WesternBright ECL HRP Substrate from Advansta

Product Specs
ItemWesternBright ECL HRP Substrate
CompanyAdvansta
Price $187.00Get Pricing
Catalog NumberK-12045-D20
Quantity200ml
ApplicationsWestern blotting

Specifications/Features

WesternBright® ECL is more sensitive than other chemiluminescent substrates used for routine Western blots imaged using film, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL. Features- Stronger signal than Amersham's ECL or Pierce's ECL. Detect low pg protein amounts. Use up to ten-times less antibody

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) Lysine butyrylation modulates central carbon metabolism and photosynthesis in cyanobacteriaJournal of Biological ChemistryNovember 1, 2026Sihan Li, Mingkun Yang, Jian Lin, Yanyan Chen, Li Yuan, Jiao Zhan, Feng Gechemiluminescence was enhanced using an immunoblotting assay kit (Advansta, Menlo Park, CA, catalog no.: K-12045-D50) and detected using a scanner (ImageQuant, GE Healthcare). Quantification analysis was carried out using

(2) Induced-fit anti-GFRAL antibody ameliorates cancer cachexia and attenuates sympathetic outflow in tumor-bearing miceSci AdvOctober 30, 2026Sohyun Kim, Min Hee Lee, Jinsook Ahn, Sang-Hyeon Ju, Dong Sun Lee, Seohyun Choi, Kwang Wook Min, Minho Shong, Ho Min KimCell Signaling Technology) for 2 hours at room temperature, and blots were visualized using enhanced chemiluminescence (#K-12045-D20, Advansta). Images were acquired using an ODYSSEY instrument and analyzed using the Image Studio software

(3) Curcumin-Induced Vacuolizing Stress Reveals a Vulnerability Associated with NF1 Loss in Schwann CellsCellsOctober 20, 2026Assunta Maria Iazzetta, Francesca Sardina, Cinzia Rinaldoby Life Technologies. Bound antibodies were detected by chemiluminescence using WesternBright ECL (#K12045D50 Advansta, San Jose, CA, USA), and band intensities were quantified by densitometry with Image Lab software

(4) GSK3 maintains NSP12 stability to promote bat-origin swine acute diarrhea syndrome coronavirus infectionJournal of VirologyOctober 20, 2026Maowen Sun, Cong Yuan, Yueyue Duan, Lei Shi, Yu Zhang, Luyao Li, Wenquan Mao, Haixue Zheng, Qi WangProtein signals were measured by incubating the NC membrane with the WesternBright ECL kit (Advansta, K-12045-D50). Co-immunoprecipitation assay HEK293T cells were transfected with the indicated plasmids, and cell

(5) Loss of SREBF1 disrupts lipid signaling and causes slow muscle fiber degenerationiScienceOctober 16, 2026Rajashekar Donaka, Kamal Awad, Iryna Khrystoforova, Bassem Hezkial, Marco Brotto, David KarasikCocktail, EDTA-Free (100X)",Sigma-Aldrich,Cat#87785 WesternBright ECL- HRP Substarte,Advansta Inc,Cat#K-12045-D20 Trizole,Sigma-Aldrich,Cat#10296010 "Direct-zol™ RNA Miniprep Plus kit, Tustin",Zymo Research,Cat#R-2052 Lugol

(6) Skin-Whitening and Anti-Photoaging Activities of Limosilactobacillus reuteri DS0333 Culture Supernatant Produced in Unripe Rubus occidentalis L. Extract-Supplemented MediumJ Microbiol BiotechnolOctober 8, 2026Nu Ri Song, Seo Yeon Shin, Jae Hee Byun, Seon Ju Kim, Hye Young Kim, Won Seok Kim, Kyung Mok Parkin Table 3. Enhanced chemiluminescence detection reagent was obtained from Advansta (USA; Cat. No. K-12045-D50), and protein concentrations were determined using a Bradford assay reagent obtained from Bio-Rad (Bio-Rad

(7) Herpes simplex virus type 2 induces S-phase entry and arrest to regulate the cell cycle for efficient replicationJ Gen VirolOctober 7, 2026Huimin Wang, Ranqing Cheng, Miaomiao Li, Yuncheng Li, Siyu He, Longchao Xu, Yuhao Chen, Zhenhua Huang, Binlian Sun, Yalan Liu, et al.visualized using the ChemiScope System (Clinx, Shanghai, China) with an enhanced chemiluminescent substrate (K-12045-D50; Advansta, CA, USA). β-actin was used as a loading control. Protein molecular weight marker was from

