Corning® Matrigel® Matrix for Organoid Culture, Phenol Red-free, LDEV-free, 10 mL from Corning Life Sciences

Product Specs
ItemCorning® Matrigel® Matrix for Organoid Culture, Phenol Red-free, LDEV-free, 10 mL
CompanyCorning Life Sciences
Price Supplier Page View Company Product Page
Catalog Number356255
Quantity1 / Pk
TypeVial
Source/Expression SystemEngelbreth-Holm-Swarm lathrytic mouse tumor
SourceEngelbreth-Holm-Swarm lathrytic mouse tumor
StorageAvoid multiple freeze-thaws. Do not store in frost-free freezer. KEEP FROZEN., Store at -20°C
Cell TypeOrganoid, Organoid Lines from Primary cells and Stem cells, Stem and progenitor cells
ApplicationsOrganoid Culture

Specifications/Features

Size: 10 mL

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Corning Life Sciences

836 North Street

Building 300 Suite 3401

Tewksbury, MA 01876

United States

Phone: (978) 442-2200
Phone: (800) 492-1110

Email: clscustserv@corning.com

Company Profile

Website: www.corning.com/lifesciences

(1) Profibrotic predictive toxicology in the lungFrontiers in PharmacologyFebruary 18, 2026Pooja Singh, Rajesh Sinha, Veena B. Antonycheck for cell density and viability. Cell suspension was mixed with corning organoid matrigel matrix (356255, Corning, United States) in 1:1 ratio. 8,000–10,000 cells (depending upon total number of cells and

(2) A plastic EMP1⁺ to LGR5⁺ cell state conversion as a bypass to KRAS-G12D pharmacological inhibition in metastatic colorectal cancerCancer DiscoveryFebruary 6, 2026Alessia Centonze, Adrià-Jaume Roura, Meritxell Novillo-Font, Cristina Giordano, Xavier Hernando-Momblona, Montserrat Llanses, Paula Prats, Marta Sevillano, Débora Cabot, Mireia Novell, et al.patient liver metastases. A total of 2 × 103 cells were plated in a 5 µL drop of Matrigel (Corning, 356255; cells diluted in basal media and mixed with Matrigel in a proportion 1:4) in a 96-well plate and cultured

(3) Decidualization-empowered ECM hydrogel integrating sustained Tβ4 release drives endometrial regeneration in intrauterine adhesionsNature CommunicationsJanuary 21, 2026Yuxiang Liang, Zhaowei Yu, Shaobo Du, Yuqian Guo, Jing Li, Yujia Yan, Shanshan Jin, Wenjing Liang, Mengyuan Li, Ning Jin, et al.Penicillin-Streptomycin Solution (cat. no. SV30010) from HyClone. Matrigel Basement Membrane Matrix (cat. no. 356255) was obtained from Corning. For assays and staining, Paraformaldehyde (PFA, cat. no. P1110), H&E Staining

(4) Lactate derived from cancer-associated fibroblasts promotes alternative splicing and castration resistance in prostate cancerScience AdvancesJanuary 16, 2026Diwei Zhao, Zijun Mo, Tianyou Zhang, Xinyang Cai, Zhenyu Yang, Dong Chen, Junliang Zhao, Yuanwei Li, Fangjian Zhou, Zhen Li, et al.(table S2). Briefly, the cell suspension containing 5000 cells was resuspended with 40 ml of Matrigel (356255, Corning), followed by plating onto a 24-well plate, as described previously (62). Human tumor tissues

(5) Development of potent, selective cPLA2 inhibitors for targeting neuroinflammation in Alzheimer’s disease and other neurodegenerative disordersnpj Drug DiscoveryJanuary 7, 2026Anastasiia V. Sadybekov, Marlon Vincent Duro, Shaowei Wang, Brandon Ebright, Dante Dikeman, Cristelle Hugo, Bilal Ersen Kerman, Qiu-Lan Ma, Antonina L. Nazarova, Arman A. Sadybekov, et al.astrocytes by seeding dissociated single cells at a density of 15,000 cells/cm2 on Matrigel (Corning, 356255) -coated plates and cultured in complete astrocyte medium (#1801, ScienCell). After 30 days of differentiation,

