WesternBright Quantum HRP Substrate from Advansta

Product Specs
ItemWesternBright Quantum HRP Substrate
CompanyAdvansta
Price $263.00Supplier Page
Catalog NumberK-12042-D10
Quantity100ml
ApplicationsWestern blotting, ELISA

Specifications/Features

WesternBright® Quantum® is formulated to take full advantage of the linear dynamic range of CCD imagers. It provides high sensitivity to detect low abundance proteins, and does not exhibit substrate depletion with high abundance proteins, so has a larger linear dynamic range than other commercially available substrates. A signal linear with respect to protein concentration over 3 orders of magnitude provides the ability to accurately quantify and compare protein amounts in your samples. Features- Sensitive - detect attomoles of protein per band. Quantitative - linear range of signal with respect to protein amount exceeds 3 orders of magnitude. Low background - for high signal to noise. Long lasting signal - image blots hours after substrate incubation. Versatile - Optimized for CCD imaging, and compatible with film detection

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) NCKAP1 coordinates focal adhesion and adherens junction dynamics in endothelial barrier maintenance and angiogenesisiScienceMay 15, 2026Yunyun Xiao, Paul-Lennard Mendez, Christian Hiepen, Jerome Jatzlau, Petra Knausassays,Critical commercial assays,Critical commercial assays ,, Western Bright Quantum kit,Advansta,Cat#K-12042-D10 NucleoSpin RNA isolation kit,Macherey-Nagel,Cat#740955.250 Duolink In Situ PLA® Probe Anti-Mouse MINUS,Sigma-Aldrich,Cat#DUO92004 Duolink In Situ PLA® Probe Anti-Rabbit PLUS

(2) L-Phenylalanine is a metabolic checkpoint of human Th2 cellsCell Reports MedicineDecember 16, 2025Abhijeet J. Kulkarni, Juan Rodriguez-Coira, Nino Stocker, Urszula Radzikowska, Antonio J. García-Cívico, María Isabel Delgado Dolset, Nuria Contreras, Inés Jardón Parages, Vanesa Saiz Sanchez, Pilar Serrano, et al.Precision Plus Protein Dual Color Standards,Bio-Rad Laboratories,Cat# #1610374 WesternBright Quantum - HRP Substrate,Advansta Corporation,Cat#K-12042-D10 Restore PLUS Western Blot Stripping buffer,Thermo Fisher Scientific,Cat#46430 KYT0353,Tocris Bioscience,Cat#5026 RIPA Lysis and Extraction buffer

(3) Auranofin synergizes with cisplatin in reducing tumor burden of Notch-dependent ovarian cancerCancer Research CommunicationsOctober 1, 2025Robert J. Lake, Parisa Nikeghbal, Irina V. Lagutina, Kimberly K. Leslie, Mara P. Steinkamp, Hua-Ying Faneither SuperSignal West Pico (#34083; Thermo Fisher Scientific) or WesternBright Quantum (Advansta, # K-12042-D10) horseradish peroxidase (HRP) chemiluminescent substrate and imaged using a Konica Processor SRX-101A. Antibodies Rabbit

(4) Auranofin Synergizes with Cisplatin in Reducing Tumor Burden of NOTCH-Dependent Ovarian CancerCancer Res CommunOctober 1, 2025Robert J. Lake, Parisa Nikeghbal, Irina V. Lagutina, Kimberly K. Leslie, Mara P. Steinkamp, Hua-Ying Faneither SuperSignal West Pico (#34083; Thermo Fisher Scientific) or WesternBright Quantum (Advansta, # K-12042-D10) horseradish peroxidase (HRP) chemiluminescent substrate and imaged using a Konica Processor SRX-101A. Antibodies Rabbit

(5) Lysine specific demethylase 1 conditional myeloid cell knockout mice have decreased osteoclast differentiation due to increased IFN-β gene expressionJBMR PlusDecember 3, 2024Kristina Astleford-Hopper, Juan E Abrahante Llorens, Elizabeth W Bradley, Kim C Manskywas incubated with membranes, washed, and developed using Western Bright Quantum (Advansta, catalog #K12042D10) detection agent. Images were acquired using BioRad Chemitouch. Immunoprecipitation Bone marrow cells

