WesternBright ECL HRP Substrate from Advansta

Product Specs
ItemWesternBright ECL HRP Substrate
CompanyAdvansta
Price $347.00Get Pricing
Catalog NumberK-12045-D50
Quantity500ml
ApplicationsWestern blotting

Specifications/Features

WesternBright® ECL is more sensitive than other chemiluminescent substrates used for routine Western blots imaged using film, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL. Features- Stronger signal than Amersham's ECL or Pierce's ECL. Detect low pg protein amounts. Use up to ten-times less antibody

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) MITF Regulates CFTR Expression to Participate in Myocardial Ischemia-Reperfusion InjuryJ Gene MedAugust 6, 2026Baoxin Tang, Chenying Zhu, Heqing Wang, Mingkui Gao, Tieyan Liroom temperature. Proteins were visualized using the ECL chemiluminescent substrate (ultrasensitive) (K‐12045‐D50, Advansta). 2.11 Cell Counting Kit 8 Assay AC16 cell viability was evaluated with a Cell Counting

(2) TRP120-dependent activation of noncanonical Wnt/NFAT signaling drives monocyte chemokine production in Ehrlichia chaffeensis infectionmSphereJuly 28, 2026Regina N. Solomon, Duc-Cuong Bui, Ayana P. Pai, Jere W. McBridesaline with 0.1% Tween 20 (TBST) and incubated with WesternBright Sirius Chemiluminescent Detection Kit (K-12045-D50; Advansta). Image acquisition was performed with the ChemiDoc MP Imaging System (Bio-Rad) followed by

(3) Tubal Patency Decoction and Its Ingredient β-Sitosterol Alleviate Tubal Infertility by Inhibiting Inflammation and Promoting Cilia Formation Through PGRMediators InflammJuly 27, 2026Liang Shao, Nansu Wang, Yan Yan, Qiongfang Tan, Yuying Huang, Qin Wu, Yali Tan, Yue Zhu, Yaqiong Xia, Ling Liutemperature for 1 h. The target bands were visualized by SuperECL Plus ultrasensitive luminescent solution (K‐12045‐D50, Advansta, USA) and imaged with a gel imaging system (ChemiScope6100, Clinx, China). GAPDH was used

(4) Expansion, functional diversification, and gene fusion events in the Ato protein familyiScienceJuly 17, 2026Faezeh Ghasemi, Patrícia Ataíde, Cláudia Barata-Antunes, Yiannis Pyrris, João Alves, Vitor Fernandes, Rosana Alves, Alexandra Gomes-Gonçalves, Margarida Casal, Wouter Van Genechten, et al.blue,Sigma-Aldrich,B0126-25G 2-mercaptoethanol,Sigma-Aldrich,M3148-25ML Ponceau S,Sigma-Aldrich,81460-5G WesternBright ECL HRP substrate kit,Advansta,K-12045-D50 bis-acrylamide (37.5:1),Fischer Scientific,BP14101-1 L [1-14C]-acetate,Perkin Elmer,NEC084A001MC L-[14C-(U)]-lactate,NEN

(5) An E3 Aptamer-Modified T Cell-Derived Exosomal Nanoplatform for Codelivery of Astragaloside IV and PESV: Enhancing Prostate Cancer Therapy Through Immune Modulation with Implications for Clinical TranslationInt J NanomedicineJuly 9, 2026Qing Zhang, Chenguang Liu, Sirui Wu, Nan Zhong, Junfeng Qiu, Wei Fu, Xujun YouProteintech, China) for 60 min at room temperature. Finally, freshly prepared ECL emitting solution (K-12045-D50, Advansta, USA) was added on the membrane for 2 min, the PVDF membrane was imaged and analyzed. The

(6) Combined All-trans Retinoic Acid and Rosiglitazone Alleviates Scleroderma Fibrosis by Suppressing the STAT3/Th17 AxisIn VivoJuly 1, 2026HONG-MEI SHAO, JUN-JIE CHEN, SHUANG-LIN CAO1:5,000; Proteintech). Protein bands were detected using an enhanced chemiluminescence (ECL) kit (Cat. No. K-12045-D50; Advansta, San Jose, CA, USA) and quantified by densitometric analysis with ImageJ software (v1.53;