(8) Combined Effects of ATRA and ASA on HeLa Cell Viability, MAPK/AKT-Related Protein Abundance, and Intracellular VEGF: An Integrated In Vitro and In Silico StudyPharmaceuticals (Basel)October 1, 2026Selim Afşar, Şeyma Dümür, Gazi Güner, Serkan Sarıkaya, Figen Efe Çamili, Gürhan Güney, Mine İslimye Taşkın, Dilek Düzgün Ergun, Öznur Dündar Akın, Çiğdem Usul Afşar, et al.Protein bands were visualized using WesternBright ECL substrate (Advansta, San Jose, CA, USA; Cat. No. K-12045-D50) and detected with the G:BOX Chemi XRQ imaging system (Syngene, Cambridge, UK). Densitometric analysis

(9) Ghrelin Alleviates Aging-Related Cognitive Impairment by Regulating Autophagy-Related Signaling via the SHBG/JNK1/Beclin1 Axis in MicrogliaAging CellSeptember 29, 2026Lixing Zhou, Ying Cheng, Wanyu Zhao, Xuelian Sun, Fengjuan Hu, Meiling Ge, Yan Zhang, Gongchang Zhang, Birong Dong, Xiaolei Liuantibodies. Immunoreactive bands were detected using an enhanced chemiluminescence (ECL) detection kit (K‐12045‐D50, Advansta, USA) and visualized with a chemiluminescence imaging system (ChemiScope 6100, Clinx Science,

(10) ATG4B-dependent processing of tandem-domain BmLC3 exposes a Tyr136-regulated C-terminal fragmentFrontiers in Cell and Developmental BiologySeptember 29, 2026Won-Dong Shin, Hun-Joo Kim, Sang-Hee Ma, Sang-Won Park, Yong-Woo Jun, Do-Kyung Lee, In-Young Hwang, Deok-Jin Jang, Jin-A Leeperoxidase-conjugated secondary antibodies for 1 h, and signals were detected using WesternBright ECL (Advansta, K-12045-D50). Primary antibodies were anti-GFP (Santa Cruz Biotechnology, sc-9996; 1:2,000), anti-beta-actin (Santa Cruz Biotechnology

(11) Proteomics in Ataxia Telangiectasia Fibroblasts Revealed Disease Hallmarks Recovered by ATM VariantsInt J Mol SciSeptember 11, 2026Anastasia Ricci, Federica Biancucci, Gianluca Morganti, Muhammad Junaid Iqbal, Francesca Monittola, Mauro Magnani, Rita Crinelli, Michele Menotta170-6515, 170-6516). Immunoreactive bands were recorded using enhanced chemiluminescence (Advansta K-12045-D50) acquisition by the ChemiDoc Touch Imaging System (Bio-Rad). The whole-lane normalization (WLN) strategy

(12) Comparative Analysis of SMN2 Splicing Activity and Protein Production Following In Vitro Treatment with the Generic Risdiplam Drug Vapromin® and the Reference Drug Evrysdi®BiomedicinesSeptember 4, 2026Olga Strizhakova, Andrei Pershin, Yana Bahareva, Aleksandr Kazarov, Ivan Lyagoskin, Evgenia Bocharova, Roman Anisimov, Yury Gladchenko, Natalia Kholod, Inessa Kirik, et al.visualized using an enhanced chemiluminescence (ECL) substrate for HRP (Advansta, San Jose, CA, USA K-12045-D20). Molecular weight marker—Biolabmix RAV-10 (Novosibirsk, Russia, PS-1050) 6.5 to 270 kDa (6.5, 16, 30,

(13) FKBP52 dictates the dual role of p53 in hepatocellular carcinoma: Mutation-specific prognostic stratification and therapeutic implicationsJournal of Biological ChemistrySeptember 1, 2026Jinfeng Wu, Chengyan Tang, Zewu Meng, Weihong Chen, Li Zhu, Xiang Li, Yaoyu Liu, Xing WangRRID:AB_330924, CST; #7074S, RRID:AB_2099233, CST), then developed with WesternBright ECL HRP substrate (K-12045-D50, Advansta). For Co-IP, cells were lysed in RIPA buffer containing protease and phosphatase inhibitors

