Trans-Blot Turbo™ Transfer System from Bio-Rad

Product Specs
ItemTrans-Blot Turbo™ Transfer System
CompanyBio-Rad
Price Supplier Page
Catalog Number1704150
ApplicationsWestern Blot, Electrophoretic transfer
QuantityEA
FormatSemi-dry
CapacityCan transfer 1–4 mini or 1–2 midi gels in a single run

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Akkermansia muciniphila modulates intestinal mucus composition to counteract high-fat diet-induced obesity in miceGut MicrobesDecember 31, 2026Paola Paone, Camille Petitfils, Anthony Puel, Dimitris Latousakis, Willem M. de Vos, Nathalie M. Delzenne, Nathalie Juge, Matthias Van Hul, Patrice D. Canimin. The mucins/proteins were transferred onto Immobilon Psq (Merck) in Tris-glycine52 buffer, using Trans-blot Turbo (25 V, 1 A, 60 min; Biorad). The region of the blot were mucins migrated was cut out and the blot was

(2) Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell linesInternational Journal of HyperthermiaDecember 31, 2026Samir V. Jenkins, Hayden Carlton, Bree Scott, Shelbie D. Stahr, Whit Vinson, Gabriel Fletcher, Azemat Jamshidi-Parsian, Ping-Ching Hsu, Ruud P. M. Dings, David Huitink, et al.SDS-PAGE using 4-20% Mini-PROTEAN TGX Stain-Free Gels. Proteins were transferred to PVDF membranes using Trans-Blot Turbo RTA mini Transfer System (all from Bio-Rad). Nonspecific sites in the membrane were blocked using Pierce

(3) Diethyl ether anesthesia inhibits phototropic response in Arabidopsis thaliana by disrupting auxin redistributionPlant Signal BehavDecember 31, 2026Martin Hřivňacký, Marek Rác, Tereza Miksteinová, Christian Luschnig, Andrej Pavlovičseparated in 6.5% (v/v) SDS-PAGE gel 77 and transferred to a nitrocellulose membrane (Bio-Rad, USA) in the Trans-Blot Turbo System (Bio-Rad, USA). Membranes were then stained with Ponceau-S to check the correct protein transfer.

(4) 4D piezoceramic-integrated scaffolds with bioelectric cues for skeletal muscle regenerationBioactive MaterialsNovember 1, 2026Noemi Ravaglia, Arianna Rossi, Maurizio Vignolo, Pietro Galizia, Diana Pacheco, Federica Arienti, Carlo Baldisserri, Caitlin M. Guzzo, Monica Montesi, Rosa Mancinelli, et al.electrophoresis cell kit (Bio-Rad). Proteins were then transferred to nitrocellulose membranes by means of a Trans-Blot Turbo™ transfer system (Bio-Rad). The membranes were blocked for 40 min at room temperature in 5% nonfat dry milk diluted in

(5) Proteomics reveal PTEN as a critical mediator of sustained mitochondrial dysfunction during chronic spinal cord injuryExperimental NeurologyOctober 1, 2026Samir P. Patel, Carlos A. Gartner, Mudjgiwa Patience, Jaydeepbhai Patel, Victoria K. Slone, Dylan E. Capes, Krithika Iyer, Maria F. Zapata-Jaramillo, Vamshi Kota, Michael T. Hash, et al.4-15% acrylamide SDS-PAGE gradient gel (4561086; Bio-Rad). Protein was transferred using the Bio-Rad Trans-Blot Turbo Semi-dry transfer system onto nitrocellulose membranes (1704158; Bio-Rad). Membranes were incubated

(6) CAR design equips diverse immune cells with unique antigen-specific activation identitiesMolecular Therapy OncologySeptember 17, 2026Ayano Sugiyama-Finnis, Takaharu Kimura, Kiyoko Izawa, Yuko Kawano, Takao Yogo, Hyojung Jeon, Tsuyoshi Fukushima, Ryoji Ito, Satoshi YamazakiElectroblotting of the gel onto polyvinylidene fluoride (PVDF) membranes was performed by the Bio-Rad Trans-Blot Turbo. PVDF membranes were blocked in 1x Tris-buffered saline (TBS) (Bio-Rad #1706435), 0.1% Tween 20 (Sigma-Aldrich

(7) AAVR disruption as a producer-cell engineering strategy for enhanced supernatant-based recovery of selected AAV serotypesMol Ther AdvSeptember 10, 2026Kenji Sakamoto, Kouhei Yoshida, Yuji Tsunekawa, Naymel A. Guzman Mendoza, Yui Komaki, Azusa Onodera, Takashi Okadabuffer) and transferred onto 0.45 μm PVDF membranes (Invitrogen) using a semi-dry transfer system (Trans-Blot Turbo, Bio-Rad). Tri-Glycine transfer buffer (Novex, Invitrogen) supplemented with 10% methanol was used for

