RPTEC/TERT1 from ATCC

Product Specs
ItemRPTEC/TERT1
CompanyATCC
Price $8,670.00Supplier Page View Company Product Page
Catalog NumberCRL-4031
ApplicationsThese cells are proposed to be a valuable model system not only for cell biology, but also toxicology, drug screening, biogerontology and tissue engineering
TypeCell Biology
SpeciesHomo sapiens
Volume1.0 mL
Tissuekidney, Cortex:Epithelium:Proximal tubule
Cell LineRPTEC/TERT1
Tissue (standardized)Kidney
Cell Typeepithelial cell
Ageadult

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(21) Second Generation I-Body AD-214 Attenuates Unilateral Ureteral Obstruction (UUO)-Induced Kidney Fibrosis Through Inhibiting Leukocyte Infiltration and Macrophage MigrationInternational Journal of Molecular SciencesDecember 6, 2024Qinghua Cao, Michael Foley, Anthony J. Gill, Angela Chou, Xin-Ming Chen, Carol A. Pollocksupplied by AdAlta Limited (Melbourne, Australia). 4.2. Cell Culture RPTEC/TERT1 cell lines (ATCC® CRL4031™), a human proximal tubular cell line, ectopically express the catalytic subunit of telomerase (TERT).

(22) TET2 germline variants promote kidney disease by impairing DNA repair and activating cytosolic nucleotide sensorsNature CommunicationsNovember 7, 2024Xiujie Liang, Hongbo Liu, Hailong Hu, Eunji Ha, Jianfu Zhou, Amin Abedini, Andrea Sanchez-Navarro, Konstantin A. Klötzer, Katalin Susztakinsulin-transferrin-selenium (ITS), and 1% penicillin-streptomycin. Immortalized human RPTECs (ATCC; CRL-4031) were cultured in Dulbecco’s modified Eagle’s medium (DMEM)-F12, supplemented with hydrocortisone (25

(23) Evaluating nephrotoxicity reduction in a novel polymyxin B formulation: insights from a 3D kidney-on-a-chip modelAntimicrobial agents and chemotherapyOctober 8, 2024Payasi A, Yadav MK, Chaudhary S, Aggarwal A.procured from Hi-Media, Mumbai, India. Cell culture and cell seeding in the MPS RPTEC/hTERT1 (ATCC-CRL-4031) cells were obtained from Manassas, USA; cultured in DMEM:F12 medium. When cells reached 80%–90% confluency,

(24) Transiently formed nucleus-to-cilium microtubule arrays mediate senescence initiation in a KIFC3-dependent mannerNature CommunicationsSeptember 12, 2024Jielu Hao Robichaud, Yingyi Zhang, Chuan Chen, Kai He, Yan Huang, Xu Zhang, Xiaobo Sun, Xiaoyu Ma, Gary Hardiman, Ciaran G. Morrison, et al.dysregulation. Methods Cell culture, treatments, and transfections IMR90 (Cat# CCL-186), RCTE (Cat# CRL-4031), human embryonic kidney (HEK) 293 T cells (Cat# CRL-3216) were purchased from ATCC (American Type Culture

(25) A Cell Pre‐Wrapping Seeding Technique for Hydrogel‐Based Tubular Organ‐On‐A‐ChipAdvanced ScienceAugust 1, 2024Jing Nie, Sha Lou, Andreas M. A. O. Pollet, Manon van Vegchel, Carlijn V. C. Bouten, Jaap M. J. den Toonderwas stored at room temperature in vacuum until further use. Cell Culture RPTECs (RPTEC/TERT1, ATCC‐CRL‐4031, LGC Standards GmbH, Wesel, DE) were cultured in Dulbecco's modified Eagle's medium (DMEM): F12 medium

(26) An in vitro-in silico workflow for predicting renal clearance of PFASToxicology and Applied PharmacologyAugust 1, 2024Hsing-Chieh Lin, Courtney Sakolish, Haley L. Moyer, Paul L. Carmichael, Maria T. Baltazar, Stephen S. Ferguson, Jason P. Stanko, Philip Hewitt, Ivan Rusyn, Weihsueh A. Chiucompounds. 2.1.2. Cell culture and Exposure The human RPTEC TERT1-OAT1 was obtained from ATCC (Cat# CRL-4031-OAT1, Manassas, VA). Cells were cultured in DMEM:F12 (Cat# 30-2006, ATCC) supplemented with the hTERT

