NucleoCounter® NC-200™ Automated Cell Counter from ChemoMetec A/S

Product Specs
ItemNucleoCounter® NC-200™ Automated Cell Counter
CompanyChemoMetec A/S
Price Get Pricing Get Quote for this Product
Catalog NumberNC-200
QuantityEA
ApplicationsCell count & viability for mammalian cells
Cell Size Range5×10⁴ to 5×10⁶ cells/ml (for counting)
Dimensions(WxDxH) 40 x 25 x 23 cm
Cell TypeMammalian cells, including cell lines, leukopaks, PBMCs, whole blood, MSCs, T-cells, aggregated cells, & microcarriers
Analysis Time50 seconds (one step), 120 seconds (two steps)
VolumeAnalysis volume: 1.4 µl
Loading volume: 60 µl
OpticsLens with x1.3 magnification, 1/2.” CMOS camera
EmissionA single dual-band emission filter: 410 to 460 nm and 540 to 650 nm
Excitation Wavelength2 LED light sources peaking at 365 nm and 505 nm, dark field light

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ChemoMetec A/S

ChemoMetec Inc.

3920 Veterans Memorial Highway- Suite 3

Bohemia, NY 11716

United States

Phone: Phone: 888 988 8542
Fax: 631 676 4236

Email: contact@chemometec.com
sales@chemometec.com

Company Profile

Website: http://www.chemometec.com

(1) Assessing MAPPs assay as a tool to predict the immunogenicity potential of protein therapeuticsLife Science AllianceJanuary 1, 2024Andrea Di Ianni, Tiziana Fraone, Piercesare Balestra, Kyra Cowan, Federico Riccardi Sirtori, Luca Barberophase contrast (Thermo Fisher Scientific). Cell recovery and viability were analyzed by NucleoCounter NC200 automated cell counter (ChemoMetec). ImMo-DCs and mMo-DCs were labeled with fluorochrome-conjugated

(2) CRISPR-Cas12a-integrated transgenes in genomic safe harbors retain high expression in human hematopoietic iPSC-derived lineages and primary cellsiScienceDecember 15, 2023Arsenios Vlassis, Tanja L. Jensen, Marina Mohr, Dominika J. Jedrzejczyk, Xiangyou Meng, Gergo Kovacs, Martí Morera-Gómez, Andrea Barghetti, Sergi Muyo Abad, Roland F. Baumgartner, et al.medium with 10 μM Y-27632. Viability and cell counts were measured using Via1 cassettes on Nucleocounter-NC200 (Chemometec). iPSC viability before EP was >70% in all experiments. For iPSC electroporation, the P3 Primary Cell 96-well Nucleofector Kit

(3) Analysis of the effects of bench-scale cell culture platforms and inoculum cell concentrations on PSC aggregate formation and cultureFront Bioeng BiotechnolDecember 1, 2023Diepiriye G. Iworima, Robert K. Baker, James M. Piret, Timothy J. KiefferY27632. Duplicate aliquots were collected for cell counts and viability using the Nucleocounter-200® (NC-200™; Chemometec), and the cells were reseeded at the appropriate densities. For a 3-, 4- or 5-day schedule,

(4) Cytokines impact natural killer cell phenotype and functionality against glioblastoma in vitroFrontiers in ImmunologySeptember 28, 2023Minna Sivonen, Katja A. Sirviö, Sara Wojciechowski, Anssi Kailaanmäki, Satu Kaipainen, Aubrey Bailey, Martin Villalba, Tuija Kekarainenkept approximately at 1x106 cells/ml during expansion. Cell count and viability were determined using NucleoCounter® NC 200™ automated cell counter (ChemoMetec), or Guava MUSE® cell analyzer using Muse Count & Viability Kit (Luminex). Used activation

(5) Development of biomass waste-based carbon quantum dots and their potential application as non-toxic bioimaging agents†RSC AdvancesSeptember 18, 2023Norhidayah Abu, Shanmugavel Chinnathambi, Mahima Kumar, Fatemeh Etezadi, Noremylia Mohd Bakhori, Zuhana Ahmad Zubir, Shahrul Nizam Md Salleh, Rafidah Hanim Shueb, Subramani Karthikeyan, Vaijayanthi Thangavel, et al.70% confluency. To determine cellular proliferation and viability, we analyzed the hiPSCs using the NucleoCounter NC-200 automated cell counter with Via 1-Cassette from ChemoMetec in Copenhagen, Denmark. We also visually inspected the adhesion

