BioProfile FLEX2 Automated Cell Culture Analyzer from Nova Biomedical

Product Specs
ItemBioProfile FLEX2 Automated Cell Culture Analyzer
CompanyNova Biomedical
Price Get Pricing Get Quote for this Product
Catalog NumberBioProfile FLEX2
QuantityEA
Analysis Time120 to 270 s
TypeChemical Analyzer
Sample Type24-sample tray, tube, 96-well plate, syringe, On-line Autosampler for up to 10 bioreactors
Dimensions(WxDxH) 17 x 23.5 x 25 in (43 x 60 x 64 cm)
ApplicationsMonitoring the progress of bioreactor runs, Determining consumption and production of key metabolites, Identification of growth-limiting nutrients, Design of feeding strategies, Calibration of bioreactor probes, Measurement of cellular respiration, Balancing media electrolytes, Control levels of waste product.
Time2.0 minutes (Gases only)
2.0 minutes (Chemistry only)
4 minutes (Osmolality only)
4 minutes (Cell Density/Viability)
4.5 minutes (All 16 tests run simultaneously)
Sample Volume135 to 275 µL

Specifications/Features

Glutamine, Glutamate, Glucose, Lactate, Ammonium, pH, PO2, PCO2, Sodium, Potassium, Ionized Calcium, Osmolality, Total Cell Density, Viable Cell Density, Cell Viability, Cell Diameter

Add to Compare List

Nova Biomedical

Nova Biomedical Corporation

200 Prospect Street

Waltham, Massachusetts

United States

Phone: 800-822-0911
800-545-NOVA (6682)
781-894-5915

Company Profile

Website: http://www.novabiomedical.com

(1) A novel hybrid bioprocess strategy addressing key challenges of advanced biomanufacturingFrontiers in Bioengineering and BiotechnologyJune 30, 2023Lucas Nik Reger, Martin Saballus, Annika Kappes, Markus Kampmann, Rene H. Wijffels, Dirk E. Martens, Julia NiemannCedex HiRes (Roche), metabolic parameters and pH by ABL800 basic (Radiometer) and ammonia analyzed via BioProfile® FLEX2™ (Nova Biomedical). Residual samples were centrifuged at 6600 × g for 5 min (Centrisart A-14, Sartorius)

(2) Process intensification for lentiviral vector manufacturing using tangential flow depth filtrationMolecular Therapy - Methods & Clinical DevelopmentJune 8, 2023Robert M. Tona, Reeti Shah, Kimberly Middaugh, Justin Steve, João Marques, Blair R. Roszell, Cindy JungCoulter, Brea, CA). Offline measurements of dissolved gases and key metabolites were performed using BioProfile FLEX2 (Nova Biomedical, Waltham, MA). Turbidity measurements were taken using a 2100Q portable turbidity meter

(3) Phenotypic, metabolic, and biogenesis properties of human stem cell-derived cerebellar spheroidsScientific ReportsDecember 1, 2022Timothy Hua, Chang Liu, Sonia Kiran, Kelly Gray, Sunghoon Jung, David G. Meckes, Yan Li, Qing-Xiang Amy SangRCP condition. For control and RCP groups, the spent media were collected and analyzed (n = 4) with a BioProfile Flex2 cell culture analyzer (Nova Biomedical) for metabolite analysis. Flow cytometry The spheroids were

(4) Development of a perfusion process for serum-free adenovirus vector herpes zoster vaccine productionAMB ExpressDecember 1, 2022Yang Sun, Lingling Huang, Jianqi Nie, Kai Feng, Yupeng Liu, Zhonghu Baistaining method. The concentrations of glucose, glutamine, ammonium, and lactate were measured using the BioProfile Flex2 Analyzer (Nova Biomedical, USA). The adenovirus titer was detected using the QuickTiter™ Adenovirus

(5) Onâ€line untargeted metabolomics monitoring of an Escherichia coli succinate fermentation processBiotechnology and BioengineeringOctober 1, 2022Joan Cortadaâ€Garcia, Jennifer Haggarty, Tessa Moses, RÃ³nÃ¡n Daly, Susan Alison Alison Arnold, Karl Burgessdecades. Some of these are based on enzymatic analysis—such as the Cedex Bio® Analyzer (Roche) and the BioProfile FLEX2 (Nova biomedical) (Morris et al., 2021; Obaidi et al., 2021)—while others use the so‐called “miniaturized”

