RPTEC/TERT1 from ATCC

Product Specs
ItemRPTEC/TERT1
CompanyATCC
Price $8,670.00Supplier Page View Company Product Page
Catalog NumberCRL-4031
ApplicationsThese cells are proposed to be a valuable model system not only for cell biology, but also toxicology, drug screening, biogerontology and tissue engineering
TypeCell Biology
SpeciesHomo sapiens
Volume1.0 mL
Tissuekidney, Cortex:Epithelium:Proximal tubule
Cell LineRPTEC/TERT1
Tissue (standardized)Kidney
Cell Typeepithelial cell
Ageadult

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(41) Coordinated reprogramming of renal cancer transcriptome, metabolome and secretome associates with immune tumor infiltrationCancer cell internationalJanuary 5, 2023Poplawski P, Alseekh S, Jankowska U, Skupien-Rabian B, Iwanicka-Nowicka R, Kossowska H, Fogtman A, Rybicka B, Bogusławska J, Adamiok-Ostrowska A, et al.of cancer-associated fibroblasts (CAFs) in multiple cancer types. Methods Cell lines: RPTEC/TERT1 (CRL-4031), Caki-1 (HTB-46), 786-O (CRL-1932), A498 (HTB-44) cell lines were obtained from ATCC (American Type

(42) The complex, dynamic SpliceOme of the small GTPase transcripts altered by technique, sex, genetics, tissue specificity, and RNA base editingFrontiers in Cell and Developmental BiologyNovember 17, 2022Akansha S. Das, Emily C. Sherry, Robert M. Vaughan, Marian L. Henderson, Jacob Zieba, Katie L. Uhl, Olivia Koehn, Caleb P. Bupp, Surender Rajasekaran, Xiaopeng Li, et al.nanopore sequencing Renal proximal tubule epithelial cells immortalized with TERT1 (RPTEC-TERT1, ATCC, #CRL-4031) were grown under sheer stress and hypoxic conditions as previously described (Keele et al., 2021).

(43) Istradefylline protects from cisplatin-induced nephrotoxicity and peripheral neuropathy while preserving cisplatin antitumor effectsJ Clin InvestNovember 15, 2022Edmone Dewaeles, KÃ©vin Carvalho, Sandy Fellah, Jaewon Sim, Nihad Boukrout, Raphaelle Caillierez, Hariharan Ramakrishnan, Cynthia Van der Hauwaert, Jhenkruthi Vijaya Shankara, Nathalie Martin, et al.medullary region (72). Cell cultures. RPTECs immortalized with a pLXSN-hTERT1 retroviral vector (CRL-4031, ATCC) are a relevant in vitro model to evaluate cisplatin’s deleterious effects (73–75). Cells were

(44) Istradefylline protects from cisplatin-induced nephrotoxicity and peripheral neuropathy while preserving cisplatin antitumor effectsJ Clin InvestNovember 15, 2022Edmone Dewaeles, Kévin Carvalho, Sandy Fellah, Jaewon Sim, Nihad Boukrout, Raphaelle Caillierez, Hariharan Ramakrishnan, Cynthia Van der Hauwaert, Jhenkruthi Vijaya Shankara, Nathalie Martin, et al.medullary region (72). Cell cultures. RPTECs immortalized with a pLXSN-hTERT1 retroviral vector (CRL-4031, ATCC) are a relevant in vitro model to evaluate cisplatin’s deleterious effects (73–75). Cells were

(45) Multimodal single cell sequencing implicates chromatin accessibility and genetic background in diabetic kidney disease progressionNature communicationsSeptember 6, 2022Wilson PC, Muto Y, Wu H, Karihaloo A, Waikar SS, Humphreys BD.kit (Lonza; CC-3190). Human telomerase reverse transcriptase (hTERT)-immortalized human RPTEC (ATCC; CRL-4031) were cultured with ATCC hTERT Immortalized RPTEC Growth Kit (ATCC, ACS-4007). HEK293T cells (ATCC;