(6) Non-ammoniagenic proliferation and differentiation media for cultivated adipose tissueFrontiers in Bioengineering and BiotechnologyJuly 24, 2023S. Hubalek, J. Melke, P. Pawlica, M. J. Post, P. Moutsatsouperformed. Cell counts were always performed on at least two independent samples with the use of Nucleocounter (NC200™, Chemometec). Briefly, a homogeneous cell/microcarrier suspension was lysed with reagent A100 (910–0003,

(7) Fendrr synergizes with Wnt signalling to regulate fibrosis related genes during lung development via its RNA:dsDNA triplex elementNucleic Acids ResearchJuly 7, 2023Tamer Ali, Sandra Rogala, Nina M Krause, Jasleen Kaur Bains, Maria-Theodora Melissari, Sandra Währisch, Harald Schwalbe, Bernhard G Herrmann, Phillip Grotecentrifugation at 1000 rpm for 4 min. The pellet was resuspended in fresh medium and counted using a Chemometec NucleoCounter NC-200 Automated Cell Counter (Wotol #2194080-18). 0.15 × 106 cells were seeded per well (Greiner Bio-One™ #657160). Generation of

(8) Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxivJune 19, 2023Andrew J. Stout, Xiaoli Zhang, Sophia M. Letcher, Miriam L. Rittenberg, Michelle Shub, Kristin M. Chai, Maya Kaul, David L. Kaplanmaximum of 70% confluence, harvested using 0.25% trypsin-EDTA (ThermoFisher #25200056), counted using an NC-200 automated cell counter (Chemometec, Allerod, Denmark) and either seeded at a density of 2,000–5,000

(9) Acceleration of burn wound healing by micronized amniotic membrane seeded with umbilical cord-derived mesenchymal stem cellsMaterials Today BioJune 7, 2023Zixuan Zhou, Jingnan Xun, Chenghao Wu, Chao Ji, Shizhao Ji, Futing Shu, Yuxiang Wang, Hao Chen, Yongjun Zheng, Shichu Xiaogroups, respectively. The number of cells in the mAM-MSC group was calculated using a NucleoConuter NC-200 (ChemoMetec, Denmark) [44,45], and the cell number in mAM-MSC was adjusted to 5 × 105. In the MSC

(10) Generation of left ventricle-like cardiomyocytes with improved structural, functional, and metabolic maturity from human pluripotent stem cellsCell Rep MethodsApril 24, 2023Dark N, Cosson MV, Tsansizi LI, Owen TJ, Ferraro E, Francis AJ, Tsai S, Bouissou C, Weston A, Collinson L, et al.(Tocris) and grown until they reached 70–80% confluency. Cell seeding density was determined using the NC200 cell counter (Chemometec, Denmark). Seeding density was optimized for each cell line; accuracy in seeding

(11) Aggregating in vitro-grown adipocytes to produce macroscale cell-cultured fat tissue with tunable lipid compositions for food applicationseLifeApril 4, 2023John Se Kit Yuen Jr, Michael K Saad, Ning Xiang, Brigid M Barrick, Hailey DiCindio, Chunmei Li, Sabrina W Zhang, Miriam Rittenberg, Emily T Lew, Kevin Lin Zhang, et al.drug",Primocin,InvivoGen,ant-pm-1, Other,Accumax,Innovative Cell Technologies,AM105,Cell detachment reagent Other,NucleoCounter,Chemometec,NC-200,Cell counter "Peptide, recombinant protein",Insulin,Sigma-Aldrich,I0516, "Chemical compound, drug",Dexamethasone,Sigma-Aldrich,D4902, "Chemical

(12) Continuous fish muscle cell line with capacity for myogenic and adipogenic-like phenotypesScientific ReportsMarch 29, 2023Michael K. Saad, John S. K. Yuen, Connor M. Joyce, Xinxin Li, Taehwan Lim, Talia L. Wolfson, Justin Wu, Jason Laird, Sanjana Vissapragada, Olivia P. Calkins, et al.and 10 µg/mL gentamicin (Sigma Aldrich #G1397). The cells in growth medium were then counted using a NucleoCounter NC-200™ automated cell counter (Chemometec, Allerod, Denmark) and seeded at ~ 100,000 cells/cm2 into tissue culture-treated flasks.