(6) Lactate increases stemness of CD8 + T cells to augment anti-tumor immunityNature CommunicationsSeptember 6, 2022Qiang Feng et al.plasma by centrifugation for 10 mins at 2000 g at 4 oC. Lactate concentration in plasma was measured by BioProfile® FLEX2 (Nova Biomedical). Tumor free C57BL/6 J mice were used for this set of experiment. pH in blood Mouse

(7) Transcriptomic analysis reveals mode of action of butyric acid supplementation in an intensified CHO cell fedâ€batch processBiotechnology and BioengineeringSeptember 1, 2022Markus Schulze, Yadhu Kumar, Merle Rattay, Julia Niemann, Rene H. Wijffels, Dirk E. Martensmetabolite levels (glucose, lactate, ammonia, and l‐glutamine) and osmolality were measured using a BioProfile® FLEX2 (Nova Biomedical). Cell‐free supernatants (stored at −20°C) were used for product quantification via

(8) Automated Data Generation for Raman Spectroscopy Calibrations in Multi-Parallel Mini BioreactorsSensors (Basel)April 28, 2022Alexander Graf, Angus Woodhams, Michael Nelson, Douglas D. Richardson, Steven M. Short, Mark Brower, Marek Hoehsediverted to a waste bottle. Culture samples from the mini bioreactors were analyzed using an integrated Bioprofile® FLEX2 (Nova Biomedical Corporation, Waltham, MA, USA) automated cell culture analyzer with an External Sample

(9) N-1 Perfusion Platform Development Using a Capacitance Probe for BiomanufacturingBioengineering (Basel)March 22, 2022Emily S. C. Rittershaus, Matthew S. Rehmann, Jianlin Xu, Qin He, Charles Hill, Jeffrey Swanberg, Michael C. Borys, Zheng-Jian Li, Anurag Khetandissolved gases for the manufacturing-scale bioreactors (i.e., 500 L scale) were measured using the BioProfile FLEX2 (Nova Biomedical). 2.3. Laboratory Scale N-1 Perfusion Bioreactor with Online Capacitance Control Laboratory

(10) The efficient development of a novel recombinant adenovirus zoster vaccine perfusion production processVaccineMarch 18, 2022Jianqi Nie, Yang Sun, Kai Feng, Lingling Huang, Ye Li, Zhonghu Baianalyzer (Roche Diagnostics, Switzerland). The pH of the shake flasks were measured by the gas sensors of BioProfile Flex2 Analyzer (Nova Biomedical, USA). The titer (infectious units, IFUs) of adenovirus was quantified by

(11) Exploring the limits of conventional small-scale CHO fed-batch for accelerated on demand monoclonal antibody productionBioprocess and Biosystems EngineeringFebruary 1, 2022Amélie Mahé, Alexandra Martiné, Séverine Fagète, Pierre-Alain Girodviability, glucose, lactate, glutamine, glutamate, ammonia, pH, pO2, and pCO2 were measured using a Bioprofile FLEX2 (Nova Biomedical, USA). Cells were grown for 14 days. Off-process product characterization assays Microbioreactors

(12) Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch cultureScientific ReportsDecember 1, 2021Menglin Shang, Taehong Kwon, Jean-Francois P. Hamel, Chwee Teck Lim, Bee Luan Khoo, Jongyoon Handaily to measure viable cell concentration and cell diameter via an automated cell culture analyser (BioProfile FLEX2, Nova Biomedical, USA). Four different pressure conditions, 0, 30, 60 and 90 mmHg, were applied to the

(13) Novel Platform for Regulation of Extracellular Vesicles and Metabolites Secretion from Cells Using a Multi-Linkable Horizontal Co-Culture PlateMicromachines (Basel)November 21, 2021Takeo Shimasaki et al.supernatant was filtered at each growth phase through a 0.22 μm membrane and directly analyzed with a BioProfile FLEX2 analyzer following the manufacturer’s instructions (Nova Biomedical, Inc., Waltham, MA, USA). The measurements

