GloSensor cAMP Reagent from Promega

Product Specs
ItemGloSensor cAMP Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1291
Quantity250mg
Methodautomated
TypeEnzyme

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammationCell ReportsJanuary 27, 2026Jun Nagai, Madeline M. Hastings, Airi Nishida, Sreyashi Majumdar, Kendall Zaleski, Sofia A. Marshall, Junrui Lin, Mira A.S. Saliman, Yumi Tani, Yamato Murakami, et al.SDS-Sample Buffer (4X, Reducing)",Boston Bioproduct,BP-110R GloSensor™ Technology Assay Reagent,Promega,E1291 Nano-Glo® Live Cell Substrate,Promega,N205 Sodium chloride solution,Sigma-Aldrich,S8776 Patterson

(2) Interplay between T3SS effectors, ExoY activation, and cGMP signaling in Pseudomonas aeruginosa infectionNat CommunDecember 2, 2025Vincent Deruelle, Gabrielle Dupuis, Dorothée Raoux-Barbot, Elysa Lim, Roberto Ponce-López, Magda Teixeira Nunes, Daniel Ladant, Louis Renault, Undine Mecholdreplaced with 100 µL / well of phenol red-free RPMI containing 5% GloSensor cAMP reagent (Promega, #E1291). Luminescence was monitored every 5 min for 4 h at 24 °C with an automated plate reader (Spark 10 M

(3) A Chemically Stable Photocaged NoradrenalineACS Chem NeurosciAugust 6, 2025Stanley A. Buczynski, Amy Li, Janie Chang-Weinberg, Shayra S. Nawsheen, Matthew R. BanghartpH 7.2, 2 g/L d-glucose), followed by addition of 25 μL of 3 mM luciferin (Assay Reagent, Promega, E1291) and incubated for 2 h at 37 °C. 25 μL of 4× drug agonist solutions was added, and immediately, luminescence

(4) Molecular mechanism of human α1A-adrenoceptor inhibition by Mamba snake toxin AdTx1Commun BiolJuly 16, 2025Mingyu Shi, Shuhao Zhang, Angqi Zhu, Yosuke Toyoda, Fang Kong, Xiaoou Sun, Xinyu Xu, Chuangye Yan, Xiangyu LiuMicrobeta2 scintillation counter. GloSensor cAMP assay A GloSensor cAMP kit from Promega (Cat. No. E1291) was used in this study. In brief, the pGloSensor™-22F plasmid, receptor plasmid, and Gsq plasmid were

(5) Regulation of CXCR4 function by S1P1 through heteromerizationCell Commun SignalFebruary 26, 2025Hyun-Tae Kim, Jae-Yeon Jeong, Won-Ki HuhcAMP assay Quantification of cAMP production was conducted using the GloSensor cAMP assay (Promega, #E1291). HEK293A cells were seeded in 6-well plates and transfected with plasmids encoding GloSensor-22 F,

(6) Downregulation of miR-214 promotes dilated Cardiomyopathy Progression through PDE5A-Mediated cGMP regulationScientific ReportsNovember 14, 2024Jingjing Yan, Xinzhou Wang, Panxia Cao, Qiaozhi Li, Hong Wugene. Transfected cells were harvested and seeded in a 96-well plate. The GloSensor reagent (Cat. # E1291, Promega) was added to the culture medium 30 min prior to measurement. Test samples or treatments were

(7) Phase 1, first-in-human study of TYRP1-TCB (RO7293583), a novel TYRP1-targeting CD3 T-cell engager, in metastatic melanoma: active drug monitoring to assess the impact of immune response on drug exposureFrontiers in OncologyMarch 21, 2024Anna Spreafico, Eva Muñoz Couselo, Anja Irmisch, Juliana Bessa, George Au-Yeung, Oliver Bechter, Inge Marie Svane, Miguel F. Sanmamed, Valentina Gambardella, Meredith McKean, et al.well, corresponding to 70% of the total volume. Additionally, 2% end-volume of GloSensor cAMP Reagent (E1291, Promega) was added to each well. After 4 h incubation time, luminescence was measured using a Tecan

(8) REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein BiosensorsBiosensors (Basel)July 28, 2023Katrijn Boon, Nathan Vanalken, Eef Meyen, Dominique Schols, Tom Van Loysupplemented with 300 µM 3-isobutyl-1-methylxanthine (#I7018, MERCK) and 2% GloSensor cAMP reagent (#E1291, Promega) was then added to the cells (100 µL/well). After 2 h of incubation at 37 °C, baseline luminescence

(9) Antagonistic interactions between odorants alter human odor perceptionCurrent BiologyJune 1, 2023Yosuke Fukutani, Masashi Abe, Haruka Saito, Ryo Eguchi, Toshiaki Tazawa, Claire A. de March, Masafumi Yohda, Hiroaki Matsunamiassays,Critical commercial assays,Critical commercial assays Glosensor cAMP assay,Promega Corporation,Cat# E1291 CellTiter-Glo 2.0 Cell Viability Assay,Promega Corporation,Cat# G9241 Experimental models: Cell lines,Experimental models