(8) The potential of gene therapy to rescue telomeric and extratelomeric defects in X-linked dyskeratosis congenitaMol Ther AdvSeptember 10, 2026Diego Llorente-Arroyo, María Luz Lozano, Susana Navarro, Leandro Sastre, Rosario Perona, Juan Antonio Bueren, Guillermo Guenechea, Nestor Wenceslao MezaTGX Stain-Free gels (Bio-Rad) using a PowerPac HC high-current power supply (Bio-Rad) and transferred onto Trans-Blot Turbo Mini-size nitrocellulose membranes (Bio-Rad) using a Trans-Blot Turbo Transfer System (Bio-Rad). Membranes

(9) Development of an Antisense Oligonucleotide targeting EFEMP1 in models relevant to macular degenerationMolecular Therapy Nucleic AcidsSeptember 8, 2026William P. Miller, Said Arevalo-Alquichire, Paula Perez-Corredor, Rose Lin, Anil Upreti, Jeysson Sanchez-Suarez, Tim E. Vanderleest, Claudia Marino, Donovan Spencer, Matthew Scribner, et al.chemiluminescence, blots were transferred to PVDF using the trans-blot Turbo transfer system (Bio-Rad; 1704150) and trans-blot Turbo RTA Midi PVDF Transfer Kit (Bio-Rad, 1704273) according to the manufacturer’s

(10) Antisense Peptide Nucleic Acids Targeting Ribosomal Protein Genes: A Novel Strategy Against Carbapenem-resistant Acinetobacter baumanniiMolecular Therapy Nucleic AcidsSeptember 8, 2026Yoo Jeong Kim, Ju Hui Seo, Wook-Jong Jeon, Jee-woong Kim, Man Hwan Oh, Song-mee Bae, Dong Chan Chan MoonHercules, CA, USA). Proteins were then transferred onto polyvinylidene difluoride (PVDF) membranes (Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs, Bio-Rad Laboratories) using the Trans-Blot Turbo Transfer System (Bio-Rad). The

(11) Systemically delivered mRNA-LNPs transfect primary and secondary liver tumorsMolecular Therapy Nucleic AcidsSeptember 8, 2026Laura J. Leighton, Yee Jing Gee, Sachithrani U. Madugalle, Maria Victorova, Nissa L. Carrodus, Kim R. Bridle, Sidney A. Howell, Xiaowen Liang, Gregory C. Miller, Chris L.D. McMillan, et al.was transferred onto low-fluorescence polyvinylidene fluoride (PVDF) membranes (Bio-Rad) using the Trans-Blot Turbo (Bio-Rad) with the “1X Mini-TGX” preset (2.5 A and 25 V for 3 min). Membranes were blocked in EveryBlot blocking buffer

(12) PARP1 rewires neuroinflammatory and redox metabolism associated with reactive neuroglia in neuropathic painRedox BiologySeptember 1, 2026Simona Denaro, Simona D'Aprile, Anna Gervasi, Vincenzo Russo, Francesco Bellia, Sebastiano Giallongo, Alessandro Lavoro, Saverio Candido, Alice Braga, Alexander V. Gourine, et al.Bio-Rad) and then transferred to nitrocellulose membranes via a Trans-Blot Turbo Transfer System (Cat.no. 1704150, Bio-Rad). The membranes were incubated for 1 h at room temperature with blocking buffer consisting

(13) Molecular mechanisms underlying FCCP-induced mitophagy in human airway smooth muscle cellsAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologySeptember 1, 2026Sanjana Mahadev Mahadev Bhat, Oscar A. Ramirez Ramirez Ramirez, Gary C. Siecktransferred to a polyvinylidene difluoride (PVDF) membrane (0.2 μm; Bio-Rad, Hercules, CA) using the Bio-Rad Trans Blot Turbo system (RRID:SCR_023156; Hercules, CA). Following transfer, the membranes were blocked using EveryBlot blocking buffer

(14) Redox imbalance dictates dependence on GOT1 versus GOT2 for rod photoreceptor health during aging and stressRedox BiologySeptember 1, 2026Meini Chen, Eric Weh, Moloy T. Goswami, Katherine M. Weh, Heather Hager, Peter Sajjakulnukit, Avi J. Weingarten, Shubha Subramanya, Nicholas Miller, Sraboni Chaudhury, et al.Cat #5671094) and transferred to a PVDF membrane using the TurboBlot transfer system (Bio-Rad, Cat #1704150). After transfer, blots were incubated in 5% non-fat milk in TBS (Tris-buffered Saline, Bio-Rad, Cat

