WesternBright Quantum HRP Substrate from Advansta

Product Specs
ItemWesternBright Quantum HRP Substrate
CompanyAdvansta
Price $420.00Get Pricing
Catalog NumberK-12042-D20
Quantity200ml
ApplicationsWestern blotting

Specifications/Features

WesternBright® Quantum® is formulated to take full advantage of the linear dynamic range of CCD imagers. It provides high sensitivity to detect low abundance proteins, and does not exhibit substrate depletion with high abundance proteins, so has a larger linear dynamic range than other commercially available substrates. A signal linear with respect to protein concentration over 3 orders of magnitude provides the ability to accurately quantify and compare protein amounts in your samples. Features- Sensitive - detect attomoles of protein per band. Quantitative - linear range of signal with respect to protein amount exceeds 3 orders of magnitude. Low background - for high signal to noise. Long lasting signal - image blots hours after substrate incubation. Versatile - Optimized for CCD imaging, and compatible with film detection

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) An estrogen receptor/E2F1/CDKN3 axis protects from UV-induced skin cancers in femalesEMBO ReportsMarch 24, 2026Céline Lukowicz, Carine Winkler, Catherine Roger, Joanna C Fowler, Yi-Chien Tsai, Joachim Meuli, Stéphanie Claudinot, Yun-Tsan Chang, Christoph Iselin, Philip H Jones, et al.Pierce™ BCA Protein Assay Kit,Life Technologies,Cat#23225 Advansta ECL WesternBright Quantum,Advansta,Cat#K-12042-D20 Amersham™ ECL Select™ Western Blotting Detection Reagent,Amersham,Cat#RPN2235 QIAGEN DNA micro kit,QIAGEN,Cat#56204 RNeasy

(2) Biostimulants in plant brassinosteroid hormone receptor BRI1 activation—a new system to evaluate activation capacityThe FEBS JournalAugust 28, 2025Maribel Marquina, Montserrat Ricart‐Fort, Rocío Díaz‐Parra, Sandra López‐Avilés, Tula Yance, Pablo Quirós, Elena Contreras, Ignasi Salaet, Sergio Atares, Rosa Aliguésecondary antibodies at room temperature for 1 h. A WesternBright™ Quantum detection kit (Cat. no.: K‐12042‐D20; Advansta) was used to detect bands. Quantification of western blot experiments and reproducibility of results

(3) Modeling oxaliplatin resistance in colorectal cancer reveals a SERPINE1-based gene signature (RESIST-M) and therapeutic strategies for pro-metastatic CMS4 subtypeCell Death DisJuly 16, 2025Stephen Qi Rong Wong, Mohua Das, Kenzom Tenzin, Niranjan Shirgaonkar, Huiwen Chua, Lin Xuan Chee, Ahpa Sae Yeoh, Astley Aruna Murugiah, Wei Yong Chua, Madelaine Skolastika Theardy, et al.Chemiluminescence imaging was done using the Azure 600 (Azure Biosystems) after incubation with HRP substrate (K-12042-D20, Advansta). List of antibodies and dilutions used is found in Table 2. ATAC-seq sample preparation

(4) Combined CSF1R Inhibition and Sensory Gamma Stimulation Provide Protective Effects in a Mouse Model of Alzheimer’s DiseasebioRxivJuly 16, 2025Chinnakkaruppan Adaikkan, Md Rezaul Islam, P. Lorenzo Bozzelli, Matt Sears, Cameron Parro, Ping-Chieh Pao, Na Sun, TaeHyun Kim, Karim Abdelaal, Mia Sedgwick, et al.membranes were treated with chemiluminescence substrates Western-Bright Quantum kit (Advansta, Cat # K-12042-D20) and the blots were visualized (Chem doc, Bio-Rad). Signal intensities were quantified using ImageJ

(5) Case Report: Functional investigation of the γENaC G532S mutation presenting as mild PHA-1B3Frontiers in medicineJanuary 1, 2025Centonze E, Girish M, van Bemmelen MX, Staub O, Subramaniam G, Kellenberger S.HRP signal was revealed using Western Bright Quantum detection reagent (Advansta, Menlo Park, CA, #K12042-D20) and detected using a Fusion Solo imaging system (Vilber Lourmat, Marne-la-Vallée, France). The exposure

