Trans-Blot Turbo™ Transfer System from Bio-Rad

Product Specs
ItemTrans-Blot Turbo™ Transfer System
CompanyBio-Rad
Price Supplier Page
Catalog Number1704150
ApplicationsWestern Blot, Electrophoretic transfer
QuantityEA
FormatSemi-dry
CapacityCan transfer 1–4 mini or 1–2 midi gels in a single run

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Diethyl ether anesthesia inhibits phototropic response in Arabidopsis thaliana by disrupting auxin redistributionPlant Signal BehavDecember 31, 2026Martin Hřivňacký, Marek Rác, Tereza Miksteinová, Christian Luschnig, Andrej Pavlovičseparated in 6.5% (v/v) SDS-PAGE gel 77 and transferred to a nitrocellulose membrane (Bio-Rad, USA) in the Trans-Blot Turbo System (Bio-Rad, USA). Membranes were then stained with Ponceau-S to check the correct protein transfer.

(2) Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell linesInternational Journal of HyperthermiaDecember 31, 2026Samir V. Jenkins, Hayden Carlton, Bree Scott, Shelbie D. Stahr, Whit Vinson, Gabriel Fletcher, Azemat Jamshidi-Parsian, Ping-Ching Hsu, Ruud P. M. Dings, David Huitink, et al.SDS-PAGE using 4-20% Mini-PROTEAN TGX Stain-Free Gels. Proteins were transferred to PVDF membranes using Trans-Blot Turbo RTA mini Transfer System (all from Bio-Rad). Nonspecific sites in the membrane were blocked using Pierce

(3) Akkermansia muciniphila modulates intestinal mucus composition to counteract high-fat diet-induced obesity in miceGut MicrobesDecember 31, 2026Paola Paone, Camille Petitfils, Anthony Puel, Dimitris Latousakis, Willem M. de Vos, Nathalie M. Delzenne, Nathalie Juge, Matthias Van Hul, Patrice D. Canimin. The mucins/proteins were transferred onto Immobilon Psq (Merck) in Tris-glycine52 buffer, using Trans-blot Turbo (25 V, 1 A, 60 min; Biorad). The region of the blot were mucins migrated was cut out and the blot was

(4) ER proteostasis failure in HYOU1 deficiency alters B cells, neutrophils, and interferon signallingJ Hum ImmunNovember 2, 2026Aida Idani, Sabrina Bibi-Triki, Tristan Stemmelen, Mirjana Radosavljevic, Pierre Grenot, Aurélie Hirschler, Alice Bernard, Michelle Rosenzwajg, Jianying Yang, Alice Eischen, et al.NuSep), and subjected to electrophoresis at 150 V for 70 min. Protein transfer was performed using TransBlot Turbo Transfer System (Bio-Rad Laboratories) following the manufacturer’s instructions. Membranes were blocked

(5) 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regenerationBioactive MaterialsNovember 1, 2026Noemi Ravaglia, Arianna Rossi, Maurizio Vignolo, Pietro Galizia, Diana Pacheco, Federica Arienti, Carlo Baldisserri, Caitlin M. Guzzo, Monica Montesi, Rosa Mancinelli, et al.electrophoresis cell kit (Bio-Rad). Proteins were then transferred to nitrocellulose membranes by means of a Trans-Blot Turbo™ transfer system (Bio-Rad). The membranes were blocked for 40 min at room temperature in 5% nonfat dry milk diluted in

(6) ROS scavenging mitigates TNFα-induced endoplasmic reticulum stress and mitochondrial fragmentation in human airway smooth muscleFunction (Oxf)October 1, 2026Debanjali Dasgupta, Sanjana Mahadev Bhat, Gary C. Siecktransferred to a polyvinylidene difluoride (PVDF) membrane (0.2 μm; Bio-Rad, Hercules, CA) using the Bio-Rad Trans Blot Turbo system (RRID:SCR_023156; Hercules, CA). The Western blot membrane from the same experiment where samples,

(7) Proteomics reveal PTEN as a critical mediator of sustained mitochondrial dysfunction during chronic spinal cord injuryExperimental NeurologyOctober 1, 2026Samir P. Patel, Carlos A. Gartner, Mudjgiwa Patience, Jaydeepbhai Patel, Victoria K. Slone, Dylan E. Capes, Krithika Iyer, Maria F. Zapata-Jaramillo, Vamshi Kota, Michael T. Hash, et al.4-15% acrylamide SDS-PAGE gradient gel (4561086; Bio-Rad). Protein was transferred using the Bio-Rad Trans-Blot Turbo Semi-dry transfer system onto nitrocellulose membranes (1704158; Bio-Rad). Membranes were incubated