(7) Yippee-like protein Moh1 links gene expression to metabolism and selective stress resistance in Saccharomyces cerevisiaeMicrob CellJune 29, 2026Çağla Ece Olgun, Gizem Turan Duman, Gizem Güpür, Hamit İzgi, Mariam Huda, Demet Çetin, Zekiye Suludere, Fatma Küçük Baloğlu, Ayşe Koca Çaydaşı, Mesut Muyanhour at room temperature. The membrane was then treated with WesternBright ECL substrate (Advansta, K-12045-D50) in a 1:1 ratio of luminol-enhancer reagent to peroxide reagent in the dark for 2 minutes to detect

(8) Dual SYK-HDAC Inhibitor Elicits Striking Efficacy against Acute Myeloid Leukemia: Rational Design, Synthesis, and Biological EvaluationJournal of Medicinal ChemistryJune 25, 2026Anshul Mishra, Wen-Bin Yang, Tsu-Shao Liu, Amandeep Thakur, Ajmer Singh Grewal, Jacek Marczyk, Giovanni Stelitano, Ram Sharma, Mandeep Rana, Gurpreet Singh, et al.antibodies. The chemiluminescent signals were developed with an Advansta WesternBright ECL HRP substrate (#K-12045-D20; San Jose, CA, USA) and acquired by an iBright FL1500 imaging system (Thermo Fischer Scientific Inc.).

(9) Alirocumab Plus Cemiplimab in Immunorefractory NSCLC: A Single Arm Phase 2 StudyResearch SquareJune 24, 2026Eziafa Oduah, Jhanelle Gray, Thomas Stinchcombe, Steven Wolf, Xiaodi Qin, Abbie Ireland, Joel Rivera-Concepcion, Jeffrey Clarke, Jeffrey Crawford, Laura Alder, et al.10 min at RT in TBS-T. Membranes were exposed to WesternBright HRP Quantum substrate (Advansta cat# K-12045-D50) and detected on Hyblot CL film (Denville Scientific Inc). Primary antibodies included: total AKT (1:1000,

(10) A Non-Channel Function of CFTR: Attenuating Mitochondrial Oxidative Stress and Cardiomyocyte Senescence via Stabilization by USP45Aging CellJune 23, 2026Chun Chen, Longtan Jiang, Yuewen Qiu, Chong Liu, Xiao Long, Pengcui Wu, Liang Li, Haixia Xu, Ping Deng, Li Yang, et al.temperature. The protein bands were visualized using SuperECL Plus ultrasensitive luminescence solution (K‐12045‐D50, Advansta) and imaged with a ChemiScope 6100 imaging system (Clinx, China). Band intensity (gray value)

(11) Integrin CD11b/CD18 reprograms macrophage polarization by suppressing ERK/STAT3 signaling to enhance antitumor immunity in colitis-associated colorectal cancerJ Immunother CancerJune 18, 2026Ying Lin, Yuan-Kun Liu, Peng Mi, Xian-Ling Zhao, Jun-Hui Lin, Xiao-Shen Cheng, Lai-Ying Liang, Yong-Dong Huang, Ya-Ni Huo, Gui-Jing Xie, et al.the protein bands were visualized using the ECL Chemiluminescence Detection Kit (Advansta, USA, Cat# K-12045-D50) and quantified with ImageJ software (RRID: SCR_003070). All antibodies used for western blotting are

(12) Potential Relationship Between YTHDF3 and CFTR in Myocardial Ischemia-Reperfusion InjuryJ Cell Mol MedJune 9, 2026Baoxin Tang, Chenying Zhu, Heqing Wang, Mingkui Gao, Tieyan Li1:5000), for 1 h at room temperature. Signals were developed using ECL chemiluminescent substrate (K‐12045‐D50, Advansta) and captured with a Chemiscope6100 imaging system (CLINX). Band intensity was measured using

(13) Compensatory respiration supports survival during extended heat stress in Microcystis aeruginosaSci AdvJune 5, 2026Oded Liran, Eli Shemesh, Dan Tchernov, Kevin J. Kunstman, Stefan J. Green, Surabhi Naik, Hagit Zer, Nir Keren, Assaf Sukenik, Reham Kh. KhalilAS06172), and cytochrome b6 (Agrisera, catalog no. AS184169). WesternBright ECL kit (Advansta, catalog no. K-12045-D50) was used as chemiluminescence substrate. The signal was captured using Gel Imager (ChemiDoc, Bio-Rad,

