Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human from Miltenyi Biotec

(1) Aging of hematopoietic stem cells is inconsequential to progenitor cell functionStem Cell ReportsJuly 14, 2026Jenna Myers, Atesh K. Worthington, Donna M. Poscablo, Paloma Medina, Saran Chattopadhyaya, Raana Mogharrab, Marcel G.E. Rommel, E. Camilla ForsbergBV605,BD Biosciences,745082 BD Calibrite-APC Beads,BD Biosciences,340487 CD117 – Miltenyi Beads,Miltenyi,130-091-2 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, Click-iT

(2) Clinical MAPPs: a personalized healthcare-driven assay for the direct identification of potential T cell epitopes in patientsFront ImmunolJuly 7, 2026Katharina Hartman, Guido Steiner, Cary M. Looney, Michel Siegel, Katharine Bray-French, Klaudia Brix, Sebastian Springer, Timothy P. Hickling, Niels Janssen, Axel Ducret, et al.was assessed by flow cytometry using the Dendritic Cell Enumeration Kit for Humans (Miltenyi: Cat: #130-091-086) with adaptations. In order to prevent unspecific binding of antibodies, Fc receptor (FcR) blocking

(3) ZIP8 loss impairs macrophage-mediated phagolysosomal removal of bacteria and is overcome by butyrate supplementationCommunications BiologyJanuary 12, 2026Deandra R. Smith, Sabah Haq, Meng Niu, Sunayana Malla, Rajib Saha, Ashley Peer, Piero Bianco, Svetlana Romanova, Derrick R. Samuelson, Daren L. KnoellBioLegend). Following fixation and wash, cells were resuspended in PB buffer (MACS Rinsing Solution cat# 130-091-022 &, MACS BSA solution cat# 130-091-376, Miltenyi Biotec) and stained for TFEB Alexa 647 (cat# ab2832744,

(4) Method for generation and ex vivo expansion of genetically edited mouse TregsJournal of Leukocyte BiologyDecember 1, 2025Yosef Gilad, Adam M Dean, Yan Xia, Sang Jun Han, David M Lonard, Bert W O'Malleysupernatant. The cells were then counted and subjected to Treg isolation using Miltenyi Biotec isolation kit (130-091-0410) according to the manufacturer's protocol. The cells were then plated on an anti-mouse CD3 antibody

(5) Microfluidics-free single-cell genomics reveals complex central-peripheral immune crosstalk in the mouse brain during peripheral inflammationResearch SquareMay 14, 2025Malú Tansey, Jake Boles, Oihane Uriarte Uriarte Huartebeads. Cells were suspended in 90μL of a buffer made of autoMACS Rinsing Solution (Miltenyi Biotec, #130-091-022) with 0.5% BSA (BB), to which 10μL CD45 MicroBeads (Miltenyi Biotec, #130-052-301) was added. A

(6) Exosomes from Tregs mitigate lung damage caused by smoking via inhibiting inflammation and altering T lymphocyte subsets in COPD ratsBMC Pulm MedApril 14, 2025Xuefang Tao, Hai Tian, Guowen Wang, Yongzhen Sun, Liangyan Zhaomagnetic-activated cell sorting separator (130-042-302, Miltenyi Biotec) and rinsed with 3 mL running buffer (130-091-211, Miltenyi Biotec) to obtain Tregs. Subsequently, flow cytometry successfully identified Tregs, followed

(7) Scm6A: A Fast and Low-cost Method for Quantifying m6A Modifications at the Single-cell LevelGenomics, Proteomics & BioinformaticsOctober 15, 2024Yueqi Li, Jingyi Li, Wenxing Li, Shuaiyi Liang, Wudi Wei, Jiemei Chu, Jingzhen Lai, Yao Lin, Hubin Chen, Jinming Su, et al.P1400, Solarbio). Subsequently, CD4+/CD8+ T cells were isolated from PBMCs using MACS beads (Catalog No. 130-091-101, Miltenyi Biotec, Bergisch Gladbach, Germany). Briefly, PBMCs were bound to CD4 microbeads (20 µl

