpeciesHomo sapiens
TissueLung
Cell LineA-549
Tissue (standardized)Lung
Cell Typeepithelial cell
Age58 years
DiseaseCarcinoma
ApplicationsFor use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. This cell line may be used as a transfection host

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Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Differential KEAP1/NRF2 mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapyNat CommunApril 12, 2026Jana Samarin, Hana Nůsková, Piotr Fabrowski, Mona Malz, Eberhard Amtmann, Minerva J. Taeubert, Daniel Pastor-Flores, Daniel Kazdal, Roman Kurilov, Nicole de Vries, et al.(ATCC: NCI-H1944 CRL-5907) were cultivated in RPMI-1640 (Gibco). Other NSCLC cell lines (ATCC: A549 CRM-CCL-185 and NCI-H838 CRL-5844) were grown in low-glucose and high-glucose DMEM (Gibco), respectively. Resistance

(2) Discovery of Two Novel Pyrazole Derivatives as Anticancer Agents Targeting Tubulin Polymerization and MAPK Signaling PathwaysOncology ResearchMarch 23, 2026Denisse A. Gutierrez, Elisa Robles-Escajeda, Jose A. Lopez-Saenz, Robert A. Kirken, Edgar A. Borrego, Ana P. Betancourt, Soumya Nair, Sourav Roy, Armando Varela-Ramirez, Renato J. Aguilera(ATCC, TIB-152), NALM-6 (ATCC, CRL-3273), RPMI-8226, MM.1S, MM.1R, U266, Rec-1, JVM-13, A549 (ATCC, CRM-CCL-185), HCC70 (ATCC, CRL-2315), HCC1419 (ATCC, CRL-2326), T47D (ATCC, CRL-2865), KMS-11, and OVCAR-5 cell

(3) Kasugamycin Inhibits Melanoma Lung Metastasis and CHI3L1-Driven M2-Like Tumor-Associated Macrophage DifferentiationImmunotargets TherMarch 19, 2026Takayuki Sadanaga, Han-Seok Jeong, Roberto Cortez, Joyce H Lee, Suchitra Kamle, Bing Ma, Sung Jae Shin, Jack A Elias, Chun Geun LeeTAMs using recombinant (r) M-CSF, CHI3L1 along with tumor cell-conditioned media (from A549 cells (#CRM-CCL-185, ATCC)), following established procedures with modification.19 In this protocol, we replaced the traditional

(4) Intranasal DC-targeting vaccine booster elicits durable and cross-clade protective immunity against sarbecoviruses in miceJournal of Clinical InvestigationMarch 16, 2026You Zhi Nicholas CHEANG, Wee Chee Yap, Kirsteen McInnes TULLETT, Xinlei QIAN, Peck Szee TAN, Kiren PURUSHOTORMAN, Wan Yi TAN, Shirley Yun Yan MAH, Paul Anthony MACARY, Chee Wah TAN, et al.(Pen/Strep) (Thermo Fisher Scientific, 15140122) at 37°C and 5% CO2. Human lung alveolar A549 cells (ATCC, CRM-CCL-185) transduced in-house with lentivirus carrying human ACE2 gene in pFUGW vector (A549-ACE2) (provided

(5) Riboflavin metabolism shapes FSP1-driven ferroptosis resistanceNature Cell BiologyMarch 13, 2026Vera Skafar, Izadora de Souza, Biplab Ghosh, Ancely Ferreira dos Santos, Florencio Porto Freitas, Zhiyi Chen, Shibo Sun, Merce Donate Castillo, Palina Nepachalovich, Lars Seufert, et al.MDA-MB-436, A549 and H460 were purchased from ATCC (cat. nos. CCL-121, CRL-1619, CRM-HTB-26, HTB-130, CRM-CCL-185 and HTB-177, respectively) and PC-9 cells were purchased from Sigma (cat. no. 90071810). Cells were

(6) Extracellular vesicles of Emergomyces africanus modulate host immune responses and reflect metabolic adaptations to nutrient availabilityInfection and ImmunityMarch 10, 2026Leandro Honorato, Albaniza Liuane Ribeiro do Nascimento Sabino, Jhon Jhamilton Artunduaga Bonilla, Susana Ruiz Mendoza, Julio Kornetz, Flavia C. G. dos Reis, Elaine R. Albergoni, Vinicius Alves, Susana Frases, Allan Jefferson Guimarães, et al.alveolar macrophage cell line (ATCC AMJ2-C11—CRL-2456) and human lung adenocarcinoma cell line (ATCC A549—CRM-CCL185) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) High Glucose (Gibco, US) containing 10%

