WesternBright ECL HRP Substrate from Advansta

Product Specs
ItemWesternBright ECL HRP Substrate
CompanyAdvansta
Price $187.00Get Pricing
Catalog NumberK-12045-D20
Quantity200ml
ApplicationsWestern blotting

Specifications/Features

WesternBright® ECL is more sensitive than other chemiluminescent substrates used for routine Western blots imaged using film, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL. Features- Stronger signal than Amersham's ECL or Pierce's ECL. Detect low pg protein amounts. Use up to ten-times less antibody

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) Ghrelin Alleviates Aging-Related Cognitive Impairment by Regulating Autophagy-Related Signaling via the SHBG/JNK1/Beclin1 Axis in MicrogliaAging CellSeptember 29, 2026Lixing Zhou, Ying Cheng, Wanyu Zhao, Xuelian Sun, Fengjuan Hu, Meiling Ge, Yan Zhang, Gongchang Zhang, Birong Dong, Xiaolei Liuantibodies. Immunoreactive bands were detected using an enhanced chemiluminescence (ECL) detection kit (K‐12045‐D50, Advansta, USA) and visualized with a chemiluminescence imaging system (ChemiScope 6100, Clinx Science,

(2) Proteomics in Ataxia Telangiectasia Fibroblasts Revealed Disease Hallmarks Recovered by ATM VariantsInt J Mol SciSeptember 11, 2026Anastasia Ricci, Federica Biancucci, Gianluca Morganti, Muhammad Junaid Iqbal, Francesca Monittola, Mauro Magnani, Rita Crinelli, Michele Menotta170-6515, 170-6516). Immunoreactive bands were recorded using enhanced chemiluminescence (Advansta K-12045-D50) acquisition by the ChemiDoc Touch Imaging System (Bio-Rad). The whole-lane normalization (WLN) strategy

(3) Comparative Analysis of SMN2 Splicing Activity and Protein Production Following In Vitro Treatment with the Generic Risdiplam Drug Vapromin® and the Reference Drug Evrysdi®BiomedicinesSeptember 4, 2026Olga Strizhakova, Andrei Pershin, Yana Bahareva, Aleksandr Kazarov, Ivan Lyagoskin, Evgenia Bocharova, Roman Anisimov, Yury Gladchenko, Natalia Kholod, Inessa Kirik, et al.visualized using an enhanced chemiluminescence (ECL) substrate for HRP (Advansta, San Jose, CA, USA K-12045-D20). Molecular weight marker—Biolabmix RAV-10 (Novosibirsk, Russia, PS-1050) 6.5 to 270 kDa (6.5, 16, 30,

(4) FKBP52 dictates the dual role of p53 in hepatocellular carcinoma: Mutation-specific prognostic stratification and therapeutic implicationsJournal of Biological ChemistrySeptember 1, 2026Jinfeng Wu, Chengyan Tang, Zewu Meng, Weihong Chen, Li Zhu, Xiang Li, Yaoyu Liu, Xing WangRRID:AB_330924, CST; #7074S, RRID:AB_2099233, CST), then developed with WesternBright ECL HRP substrate (K-12045-D50, Advansta). For Co-IP, cells were lysed in RIPA buffer containing protease and phosphatase inhibitors

(5) Protocol for propagating and inoculating rice grassy stunt virus in rice plants using brown planthoppersSTAR ProtocolsSeptember 1, 2026Ming Wu, Guoyi Yang, Shanshan Zhao, Shuai Zhang, Jianguo WuBiotech,Cat# F0202-100T 2×RealStar Fast SYBR qPCR mix,GenStar,Cat# A301 Western Bright ECL,Advansta,Cat# K-12045-D50 PAGE Gel kit,Solarbio,Cat# T8090 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(6) TCGA-based identification and functional validation of PBK in intrahepatic cholangiocarcinoma progression with involvement of AKT signalingTransl OncolAugust 13, 2026Xiangtian Shi, Lei Zhang, Tengqi Wang, Meng Wang, Chao Wang, Zhangxu Liu, Guixin Zhangantibodies (BL003A/BL001A, Biosharp). Finally, membranes were treated with ECL chemiluminescence reagent (K-12045-D50, Advansta), and target protein bands were visualized using a ChemiScope6100 imaging system (CLiNX).