(8) Differential proteins from EVs identification based on tandem mass tags analysis and effect of Treg-derived EVs on T-lymphocytes in COPD patientsRespiratory ResearchSeptember 28, 2024Xuefang Tao, Zhisong Xu, Hai Tian, Jingfeng He, Guowen Wang, Xuexia Taothe LS column in a MACS separator (130-042-302, Miltenyi Biotec) and rinse with 3 mL running buffer (130-091-211, Miltenyi Biotec); add the incubated cell suspension to the sorting column to collect unlabeled

(9) Peripheral endotoxin exposure in mice activates crosstalk between phagocytes in the brain and peripheryResearch SquareJune 8, 2024Jake Boles, Oihane Uriarte Uriarte Huarte, Malú Gámez Tanseybeads. Cells were suspended in 90μL of a buffer made of autoMACS Rinsing Solution (Miltenyi Biotec, #130-091-022) with 0.5% BSA (BB), to which 10μL CD45 MicroBeads (Miltenyi Biotec, #130-052-301) was added. Labeling

(10) Pan-PI3K inhibition with copanlisib overcomes Treg- and M2-TAM-mediated immune suppression and promotes anti-tumor immune responsesClinical and experimental medicineDecember 1, 2023Heller S, Glaeske S, Gluske K, Paul J, Böhme A, Janzer A, Roider HG, Montebaur A, Nicke B, Lesche R, et al.Buffer,GibcoBioLegend,14190144421401 MACS Buffer,19×1×,autoMACS Rising SolutionMACS BSA Stock Solution,Miltenyi BiotecMiltenyi Biotec,130-091-222130-091-376 MagCellect Buffer,9×1×,UltraPure Distilled WaterMagCellect Plus 10 × Buffer,InvitrogenR&D

(11) Alteration of Immunoregulatory Patterns and Survival Advantage of Key Cell Types in Food Allergic ChildrenCellsNovember 30, 2023Kamal Ivory, Rossella Angotti, Mario Messina, Denise Bonente, Ferdinando Paternostro, Massimo Gulisano, Claudio Nicolettidendritic cell enumeration kit purchased from Miltenyi Biotec Ltd. (Bergisch Gladbach, Germany, Cat. 130-091-086), which included CD141/BDCA-3-APC (myeloid cDC) and CD303/BDCA-2-FITC for plasmacytoid DC (PDC). Red blood cells

(12) Third-party cytomegalovirus-specific T cells improved survival in refractory cytomegalovirus viremia after hematopoietic transplantThe Journal of clinical investigationMay 15, 2023Prockop SE, Hasan A, Doubrovina E, Dahi PB, Rodriguez-Sanchez I, Curry M, Mauguen A, Papanicolaou GA, Su Y, Yao J, et al.CCR7 BV605 (catalog 353214) from BioLegend; and CD56 APC (catalog 130-113-30J) and IFN-γ FITC (catalog 130-091-091-641) from Miltenyi Biotec. We measured specific cytotoxic activity of CMVpp65-VSTs against CMVpp65

(13) Monitoring Blood Immune Cells in Patients with Advanced Small Cell Lung Cancer Undergoing a Combined Immune Checkpoint Inhibitor/ChemotherapyBiomoleculesJanuary 17, 2023Riemann D, Turzer S, Ganchev G, Schütte W, Seliger B, Möller M.Wiesbaden, Germany). DC subpopulations were identified with the “Blood DC Enumeration Kit” (Cat. No. 130-091-086; Miltenyi, Bergisch Gladbach, Germany) supplemented for gating reasons with CD45-APC-H7 and an HLA-DR-V500

(14) Light regulates stomatal development by modulating paracrine signaling from inner tissuesNature CommunicationsDecember 1, 2021Shenqi Wang, Zimin Zhou, Rini Rahiman, Grace Sheen Yee Lee, Yuan Kai Yeo, Xin Yang, On Sun Lau1610737) and immunoblotting was carried out with an HRP-conjugated anti-GFP antibody (Miltenyi Biotec, 130-091-83, 1:3000 dilution) or a Rabbit monoclonal anti-GFP antibody (Cell Signaling Technology, 2956, 1:1000

