GloSensor cAMP Reagent from Promega

Product Specs
ItemGloSensor cAMP Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1290
Methodautomated
Quantity25mg
Molecular Weight~68kDa (CDK9); ~67kDa (CyclinK)
TypeEnzyme

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) The Muscarinic Acetylcholine Receptor in Dermal Papilla Cells Regulates Hair GrowthInternational Journal of Molecular SciencesFebruary 9, 2026Gary K. W. Yuen, K. W. Leung, Queenie W. S. Lai, Maggie S. S. Guo, Alex X. Gao, Janet Y. M. Ho, Harry C. T. Chu, Karl W. K. Tsimpromoter, was purchased from Upstate Biotechnology (Erie, PA, USA). The pGloSensor-22F cAMP plasmid (Cat: E1290) was purchased from Promega (Madison, WI, USA). GloSensor™ luciferase contains cAMP binding domain fused

(2) Structural insights into the mechanism of activation and inhibition of the prostaglandin D2 receptor 1Nat CommunOctober 8, 2025Behnaz Davoudinasab, Aleksey Raskovalov, Woojin Lee, Donggyun Kim, Heesoo Kim, Jordy Homing Lam, Gye Won Han, Vsevolod Katritch, Vadim CherezovDNA(µg):TransIT(µL)). The following day, 200 µL of 30 mg/mL GloSensor™ Assay Reagent (Promega, Cat# E1290) dissolved in 10 mM HEPES pH 7.5 was diluted in 10 mL of Assay Media, resulting in a final GloSensor

(3) Glucagon Receptor Deficiency Causes Early-Onset Hepatic SteatosisDiabetesOctober 1, 2025Tessa M. Cacciottolo, Katherine Lawler, Kevin M. Méndez-Acevedo, Edson Mendes de Oliveira, Adam Syanda, Elana Henning, Julia M. Keogh, Rebecca Bounds, Miriam Smith, Daniyal Ashraf, et al.Lipofectamine 2000. After 24 h, medium was replaced with 90 µL of 2% v/v GloSensor cAMP reagent (Promega, E1290), and cells were incubated for 2 h at 37°C. Basal luminescence was measured using a Spark M1000 plate

(4) Intracrine FFA4 signaling controls lipolysis at lipid dropletsNature Chemical BiologyAugust 5, 2025Shannon L. O’Brien, Emma Tripp, Natasja Barki, Elodie Blondel-Tepaz, Gabrielle Smith, Adam Boufersaoui, Jennie Roberts, Jeremy A. Pike, Joao Correia, Tamara Miljus, et al.Nano-Glo luciferase assay substrate (N1120), HaloTag Janelia Fluor 646 (GA1120) and GloSensor cAMP assay (E1290) were from Promega. SNAP-Cell 647 (S9102S), BamHI (R0136), EcoRI (R3101), AsiSI (R0630) and XhoI (R0146)

(5) Intrinsic Molecular Proton Sensitivity Underlies GPR4 Effects on Retrotrapezoid Nucleus Neuronal Activation and CO2-Stimulated BreathingJ NeurosciSeptember 4, 2024Elizabeth C. Gonye, Yingtang Shi, Keyong Li, Rachel T. Clements, Wenhao Xu, Douglas A. BaylissHank's balanced salt solution (HBSS; Invitrogen 14175-095) containing 2% v/v GloSensor Reagent (Promega E1290). Cells were equilibrated for 2 h at 37°C/5% CO2. After equilibration, the solution was replaced with

(6) Diversely evolved xibalbin variants from remipede venom inhibit potassium channels and activate PKA-II and Erk1/2 signalingBMC BiologyJuly 29, 2024Ernesto Lopes Pinheiro-Junior, Ehsan Alirahimi, Steve Peigneur, Jörg Isensee, Susanne Schiffmann, Pelin Erkoc, Robert Fürst, Andreas Vilcinskas, Tobias Sennoner, Ivan Koludarov, et al.HEK293T cells were incubated in DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany). Induction and inhibition assay were performed in two steps with the same