(15) Endothelial-Derived Extracellular Vesicles Impair Human Pulmonary Microvascular Cell Function in an In-Vitro Model of Sepsis-Induced Acute Lung InjuryAmerican Journal of Physiology-Lung Cellular and Molecular PhysiologySeptember 1, 2026Maya Cohen, Liana Haigis, Rebecca C Blum, Allyson Sherman-Roe, Mandy Pereira, Havovi Chichger, Corey E. Ventetuolo, Olin D Liang, Elizabeth O. Harrington#1610374) were used as a molecular weight ladder. Proteins were transferred onto PVDF membranes using the Trans-Blot® Turbo™ Mini PVDF Transfer Pack (Bio-Rad #1704156) on a Trans-Blot Turbo system at 2.5 A (constant) up to 25

(16) Selective Packaging of miR-22 and miR-29 Orthologues into Extracellular Vesicles of the Porcine Whipworm Trichuris SuisJ Extracell VesiclesAugust 29, 2026Bradley Whitehead, Maibritt Mardahl, Eline P. Hansen, Bastian Fromm, Anne Borup, Anders Toftegaard Boysen, Jasmine C. L. Atay, Rikke Nørregaard, Allan Stensballe, Sidsel D. Andersen, et al.Hercules, CA, USA). Proteins from the gel were transferred onto a nitrocellulose membrane using the Trans‐Blot Turbo Transfer System (Bio‐Rad, Hercules, CA, USA). Total protein bands were visualised using No‐Stain Protein Labelling Reagent

(17) Diurnal Regulation of Hippocampal Synaptic Plasticity in Aged Rats and the Role of the Circadian Clock Gene Per1HippocampusAugust 28, 2026Liam T. Ralph, Shinwon Kang, Lauren A. Joe, Patrick Tidball, Ahmad R. Israwi, Meng Tian, Ashish Kadia, Lijia Zhang, Fuzi Jin, Rachael Ingram, et al.transferred to 0.45 μm low‐fluorescence PVDF membranes (Bio‐Rad, 1620264) at 25 V for 3 min using the Trans‐Blot Turbo system (Bio‐Rad, 1704150EDU) in Trans‐Blot Turbo buffer (Bio‐Rad, 10026938) prepared as described by

(18) Prefoldin function links meiotic chromosome segregation with cellular remodeling and reveals tubulin sensitivity of the meiotic spindleSci AdvAugust 28, 2026Naohiro Kuwayama, Benjamin S. Styler, Bojana Stekovic, Helen Sakharova, Liana Lareau, Marko Jovanovic, Elçin Ünal, Gloria A. Braracrylamide gel, separated at 150 V for 50 min, and transferred to a nitrocellulose membrane using the TransBlot Turbo system (Bio-Rad). Blots were blocked and probed overnight at 4°C with one or more of the following antibodies:

(19) Elevation of body temperature during exercise enhances protection against dexamethasone‑induced skeletal muscle atrophyExp PhysiolAugust 28, 2026Berkay Ozerklig, Senay Akin, Seda Olgaz Bingol, Bulent Okan Yildiz, Scott K. Powers, Ibrahim Turkel, Haydar A. DemirelFollowing electrophoresis, proteins were transferred onto nitrocellulose membranes using a semi‐dry Trans‐Blot Turbo system (Bio‐Rad) for 30 min at 25 V. Membranes were blocked for 1 h at room temperature in Tris‐buffered

(20) Protein-Protein Interactions Between Peptidoglycan, Lipopolysaccharide, and Phospholipid Biosynthesis Enzymes in Escherichia coliChembiochemAugust 27, 2026Lea-Janina Tilg, Sophia Weber, Hannah Bille, Anna-Maria Möller, Franz Narberhaus25% (v/v) methanol and 10% (v/v) acetic acid overnight. Western Blot transfer was carried out via the Trans‐Blot Turbo Transfer System (Bio‐Rad). For the in vivo degradation, a Ponceau S staining (0.01% (w/v) Ponceau S, 1% (v/v) acetic

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(1)

3/28/2025Fast and Reliable Protein Transfer System From Bio Rad for Western Bots Pavan Kumar ChodisettiWe utilized the Bio-Rad Trans-Blot® Turbo™ Transfer System to transfer proteins from SDS-PAGE gels to PVDF membranes for the immunodetection of cell wall hydrolases in Enterococcus faecium. Our ... Read Review
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5/24/2023Old but Reliable Blot Transfer Equipment Manuel Jr VidalWe do Western blots on a regular basis. This equipment has always been the go-to equipment for our blot transfers.
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7/6/2022Fast and Efficient Trans-Blot Turbo™ Transfer System Niroj ShresthaThe proteins from HEK293 cell lysate were resolved in NuPAGE 4%–12% Bis-Tris gel and transferred to 0.2-µm PVDF membrane for 7 minutes with the Trans-Blot Turbo Transfer System and kit as per ... Read Review

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