(27) Renal antiporter ClC-5 regulates collagen I/IV through the β-catenin pathway and lysosomal degradationLife science allianceJuly 1, 2024Durán M, Ariceta G, Semidey ME, Castells-Esteve C, Casal-Pardo A, Lu B, Meseguer A, Cantero-Recasens G.ultimately, halt Dent’s disease type 1 progression. Materials and Methods Cell culture RPTEC/TERT1 (#CRL-4031; ATCC) cells were grown in DMEM: Nutrient Mixture F-12 (1:1, vol/vol) (#31331093; Thermo Fisher Scientific)

(28) Transcriptomic, epigenomic, and spatial metabolomic cell profiling redefines regional human kidney anatomyCell metabolismMay 7, 2024Li H, Li D, Ledru N, Xuanyuan Q, Wu H, Asthana A, Byers LN, Tullius SG, Orlando G, Waikar SS, et al.in the kit. Primary RPTECs were used in early passage. hTERT immortalized RPTEC cells (RPTEC/TERT1) (CRL-4031, ATCC) were cultured in DMEM:F12 medium supplemented with 0.2% FBS (F4135, Sigma), 0.1 mg/mL G418 (10131035,

(29) Chromosome evolution screens recapitulate tissue-specific tumor aneuploidy patternsNature GeneticsMay 1, 2024Emma V. Watson, Jake June-Koo Lee, Doga C. Gulhan, Giorgio E. M. Melloni, Sergey V. Venev, Rayna Y. Magesh, Abdulrazak Frederick, Kunitoshi Chiba, Eric C. Wooten, Kamila Naxerova, et al.declare. Establishing clonal aneuploid cell lines The hTERT–RPTEC cell line was purchased from ATCC (CRL-4031), and the hTERT–HMEC cell line was immortalized previously in the Elledge lab from primary HMECs purchased

(30) Daily Caffeine Consumption May Increase the Risk of Acute Kidney Injury Related to Platinum-Salt Chemotherapy in Thoracic Cancer Patients: A Translational StudyNutrientsMarch 19, 2024Aghiles Hamroun, Antoine Decaestecker, Romain Larrue, Sandy Fellah, David Blum, Cynthia Van der Hauwaert, Arnaud Scherpereel, Alexis Cortot, Rémi Lenain, Mehdi Maanaoui, et al.RPTECs (renal proximal tubular epithelial cells) immortalized with a pLXSN-hTERT1 retroviral vector (CRL-4031, ATCC) are a relevant in vitro model to evaluate cisplatin’s deleterious effects [34,35,36]. Cells were

(31) Enhanced fatty acid oxidation through metformin and baicalin as therapy for COVID-19 and associated inflammatory states in lung and kidneyRedox BiologyDecember 1, 2023Verónica Miguel, Carlos Rey-Serra, Jessica Tituaña, Belén Sirera, Elena Alcalde-Estévez, J. Ignacio Herrero, Irene Ranz, Laura Fernández, Carolina Castillo, Lucía Sevilla, et al.Rockville, MD). Immortalized renal human proximal tubule epithelial cells RPTEC/TERT1 (HPTEC) (ATCC®; #CRL-4031) were cultured in DMEM/F12, GlutaMAX supplement (Dulbecco's modified Eagle's medium 1:1 (v/v)) (Life technologies

(32) Guanine quadruplexes mediate mitochondrial RNA polymerase pausingbioRxivOctober 22, 2023Ryan Snyder, Don Delker, Joshua T Burdick, Vivian G. Cheung, Jason A. Wattsmitochondrial polymerase pausing, hTERT-immortalized renal proximal tubule epithelial cells (RPTECs) (ATCC, #CRL-4031) maintained in serum free media were seeded on the bottom of collagen-coated permeable transwell membranes

(33) The essential role of the ERK activation in large T antigen of BK polyomavirus regulated cell migrationVirus researchOctober 15, 2023Wang JW, Li YJ, Wu HH, Hsu HH, Chang MY, Wang RY, Tian YC.methods 2.1 Cell culture Human renal proximal tubular epithelial cells (hRPTECs) cell line (ATCC® CRL-4031™) was cultured in DMEM/F-12 1:1 (Gibco™ 11330032) supplemented with 1% fetal bovine serum (Gibco™ 10437028)