(8) Gfpt2 modulates fibroblast activation by glutathione metabolismJournal of Molecular and Cellular CardiologyOctober 1, 2026Paige Takasugi, Greg Farber, Luping Du, Shea N. Ricketts, Shay E. Rooney, Brian Spurlock, Haofei Wang, Jiandong Liu, Li Qianthen transferred to 0.2 μm nitrocellulose membranes with the Trans-Blot Turbo Transfer System (BioRad, 1704150) according to the manufacturer’s instructions. Blots were then blocked for 1 hour in 5% Bovine Serum

(9) Serine ubiquitination of the SARS-CoV-2 RdRp by the non-canonical E3 ligase MYCBP2mSphereSeptember 29, 2026RuthMabel Boytz, Jadon Layne, Cleopatra Skerrit-Genelus, Chengjin Ye, Jack Chun-Chieh Hsu, Luis Martinez-Sobrido, Maudry Laurent-Rolleexperiments, tris-glycine Mini-PROTEAN gels (BioRad) were used, and transfers were performed using the BioRad Trans-Blot Turbo transfer system. Membranes were blocked with TBST containing 5% bovine serum albumin (BSA) for 1 h at room temperature,

(10) Tissue-specific recombination-activated clonal and inducible transcription (TRAC-IT) enables controlled gene activation in stably marked tissuesPlant JSeptember 28, 2026Tristan Wijsman, Jana Wittmer, Robert Winter, Teun Bruins, Arjan van Zeijl, Harm Nijveen, Ben Scheres, Renze Heidstraaccording to the manufacturer protocol. Proteins were transferred to a PVDF membrane using the Bio‐Rad Trans‐Blot turbo system. The blot was blocked in PBST (8 g L−1 NaCl, 0.2 g L−1 KCl, 1.44 g L−1 Na2HPO4, 0.24 g L−1 KH2PO4,

(11) Activation of cGAS-STING axis by CO@OMVs promote macrophage mitophagy for CIRI treatmentAPL BioengSeptember 28, 2026Yiwei Chen, Shuntong Hu, Juan Huang, Pangyao Long, Jiahao Zhou, Longfeng Ran, Jinze Wu, Xiaobo Li, Huaizheng Liu, Yi Yuan, et al.The separated proteins were transferred onto PVDF membranes (Millipore, USA, Cat. #IPVH00010) using a Trans-Blot Turbo transfer system (Bio-Rad, USA). After transfer, membranes were blocked with 5% BSA (Sigma-Aldrich, USA, Cat. #A2153)

(12) Autophagy Flux Is Remodeled Sex- and Cell Type-Specifically During Human Aging, and Is Linked to Reduced Physical Function in Older AdultsAging CellSeptember 27, 2026Tatiana M. Moreno, Stephanie R. Heimler, Ryan J. Moran, Hava Shoshana Barkai, Lina Scandalis, Larissa Traxler, Ashley Neil, Stephen Dozier, Jaclyn Bergstrom, Alexandre R. Colas, et al.separated at 110 V. Proteins were transferred to a PVDF membrane (Bio‐Rad Laboratories, #1704156) using the Trans‐Blot Turbo system (Bio‐Rad Laboratories) at 1.3 A for 7 min. The membrane was cut horizontally at ~30 kDa. The

(13) High-Density Type I Collagen Promotes IFN-γ+ CD8+ T Cell Exhaustion via SAT1-ASS1-Mediated Glutamine Accumulation and Ferroptosis in Triple-Negative Breast CancerAdv Sci (Weinh)September 27, 2026Jinyan Wang, Shuangyue Pan, Lu Bai, Bin Li, Tiantian Liu, Jianing Cao, Mengdi Yang, Heda Zhang, Duancheng Guo, Wenxiang Zhi, et al.to Pierce polyvinylidene fluoride (PVDF) transfer membranes (88518, ThermoScientific, USA) using a Trans‐Blot Turbo Transfer System (BioRad). The PVDF membranes were blocked with 5% nonfat milk (P0216, Beyotime, China) supplemented

(14) Integrative synaptosome multi-omics reveals disrupted synapse organization and localized cryptic transcripts in C9ORF72-frontotemporal dementiaActa NeuropatholSeptember 25, 2026Ashton M. Spillman, Eric B. Alsop, Lauren M. Gittings, Krystine Garcia-Mansfield, Ignazio Piras, Anna Bonfitto, Melissa N. Martinez, Ritin Sharma, Kim R. Preller, Matthew Huentelman, et al.were run at 90 V for 30 min and switched to 200 V for 1 h. Protein transfer was conducted onto 0.2 µm Trans-Blot Turbo Nitrocellulose Membrane (BioRad, #12,023,955) using the Trans-Blot Turbo system (BioRad), following

(15) A designed ubiquitin-binding protein selectively enriches and visualizes unanchored ubiquitinSci AdvSeptember 25, 2026Shihua Shi, Alexandra Bendel, Georg Kempf, Jacint Sanchez, Sohei Majima, Vytautas Iesmantavicius, Daniel Hess, Jan Seebacher, Yong Huang, Sucheta Ghosh, et al.4 to 12% gradient Bis-Tris gels (200 V for 36 min), followed by membrane transfer using the Bio-Rad Trans-Blot Turbo transfer system with the 0.2-μm PVDF Transfer Packs (Bio-Rad, #1704156) using the “mixed molecular weight” program.

