Fig 1: Foxp3 downregulation by Tregs associates with ADAMTSL5 autoantibodies in psoriatic disease. Association of ADAMTSL5 autoantibodies as measured by ELISA with Foxp3 expression by Tregs as measured by flow cytometry. (A) ELISA of anti-ADAMTSL5 IgG in sera of HC (n = 35), psoriasis patients (n = 39), PsA patients (n = 39), and synovial fluid of PsA patients (n = 43). *P value < 0.05. (B–E) Scatterplots of significant Spearman’s rank correlation of serum anti-ADAMTSL5 autoantibody concentration with Foxp3 expression by CD3 + CD4 + CD25 + CD127lo T cells derived from peripheral blood of psoriasis (n = 21) and PsA patients (n = 20). Differentiation between Foxp3int (B, C) and Foxp3hi (D, E) Tregs in patients with psoriatic disease (B, D) versus PsA patients only (C, E). ADAMTSL5: A Disintegrin And Metalloprotease domain containing ThromboSpondin type 1 motif-Like 5; Foxp3int/-hi: forkhead box P3 expression intermediate / high; HC: healthy control; IgG: Immunoglobulin G; P: p value; PsA: psoriatic arthritis; PsA: psoriatic arthritis; PsO: psoriasis; ρ: Spearman’s rho.