(14) A 3D Bioprinted In Vitro Model of Pulmonary Artery Atresia to Evaluate Endothelial Cell Response to MicroenvironmentAdvanced Healthcare MaterialsOctober 1, 2021Martin L. Tomov et al.and perfusion PAA constructs (n = 3) throughout the culture period and analyzed (400 μL) using the BioProfile FLEX2 Automated Cell Culture Analyzer (Nova Biomedical, USA). This technique measured pH levels, metabolite concentrations (glucose, glutamine,

(15) Compartmentalized Proteomic Profiling Outlines the Crucial Role of the Classical Secretory Pathway during Recombinant Protein Production in Chinese Hamster Ovary CellsACS OmegaMay 18, 2021Saumel Pérez-Rodriguez, Tune Wulff, Bjørn G. Voldborg, Claudia Altamirano, Mauricio A. Trujillo-Roldán, Norma A. Valdez-Cruzammonium, sodium, potassium, and calcium, and pH were measured in supernatants every 24 h by using BioProfile FLEX2 Automated Cell Culture Analyzer (Nova Biomedical, Waltham, MA, USA). Specific consumption or production rates were calculated from the

(16) 4-(2,5-Dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culturePLOS ONEApril 22, 2021Yuichi Aki, Yuta Katsumata, Hirofumi Kakihara, Koichi Nonaka, Kenshu Fujiwaracell culture Metabolite analysis Glucose and lactate concentrations in the cultures were analyzed by BioProfile FLEX2 (Nova Biomedical, Waltham, MA, USA) according to the manufacturer’s protocol. Concentration of mAb Cell culture

(17) Streptococcus pyogenes upregulates arginine catabolism to exert its pathogenesis on the skin surfaceCell ReportsMarch 30, 2021Yujiro Hirose et al.ΔarcA cultured in THY broth was filtered through a 0.22 μm membrane, then and directly analyzed with BioProfile® FLEX2 analyzer following manufacturer’s instruction (Nova Biomedical, Inc., Waltham, MA, USA). Measurement

(18) Bioreactor Parameters for Microcarrier-Based Human MSC Expansion under Xeno-Free Conditions in a Vertical-Wheel SystemBioengineering (Basel)July 8, 2020Josephine Lembong, Robert Kirian, Joseph D. Takacs, Timothy R. Olsen, Lye Theng Lock, Jon A. Rowley, Tabassum AhsanFollowing dissociation, the number of cells was counted. The medium supernatant was analyzed using the BioProfile FLEX2 Analyzer (Nova Biomedical, Waltham, MA, USA) to monitor the metabolite and waste concentration. Visualization

(19) Nutrient supplementation strategy improves cell concentration and longevity, monoclonal antibody production and lactate metabolism of Chinese hamster ovary cellsBioengineeredApril 1, 2020Saumel Pérez-Rodriguez, María de Jesús Ramírez-Lira, Mauricio A. Trujillo-Roldán, Norma A. Valdez-Cruzpotassium and calcium concentrations in culture supernatants were measured at the end of cultures by using BioProfile® FLEX2™ Automated Cell Culture Analyzer (Nova Biomedical, Waltham, MA, the USA). Quantification of overall specific productivity For calculation

(20) Computational fluid dynamics modeling, a novel, and effective approach for developing scalable cell therapy manufacturing processesBiotechnology and BioengineeringDecember 1, 2019Mehdi Shafa, Krishna M Panchalingam, Tylor Walsh, Thomas Richardson, Behnam Ahmadian Baghbaderania lower volume of 3.0 ml was withdrawn. Metabolites, nutrients and pH data were acquired using the BioProfile® FLEX2™ (Nova Biomedical). Sampled aggregates were placed in a six‐well culture plate (ThermoFisherScientific)

Be the First to Write a Review!

Sensors (Basel)

BioProfile FLEX2 Automated Cell Culture Analyzer from Nova Biomedical

Description

Specifications/Features

Nova Biomedical

Citations (21)

Reviews

BioProfile FLEX2 Automated Cell Culture Analyzer from Nova Biomedical

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map