Ac-DEVD-AFC from Enzo Life Sciences, Inc.

Description

Fluorogenic substrate for caspase-3 (CPP32), with a Km=9.7µM) and related cysteine proteases. Sequence is based on PARP cleavage at Asp216 for caspase-3. Similar to Ac-DEVD-AMC (Prod. No. ALX-260-031) but the AFC fluorophore has a greater Stokes’ shift upon cleavage than AMC. Reaction can be monitored quantitatively or visually using a hand-held long-UV lamp and visualizing a blue to green shift in fluorescence upon cleavage. Ex.: 400nm, Em.: 505nm

Product Specs
ItemAc-DEVD-AFC
CompanyEnzo Life Sciences, Inc.
Price $196.00Get Pricing Get Quote for this Product
Catalog NumberALX-260-032-M005
FormulaC₃₀H₃₄F₃N₅O₁₃
Sample TypeWhite to off-white powder
Purity≥96% (HPLC)
Molecular Weight729.6
Quantity5 mg
Molecule NameAc-DEVD-AFC
TypePeptide

Sequence

Ac-Asp-Glu-Val-Asp-AFC (AFC = 7-Amino-4-trifluoromethylcoumarin)

Specifications/Features

Caspase-3 substrate

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Enzo Life Sciences, Inc.

10 Executive Blvd.

Farmingdale, NY 11735

United States

Phone: 800-942-0430
Fax: 610-941-9252

Company Profile

Website: www.enzo.com

(1) Sublethal cytochrome c release generates drug-tolerant persister cellsCellSeptember 1, 2022Kalkavan H, Chen MJ, Crawford JC, Quarato G, Fitzgerald P, Tait SWG, Goding CR, Green DR.,Thermo Scientific,Cat# PV5856 3X FLAG® Peptide,Sigma-Aldrich,Cat# F4799 AC DEVD AFC,Enzo Life Sciences,Cat# ALX260032M005 Cytochrome c from Saccharomyces cerevisiae,Sigma-Aldrich,Cat# C2436 "Cytochrome C, equine",Sigma-Aldrich,Cat#

(2) Characterization of Anti-Cancer Activities of Violacein: Actions on Tumor Cells and the Tumor MicroenvironmentFrontiers in OncologyMay 11, 2022Charlotte Dahlem, Shilpee Chanda, Jan Hemmer, Hanna S. Schymik, Michael Kohlstedt, Christoph Wittmann, Alexandra K. Kiemer96 well plates. 90 µl substrate solution were added (55 µM of fluorogenic substrate Ac-DEVD-AFC (Enzo, #ALX-260-032-M005), 50 mM HEPES, 0.1% [w/v] CHAPS, 1% [w/v] sucrose, 10 mM DTT, pH 7.5). Free AFC (7-amino-4-trifluoro-methyl

(3) Delineation of cell death mechanisms induced by synergistic effects of statins and erlotinib in non-small cell lung cancer cell (NSCLC) linesScientific ReportsDecember 1, 2020Alexander Otahal, Duygu Aydemir, Erwin Tomasich, Christoph MinichsdorferDTT) containing 50 µM of the 7-amino-4-trifluoro-methylcoumarin (AFC)-conjugated substrate (ENZO, #ALX-260–032-M005). Samples were incubated for 90 minutes at 37 °C in the dark, then fluorescence was measured at 535

(4) Toll-Like Receptor 2 Release by Macrophages: An Anti-inflammatory Program Induced by Glucocorticoids and LipopolysaccharideFrontiers in immunologyJanuary 1, 2019Hoppstädter J, Dembek A, Linnenberger R, Dahlem C, Barghash A, Fecher-Trost C, Fuhrmann G, Koch M, Kraegeloh A, Huwer H, et al.black 96 well plate (TPP). 90 μL substrate solution (55 μM of fluorogenic substrate Ac-DEVD-AFC (Enzo, #ALX-260-032-M005) 50 mM HEPES, 0.1% [w/v] CHAPS, 1% [w/v] sucrose, 10 mM DTT, pH 7.5) were added, and the production

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Ac-DEVD-AFC from Enzo Life Sciences, Inc.

Description

Sequence

Specifications/Features

Enzo Life Sciences, Inc.

Citations (4)

Reviews

Ac-DEVD-AFC from Enzo Life Sciences, Inc.

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