(13) Ploidy-stratified single cardiomyocyte transcriptomics map Zinc Finger E-Box Binding Homeobox 1 to underly cardiomyocyte proliferation before birthBasic research in cardiologyMarch 2, 2023Bak ST, Harvald EB, Ellman DG, Mathiesen SB, Chen T, Fang S, Andersen KS, Fenger CD, Burton M, Thomassen M, et al.Dissociation Kit 130–098-373) according to the manufacturer’s recommendations. Following viable cell counting (NC-200, ChemoMetec), dissociated cells were stained with a fixable viability stain (Fixable viability stain 570

(14) Characterization of human induced pluripotent stems cells: Current approaches, challenges, and future solutionsBiotechnology ReportsMarch 1, 2023Sahana Suresh Babu, Haritha Duvvuru, Jillian Baker, Stephanie Switalski, Mehdi Shafa, Krishna Morgan Panchalingam, Saedeh Dadgar, Justin Beller, Behnam Ahmadian Baghbaderaniprecision, accuracy and linearity with respect to the hemocytometer. For iPSC, we have shown that NC-100 and NC-200 automated cell counting devices can be used for the estimation of viable cells and viability of cells

(15) In Vitro Evaluation of Antiprotozoal Properties, Cytotoxicity Effect and Anticancer Activity of New Essential-Oil Based Phytoncide MixturesMoleculesFebruary 1, 2023Hubert Iwiński, Henryk Różański, Natalia Pachura, Aleksandra Wojciechowska, Tomasz Gębarowski, Antoni Szumnycontaining acridine orange (AO) and 4′,6-diamidino-2-phenylindole (DAPI). The cells were counted in line NC200 (Chemometek, Allerod, Denmark) and the final cell count after inoculation in a 96-well plate was approximately

(16) Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meteriScienceJanuary 20, 2023Christoph Drobek, Juliane Meyer, Robert Mau, Anne Wolff, Kirsten Peters, Hermann Seitzwas dried with an air stream. Both cell number and cell diameter distribution were measured with the NC-200 and the Via1 cassette (both ChemoMetec A/S, Denmark). The cell number, the standard output of this device,

(17) Machine learning and metabolic modelling assisted implementation of a novel process analytical technology in cell and gene therapy manufacturingScientific ReportsJanuary 16, 2023Thomas Williams, Kevin Kalinka, Rui Sanches, Greg Blanchard-Emmerson, Samuel Watts, Lee Davies, Carol Knevelman, Laura McCloskey, Peter Jones, Kyriacos Mitrophanous, et al.determine viable cell concentration (VCC) and cell viability via analysis on an automated NucleoCounter NC200 (ChemoMetec, Denmark). Online bioreactor process parameters were recorded every minute using a supervisory

(18) Efficient derivation and banking of clinical-grade human embryonic stem cell lines in accordance with Japanese regulationsRegen TherNovember 6, 2022Kei Takada, Ryoko Nakatani, Emiko Moribe, Shizuka Yamazaki-Fujigaki, Mai Fujii, Masayo Furuta, Hirofumi Suemori, Eihachiro Kawaseconfirmation,,Undifferentiated: >80% of colonies,○,○,○ Cell count,Cell count,NucleoCounter (ChemoMetec)NC-200/Via-1 Cassette (ChemoMetec),,○,○,○ Viability,Acridine Orange/DAPI staining,NucleoCounter (ChemoMetec)NC-200/Via-1

(19) Impact of SARS-CoV-2 RBD Mutations on the Production of a Recombinant RBD Fusion Protein in Mammalian CellsBiomoleculesAugust 24, 2022Guillaume Gerez, Jerome Martinez, Christophe Steinbrugger, Sandra Bouanich, Johanna Dimino, Corine Piegay, Maxime Combe, Franck Berthier, Soizic DanielHEK-293 cell line [57] that has been adapted to suspension growth (293F). Cell number was determined using a NucleoCounter NC-200 automated cell counter (ChemoMetec, Allerod, Denmark; 900-0200) and the Via1-Cassette (ChemoMetec, Allerod, Denmark; 941-0012). For

(20) Improved Time Resolved KPI and Strain Characterization of Multiple Hosts in Shake Flasks Using Advanced Online Analytics and Data ScienceBioengineering (Basel)July 25, 2022Rüdiger W. Maschke, Barbara Pretzner, Gernot T. John, Christoph Herwig, Dieter Eiblusing a Cedex HiRes (Roche Custom Biotech) for the CHO and High Five cells, and with a NucleoCounter NC200 (Chemometec A/S, Allerod, Denmark) for the HEK293 cells. Metabolites (glucose, glutamine, ammonia),

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(1)

4/3/2015An easy to use cell counter with accurate results Yibing WangWe are working with some tough tissue digestion samples, such as skin, and need a way to obtain accurate live cell counts efficiently for the following applications. The manual counting method using ... Read Review
(1)

1/22/2014Fast, Easy and Reliable Cell Counting Anders LassenOur group mainly use the NC-200 for viability checks and cell counting in primary cultures. All is done in one step. Due to FACS-style gating we also get a _rough_ estimate of the culture composition... Read Review