(10) In vivo photopharmacology with a caged mu opioid receptor agonist drives rapid changes in behaviorNature MethodsMay 1, 2023Xiang Ma, Desiree A. Johnson, Xinyi Jenny He, Aryanna E. Layden, Shannan P. McClain, Jean C. Yung, Arianna Rizzo, Jordi Bonaventura, Matthew R. BanghartCNV-Y-DAMGO) solutions for 15 min at 37 °C. Finally, 25 μL of 4 mM luciferin (Assay Reagent, Promega, E1291) supplemented with isoproterenol at a final concentration of 200 nM was added, and luminescence counting

(11) The Third Extracellular Loop of Mammalian Odorant Receptors Is Involved in Ligand BindingInternational Journal of Molecular SciencesOctober 18, 2022Tammy Shim, Jody Pacalon, Won-Cheol Kim, Xiaojing Cong, JÃ©rÃ©mie Topin, JÃ©rÃ´me Golebiowski, Cheil Moonindependent media (#18045088, Thermo Fisher Scientific, Waltham, MA, USA) with GloSensorTM cAMP Reagent (#E1291, Promega, Madison, WI, USA) before exposure to solvent or odorants. Each odorant was diluted into CO2

(12) Cellular Electrical Impedance as a Method to Decipher CCR7 Signalling and Biased AgonismInternational Journal of Molecular SciencesAugust 10, 2022Nathan Vanalken, Katrijn Boon, Jordi Doijen, Dominique Schols, Tom Van LoyµM 3-isobutyl-1-methylxanthine (#I7018, Merck, Darmstadt, Germany) and 2% GloSensor cAMP reagent (#E1291, Promega, Madison, MD, USA) for 2 h at 37 °C. The plate was transferred to the FLIPR Tetra (Molecular Devices

(13) The CNS-penetrant soluble guanylate cyclase stimulator CYR119 attenuates markers of inflammation in the central nervous systemJournal of neuroinflammationSeptember 18, 2021Correia SS, Liu G, Jacobson S, Bernier SG, Tobin JV, Schwartzkopf CD, Atwater E, Lonie E, Rivers S, Carvalho A, et al.chamber with 5% CO2. The next day, medium was replaced with 2 mM GloSensor reagent (Promega, Cat. #E1291) at 40 µL/well in CO2-independent medium (Gibco Cat. #18045-088). Cells were treated for 90 min (min)

(14) Human GPR17 missense variants identified in metabolic disease patients have distinct downstream signaling profilesJournal of Biological ChemistryJuly 1, 2021Jason M. Conley, Hongmao Sun, Kristin L. Ayers, Hu Zhu, Rong Chen, Min Shen, Matthew D. Hall, Hongxia Renwere aspirated and replaced with 100 μl/well equilibration medium (2% Promega GloSensor cAMP reagent [E1291; Promega Corporation] in CO2-independent medium) and incubated at room temperature for 2 h. Baseline

(15) Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammationSignal transduction and targeted therapyFebruary 28, 2021Du X, Li F, Zhang C, Li N, Huang H, Shao Z, Zhang M, Zhan X, He Y, Ju Z, et al.later, cell medium were replaced by 50 μL of CO2-independent medium with 2% GloSensor cAMP Reagent (E1291, Promega) and incubated for 2 h at 37 °C. Then cells were stimulated with mCCL6 at a concentration ranging

(16) Ligand recognition and allosteric regulation of DRD1-Gs signaling complexesCellFebruary 10, 2021Peng Xiao, Wei Yan, Lu Gou, Ya-Ni Zhong, Liangliang Kong, Chao Wu, Xin Wen, Yuan Yuan, Sheng Cao, Changxiu Qu, et al.kit,Cisbio,Cat# 62AM4PEB Q5 site directed mutagenesis kit,NEB,Cat# E0554S cAMP Glosensor Assay,Promega,Cat# E1291 Deposited data,Deposited data,Deposited data Fenoldopam-DRD1-Gs EM map and coordinate,This paper,EMDB:

(17) The CNS-Penetrant Soluble Guanylate Cyclase Stimulator CY6463 Reveals its Therapeutic Potential in Neurodegenerative DiseasesFrontiers in pharmacologyJanuary 1, 2021Correia SS, Iyengar RR, Germano P, Tang K, Bernier SG, Schwartzkopf CD, Tobin J, Lee TW, Liu G, Jacobson S, et al.chamber with 5% CO2. The next day, medium was replaced with 2 mM GloSensor reagent (Promega, Cat. #E1291) at 40 µl/well in CO2-independent medium (Gibco Cat. #18045-088). Cells were treated for 90 (min) at

(18) Orally active adenosine A(1) receptor agonists with antinociceptive effects in miceJournal of medicinal chemistryJuly 26, 2012Korboukh I, Hull-Ryde EA, Rittiner JE, Randhawa AS, Coleman J, Fitzpatrick BJ, Setola V, Janzen WP, Frye SV, Zylka MJ, et al.μM forskolin (Tocris) were added by Multimek. Seven minutes later, GloSensor cAMP reagent (Promega #E1291) containing 0.1% final luciferin and supplemented with 0.3% final NP40 (Tergitol, Sigma #NP40S) to permeabilize

Be the First to Write a Review!