(6) Targeting CAMK1D-engineered nanoactivator suppresses cancer stem cell maintenance and immune evasion in enzalutamide-resistant prostate cancerTheranosticsJanuary 1, 2026Feifei Sun, Yuchuan Yan, Deqing Sun, Shijia Liu, Zhaoru Dong, Guoqiang Pan, Lin Zhang, Xianhao Shao, Yuliang Xu, Ying Qu, et al.prostatic cells were then embedded in 50 µl droplets of basement membrane extract (Corning; catalog no. 356255) and subsequently cultured in human prostate organoid medium (MasterAim, catalog no. 10-100-029). RNA sequencing

(7) CAMK1D activates AMPK/PINK1/Parkin-dependent mitophagy to promote enzalutamide resistance in prostate cancerCell Death & DiseaseDecember 19, 2025Feifei Sun, Ying Qu, Shuyu Mei, Lin Zhang, Xianhao Shao, Xinpei Wang, Shijia Liu, Meng Wang, Wenyao Liu, Muyi Yang, et al.(Biogenous, catalog no. B213152, Zhejiang, China), resuspended in cold Matrigel (Corning, catalog no. 356255, New York, USA), and plated as 40 µL domes in 24-well plates. After polymerization at 37 °C for 25 min,

(8) Derepression of the epithelial transcription factor GRHL2 promotes direct hepatocyte-to-cholangiocyte transdifferentiationPLoS BiolDecember 12, 2025Ludivine Vasseur, Céline Gheeraert, Julie Dubois-Chevalier, Ninon Very, Loïc Guille, Mohamed Bou Saleh, Clémence Boulet, Cyril Sobolewski, Pascal Loyer, Alexandre Berthier, et al.JetPrime as detailed hereabove were 48 h latter used for three-dimensional culture in Matrigel (Corning, 356255). 20,000 cells were resuspended in 5 µL of culture medium and gently mixed with 30 µL of Matrigel using

(9) Early life supplementation of Lactobacillus reuteri 21 promotes broiler intestinal proliferation and differentiation through indole-3-carboxaldehydePoultry ScienceDecember 1, 2025Yibin Xu, Binke Chen, Xiaoqing Ding, Bing Liu, Yongxia Wang, Aikun Fu, Xiuan Zhanstrainers to collect crypts between 45 and 150 μm. Finally, crypts were suspended in Matrigel (Cat: 356255, Corning), seeded in 24-well plates, and cultured in growth medium (Cat: 06010, IntestiCultTM, STEMCELL Technologies

(10) Isoallolithocholic acid ameliorates intestinal inflammation via metabolically reprogrammed macrophagesCommunications BiologyNovember 28, 2025Ying Wang, Weihui Yan, Hongxia Zhao, Ying Lu, Yahui Li, Shicheng Peng, Yongtao Xiaosingle cells. For the preparation of mouse organoids, 1000 crypts per well were embedded in Matrigel (#356255, Corning) within 24-well plates. The Matrigel was polymerized for 10 minutes at 37 °C and then immersed

(11) A human 3D culture-organ-on-chip platform for investigating the tumor microenvironment response to ionizing radiationiScienceNovember 26, 2025Jerome Lacombe, Sean E. Dunn, Marie Layac, Maria Soldevila, Nabhan M. Fakrudin, Brett Duane, Kurt Chen, Matthew W. Barrett, James Helton, Evagelia C. Laiakis, et al.on the experiment, in the central piece before being covered with 18 μL of cold Matrigel (Corning, 356255) to provide ECM-like microenvironment. After 10 min incubation at room temperature to allow Matrigel

(12) Establishment and transcriptomic characterization of canine organoids from multiple tissuesFrontiers in Cell and Developmental BiologyNovember 24, 2025Christopher Zdyrski, Vojtech Gabriel, Oscar Ospina, Hannah F. Nicholson, Michael Catucci, Bryan J. Melvin, Hannah Wickham, Dipak Kumar Sahoo, Kimberly Dao, Leeann S. Aguilar Meza, et al.listed in (Supplementary File S2) along with the usage of Matrigel Matrix for Organoid Culture (Corning; 356255). Total media volumes typically consisted of 500 µL on Monday and Wednesday, and 750 µL on Friday. Passaging

(13) JNK knockdown enhances CAR-T cell cytotoxicity through elevated NFATc1-dependent transcription in preclinical ovarian cancer modelsJournal for ImmunoTherapy of CancerNovember 18, 2025Charles Joseph Kuhlmann, Chloe E Jepson, Madison T Blucas, Fatema M Suleiman, Anusha Manda, Yoshiko N Kamata, Masakazu KamatamCherry+-OVCAR8, firefly luciferase+-OVCAR8 cells were injected subcutaneously in 50% Matrigel (Corning #356255). After 3 days, 1.5×10⁶ CAR+ T cells were injected intravenously. Tumor size was measured by calipers.