(7) Understanding the genetic complexity of puberty timing across the allele frequency spectrumNature GeneticsJuly 1, 2024Katherine A. Kentistou, Lena R. Kaisinger, Stasa Stankovic, Marc Vaudel, Edson Mendes de Oliveira, Andrea Messina, Robin G. Walters, Xiaoxi Liu, Alexander S. Busch, Hannes Helgason, et al.cell media were replaced by 90 ml of fresh DMEM with 2% vol/vol GloSensorTM cAMP reagent (Promega, E1290) and incubated for 120 min at 37 °C. Firefly luciferase activity was measured at 37 °C and 5% CO2 using

(8) The Discovery of Novel α2a Adrenergic Receptor Agonists Only Coupling to Gαi/O Proteins by Virtual ScreeningInternational Journal of Molecular SciencesJune 30, 2024Peilan Zhou, Fengfeng Lu, Huili Zhu, Beibei Shi, Xiaoxuan Wang, Shiyang Sun, Yulei Li, Ruibin Suday, the cell media were replaced by 90 μL of fresh DMEM with 2% v/v GloSensor cAMP reagent (Promega, E1290) and incubated for 60 min at 37 °C. Before drug stimulation, an initial measurement of the baseline

(9) Systematic characterization of multi-omics landscape between gut microbial metabolites and GPCRome in Alzheimer’s diseaseCell ReportsMay 28, 2024Yunguang Qiu, Yuan Hou, Dhruv Gohel, Yadi Zhou, Jielin Xu, Marina Bykova, Yuxin Yang, James B. Leverenz, Andrew A. Pieper, Ruth Nussinov, et al.pGloSensor™-22F cAMP plasmid,Promega,E1171 GPR84 plasmid,Addgene,RRID: Addgene_66377 GloSensor™ cAMP Reagent,Promega,E1290 DMEM,Gibco,10313021 Fetal Bovine Serum,Gibco,10270106 ,, "Chemicals, peptides, and recombinant proteins",, ,, Phenethylamine,Sigma,#128945 Agmatine

(10) Low resting metabolic rate and increased hunger due to β-MSH and β-endorphin deletion in a canine modelSci AdvMarch 8, 2024Marie T. Dittmann, Gabriella Lakatos, Jodie F. Wainwright, Jacek Mokrosinski, Eloise Cross, I. Sadaf Farooqi, Natalie J. Wallis, Lewis G. Halsey, Rory Wilson, Stephen O'Rahilly, et al.medium was replaced with 90 μl of complete media with 2% GloSensor substrate (Promega, catalog no. E1290). This was left to incubate for 2 hours at 37°C, and 5% CO2 before measurements were made using a Tecan

(11) Novel Scaffold Agonists of the α2A Adrenergic Receptor Identified via Ensemble-Based StrategyMoleculesFebruary 29, 2024Shiyang Sun, Pengyun Li, Jiaqi Wang, Dongsheng Zhao, Tingting Yang, Peilan Zhou, Ruibin Su, Zhibing Zheng, Song LiThe medium was replaced with 90 μL of fresh DMEM containing 2% v/v GloSensor cAMP reagent (Promega, E1290), then incubated for 60 min at 37 °C. The baseline signal was initially recorded before the cells were

(12) Expression of endogenous epitope-tagged GPR4 in the mouse braineNeuroFebruary 26, 2024Elizabeth C. Gonye, Alexandra V. Dagli, Natasha N. Kumar, Rachel T. Clements, Wenhao Xu, Douglas A. Baylissremoved and replaced with HBSS (Invitrogen 14175-095) containing 2% v/v GloSensor Reagent (Promega E1290). Cells were equilibrated for 2 h at 37°C/5% CO2. After equilibration, GloSensor reagent solution was

(13) Eyeless razor clam Sinonovacula constricta discriminates light spectra through opsins to guide Ca(2+) and cAMP signaling pathwaysThe Journal of biological chemistryJanuary 1, 2024Kong F, Ran Z, Zhang M, Liao K, Chen D, Yan X, Xu J.was replaced with the CO2-independent DMEM containing 10% FBS and 2% GloSensor cAMP reagent (Promega, E1290). Subsequently, the cells were incubated for an additional 2 h before luminescence measurement commenced.