(6) Toxic small alarmone synthetase FaRel2 inhibits translation by pyrophosphorylating tRNA Gly and tRNA ThrScience AdvancesNovember 15, 2024Tatsuaki Kurata, Masaki Takegawa, Takayuki Ohira, Egor A. Syroegin, Gemma C. Atkinson, Marcus J.O. Johansson, Yury S. Polikanov, Abel Garcia-Pino, Tsutomu Suzuki, Vasili Hauryliuk(1:5000 dilution; Agrisera, AS111772) and PBS-T. ECL detection was performed using WesternBright Quantum (K-12042-D10, Advansta) Western blotting substrate and Amersham ImageQuant 800 imaging system (Cytiva). Structural

(7) Dynamic remodeling of septin structures fine-tunes myogenic differentiationiScienceSeptember 20, 2024Vladimir Ugorets, Paul-Lennard Mendez, Dmitrii Zagrebin, Giulia Russo, Yannic Kerkhoff, Georgios Kotsaris, Jerome Jatzlau, Sigmar Stricker, Petra Knausassays,Critical commercial assays,Critical commercial assays ,, WesternBright Quantum kit,Advansta,Cat# K-12042-D10 NucleoSpin RNA isolation kit,Macherey-Nagel,Cat# 740955.250 NEBuilder® HiFi DNA Assembly Cloning Kit,New England BioLabs

(8) A role for the S4-domain containing protein YlmH in ribosome-associated quality control in Bacillus subtilisNucleic Acids ResearchAugust 12, 2024Hiraku Takada, Helge Paternoga, Keigo Fujiwara, Jose A Nakamoto, Esther N Park, Lyudmila Dimitrova-Paternoga, Bertrand Beckert, Merilin Saarma, Tanel Tenson, Allen R Buskirk, et al.antibodies. Enhanced chemiluminescence (ECL) detection was performed using WesternBrightTM Quantum (K-12042-D10, Advansta) western blotting substrate using an ImageQuant LAS 4000 (GE Healthcare) imaging system. ApdA

(9) Kv3.4 regulates cell migration and invasion through TGF-β-induced epithelial-mesenchymal transition in A549 cellsScientific reportsJanuary 28, 2024Sim HJ, Kim MR, Song MS, Lee SY.(GenDEPOT, #SA001-500) over 2 h and visualized using a WesternBright™ Quantum™ (Advansta, California, USA, #K-12042-D10). An ImageQuant™ LAS 4000 image analyzer (GE Healthcare Life Sciences) was used to visualize immunocomplexes,

(10) Acid ceramidase regulates innate immune memoryCell reportsDecember 26, 2023Rother N, Yanginlar C, Prévot G, Jonkman I, Jacobs M, van Leent MMT, van Heck J, Matzaraki V, Azzun A, Morla-Folch J, et al.LDH CYTOTOXICITY ASSAY,Thermo Fisher Scientific,Cat#C20301 WESTERNBRIGHT QUANTUM KIT,Advansta,Cat#K-12042-D10 SLIDEFLASKS,Thermo Fisher Scientific,Cat#170920 RNEASY MINI KIT,Qiagen,Cat#74106 MAGNACHIP KIT,Merck-Millipore,Cat#17-408 KAPA RNA HYPERPREP KIT

(11) Shifted PAMs generate DNA overhangs and enhance SpCas9 post-catalytic complex dissociationNature Structural & Molecular BiologyNovember 1, 2023Jinglong Wang, Julien Le Gall, Richard L. Frock, Terence R. Strickanti-β-actin, 1:4,000, SC-47778, Santa Cruz Biotechnology) and horseradish peroxidase (HRP) substrate (K-12042-D10, Advansta) were used for the western blotting experiments. GFP knock-out and detection by flow cytometry The

(12) Identification of Molecular Mechanisms Responsible for the MMP-9-1562C/T Dependent Differential Regulation of Matrix Metalloproteinase-9 Expression in Human Neuron-like CellsGenes (Basel)October 31, 2023Sylwia Pabian-Jewuła, Magdalena Ambrożek-Latecka, Aneta Brągiel-Pieczonka, Klaudia Nowicka, Marcin Rylski4 °C. Proteins were detected by Western Bright Quantum HRP Substrate (Advansta, San Jose, CA, USA, #K-12042-D10), and chemiluminescence was measured using an Alliance apparatus (UVITEC, Cambridge, UK). 2.7. Gel Shift Assay

(13) Molecular fingerprints in the hippocampus of alcohol seeking during withdrawalResearch SquareSeptember 29, 2023Roberto Radwańska, Roberto Salamian, Ahmad Skonieczna, Bartosz Skonieczna, Bartosz Gielniewski, Bartek Gielniewski, Zofia Harda, Robbert Havekes, Robbert Havekes, Kasia Radwanskaagain. The membranes were visualized by G-Box apparatus using chemiluminescent reagent (Advansta, #K-12042-D10). TGX stain-free gels from Bio-Rad (#4568095) were used to standardize protein loading on gels [79]. Statistical