(16) SERPINB1 as a critical regulator of inflammation and protease activity in human monocytic cellsImmunohorizonsSeptember 24, 2026Jareb J Pérez-Caraballo, Xiang Ye, Jian Cui, Rubén Martínez-Barricarteby SDS-PAGE and transferred to a Polyvinylidene Difluoride (PDFV) membrane (Millipore-Sigma) using a Trans-Blot Turbo transfer system (Biorad). The membrane was probed with anti-SERPINB1 (generously provided by Eileen Remold-O’Donnell,

(17) A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal inhibitor that binds the peptidyl transferase centerCommun BiolSeptember 23, 2026Nino Schwaller, Dominic Andenmatten, Jonas Luginbühl, Julius Rabl, Helena Baur, Marc Chambon, Jonathan Vesin, Gerardo Turcatti, Evangelos D. Karousis(SIGMA-ALDRICH, Cat. No. E5134-500G), pH 7.3). Proteins were transferred onto nitrocellulose membranes using the Trans-Blot Turbo Transfer Pack (BIO-RAD, Cat. No. 1704158). Membranes were then cut according to molecular weight of

(18) Molecular mechanism of complement interference by Streptococcus pyogenes nuclease ACommun BiolSeptember 23, 2026Isabella A. Bennig, Joel Ströbaek, Rafael Mamede, Anton Sellerberg, Ariane Neumann, Ana Friães, Mario Ramirez, Michael Hall, Mattias Collin, Lars Malmström, et al.electrophoresed for 35 min at 150 V. Subsequently, proteins were transferred onto a 0.2 µm PVDF membrane (Trans-Blot Turbo Mini format, Bio-Rad Laboratories) using the Trans-Blot Turbo Transfer System for 5 min according to

(19) Enhancing the efficacy of B7-H3-targeting antibody-drug conjugate DS-7300a in TP53-deficient tumors by inducing ferroptosisOncogeneSeptember 22, 2026Javier Leo, Feiyu Chen, Wei Shi, Xin Liang, Chenling Meng, Qianlin Gu, Yaman Albittar, Zhen Fan, Jie Zhang, Boyi Gan, et al.TGX Precast Protein Gels (BioRad, 4561086) and transferred to the nitrocellulose membrane using the Trans-Blot Turbo RTA Mini 0.2-µm nitrocellulose transfer kit (Bio-Rad Laboratories, 1704270). The blots were then blocked

(20) Nuclear activities and interactome of the NS5 protein of tick-borne encephalitis virusJournal of VirologySeptember 22, 2026Maxime Chazal, Aïssatou Aïcha Sow, Elodie Le Seac'h, Dana Hawasheen, Margarida Bonifacio, Mikael Feracci, Ségolène Gracias, Adrià Sogues, Melissa Molho, Heng Pan, et al.NuPage 4%–12% Bis-Tris gels (Invitrogen) and transferred to nitrocellulose membranes (Bio-Rad) using a Trans-Blot Turbo Transfer system (Bio-Rad). Alternatively, high molecular weight proteins were separated by SDS-PAGE using NuPAGE 3-8%

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(1)

3/28/2025Fast and Reliable Protein Transfer System From Bio Rad for Western Bots Pavan Kumar ChodisettiWe utilized the Bio-Rad Trans-Blot® Turbo™ Transfer System to transfer proteins from SDS-PAGE gels to PVDF membranes for the immunodetection of cell wall hydrolases in Enterococcus faecium. Our ... Read Review
(1)

5/24/2023Old but Reliable Blot Transfer Equipment Manuel Jr VidalWe do Western blots on a regular basis. This equipment has always been the go-to equipment for our blot transfers.
(1)

7/6/2022Fast and Efficient Trans-Blot Turbo™ Transfer System Niroj ShresthaThe proteins from HEK293 cell lysate were resolved in NuPAGE 4%–12% Bis-Tris gel and transferred to 0.2-µm PVDF membrane for 7 minutes with the Trans-Blot Turbo Transfer System and kit as per ... Read Review

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