(14) Protocol to Establish Estrogen Receptor-Negative Heterozygous BRCA1 OrganoidsMethods and ProtocolsNovember 1, 2025Madhura Deshpande, Jeannine Gerhardtgrowth factor reduced (Corning, Cat # 356231, Charlotte, NC, USA)Matrigel, organoid (Corning, Cat # 356255, Charlotte, NC, USA)1 M magnesium chloride (MgCl2 Sigma Aldrich, Cat #M1028, St Louis, MI, USA)Paraformaldehyde

(15) High-fat diet driven post-operative colon cancer recurrence is dependent upon genetic susceptibility to deoxycholic acidCancer LettersOctober 28, 2025Ryan Morgan, Mohammad Amin Bayat Bayat Tork, Zitong Lin, Claire Wild, Luke R. Frietze, Sihao Huang, Meejeon Roh, Andrea Olivas, Colin W. Steele, Tao Pan, et al.and 0.9% penicillin/streptomycin by volume. Organoids were grown in Matrigel scaffolding (Corning, 356255) with a 2:1 ratio of Matrigel to organoids suspended in complete media. 50 μL dots of Matrigel/Organoid

(16) Ubiquitin-independent pathway regulates the RIT1-MAPK pathway in chordoma progressionCell Death DisOctober 24, 2025Hui Chen, Qiujing Guan, Cheng Yang, Yang Chen, Yan Liu, Wenjie Ren, Su Chen, Lei Li, Dongxia Li, Jianguo Tang, et al.10992-1-AP, Proteintech), anti- Brachyury antibody (catalog number 81694, CST), Matrigel (catalog number 356255, Corning), red blood cell lysis buffer (catalog number P0013D, Beyotime), RIPA lysis buffer (catalog

(17) A Plastic EMP1+ to LGR5+ Cell State Conversion as a Bypass to KRASG12D Pharmacologic Inhibition in Metastatic Colorectal CancerCancer DiscovOctober 21, 2025Alessia Centonze, Adrià-Jaume Roura, Meritxell Novillo-Font, Cristina Giordano, Xavier Hernando-Momblona, Montserrat Llanses, Paula Prats, Marta Sevillano, Débora Cabot, Mireia Novell, et al.patient liver metastases. A total of 2 × 103 cells were plated in a 5 µL drop of Matrigel (Corning, 356255; cells diluted in basal media and mixed with Matrigel in a proportion 1:4) in a 96-well plate and cultured

(18) Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluidbioRxivOctober 16, 2025Taryn B. Gellner, Belinda Chen, Shreyas R. Raini, Mackenzie S. Jackson, Amy K. Kraak, Sophie C. Petta, Margherita Paschini, Lynn M. Schnapp, Carla F. Kim, Monica Yun Liuuse within two freeze-thaw cycles. We use Matrigel that is optimized for organoid culture (Corning 356255). Once thawed, we store Matrigel at 4°C and use within 2 weeks. To develop these protocols, we used

(19) A Practical Guide to Developing and Troubleshooting Patient-Derived "Mini-Gut" Colorectal Organoids for Clinical ResearchMethods ProtocOctober 11, 2025Rex Devasahayam Arokia Balaya, Zahra Heydari, Gobinda Sarkar, Estela Mariel Cruz Garcia, Jose M. de Hoyos-Vega, Eugene Krueger, Lauren Helgeson, Alexander Revzin, Alexandra Ros, Akhilesh Pandey, et al.were performed using Corning Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix (Cat. No. 356255). Each lot was accompanied by a certificate of analysis indicating a protein concentration in the range

(20) Immunometabolic defects of CD8+ T cells disrupt gut barrier integrity in people with HIVCellOctober 2, 2025Upasana Das Adhikari, Leah M. Froehle, Alexandra N. Pipkin, Heeva Baharlou, Alice H. Linder, Palak Shah, Amanda Hussey, Qiming Zhang, Sarah Nyquist, Saleh Khawaled, et al.H3375 L-glutamine,Sigma Aldrich,Cat# 25030–081 RPMI Medium,Thermo Fisher Scientific,Cat# 21870092 Matrigel,Corning,Cat# 356255 TrypLE™ Express Enzyme,Thermo Fisher Scientific,Cat# 12604013 L-WRN Conditioned Media,This paper,n/a Advanced DMEM/F-12

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