(14) In vivo photopharmacology with light-activated opioid drugsNeuronDecember 20, 2023Shannan P. McClain, Xiang Ma, Desiree A. Johnson, Caroline A. Johnson, Aryanna E. Layden, Jean C. Yung, Susan T. Lubejko, Giulia Livrizzi, X. Jenny He, Jingjing Zhou, et al.,Hello Bio,Cat#HB2409 CTAP,Tocris,Cat#1560 Critical commercial assays,, GloSensor™ cAMP Assay,Promega,Cat#E1290 NanoBiT® barrestin Recruitment Assays,Promega,Cat#N2014 Deposited data,, Source data,This paper,N/A Experimental models

(15) Understanding the genetic complexity of puberty timing across the allele frequency spectrummedRxivJuly 21, 2023Katherine A Kentistou, Lena R Kaisinger, Stasa Stankovic, Marc Vaudel, Edson M de Oliveira, Andrea Messina, Robin G Walters, Xiaoxi Liu, Alexander S Busch, Hannes Helgason, et al.transfection, cell media were replaced by 90 mL of fresh DMEM with 2% v/v GloSensorTM cAMP Reagent (Promega, E1290) and incubated for 120 min at 37°C. Firefly luciferase activity was measured at 37°C and 5% CO2 using

(16) Investigation of adenosine A1 receptor-mediated β-arrestin 2 recruitment using a split-luciferase assayFrontiers in PharmacologyMay 30, 2023Luisa Saecker, Hanns Häberlein, Sebastian Frankencoelenterazine h (Prolume Ltd., Pinetop, AZ, United States; 50909-86-9), GloSensor™ cAMP Assay Reagent (Promega, E1290), Zeocin™ Selection Reagent (Thermo Fisher Scientific, R25001), Hygromycin B Gold (InvivoGen, San Diego,

(17) The Pharmacological Potential of Novel Melittin Variants from the Honeybee and Solitary Bees against Inflammation and CancerToxins (Basel)November 22, 2022Pelin Erkoc, Björn Marcus von Reumont, Tim Lüddecke, Marina Henke, Thomas Ulshöfer, Andreas Vilcinskas, Robert Fürst, Susanne SchiffmannThe supernatant was replaced by DMEM without phenol red supplemented with pGlo sensor cAMP reagent (E1290, Promega, Walldorf, Germany). Induction and inhibition assays were performed in two steps with the same

(18) Tyrosine 7.43 is important for mu-opioid receptor downstream signaling pathways activated by fentanylFrontiers in PharmacologySeptember 2, 2022Xiangyun Tian, Junjie Zhang, Shaowen Wang, Huan Gao, Yi Sun, Xiaoqian Liu, Wei Fu, Bo Tan, Ruibin Suh, cell media were replaced by 90 ml of fresh full DMEM with 2% v/v GloSensor cAMP Reagent (Promega, E1290) and incubated for 120 min at 37°C. Firefly luciferase activity was measured using Envision Multilabel

(19) S-nitrosylation is required for β(2)AR desensitization and experimental asthmaMolecular cellAugust 18, 2022Fonseca FV, Raffay TM, Xiao K, McLaughlin PJ, Qian Z, Grimmett ZW, Adachi N, Wang B, Hausladen A, Cobb BA, et al.Commercial Assays PARAMETER cAMP ELISA kit,R&D Systems,KGE012 GloSensor cAMP biosensor reagent,Promega,E1290 Aurum Total RNA Fatty and Fibrous Tissue Kit,Bio-Rad,7326830 iScript cDNA Synthesis Kit,Bio-Rad,1708890 Fast

(20) Chimeric GPCRs mimic distinct signaling pathways and modulate microglia responsesNature communicationsAugust 15, 2022Schulz R, Korkut-Demirbaş M, Venturino A, Colombo G, Siegert S.(GloSensor-22F; Promega; E2301)62. As luciferase substrate, a 100X stock solution of cAMP reagent (Promega; E1290) was prepared in 10 mM HEPES and stored at −80 °C. HEK cells were transfected with GPCR or empty vector

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