BD FACSymphony™ A1 Cell Analyzer from BD Biosciences

Product Specs
ItemBD FACSymphony™ A1 Cell Analyzer
CompanyBD Biosciences
Price Get Pricing Get Quote for this Product
Catalog NumberFACSymphony A1
QuantityEA
DetectionFSC, SSC, Small Particle SSC, and 16 fluorescence detection channels.
Absorbance / Excitation405 nm, 488 nm, 561 nm, 637 nm
CapacityTubes (5 mL), Microplates using autosampler (96-well, 384-well)
Sample Type12 x 75-mm tubes; Optional High-Throughput Sampler for 96- or 384-well microtiter plate.
Excitation Wavelength405 nm (100 mW), 488 nm (100 mW), 561 nm (100 mW), 637 nm (100 mW)
Measuring Range16 fluorescence channels
Throughput25,000 events per second
Data Analysis10,000 events/sec; 25,000 events/sec (max)
Flow Rate12 to 60 µL/min (continuously adjustable)
Dimensions(WxDxH) 58 x 61 x 59 cm (23 x 24 x 23 in.)
TypeCell Analyzer
DetectorTube PMT
SoftwareBD FACSDiva™ v9.0.2 Software or later
Lasers4 lasers

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BD Biosciences

155 North McCarthy Blvd

Milpitas, California 95035

United States

Company Profile

Website: www.bdbiosciences.com

(1) Human and mouse regenerative macrophages enhance beta cell survival, function, and proliferationIsletsDecember 31, 2026Mahdis Monajemi, Alexandra S. Craciun, Qing Huang, Lei Dai, Majid Mojibian, Sarah Q. Crome, C. Bruce Verchere, Megan K. Levingsadded, incubated for 30 min, followed by another wash. The plate was read directly on a flow cytometer FACSymphony A1 (BD Biosciences), and data analysis was performed using the LEGENDplex™ cloud-based software. Flow cytometry

(2) Protocol for assessing anti-Siglec-9 antibody-mediated tumor phagocytosis by macrophages using pH-sensitive dyes and flow cytometrySTAR ProtocMay 20, 2026Saitong Muneekaew, Thunchanok Nuchphongsai, Pasut Sasithong, Kritayaporn Saiprayong, Kamonphan Rungrueang, Methichit WattanapanitchNo. 130042303 MiniMACS™ Separator,Miltenyi Biotec,Cat. No. 130042102 Flow cytometer,BD Biosciences,FACSymphony A1™ ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, K562 cells

(3) Engineering functionality-optimized fully human B7-H3 CAR T cells for enhanced solid tumor therapyCell Rep MedMay 19, 2026Pradip Bajgain, Yang Feng, Mariela Puebla, Meijie Tian, Kuo-Sheng Hsu, Jaewon Lee, GuoJun Yu, Liping Yang, Steven Seaman, Mary Beth Hilton, et al.(Jackson ImmunoResearch, cat no. 109-606-097 and 109-116-097). Fluorescence signals were acquired using a FACSymphony A1 Cell Analyzer (BD Biosciences) and flow cytometry data were analyzed using FlowJo version 10.9.0 or

(4) Intracellular Activation of Dual-Pharmacophore Artezomib Enhances Selective Cytotoxicity in Hematologic MalignanciesJ Med ChemMay 14, 2026Daqiang Li, Xiangao Huang, Zoe Chow, Jianxiang Ye, Renee Ma, Michael Zhu, David Jayabalan, Hao Zhang, Pengbo Zhou, Selina Chen-Kiang, et al.Samples were incubated for 30 min at 37 °C in the dark. Cell cycle distribution was analyzed using a FACSymphony A1 Flow Cytometer (BD Biosciences). ROS Detection Assay Intracellular reactive oxygen species (ROS) levels

(5) A functional crosstalk between circulating follicular helper 2 T cells and memory B cells drives anti-Plasmodium vivax antibodiesPLoS Negl Trop DisApril 24, 2026Zulfa Zahra Salsabila, Piyawan Kochayoo, Tipanan Khunsri, Pachara Tianpothong, Chaniya Leepiyasakulchai, Pongsakorn Thawornpan, Julius Clemence R. Hafalla, John H. Adams, Patchanee ChootongPD-1, CXCR3, CCR6, ICOS, CD45RA, CD40 ligand (CD40L), TIGIT, IL-4, and IL-10. Analyses were done with a FACSymphony A1 (BD Bioscience, USA); FACS data were examined using FlowJo software version 10.10.0 and presented as

(6) Global stabilization of the transcriptome in mitotic cellsThe EMBO JournalApril 9, 2026Ekaterina Khalizeva, Arash Latifkar, Kehui Xiang, David P Bartel, Jimmy Ly, Iain M Cheesemanmin before being strained into flow cytometry tubes. Analysis of cell cycle stage was done using the BD FACSymphony A1 Cell Analyzer (BD Biosciences) and quantified using FlowJo v10.9.0. We first gated cells using forward scatter and