(46) 3D proximal tubule-on-chip model derived from kidney organoids with improved drug uptakeScientific ReportsSeptember 2, 2022Jeffrey O. Aceves, Szilvia Heja, Kenichi Kobayashi, Sanlin S. Robinson, Tomoya Miyoshi, Takuya Matsumoto, Olivier J. M. Schäffers, Ryuji Morizane, Jennifer A. Lewiscells/cm2. For the control chips, we obtained and cultured immortalized human PTECs (PTEC-TERT1s, ATCC CRL-4031) The culture protocol provided by the manufacturer was followed with one exception. We used a modified

(47) Phosphoproteomic Analysis of FLCN Inactivation Highlights Differential Kinase Pathways and Regulatory TFEB PhosphoserinesMolecular & cellular proteomics : MCPSeptember 1, 2022Glykofridis IE, Henneman AA, Balk JA, Goeij-de Haas R, Westland D, Piersma SR, Knol JC, Pham TV, Boekhout M, Zwartkruis FJT, et al.options. Experimental procedures Cell Culture Renal proximal tubular epithelial cells (RPTEC/TERT1, ATCC CRL-4031) were maintained in Dulbecco's modified Eagle's medium (DMEM)/F12 (Gibco, Life Technologies) according

(48) An isogenic cell line panel for sequence-based screening of targeted anticancer drugsiScienceJune 17, 2022Ashley L. Cook, Nicolas Wyhs, Surojit Sur, Blair Ptak, Maria Popoli, Laura Dobbyn, Tasos Papadopoulos, Chetan Bettegowda, Nickolas Papadopoulos, Bert Vogelstein, et al.#11875-119) supplemented with 10% fetal bovine serum (HyClone, Utah, USA, Cat #16777-006). RPTec cells (ATCC CRL-4031) are male in origin and were grown in EPITHELIAL CELL MEDIUM-Complete Kit (Science Cell Research, California,

(49) Exploring Noncovalent Protease Inhibitors for the Treatment of Severe Acute Respiratory Syndrome and Severe Acute Respiratory Syndrome-Like CoronavirusesACS Infectious DiseasesMarch 11, 2022Brendan T. Freitas et al.assessed in numerous diverse cell lines, including Renal Proximal Tubular Epithelial Cells (RPTECs, ATCC CRL-4031), BEAS-2B bronchial epithelial cells (ATCC CRL-9609), A549 human alveolar epithelial cells (ATCC, CCL-185),

(50) Analysis of human clinical and environmental Leptospira to elucidate the eco-epidemiology of leptospirosis in Yaeyama, subtropical JapanPLoS neglected tropical diseasesMarch 1, 2022Sato Y, Hermawan I, Kakita T, Okano S, Imai H, Nagai H, Kimura R, Yamashiro T, Kajita T, Toma C.performed using the software PAST version 4.06b [53]. Cell culture and infection RPTEC/TERT1 (ATCC CRL-4031) cells were grown in Dulbecco’s modified Eagle medium/nutrient mixture F-12 Ham (DMEM/F-12 Ham; Sigma-Aldrich)

(51) A single-domain i-body, AD-114, attenuates renal fibrosis through blockade of CXCR4JCI insightFebruary 22, 2022Cao Q, Huang C, Yi H, Gill AJ, Chou A, Foley M, Hosking CG, Lim KK, Triffon CF, Shi Y, et al.staining was quantified as percentage of total area, using ImageJ. Cell culture. RPTEC/TERT1 cells (ATCC CRL4031), a human PTC line that ectopically expresses the catalytic subunit of telomerase (TERT), were cultured

(52) Three dimensional modeling of biologically relevant fluid shear stress in human renal tubule cells mimics in vivo transcriptional profilesScientific ReportsDecember 1, 2021Emily J. Ross et al.kidney biology. Methods Maintenance of cell culture Human immortalized hRPTECs (RPTEC/TERT1, ATCC CRL-4031) and were cultured and maintained in hTERT Immortalized RPTEC Growth Kit (ATCC ACS-4007), supplemented

(53) CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cellsPLoS biologyDecember 1, 2021Bai Z, Feng J, Franken GAC, Al'Saadi N, Cai N, Yu AS, Lou L, Komiya Y, Hoenderop JGJ, de Baaij JHF, et al.cells were cultured in DMED/F12 medium supplemented with 5% of FBS. RPTEC/TERT1 cells were from ATCC (CRL-4031) and were grown according to the manufacturer’s instructions. CNNM3 and CNNM4 knockout cell lines The

(54) Cell spinpods are a simple inexpensive suspension culture device to deliver fluid shear stress to renal proximal tubular cellsScientific ReportsOctober 29, 2021Timothy G. Hammond, Corey Nislow, Ivan C. Christov, Vecihi Batuman, Pranay P. Nagrani, Marjan Barazandeh, Rohit Upadhyay, Guri Giaever, Patricia L. Allen, Michael Armbruster, et al.Endothelial cells immortalized with human Telomerase Reverse Transcriptase, RPTEC/TERT1 cells (ATCC CRL-4031, Manassas, VA, USA) as they show characteristic morphological and functional properties of PTC; e.g.