(7) GNG12 overexpression inhibits triple-negative breast cancer cell proliferation, migration, and invasionSci RepAugust 11, 2026Biao-Feng Shan, Le Zhao, Tao Hu, Jing-Hao Xu, Tong-Xu Zeng, Jian-Ming Tang, Bo Yusecondary antibodies. Protein bands were detected using a luminol-based ECL substrate (Advansta, CA, USA, K-12045-D50) and imaged with a digital chemiluminescence platform. Band intensities were normalized to GAPDH levels,

(8) MITF Regulates CFTR Expression to Participate in Myocardial Ischemia-Reperfusion InjuryJ Gene MedAugust 6, 2026Baoxin Tang, Chenying Zhu, Heqing Wang, Mingkui Gao, Tieyan Liroom temperature. Proteins were visualized using the ECL chemiluminescent substrate (ultrasensitive) (K‐12045‐D50, Advansta). 2.11 Cell Counting Kit 8 Assay AC16 cell viability was evaluated with a Cell Counting

(9) An activity-modulated transport route across myelin (TRAM) for motor-driven organelle transfer in oligodendrocytesNature NeuroscienceJuly 31, 2026Katie J. Chapple, Tabitha R. F. Green, Kristen H. Schuster, Sarah Wirth, Yi-Hsin Chen, Ulrike C. Gerwig, Marie Louise Aicher, Yeonsu Kim, Lina Komarek, Angus Brown, et al.Technologies). Washing was repeated then the sample was incubated for 1 min in WesternBright ECL mix (K-12045-D20, Advansta). Immunoblots were scanned using the Intas ChemiCam system or using the Li-Cor C-DiGit blot scanner

(10) TRP120-dependent activation of noncanonical Wnt/NFAT signaling drives monocyte chemokine production in Ehrlichia chaffeensis infectionmSphereJuly 28, 2026Regina N. Solomon, Duc-Cuong Bui, Ayana P. Pai, Jere W. McBridesaline with 0.1% Tween 20 (TBST) and incubated with WesternBright Sirius Chemiluminescent Detection Kit (K-12045-D50; Advansta). Image acquisition was performed with the ChemiDoc MP Imaging System (Bio-Rad) followed by

(11) Tubal Patency Decoction and Its Ingredient β-Sitosterol Alleviate Tubal Infertility by Inhibiting Inflammation and Promoting Cilia Formation Through PGRMediators InflammJuly 27, 2026Liang Shao, Nansu Wang, Yan Yan, Qiongfang Tan, Yuying Huang, Qin Wu, Yali Tan, Yue Zhu, Yaqiong Xia, Ling Liutemperature for 1 h. The target bands were visualized by SuperECL Plus ultrasensitive luminescent solution (K‐12045‐D50, Advansta, USA) and imaged with a gel imaging system (ChemiScope6100, Clinx, China). GAPDH was used

(12) Expansion, functional diversification, and gene fusion events in the Ato protein familyiScienceJuly 17, 2026Faezeh Ghasemi, Patrícia Ataíde, Cláudia Barata-Antunes, Yiannis Pyrris, João Alves, Vitor Fernandes, Rosana Alves, Alexandra Gomes-Gonçalves, Margarida Casal, Wouter Van Genechten, et al.blue,Sigma-Aldrich,B0126-25G 2-mercaptoethanol,Sigma-Aldrich,M3148-25ML Ponceau S,Sigma-Aldrich,81460-5G WesternBright ECL HRP substrate kit,Advansta,K-12045-D50 bis-acrylamide (37.5:1),Fischer Scientific,BP14101-1 L [1-14C]-acetate,Perkin Elmer,NEC084A001MC L-[14C-(U)]-lactate,NEN

(13) RXRα suppression drives hepatic metabolic and immune dysfunction in sepsisEMBO Molecular MedicineJuly 16, 2026Matyas Jelinek, Jolien Vandewalle, Marah Heyerick, Tineke Vanderhaeghen, Céline Van Dender, Steven Timmermans, Madeleine Hellemans, Daria Fijalkowska, Hilde De Rooster, Martin Guilliams, et al.170-8891 SensiFast SYBR No-Rox Kit,GeC Biotech,Cat# CSA-01190 WesternBright ECL kit,Advansta,Cat# K-12045-D20 Free Fatty Acid Assay kit,Abnova,Cat# KA1667 Bio-Plex Pro Mouse Cytokine Assay,Bio-Rad Laboratories,Cat#