(6) Association of GLOD4 with Alzheimer’s Disease in Humans and MiceJournal of Alzheimer's DiseaseSeptember 24, 2024Olga Utyro, Olga Włoczkowska-Łapińska, Hieronim Jakubowskiperoxidase (CST, #7074 S, 1:3,000). Positive signals were detected using Western Bright Quantum-Advansta K12042-D20 and GeneGnome XRQ NPC chemiluminescence detection system. Bands intensity was calculated using Gene

(7) Association of GLOD4 with Alzheimer’s Disease in Humans and MiceJ Alzheimers DisSeptember 16, 2024Olga Utyro, Olga Włoczkowska-Łapińska, Hieronim Jakubowskiperoxidase (CST, #7074 S, 1:3,000). Positive signals were detected using Western Bright Quantum-Advansta K12042-D20 and GeneGnome XRQ NPC chemiluminescence detection system. Bands intensity was calculated using Gene

(8) Homocysteine metabolites inhibit autophagy by upregulating miR-21-5p, miR-155-5p, miR-216-5p, and miR-320c-3p in human vascular endothelial cellsSci RepMarch 26, 2024Łukasz Witucki, Hieronim Jakubowskiantibody (Cell Signaling Technology) for 16 h. Images were captured using Western Bright Quantum-Advansta K12042-D20 and GeneGnome XRQ NPC chemiluminescence detection system. Band intensities were calculated using the

(9) Diet-induced hyperhomocysteinemia causes sex-dependent deficiencies in offspring musculature and brain functionFront Cell Dev BiolMarch 15, 2024Joanna Suszyńska-Zajczyk, Łukasz Witucki, Joanna Perła-Kaján, Hieronim Jakubowskihorseradish peroxidase (1 h, RT). Positive signals were detected using the WesternBright Quantum-Advansta K12042-D20 and GeneGnome XRQ NPC chemiluminescence detection system. Band intensity was calculated using the GeneTools

(10) Mitochondrial Dysfunction in Advanced Maternal Aged Cumulus Cells: A Possible Link to ATP Synthase Impairment?BiomoleculesFebruary 26, 2024Sandra Almeida-Reis, Alexandra Carvalho, Conceição Dias, Raquel Brito, Rita Silva, Teresa Almeida-Santos, João Ramalho-Santos, Ana Paula Sousaimmunoreactive bands were detected using Clarity Western ECL Substrate WesternBright TM Quantum (No. K-12042-D20; Advansta, San Jose, CA, USA) with ImagQuant LAS 500 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden).

(11) Iron regulatory proteins 1 and 2 have opposing roles in regulating inflammation in bacterial orchitisJCI InsightFebruary 1, 2024Niraj Ghatpande, Aileen Harrer, Bar Azoulay-Botzer, Noga Guttmann-Raviv, Sudhanshu Bhushan, Andreas Meinhardt, Esther G. Meyron-Holtztemperature. Antibody detection was performed using the ECL Plus chemiluminescence Western Blot kit (Advansta, K-12042-D20). Quantification of mRNA by quantitative RT-PCR. RNA was isolated from snap-frozen testes using TRIzol

(12) Deletion of the Homocysteine Thiolactone Detoxifying Enzyme Bleomycin Hydrolase, in Mice, Causes Memory and Neurological Deficits and Worsens Alzheimer’s Disease-Related Behavioral and Biochemical Traits in the 5xFAD Model of Alzheimer’s DiseaseJournal of Alzheimer's DiseaseOctober 10, 2023Łukasz Witucki, Kamila Borowczyk, Joanna Suszyńska-Zajczyk, Ewelina Warzych, Piotr Pawlak, Hieronim Jakubowskiconjugated with horseradish peroxidase. Positive signals were detected using Western Bright Quantum-Advansta K12042-D20 and GeneGnome XRQ NPC chemiluminescence detection system. Bands intensity was calculated using Gene