(55) Disassembly of the apical junctional complex during the transmigration of Leptospira interrogans across polarized renal proximal tubule epithelial cellsCellular microbiologySeptember 1, 2021Sebastián I, Okura N, Humbel BM, Xu J, Hermawan I, Matsuura C, Hall M, Takayama C, Yamashiro T, Nakamura S, et al.for 3 days with shaking at 30°C for cell infection experiments. 4.2 Cell culture RPTEC/TERT1 (ATCC® CRL‐4031™) cells were grown in Dulbecco's Modified Eagle Medium/Nutrient Mixture F‐12 Ham (DMEM/F‐12 Ham; Sigma‐Aldrich)

(56) Novel Dent disease 1 cellular models reveal biological processes underlying ClC-5 loss-of-functionHuman Molecular GeneticsJuly 9, 2021Mónica Durán, Carla Burballa, Gerard Cantero-Recasens, Cristian M Butnaru, Vivek Malhotra, Gema Ariceta, Eduard Sarró, Anna Meseguerproximal tubule epithelial cells RPTEC/TERT1 were obtained from the American Type Culture Collection (#CRL-4031, ATCC®). RPTEC/TERT1 were cultured in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (1:1,

(57) Sept8/SEPTIN8 involvement in cellular structure and kidney damage is identified by genetic mapping and a novel human tubule hypoxic modelScientific reportsJanuary 22, 2021Keele GR, Prokop JW, He H, Holl K, Littrell J, Deal AW, Kim Y, Kyle PB, Attipoe E, Johnson AC, et al.QTL. RPTEC-TERT1 hypoxia model Renal proximal tubule epithelial cells (RPTEC) immortalized with TERT1 (ATCC, CRL-4031) were grown to confluency in 6-well plates using DMEM:F12 supplemented with RPTEC growth kit (ATCC)

(58) Loss of FLCN-FNIP1/2 induces a non-canonical interferon response in human renal tubular epithelial cellseLifeJanuary 18, 2021Glykofridis IE, Knol JC, Balk JA, Westland D, Pham TV, Piersma SR, Lougheed SM, Derakhshan S, Veen P, Rooimans MA, et al.oncogenesis, we used an immortalized, diploid renal proximal tubular epithelial cell line (RPTEC/TERT1, ATCC CRL-4031; Wieser et al., 2008, hereafter called RPTEC) as a model system, which retains the capacity to form

(59) Annexin A1 Tripeptide Mimetic Increases Sirtuin-3 and Augments Mitochondrial Function to Limit Ischemic Kidney InjuryFrontiers in physiologyJanuary 1, 2021Suliman H, Ma Q, Zhang Z, Ren J, Morris BT, Crowley SD, Ulloa L, Privratsky JR.(Gibco). The human RPTEC line immortalized with pLXSN-hTERT retroviral transfection (RPTEC/TERT1– ATCC, CRL-4031) was kindly provided by Dr. Alex Grabner and cultured in the REGM Renal Epithelial Growth Medium Bullet

(60) Repeated dose multi-drug testing using a microfluidic chip-based coculture of human liver and kidney proximal tubules equivalentsScientific ReportsDecember 1, 2020Ni Lin, Xiaobing Zhou, Xingchao Geng, Christopher Drewell, Juliane Hübner, Zuogang Li, Yingli Zhang, Ming Xue, Uwe Marx, Bo LiGmbH. Human cell sources. The human HepaRG cell line and the human proximal tubule cell line RPTEC/TERT1 (CRL-4031) were purchased from ATCC (ATCC, Manassas, VA, USA). HHSteC cell line was purchased from ScienCell Research

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RPTEC/TERT1 from ATCC

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