(7) Decreased Glucose Metabolism and Declined Chaperones Are Unique Features Required for the Survival of Senescent Fibroblasts and Pyruvate Dehydrogenase Is a Potent Senolytic TargetAging CellMarch 5, 2026Mingzhu Zhang, Ziqi Hu, Shengwen Piao, Yingrui Song, Ying Jia, Jiaxing Liu, Ning Zhao, An Liu, Songbin Fu, Wenjing Sun, et al.human cancer cell lines HeLa (RRID: CVCL_0030; ATCC Cat#CRM‐CCL‐2), A549 (RRID: CVCL_0023; ATCC Cat#CRM‐CCL‐185), and U2OS (RRID: CVCL_0042; ATCC Cat#HTB‐96) were purchased from American Type Culture Collection (ATCC)

(8) Aspartate availability drives differential engagement of the malate-aspartate shuttleMolecular CellMarch 5, 2026Julia S. Brunner, Anna E. Bridgeman, Benjamin T. Jackson, Sangita Chakraborty, Maider Fagoaga-Eugui, Katrina I. Paras, Abigail Xie, Paige K. Arnold, Julia Losner, Lydia W. S. Finleylines "C3H/10T1/2, Clone 8",ATCC,Cat#CCL-226 C2C12,ATCC,Cat#CRL-1772 293T,ATCC,Cat#CRL-3216 A549,ATCC,Cat#CRM-CCL-185 SW872,ATCC,Cat#HTB-92 Calu1,ATCC,Cat#HTB-54 NCI-H1975,ATCC,Cat#CRL-5908 NCI-H2122,ATCC,Cat#CRL-5985 NCI-H2170,ATCC,Cat#CRL-5928 NCI-H1993,ATCC,Cat#CRL-5909 Oligonucleotides,Oligonucleotides,Oligonucleotides Guide

(9) Development of immortalized Callithrix jacchus kidney cell lines supporting infection with a panel of virusesPLoS OneMarch 2, 2026Sabine Gärtner, Pamela Stomberg, Stefan Pöhlmann, Michael Winklercatalogue no. CRL-1586; RRID: CVCL 0574; kindly provided by Andrea Maisner) and A549 (human lung, male; CRM-CCL-185, ATCC, RRID: CVCL_0023; kindly provided by Benjamin tenOever) were cultured in DMEM supplemented with

(10) Wnt signaling is modulated by the buffer-like properties of cadherinsMolecular Biology of the CellMarch 1, 2026Lauren Schnitkey, Christin Anthony, Marina Cardó-Vila, Matthew A. Loberg, Gemma Orton, Allison McCabe, Jonathan Trapani, Mary Rockouski, Joyce Luo, Sheau-Chiann Chen, et al.humidified 5% CO2 environment. HEK293T (ATCC CRL-3216), HEK293TNC, ARPE-19 (ATCC CRL-2302), A549 (ATCC CRM-CCL-185), and SK-CO15 (obtained from Memorial Sloan Kettering Cancer Center) cells were cultured in DMEM supplemented

(11) Dihydro-R demonstrates innate immunity against Adenovirus-7 by suppressing the NF-κB/JAK-STAT pathway in a SIRT1-dependent mannerBiochemistry and Biophysics ReportsMarch 1, 2026Chenyang Wang, Xiaoshan Li, Changbing Wang, Yudan Ye, Mingqi Zhao, Min Guo, Tiantian Xu, Lu Kuang, Yuqing Yan, Wanli Liang, et al.adenovirus susceptible cells: A549 cells (Human Lung Adenocarcinoma Cell Line. ATCC# Product code: CRM-CCL-185), AD293 cells (Human Embryonic Kidney (HEK) cell line. ATCC# Product code: CRL-1573) and normal human

(12) Citrate clearance is a major function of aconitase 2 in the canonical TCA cycleCellFebruary 27, 2026Abigail Xie, Julia S. Brunner, Sangita Chakraborty, Angela M. Montero, Anna E. Bridgeman, Katrina I. Paras, Ruobing Cui, Maider Fagoaga-Eugui, Monika Komza, Paige K. Arnold, et al.lines "C3H/10T1/2, Clone 8",ATCC,Cat#CCL-226 C2C12,ATCC,Cat#CRL-1772 293T,ATCC,Cat#CRL-3216 A549,ATCC,Cat#CRM-CCL-185 Calu1,ATCC,Cat#HTB-54 NCI-H1975,ATCC,Cat#CRL-5908 NCI-H2122,ATCC,Cat#CRL-5985 NCI-H2170,ATCC,Cat#CRL-5928 NCI-H1993,ATCC,Cat#CRL-5909 HepG2,ATCC,Cat#HB-8065 Calu3,Gift

(13) The polyamine inhibitor SAM486A increases the efficacy of adagrasib in non-small cell lung cancer cells harboring KRASG12C mutationBiological ResearchFebruary 19, 2026Antonia Martin-Martin, Carina Chipón, Constanza Guzman-Kunstmann, Sergio Guzman-Kunstmann, Neudo Buelvas, Claudio Henríquez, Francisca Vidal, Franz Villarroel-Espindola, Trista K. Hinz, Lynn E. Heasley, et al.lines and culture method We utilized the human NSCLC cell lines H1299 (ATCC®CRL-5803™), A549 (ATCC®CRM-CCL-185™), CALU-1 (ATCC®HTB-54™), and H358 (ATCC®CRL-5807™). Also, we used the murine cell lines LL2 (Lewis Lung Carcinoma