(13) Deletion of the Homocysteine Thiolactone Detoxifying Enzyme Bleomycin Hydrolase, in Mice, Causes Memory and Neurological Deficits and Worsens Alzheimer’s Disease-Related Behavioral and Biochemical Traits in the 5xFAD Model of Alzheimer’s DiseaseJ Alzheimers DisSeptember 12, 2023Łukasz Witucki, Kamila Borowczyk, Joanna Suszyńska-Zajczyk, Ewelina Warzych, Piotr Pawlak, Hieronim Jakubowskiconjugated with horseradish peroxidase. Positive signals were detected using Western Bright Quantum-Advansta K12042-D20 and GeneGnome XRQ NPC chemiluminescence detection system. Bands intensity was calculated using Gene

(14) RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replicationOpen biologyAugust 1, 2023Chang YC, Lin K, Baxley RM, Durrett W, Wang L, Stojkova O, Billmann M, Ward H, Myers CL, Bielinsky AK.times (10 min each) and detection was performed using WesternBright Quantum detection kit (Advansta no. K-12042-D20) and HyBlot ES high sensitivity autoradiography film (Thomas Scientific no. E3212). Primary antibodies

(15) Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewaliScienceJuly 21, 2023Yeh DW, Liu C, Hernandez JC, Tahara SM, Tsukamoto H, Machida K.,Thermo Fisher Scientific,4309155 WesternBright ECL-spray,Advansta,K-12049-D50 WesternBright ECL Quantum,Advansta,K-12042-D20 "Human Recombinant EZH2, GST tagged, full length",Creative BioMart,EZH2-285H Human Recombinant SUZ12

(16) Fanconi anemia-associated chromosomal radial formation is dependent on POLθ-mediated alternative end joiningCell ReportsMay 1, 2023Colette B. Rogers, Rachel E. Kram, Kevin Lin, Chad L. Myers, Alexandra Sobeck, Eric A. Hendrickson, Anja-Katrin BielinskyLipofectamine 3000 Transfection Reagent,ThermoFisher Scientific,Cat #L3000001 WesternBright Quantum HRP Substrate,Advansta,Cat # K-12042-D20 Annexin V Apoptosis Detection Kit,Biolegend,Cat # 640932 ,, Experimental models: Cell lines,Experimental models

(17) Deletion of the Transcriptional Coactivator HCF-1 In Vivo Impairs the Removal of Repressive Heterochromatin from Latent HSV Genomes and Suppresses the Initiation of Viral ReactivationmBioFebruary 28, 2023Jesse H. Arbuckle, Jodi L. Vogel, Stacey Efstathiou, Thomas M. KristieHCF-1 and control actin (Table S1), visualized with WesternBright Quantum (Advansta; catalog number K-12042-D20), and bands were quantitated using Syngene G:Box Chemi XT4 (Syngene; GeneTools 4.03.02.0v software). Chromatin

(18) Depletion of Paraoxonase 1 (Pon1) Dysregulates mTOR, Autophagy, and Accelerates Amyloid Beta Accumulation in MiceCellsFebruary 26, 2023Łukasz Witucki, Hieronim Jakubowskianti-rabbit IgG secondary antibody. Positive signals were detected using Western Bright Quantum-Advansta K12042-D20 and GeneGnome XRQ NPC chemiluminescence detection system. Signal intensity was assessed using the Gene

(19) Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography versus UltracentrifugationBiomoleculesFebruary 2, 2023Soares M, Pinto MM, Nobre RJ, de Almeida LP, da Graça Rasteiro M, Almeida-Santos T, Ramalho-Santos J, Sousa AP.immunoreactive bands were visualized using Clarity Western ECL Substrate WesternBright TM Quantum (K-12042-D20; Advansta, San Jose, CA, USA) with ImagQuant LAS 500 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden).

(20) Early Mitochondrial Defects in the 5xFAD Mouse Model of Alzheimer's DiseaseJournal of Alzheimer's disease : JADJanuary 1, 2023Sharma N, Banerjee R, Davis RL.were washed 3 times in 1X TBST buffer and proteins were visualized using the ECL reagent (Advansta, K-12042-D20) on a Chemidoc imager. ImageJ software was used to analyze the scanned blots and to quantify signal

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WesternBright Quantum HRP Substrate from Advansta

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Advansta

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WesternBright Quantum HRP Substrate from Advansta

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