(7) Flow Cytometry Role in Unlocking New Frontiers for Nanomedicine Applications of Plant‐Derived VesiclesJournal of Extracellular BiologyMarch 16, 2026Tamer Esmail, Sabino Porro, Francesca D'Ascanio, Domenico De Bellis, Arianna Aquilini‐Mummolo, Giulia Colasante, Pasquale Simeone, Ayesha Younas, Marwa Balaha, Mariagiulia Filoso, et al.synthetic nanoparticles in the range 7–1000 nm at the single‐particle level (Choi et al. 2019). The FACSymphony A1 Cell Analyzer (Becton Dickinson) can be equipped with a Small Particle Detector, an optional side scatter

(8) A beneficial environment promotes immune resilience through epigenetic regulationScience AdvancesFebruary 27, 2026Guilherme Dragunas, Markus Klotz, Sirui Chen, Zeynep Ertüz, Xiaomei Tan, Ülkü Rabia Korkmaz, Soni Shankhwar, Bettina Rankl, Deepesh Dhakad, Jimmy Omony, et al.and subsequently stained with intracellular markers (table S1). The samples were analyzed using the BD FACSymphony A1 Cell Analyzer running BD FACSDiva v8. Flow cytometry data were analyzed using FlowJo v10. Western blot Protein was

(9) Synergistic Effects of Nutritional Formula on Joint Inflammation Through Modulation of Bone Metabolism in RatsNutrientsFebruary 26, 2026Haitao Wang, Yi Wang, Dancai Fan, Zhenhua Niu, Hongming Su, Ang Li, Ruixin Kou, Ziyi Yue, Sihao Wu, Huan Lv, et al.µL of propidium iodide (PI) for 15 min. The cell samples were then analyzed using a flow cytometer (FACSymphony A1, BD Biosciences, San Jose, CA, USA), and the acquired data were processed using FlowJo software (v10.10,

(10) A toolkit for programmable transcriptional engineering across eukaryotic kingdomsbioRxivFebruary 16, 2026Izaiah J. Ornelas, Lauren A. Owens, Simon Alamos, Niklas F.C. Hummel, Mitzi G. Hernández Zamora, Ngan T. Phan, Rithu K. Pattali, Patrick M. Shih, James K. Nuñezroom temperature, washed twice with PBS containing 10% FBS, and protein expression was measured on a BD FACSymphony A1 Cell Analyzer (BD Biosciences) flow cytometer. All flow cytometry data generated was analyzed using FlowJo. Essential

(11) In vitro characterization and angiogenic potential of quality-and-quantity mononuclear cell–derived extracellular vesiclesJVS-Vascular ScienceFebruary 12, 2026Suttipong Tianwattanatada, Nuttapol Chruewkamlow, Narinee Srimark, Ladawan Khowawisetsut, Kovit Pattanapanyasat, Chutipon Sathan-ard, Pisol Senawongse, Nuttawut SermsathanasawadiBiosciences), and Annexin V FITC Detection Kit (#556547; BD Biosciences). EVs were evaluated using a BD FACSymphony A1 Cell Analyzer (BD Biosciences). Western blot analysis The intracellular protein marker calnexin was assessed. Proteins

(12) Recombinant ROP8 antigen: diagnostics, immunogenicity, and therapeutic targeting in toxoplasmosisFrontiers in ImmunologyFebruary 5, 2026Maciej Chyb, Adrian Bekier, Malwina Kawka, Bartłomiej Ferra, Bożena Dziadek, Sylwia Michlewska, Katarzyna Dzitko, Nazar Trotsko, Justyna GatkowskaFaculty of Biology and Environmental Protection, University of Lodz. Data acquisition was performed on FACSymphony A1 (BD Biosciences, San Jose, CA, USA) flow cytometer, which is calibrated daily, with 405 nm laser and

(13) Targeting DHODH reveals a metabolic vulnerability in AR-positive and AR-negative prostate cancer cells via pyrimidine synthesis and metabolic crosstalk with the TCA and urea cyclesMolecular MetabolismFebruary 1, 2026Maxime Labroy, Marc-Oliver Paré, Line Berthiaume, Mélissa Thomas, Cynthia Jobin, Alain Veilleux, Martin Pelletier, Frédéric Pouliot, Jean-Yves Masson, Étienne Audet-Walshand unstained tubes were prepared. Tubes were kept at 4 °C on ice overnight before analysis using a FACSymphony A1 (BD Biosciences) at the flow cytometry facility of the Centre de recherche du CHU de Québec – Université

(14) Systematic evaluation of tools for auxin-inducible protein degradation in budding yeastMolecular Biology of the CellFebruary 1, 2026Petra Hubbe, Charu Sharma, Oliver Pajonk, Niklas Peters, Nadja Guschtschin-Schmidt, Natalie Friemel, Sebastian Schuckindicated. Total cell fluorescence after excitation with a 488 nm laser was measured with a FACSCanto or a FACSymphony A1 flow cytometer (BD Biosciences), and the geometric mean of the cell population was calculated with FlowJo