(14) Ferroptosis induces heterogeneous death profiles that are controlled by lysosome ruptureDevelopmental CellFebruary 17, 2026Jyotirekha Das, Saloni K. Hombalkar, Alison D. Klein, Esraa Nsasra, Muskaan Vasandani, Kay Petruzzi, Dajun Lu, Stephen Ruiz, Orit Kliper-Gross, Jiachen Hu, et al.U2OS,ATCC,Cat#: HTB-96 Human: HeLa,ATCC,Cat#: CCL2 Human: HT-1080,ATCC,Cat#: CCL-121 Human: A549,ATCC,Cat#: CRM-CCL-185 Human: HaCaT,ADDEXBIO TECHNOLOGIES,Cat#: T0020001 Human: MCF10A GFP-Galectin-3 MCF10A,Overholtzer

(15) Enhanced genome replication activity of pandemic H1N1 influenza A virus through PA mutationsJournal of VirologyFebruary 17, 2026Jordana Schmierer, Michael Lutz, Toru Takimotogenome replication. MATERIALS AND METHODS Cell lines and plasmids MDCK (ATCC: CCL-34), A549 (ATCC: CRM-CCL-185), and 293T (ATCC: CRL-3216) cells were maintained in Dulbecco’s-modified Eagle’s medium (DMEM, Gibco)

(16) S6K1 Modulates STAT3 Activation to Promote Resistance to Radiotherapy in Lung CancerInternational Journal of Molecular SciencesFebruary 17, 2026Ali Calderon-Aparicio, Noelle Francois, Tyler Grenda, Shan Xu, Olugbenga Okusanya, Jun He, Nicole L. Simonelines H23 (ATCC#CRL-5800; RRID# RRID: CVCL_1547), H226 (ATCC#CRL-5826; RRID# CVCL_1544), A549 (ATCC#CRM-CCL-185; RRID# CVCL_0023), the large cell lung cancer cell line H661 (ATCC#HTB-183; RRID# CVCL_1577), and the

(17) dHyperCas12a enables multiplexed CRISPRi screensNature CommunicationsFebruary 12, 2026Schuyler M. Melore, Christian D. McRoberts Amador, Marisa C. Hamilton, Charles A. Gersbach, Timothy E. Reddyunchanged during the second round of cloning. Cell culture HEK293T (Cat. No. CRL-3216), A549 (Cat. No. CRM-CCL-185), and HepG2 (Cat. No. HB-8065) cell lines were obtained from ATCC. HEK293T and A549 cell lines were

(18) Cardenolide‐Engineered Extracellular Vesicles Augment Drug Uptake and Cytotoxicity in Non‐small Cell Lung Cancer CellsSmall MethodsJanuary 14, 2026Maja Dorfner, Anika Mifka, Rodrigo Maia de Pádua, Izabella Thaís da Silva, Iara Zanella Guterres, Lorenzo Sana, Gregor Fuhrmann, Jennifer Munkertspecified, additional chemicals were purchased from Sigma‐Aldrich or Carl Roth. 4.2 Cell Lines A549 (CRM‐CCL‐185) and MRC‐5 (CCL‐171) cells were purchased from the ATCC Cell Bank (ATCC, VA, USA). All cells were cultured

(19) Integrating Computational and Experimental Approaches for the Discovery of Multifunctional Peptides from the Marine Gastropod Pisania pusio with Antimicrobial and Anticancer PropertiesMarine DrugsJanuary 8, 2026Ernesto M. Martell-Huguet, Thalia Moran-Avila, José E. Villuendas, Armando Rodriguez, Ann-Kathrin Kissmann, Ludger Ständker, Sebastian Wiese, Anselmo J. Otero-Gonzalez, Frank RosenauCulture Conditions The human cancer cell lines, A549 (epithelial cell lung carcinoma, ATCC number CRM-CCL-185), A375 (malignant melanoma, ATCC number CRL-1619), MIA PaCa-2 (pancreatic carcinoma, ATCC number CRM-CRL-1420),

(20) Complete neutralizing antibody evasion by serodivergent non-mammalian AAVs enables gene therapy redosingCell Reports MedicineDecember 16, 2025Ezra J. Loeb, Sophia A. Fergione, Vivian Yudistyra, Marco M. Fanous, Abigail R. Benkert, Delaney G. Fisher, Joshua A. Hull, Mai K. ElMallah, Aravind AsokanLines,Experimental Models: Cell Lines,Experimental Models: Cell Lines ,, HEK293,ATCC,CRL-1573 A549,ATCC,CRM-CCL-185 U-87 MG,ATCC,HTB-14 RD,ATCC,CCL-136 C2C12,ATCC,CRL-1772 HuH7,CLS,300156 TH1,Kerafast,ECH001 ,, Experimental Models

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