(14) Protocol for propagating and inoculating rice grassy stunt virus in rice plants using brown planthoppersSTAR ProtocolsSeptember 1, 2026Ming Wu, Guoyi Yang, Shanshan Zhao, Shuai Zhang, Jianguo WuBiotech,Cat# F0202-100T 2×RealStar Fast SYBR qPCR mix,GenStar,Cat# A301 Western Bright ECL,Advansta,Cat# K-12045-D50 PAGE Gel kit,Solarbio,Cat# T8090 ,, Experimental models: Organisms/strains,Experimental models: Organisms/strains,Experimental models

(15) TCGA-based identification and functional validation of PBK in intrahepatic cholangiocarcinoma progression with involvement of AKT signalingTransl OncolAugust 13, 2026Xiangtian Shi, Lei Zhang, Tengqi Wang, Meng Wang, Chao Wang, Zhangxu Liu, Guixin Zhangantibodies (BL003A/BL001A, Biosharp). Finally, membranes were treated with ECL chemiluminescence reagent (K-12045-D50, Advansta), and target protein bands were visualized using a ChemiScope6100 imaging system (CLiNX).

(16) GNG12 overexpression inhibits triple-negative breast cancer cell proliferation, migration, and invasionSci RepAugust 11, 2026Biao-Feng Shan, Le Zhao, Tao Hu, Jing-Hao Xu, Tong-Xu Zeng, Jian-Ming Tang, Bo Yusecondary antibodies. Protein bands were detected using a luminol-based ECL substrate (Advansta, CA, USA, K-12045-D50) and imaged with a digital chemiluminescence platform. Band intensities were normalized to GAPDH levels,

(17) MITF Regulates CFTR Expression to Participate in Myocardial Ischemia-Reperfusion InjuryJ Gene MedAugust 6, 2026Baoxin Tang, Chenying Zhu, Heqing Wang, Mingkui Gao, Tieyan Liroom temperature. Proteins were visualized using the ECL chemiluminescent substrate (ultrasensitive) (K‐12045‐D50, Advansta). 2.11 Cell Counting Kit 8 Assay AC16 cell viability was evaluated with a Cell Counting

(18) An activity-modulated transport route across myelin (TRAM) for motor-driven organelle transfer in oligodendrocytesNature NeuroscienceJuly 31, 2026Katie J. Chapple, Tabitha R. F. Green, Kristen H. Schuster, Sarah Wirth, Yi-Hsin Chen, Ulrike C. Gerwig, Marie Louise Aicher, Yeonsu Kim, Lina Komarek, Angus Brown, et al.Technologies). Washing was repeated then the sample was incubated for 1 min in WesternBright ECL mix (K-12045-D20, Advansta). Immunoblots were scanned using the Intas ChemiCam system or using the Li-Cor C-DiGit blot scanner

(19) TRP120-dependent activation of noncanonical Wnt/NFAT signaling drives monocyte chemokine production in Ehrlichia chaffeensis infectionmSphereJuly 28, 2026Regina N. Solomon, Duc-Cuong Bui, Ayana P. Pai, Jere W. McBridesaline with 0.1% Tween 20 (TBST) and incubated with WesternBright Sirius Chemiluminescent Detection Kit (K-12045-D50; Advansta). Image acquisition was performed with the ChemiDoc MP Imaging System (Bio-Rad) followed by

(20) Tubal Patency Decoction and Its Ingredient β-Sitosterol Alleviate Tubal Infertility by Inhibiting Inflammation and Promoting Cilia Formation Through PGRMediators InflammJuly 27, 2026Liang Shao, Nansu Wang, Yan Yan, Qiongfang Tan, Yuying Huang, Qin Wu, Yali Tan, Yue Zhu, Yaqiong Xia, Ling Liutemperature for 1 h. The target bands were visualized by SuperECL Plus ultrasensitive luminescent solution (K‐12045‐D50, Advansta, USA) and imaged with a gel imaging system (ChemiScope6100, Clinx, China). GAPDH was used

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WesternBright ECL HRP Substrate from Advansta

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Advansta

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WesternBright ECL HRP Substrate from Advansta

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