(14) Targeting fibroblast TXNDC5 resolves tumor desmoplasia and PD-1 resistance in colorectal cancer with mesenchymal traitsNature CommunicationsJune 5, 2026Kai-Lin Cheng, Chih-I Chen, Shu-Han Yu, Huai-Wen Liang, Yi-Wei Tsai, Chen-Ting Hung, Yu-Shan Lin, Gang-Hui Lee, Po-Yu Chen, Ming-Jer Tang, et al.#31460, 1:5000), washed with PBS-T, and developed using WesternBright ECL HRP substrate (Advansta, #K-12045-D50). Protein bands were imaged using the ChemiDoc MP system (BioRad Laboratories). The intensity of protein

(15) Reduced FBXO22 skews human trophoblast fate equilibrium toward syncytialization via polyubiquitinating the CoREST complexNucleic Acids ResJune 4, 2026Hongli Li, Guangmin Song, Linwei Zhou, Man Zhang, Yun Li, Xinmi Liu, Xing Wang, Li Yang, Xinyi Tao, Richard D Cannon, et al.chains (1:1000, Abmart). Protein bands were visualized using enhanced chemiluminescence (ECL) reagents (K-12045-D50, Advansta), detected with chemiluminescence imaging systems (Bio-Rad FusionFx), and quantified using

(16) Intestinal epithelial PIEZO1 regulates macrophage-to-myofibroblast transition via epithelial-derived TGF-α signaling in Crohn’s diseaseCell Communication and SignalingMay 28, 2026Jing Yang, Jinyan Jing, Ying Wang, Qing Zhao, Ni Ma, Danxia Liang, Zexiong Su, Yan Hu, Zhiling Li, Hanshi Zeng, et al.at room temperature. Proteins were detected using enhanced chemiluminescence (Advansta, USA; Cat. # K-12045-D50) and visualized with a chemiluminescence imaging system (Bio-Rad). Exposure times were carefully adjusted

(17) Mitochondrial NADH-redox inflexibility constrains genomic and epigenetic stability in pluripotent stem cellsThe EMBO JournalMay 27, 2026Tanveer Ahmed, Hui Zhang, Ghulam M Mushraf, Yongzhang Pan, Zeyu Zhang, Zhenzhu Sun, Mengran Yin, Xueting Xu, Xinyu Wu, Yin Gao, et al.temperature. The membrane was washed again, and signals were detected using Western Bright ECL (Advansta cat. K-12045-D50), visualized with the FUSION SOLO 4 M machine (Vilber Lourmat). Primary antibodies used were: anti-ACTIN

(18) Electroacupuncture alleviates osteoarthritis and is associated with activation of the Nrf2/HO-1 pathway and reduced ferroptosis-related changesFrontiers in Molecular BiosciencesMay 21, 2026Mei Zhao, Lingxing Ouyang, Junjie Ma, Huimeng Zhu, Shengfang Zhu, Xuan Zhou, Bingyao Huang, Jun YanIgG (115-035–003, JACKSON). Signals were visualized using an enhanced chemiluminescence substrate (K-12045-D50, Advansta) and analyzed with imaging software (Chemiscope 6100, CLINX). GAPDH was used as the internal

(19) Heat Processing Reduces IgE Binding but Not Basophil Sensitivity to Pea Proteins in Pea-Allergic ChildrenNutrientsMay 19, 2026Malgorzata Teodorowicz, Anja E. M. Janssen, Joyce Emons, Willemijn Lissenberg, Anouk Verstappen, Janneke Ruinemans-Koertsusing the WesternBright™ enhanced chemiluminescence (ECL) substrate (Advansta Inc., San Jose, CA, USA, K-12045-D20) according to the manufacturer’s instructions. Chemiluminescent signals were imaged using a Bio-Rad

(20) Vespakinin-M delineates an AMPK/mTOR-arginine-TCA cycle axis to act as an immunometabolic switch in post-stroke microgliaRedox BiolMay 12, 2026Dexiao Wang, Jingyu Zhang, Zhejun Zhuang, Qian Wang, Jie Li, Xue Wang, Kunkun Li, Yunyun Liu, Yanhui Cao, Lijuan Li, et al.USA) for 1 h at RT. Protein bands were visualized using enhanced chemiluminescence substrate (ECL; #K-12045-D50, Advansta, China) and imaged on an Azure C280 system. Band intensities were quantified using ImageJ

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WesternBright ECL HRP Substrate from Advansta

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Advansta

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WesternBright ECL HRP Substrate from Advansta

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