(15) PD-1 suppresses CAR signaling by forming the inhibitory signalosome colocalizing to CAR microclustersCommunications BiologyJanuary 8, 2026Yosuke Yoshida, Hiroaki Machiyama, Ei Wakamatsu, Kensho Saito, Arata Takeuchi, Tetsushi Nishikawa, Ryohei Matsushima, Wataru Nishi, Hitoshi Nishijima, Yoshihiko Kanno, et al.concentration of 2.5 μg/mL. A cell sorter, SH800 (Sony), was used for cell isolation, and the cell analyzers FACSymphony A1 (BD), Canto II (BD) and Guave (Merck) were used for analysis. Data were depicted and analyzed using

(16) Hydrogel-Based Extracellular Vesicle Isolation Method from Various Biological SolutionsACS Applied Bio MaterialsJanuary 5, 2026Ryota Kajihara, Kyohei Tobinaga, Riho Tanigaki, Koichi Ute, Tatsuya Tominagafluorophore-conjugated antibodies targeting specific EV markers. Flow cytometry was conducted using a BD FACSymphony A1 Cell Analyzer with BD Small Particle Detector (Becton, Dickinson and Company), using side scatter signal detector

(17) Imaging CRISPR-edited CAR-T cell therapies with optical and positron emission tomography reportersTheranosticsJanuary 1, 2026Rafael Enrique Sanchez-Pupo, John Joseph Kelly, Nourhan Shalaby, Ying Xia, Francisco Manuel Martinez-Santiesteban, Jasmine Lau, Ivy Elizabeth Verriet, Matthew Stefan Fox, Justin Wesley Hicks, Jonathan Dale Thiessen, et al.after nucleofection and rAAV6 transduction. Immunophenotyping by flow cytometry was performed using a FACSymphony™ A1 cytometer and analyzed with FlowJo v10.10.0 for macOS. Where applicable, rhCD19-Fc-Chimera-Alexa Fluor®

(18) Open-source cell culture automation system with integrated cell counting for passaging microplate culturesPNAS NexusDecember 30, 2025Greg Courville, Shivanshi Vaid, Alexis Toruño, Paul Lebel, Joana P Cabrera, Preethi Raghavan, Axel Jacobsen, George R R Bell, Manuel D Leonetti, Rafael Gómez-Sjöbergcounting chamber slides (C10228). Flow cytometry measurements were performed using a Becton Dickinson FACSymphony A1 flow cytometer with High Throughput Sampler, using FACSDiva software for data acquisition. Analysis

(19) Neuropilin-1 expression modulates infection susceptibility to murine cytomegalovirus at the materno-fetal interfaceJournal of VirologyDecember 23, 2025Luís Fonseca Brito, Eléonore Ostermann, Julian Kottlau, Silvia Tödter, Renke Brixel, Roland Schüle, Maria Emilia Solano, Wolfram Brune, Felix Rolf Stahlplaque assay on M2-10B4 cells. Flow cytometry Cells were acquired on a LSRFortessa Cell Analyzer or FACSymphony A1 Cell Analyzer (BD Biosciences). The following antibodies were used for stainings: CD45-APC (30-F11),

(20) The mechanosensitive channel TRPV4 inhibits pulmonary inflammation by limiting NF-κB signaling in alveolar macrophagesScience SignalingDecember 23, 2025Adam M. Boulton, Megan E. Grund, Yuxin Wang, Erica M. Orsini, Yan Liu, Susamma Abraham, Lisa M. Grove, Ryan Musich, Caitlin M. Snyder, Haley Ricci, et al.was performed in cluster tubes of 250 μl volume on ice incubated for 30 min. Samples were run on a FACSymphony A1 (BD Biosciences) flow cytometer with standard configuration with 50,000 to 100,000 events acquired.

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Detection of extracellular vesicles (EVs) using the BD FACSymphony™ A1 Cell Analyzer
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This study offers a compelling look at how to overcome the limitations of conventional flow cytometry. Leveraging the BD FACSymphony™ A1 Cell Analyzer with its Small Particle Detector (SPD), researchers demonstrate powerful strategies to resolve tiny, low-refractive particles from background noise. Using the MIFlowCyt-EV framework and innovative techniques like “OR” thresholding, the study showcases how improved instrumentation and experimental design can dramatically enhance sensitivity, resolution, and dynamic range for small particle analysis. Robust performance is validated across multiple instruments and time points, providing confidence in reproducibility. Perfect for scientists seeking to refine EV detection, gain accurate size and fluorescence data, and publish high-quality, reproducible results. Read More
Flow cytometric analysis of extracellular vesicles
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Take your extracellular vesicle (EV) flow cytometry to the next level with this comprehensive guide from BD Biosciences. Whether you're setting up from scratch or optimizing existing workflows, this document walks you through a robust protocol using the BD FACSymphony™ A1 Cell Analyzer, BD FACSDiva™ Software, FlowJo™, and the Rosetta Calibration Plugin. Read More