(34) Reciprocal regulation between the molecular clock and kidney injuryLife science allianceOctober 1, 2023Rey-Serra C, Tituaña J, Lin T, Herrero JI, Miguel V, Barbas C, Meseguer A, Ramos R, Chaix A, Panda S, et al.epithelial cells RPTEC/TERT1 (HPTEC) were obtained from the American Type Culture Collection (#PCS-400-010 and #CRL-4031; ATCC, respectively). These cells were cultured in renal epithelial cell basal medium (#PCS-400-030;

(35) Degradation of p0071 and p120-catenin during adherens junction disassembly by Leptospira interrogansFrontiers in Cellular and Infection MicrobiologySeptember 15, 2023Romina Tokumon, Isabel Sebastián, Bruno M. Humbel, Nobuhiko Okura, Hidenori Yamanaka, Tetsu Yamashiro, Claudia Tomacell infection experiments. 2.2 Cell culture RPTEC/TERT1 (American Type Culture Collection, ATCC® CRL-4031™) cells were grown in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham (DMEM/F-12 Ham; Sigma-Aldrich,

(36) Renal cancer secretome induces migration of mesenchymal stromal cellsStem cell research & therapyAugust 10, 2023Popławski P, Zarychta-Wiśniewska W, Burdzińska A, Bogusławska J, Adamiok-Ostrowska A, Hanusek K, Rybicka B, Białas A, Kossowska H, Iwanicka-Nowicka R, et al.cancer cells. Material and methods Propagation of RCC cell lines and CM collection RPTEC/TERT1 (CRL-4031, ATCC), Caki-1 (HTB-46, ATCC), 786-O (CRL-1932, ATCC) and A498 (HTB-44, ATCC) were cultured in accordance

(37) 3D vascularised proximal tubules-on-a-multiplexed chip model for enhanced cell phenotypes†Lab on a ChipJuly 12, 2023Miguel Carracedo, Sanlin Robinson, Babak Alaei, Maryam Clausen, Ryan Hicks, Graham Belfield, Magnus Althage, Annette Bak, Jennifer A. Lewis, Pernille B. L. Hansen, et al.drug development and testing. Materials and methods Cell culture Human immortalized PTECs (RPTEC/TERT1; ATCC CRL4031) were cultured using the supplier's specified protocol with some modifications. The cell media was modified

(38) Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubuleCommunications BiologyMay 4, 2023Ramin Banan Sadeghian, Ryohei Ueno, Yuji Takata, Akihiko Kawakami, Cheng Ma, Toshikazu Araoka, Minoru Takasato, Ryuji Yokokawawere withdrawn on day 12. RPTEC/TERT1 culture Cryopreserved immortalized RPTEC/TERT1 cells (ATCC® CRL-4031™) stocked at 106 cell/vial with passage number less than 9 were thawed and subcultured in T25 cell culture

(39) Relationship between Adsorption and Toxicity of Nephrotoxic Drugs in Microphysiological Systems (MPS)MicromachinesMarch 29, 2023Ueno R, Kuninori M, Sumi T, Sadeghian RB, Takata Y, Iguchi A, Tsuda M, Yamashita F, Ichikawa K, Yokokawa R.temperature with 1 rpm rotation. After incubation, each medium was collected, and applied to the RPTECs (CRL-4031, ATCC, Manassas, VA, USA) cultured in 96-well plates (167008, ThermoFisher, Waltham, MA, USA). After

(40) PRDM10 directs FLCN expression in a novel disorder overlapping with Birt-Hogg-Dubé syndrome and familial lipomatosisHuman molecular geneticsMarch 20, 2023van de Beek I, Glykofridis IE, Oosterwijk JC, van den Akker PC, Diercks GFH, Bolling MC, Waisfisz Q, Mensenkamp AR, Balk JA, Zwart R, et al.F0804, Sigma-Aldrich, St. Louis, MO, USA). Renal proximal tubular epithelial cells (RPTEC/TERT1, ATCC CRL-4031™) were maintained in DMEM/F12 (Gibco, Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA,

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RPTEC/TERT1 from ATCC

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