(14) Fluid shear stress-modulated chromatin accessibility reveals the mechano-dependency of endothelial SMAD1/5-mediated gene transcriptioniScienceSeptember 15, 2023Jerome Jatzlau, Paul-Lennard Mendez, Aybuge Altay, Lion Raaz, Yufei Zhang, Sophia Mähr, Akin Sesver, Maria Reichenbach, Stefan Mundlos, Martin Vingron, et al.assays ,, QuickExtract™ DNA Extraction Solution,Lucigen,Cat#QE09050 WesternBright Quantum kit,Advansta,Cat#K-12042-D10 NucleoSpin RNA XS isolation kit,Macherey-Nagel,Cat#740902.50 MinElute Reaction Cleanup Kit,Qiagen,Cat#28206 Tagment

(15) Molecular fingerprints in the hippocampus of alcohol seeking during withdrawalbioRxivSeptember 1, 2023Roberto Pagano, Ahmad Salamian, Edyta Skonieczna, Bartosz Wojtas, Bartek Gielniewski, Zofia Harda, Anna Cały, Robbert Havekes, Ted Abel, Kasia Radwanskaagain. The membranes were visualized by G-Box apparatus using chemiluminescent reagent (Advansta, #K-12042-D10). TGX stain-free gels from Bio-Rad (#4568095) were used to standardize protein loading on gels 79. Statistical

(16) TP53BP1, a dual-coding gene, uses promoter switching and translational reinitiation to express a smORF proteiniScienceMay 19, 2023Inchingolo MA, Diman A, Adamczewski M, Humphreys T, Jaquier-Gubler P, Curran JA.extracts,Promega,#L4380 Dual-Luciferase Reporter Assay System,Promega,#E1910 WesternBright™ Quantum,Advansta,#K-12042-D10 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, HEK293T,ATCC,CRL-3216 HeLa,ATCC,CCL-2 MCF-7,ATCC,HTB-22 MCF-10,ATCC,CRL-10317 THP-1

(17) Analysis of a rare progeria variant of Barrier-to-autointegration factor in Drosophila connects centromere function to tissue homeostasisCellular and Molecular Life SciencesMarch 1, 2023Tingting Duan, Srikantha Thyagarajan, Anastasia Amoiroglou, Gregory C. Rogers, Pamela K. Geyerand goat anti-rabbit 800, 1:10,00 LI-COR), and detected using WesternBright Quantum kit (Advansta, K-12042-D10). Purification of sfGFP-BAF variants from Drosophila S2 cells Coding sequences of the sfGFP-BAF and

(18) Failure to Thrive: Impaired BDNF Transport along the Cortical-Striatal Axis in Mouse Q140 Neurons of Huntington's DiseaseBiologyJanuary 19, 2023Maloney MT, Wang W, Bhowmick S, Millan I, Kapur M, Herrera N, Frost E, Zhang EY, Song S, Wang M, et al.with HRP-conjugated goat anti-rabbit or mouse second antibody and further with WesternBright™ Quantum (K-12042-D10, Advansta, San Jose, CA, USA). Exposed X-ray film was developed in a Kodak developer machine. β-actin

(19) DNA methylome and single-cell transcriptome analyses reveal CDA as a potential druggable target for ALK inhibitor–resistant lung cancer therapyExperimental & Molecular MedicineAugust 23, 2022Haejeong Heo et al.IgG (1:5000, 31460, Invitrogen). Immunopositive bands were detected using an ECL kit (K-12045-D50, K-12042-D10, Advansta, CA) and visualized using a Fujifilm LAS-4000 (Tokyo, Japan). siRNA-mediated gene knockdown Small

(20) Exacerbated VEGF up-regulation accompanies diabetes-aggravated hemorrhage in mice after experimental cerebral ischemia and delayed reperfusionNeural regeneration researchJuly 1, 2022Lai AKW, Ng TC, Hung VKL, Tam KC, Cheung CW, Chung SK, Lo ACY.detected using enhanced chemiluminescence reagents (RPN2106, GE Healthcare, Buckinghamshire, UK or K-12042-D10, Advansta, CA, USA) and light-sensitive films (47410 19291, Fujifilm, Tokyo, Japan), quantified using

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WesternBright Quantum HRP Substrate from Advansta

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Specifications/Features

Advansta

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WesternBright Quantum HRP Substrate from Advansta

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