(15) Single-cell RNA sequencing reveals SARS-CoV-2 infection dynamics in lungs of African green monkeysScience Translational MedicineJanuary 27, 2021Emily Speranza, Brandi N. Williamson, Friederike Feldmann, Gail L. Sturdevant, Lizzette Pérez- Pérez, Kimberly Meade-White, Brian J. Smith, Jamie Lovaglio, Craig Martens, Vincent J. Munster, et al.and final washes in phosphate-buffered saline containing 0.1% MACS bovine serum albumin (Miltenyi, 130-091-386). A total of 10,000 cells were prepared for 10X Genomics gel bead emulsions. The 10X Genomics version

(16) A novel disorder involving dyshematopoiesis, inflammation, and HLH due to aberrant CDC42 functionThe Journal of experimental medicineDecember 2, 2019Lam MT, Coppola S, Krumbach OHF, Prencipe G, Insalaco A, Cifaldi C, Brigida I, Zara E, Scala S, Di Cesare S, et al.flow cytometric analysis Dendritic cell analysis was performed with the Blood Dendritic cell enumeration kit (#130-091-086; Miltenyi Biotec). This assay is based on dendritic cell–specific surface antigens CD303 (BDCA-2), CD141

(17) The Impact of Varying Cooling and Thawing Rates on the Quality of Cryopreserved Human Peripheral Blood T CellsScientific ReportsDecember 1, 2019Jasmin Baboo, Peter Kilbride, Mike Delahaye, Stuart Milne, Fernanda Fonseca, Magdalena Blanco, Julie Meneghel, Alex Nancekievill, Nick Gaddum, G. John Morrisanti-human CD3 PE Vio770 (#130-096-749), anti-human CD4 PE (#130-091-231) and anti-human CD8 APC Vio770 (#130-091-561) were obtained from Miltenyi Biotec (Cologne, Germany). The DMSO (#276855) was obtained from Sigma-Aldrich

(18) SHP1 and SHP2 inhibition enhances the pro-differentiative effect of phorbol esters: an alternative approach against acute myeloid leukemiaJournal of Experimental & Clinical Cancer ResearchDecember 1, 2019Alejandro Pérez-Fernández, Guillermo López-Ruano, Rodrigo Prieto-Bermejo, Carla Ijurko, María Díez-Campelo, Fermín Sánchez-Guijo, Ángel Hernández-HernándezCD11b-APC antibody (130-191-241) and HSC-CFU Methylcellulose medium basic (130-091-275) and complete w/o EPO (130–091-227) were from Miltenyi Biotec (Madrid, Spain). Polyethylenimine (MW 25000, #23966–1) was from Polysciences,

(19) AnnexinA5-pHrodo: a new molecular probe for measuring efferocytosisScientific ReportsDecember 7, 2018R. Stöhr, N. Deckers, L. Schurgers, N. Marx, C. P. Reutelingspergercentrifuged again labelled with CD11b according to the manufacturers instructions (Miltenyi Biotec 130-091-24) and re-suspended in 100 µL FACS buffer before analysis on a FACS Canto II (BD Bioscience). FACS

(20) A Reliable and Reproducible Model for Assessing the Effect of Different Concentrations of α-Solanine on Rat Bone Marrow Mesenchymal Stem CellsBone Marrow ResearchOctober 22, 2017Adriana Ordóñez-Vásquez, Lorenza Jaramillo-Gómez, Camilo Duran-Correa, Erandi Escamilla-García, Myriam Angélica De la Garza-Ramos, Fernando Suárez-Obando1 : 20 solution of MACS BSA Stock Solution (MACS Miltenyi Biotec, 130048-701) in MACS Buffer (AutoMACS, 130-091-22), then incubated with a secondary Anti-FITC antibody Microbeads (MACS Miltenyi Biotec, 130-048-701)

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Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human from Miltenyi Biotec

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Blood Dendritic Cell Phenotyping Cocktail Kit, anti-human from Miltenyi Biotec

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