(14) An E3 Aptamer-Modified T Cell-Derived Exosomal Nanoplatform for Codelivery of Astragaloside IV and PESV: Enhancing Prostate Cancer Therapy Through Immune Modulation with Implications for Clinical TranslationInt J NanomedicineJuly 9, 2026Qing Zhang, Chenguang Liu, Sirui Wu, Nan Zhong, Junfeng Qiu, Wei Fu, Xujun YouProteintech, China) for 60 min at room temperature. Finally, freshly prepared ECL emitting solution (K-12045-D50, Advansta, USA) was added on the membrane for 2 min, the PVDF membrane was imaged and analyzed. The

(15) PARG inhibition reduces ssDNA levels and limits RPA loading upon replication fork collapseEMBO ReportsJuly 4, 2026Alexandra Mihuț, Debanjan Ghosh, Adrián Kószó, Dávid Szüts, Sarka Andrs Salajkova, Martin Andrs, Jana Dobrovolna, Roberta Fajka-Boja, Gyula Timinszky5-iodo-2’-deoxyuridine",Sigma-Aldrich,I7125 Biotin Azide,Click Chemistry Tool,CCT-1265-5 WesternBright ECL HRP substrate,Advansta,DV-K-12045-D50 SuperSignal™ West Pico PLUS Chemiluminescent Substrate,Thermo Scientific,34580 Software,Software,Software Alliance

(16) Iron overload induces ROS-mediated neural stem/progenitor cells apoptosis via mTOR pathwayBMC NeuroscienceJuly 2, 2026Fengchun Zhao, Wei Quan, Qian Zhang, Yi Yin, Yibin Jiang, Xuyang Zhang, Huanhuan Li, Chao Zhang, Lan Li, Shengli Hu, et al.Immunoreactive bands were visualized using ChemiDoc XRS+ system (Bio-Rad) with WesternBright ECL Kits (Advansta, K-12045-D20). Band density was analyzed with Image Lab software (Bio-Rad), and β-actin was used as the internal

(17) Combined All-trans Retinoic Acid and Rosiglitazone Alleviates Scleroderma Fibrosis by Suppressing the STAT3/Th17 AxisIn VivoJuly 1, 2026HONG-MEI SHAO, JUN-JIE CHEN, SHUANG-LIN CAO1:5,000; Proteintech). Protein bands were detected using an enhanced chemiluminescence (ECL) kit (Cat. No. K-12045-D50; Advansta, San Jose, CA, USA) and quantified by densitometric analysis with ImageJ software (v1.53;

(18) Vespakinin-M delineates an AMPK/mTOR-arginine-TCA cycle axis to act as an immunometabolic switch in post-stroke microgliaRedox biologyJuly 1, 2026Wang D, Zhang J, Zhuang Z, Wang Q, Li J, Wang X, Li K, Liu Y, Cao Y, Li L, et al.USA) for 1 h at RT. Protein bands were visualized using enhanced chemiluminescence substrate (ECL; #K-12045-D50, Advansta, China) and imaged on an Azure C280 system. Band intensities were quantified using ImageJ

(19) Yippee-like protein Moh1 links gene expression to metabolism and selective stress resistance in Saccharomyces cerevisiaeMicrob CellJune 29, 2026Çağla Ece Olgun, Gizem Turan Duman, Gizem Güpür, Hamit İzgi, Mariam Huda, Demet Çetin, Zekiye Suludere, Fatma Küçük Baloğlu, Ayşe Koca Çaydaşı, Mesut Muyanhour at room temperature. The membrane was then treated with WesternBright ECL substrate (Advansta, K-12045-D50) in a 1:1 ratio of luminol-enhancer reagent to peroxide reagent in the dark for 2 minutes to detect

(20) BTN3A1 regulate the function of Vγ9Vδ2 T cells through TCR signaling pathway in ovarian cancerJournal of Ovarian ResearchJune 26, 2026Jian Liu, Yuqin Wang, Min Wu, Hui Wangantibodies for 1 h at 25 °C. Protein expression levels were observed using Western Bright ECL HRP substrate (K-12045-D50, Advansta) and a chemiluminescence imaging system (Bio-Rad). Colony formation A total of 250/500 cells

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WesternBright ECL HRP Substrate from Advansta

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Advansta

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WesternBright ECL HRP Substrate from Advansta

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