WesternBright ECL HRP Substrate from Advansta

Product Specs
ItemWesternBright ECL HRP Substrate
CompanyAdvansta
Price $33.00Get Pricing
Catalog NumberK-12045-C20
Quantity20ml
ApplicationsWestern blotting

Specifications/Features

WesternBright® ECL is more sensitive than other chemiluminescent substrates used for routine Western blots imaged using film, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody. Save time and money with WesternBright ECL. Features- Stronger signal than Amersham's ECL or Pierce's ECL. Detect low pg protein amounts. Use up to ten-times less antibody

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) Integrated spatial multi-omics delineates fatty acid degradation fuels malignant evolution at the tumour periphery in cervical squamous cell carcinomaeBioMedicineMay 1, 2026Shitong Lin, Zhihui Luo, Yuting Li, Bai Hu, Minghan Qu, Binghan Liu, Canhui Cao, Ting Peng, Miaochun Xu, Yashi Xu, et al.temperature). After TBST washes, signals were detected using Western Bright ECL substrate (Advansta, #K-12045-C20). Detailed information of the antibodies used for WB was as follows: E-cadherin (Cell Signalling Technology,

(2) Quercetin-loaded exosomes derived from human umbilical cord mesenchymal stem cells alleviate microglia-mediated neuroinflammation via an anti-inflammatory mechanismInflammopharmacologyApril 21, 2026Sajeda Osman, Gulen Melike Demirbolat, Sevim Isikroom temperature. The membranes were then incubated in WesternBright ECL-HRP substrate (Advansta, ADV-K-12045-C20) for 1–2 min and visualized using a C-Digit Blot Scanner (LICOR). Membrane stripping was conducted to

(3) Comparative Analysis of Red Onion-Derived Exosome-Like Nanovesicles and Extract Reveals Sustained Immunomodulatory Effects in LPS/IFN-γ-Stimulated MicrogliaMol NeurobiolMarch 30, 2026Sedra Alhelwani, Nurdan Sena Degirmenci, Cihan Mehmet Altıntaş, Mehtap Aydın, Fikrettin Şahin, Mehmet Şenel, Sevim Işıkwith horseradish peroxidase (HRP) (Abcam, ab97080). Western Bright ECL-HRP substrate (Advansta, ADV-K-12045-C20) was used for the chemiluminescence reaction, and the membranes were visualized on the C-Digit Blot Scanner

(4) BRD4 regulates m6A of ESPL1 mRNA via interaction with ALKBH5 to modulate breast cancer progressionActa Pharmaceutica Sinica BMarch 1, 2025Haisheng Zhang, Linlin Lu, Cheng Yi, Tao Jiang, Yunqing Lu, Xianyuan Yang, Ke Zhong, Jiawang Zhou, Jiexin Li, Guoyou Xie, et al.(Sigma, WH0009700M1). Specific immune complexes were detected using Western Bright ECL Reagent (Cat# K-12045-C20, Advansta). The quantitative analysis of Western blot analysis was performed according to the previous

(5) ATM-dependent Phosphorylation of Nemo SQ Motifs Is Dispensable for Nemo-mediated Gene Expression Changes in Response to DNA Double-Strand BreaksThe Journal of ImmunologySeptember 1, 2024Rebecca A. Glynn, Katharina E. Hayer, Craig H. BassingHRP-conjugated secondary antibodies. The blots were visualized using WesternBright ECL HRP Substrate (Advansta, K-12045-C20) on the GBox Chemi XRQ (Syngene). RNA Sequencing RNA extraction, RNA library preparations, sequencing

(6) LOXL2 Expression Status Is Correlated With Molecular Characterizations of Cervical Carcinoma and Associated With Poor Cancer Survival via Epithelial-Mesenchymal Transition (EMT) PhenotypeFrontiers in oncologyJanuary 1, 2020Cao C, Lin S, Zhi W, Lazare C, Meng Y, Wu P, Gao P, Wei J, Wu P.ABclonal, 1:1000) at 37°C for 1 h, the PVDF membrane was performed using western bright ECL HRP substrate (K-12045-C20, Advansta) and analyzed by Image Lab (v4.1). RNA Extraction, PCR, and qRT-PCR The total RNA of cells

(7) Bio-fabrication of pigment-capped silver nanoparticles encountering antibiotic-resistant strains and their cytotoxic effect towards human epidermoid larynx carcinoma (HEp-2) cellsRSC AdvancesMay 20, 2019Lakshmipathy Muthukrishnan, Muralidharan Chellappa, Anima Nanda, Sudhakar Thukkaram, Gracyfathima Selvaraj, Bavanilatha Muthiah, Suresh Sagadevan, J. Anita Lett3% of BSA, probed with specific antibodies and developed using the ECL Blotting Substrate (cat. no. K-12045-C20, Advansta, USA). In the western blotting analysis, the following primary antibodies were used: rabbit

(8) Melatonin protects mouse granulosa cells against oxidative damage by inhibiting FOXO1-mediated autophagy: Implication of an antioxidation-independent mechanismRedox biologySeptember 1, 2018Shen M, Cao Y, Jiang Y, Wei Y, Liu H.(Cell Signaling Technology, 7076). Immunoreactive bands were detected using a WesternBright ECL HRP substrate kit (Advansta, K-12045-C20) according to the manufacturer's instructions. The relative expression of target proteins was normalized

(9) Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cellsMolecular medicine reportsJuly 1, 2017Shen M, Zhou L, Zhou P, Zhou W, Lin X.temperature for 2 h. Immunoreactivity was visualized using an Enhanced Chemiluminescence reagent (cat. no. K‑12045‑C20; Advansta, Inc., CA, USA). Densitometric analysis was performed using Quantity One software version

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(1)

5/10/2017WesternBright ECL HRP SubstrateDr. Elena DeryuginaWesternBright ECL HRP substrate from Advansta was used to detect by western blotting a specific protein in comparison with Thermo Scientific West Pico reagent used conventionally in our lab.
(1)

1/14/2015Evaluation of WesternBright ECL sample pack Lakshmi BolluI came to know about this product at the Annual Meeting of the American Association of Cancer Research, 2014, San Diego. The sample pack gave us pretty good Western blot results and even better ... Read Review

WesternBright ECL HRP Substrate from Advansta

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Specifications/Features

Advansta

Citations (9)

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WesternBright ECL HRP Substrate from Advansta

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