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GoTaq G2 DNA Polymerase
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GoTaq G2 DNA Polymerase
GoTaq G2 DNA Polymerase
GoTaq G2 DNA Polymerase from Promega
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Citations:
(72)
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GoTaq G2 DNA Polymerase
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Description
GoTaq G2 DNA Polymerase is supplied with 5X Green GoTaq Reaction Buffer and 5X Colorless GoTaq Reaction Buffer
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
GoTaq G2 DNA Polymerase
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
M7848
Size
2,500u
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Promega
2800 Woods Hollow Rd
Madison
,
WI
53711
United States
Phone
: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516
Email: custserv@promega.com
techserv@promega.com
Company Profile
Website:
http://www.promega.com
Citations (72)
(1)
Exome sequencing enables molecular diagnosis in 10% of early-onset or familial systemic lupus erythematosus cases
EBioMedicine
April 1, 2026
Maud Tusseau, Samira Khaldi-Plassart, Audrey Labalme, Anne-Laure Mathieu, Quentin Riller, Corentin Molitor, Thomas Simonet, Sebastien Viel, Christine Gaboriaud, Nicole Thielens,
et al.
manufacturer's protocol. cDNA
amplification
was subsequently carried out using
GoTaq G2 DNA Polymerase
(
Promega
,
M7841
).
IKZF3
expression The plasmid pTwist CMV, with either wild-type or mutated
IKZF3
(NM_001257414) cDNA
(2)
The microcephaly-associated protein YIPF5 differentially regulates ER export
iScience
February 20, 2026
Francesca Bruno, Mihaela Anitei, Domenico Di Fraia, William Durso, Therese Dau, Emilio Cirri, Mara Sannai, Christina Valkova, Julija Maldutyte, Elizabeth A. Miller,
et al.
inhibitor
cocktail,Roche,Cat#5056489001 CHAPSO,Carl ROTH,Cat#82473-24-3 GoTaqG2 polymerase,
Promega
,Cat#
M7841
NEBuilder® HiFi DNA Assembly Master Mix
,
New England Biolabs
,Cat#
E2621
Dulbecco’s Modified Eagle Medium
(3)
De novo cysteine biosynthesis in Pseudomonas aeruginosa: Characterization of the two main cysteine synthase isoforms
iScience
January 16, 2026
Rebecca Martedì, Jole Maria Lucia D’Angelo, Giulia Sassi, Marialaura Marchetti, Sarah Hijazi, Riccardo Percudani, Stefano Bettati, Barbara Campanini, Emanuela Frangipani
"
T4 DNA Ligase
, 100u",
Promega
Italia Srl,Cat#
M1801
"GoTaq(R) G2 DNA Polymerase, 100u",
Promega
Italia Srl,Cat#
M7841
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, "Wizard(R)
(4)
Rethinking genomics of facioscapulohumeral muscular dystrophy in the telomere-to-telomere era: pitfalls in the hidden landscape of D4Z4 repeats
European Journal of Human Genetics
January 14, 2026
Valentina Salsi, Francesca Losi, Sara Pini, Matteo Chiara, Rossella Tupler
semi-
quantitative PCR
was performed with 50 ng of genomic DNA using
GoTaq® G2 DNA Polymerase
(
Promega
, cat. #
M7841
) under standard cycling conditions, with annealing temperatures optimized around 60°C. For targets producing
(5)
Unbiased and comprehensive identification of virus-derived circular RNAs in a large range of viral species and families
PLOS Pathogens
September 11, 2025
Alexis S. Chasseur, Maxime Bellefroid, Mathilde Galais, Meijiao Gong, Pierre Lombard, Sarah Mathieu, Amandine Pecquet, Estelle Plant, Camille Ponsard, Laure Vreux,
et al.
control was produced. The PCRs were carried out using primers detailed in S1 Table and the GoTaq G2 kit (
M7841
–
Promega
) or the Q5
taq polymerase
(
M0491L
–
New England Biolabs
). The cycles were adapted to the manufacturers’
(6)
A simple and rapid shaking-based assay to genotype live, early developmental stage zebrafish embryos
Laboratory Animals
August 14, 2025
Rima Siauciuinate, Christelle Etard, Almut Koehler, Odelia Pisanty, Miriam Otto, Thomas Dickmeis, Olivier Kassel, Yoav Gothilf, Nicholas S Foulkes, Daniela Vallone
Roth ref. 9127.2);0.025 mg/ml PK;
PCR reactions
:GoTaq Polymerase and GoTaq Green buffer (
Promega
, ref.
M7845
);dNTPs 10 mM Mix (
Promega
, ref.
U1511
);
PCR
primers (Sigma-Aldrich);DNA-ladder 100 bp (
New England BioLabs
,
(7)
Establishing a pediatric solid tumor PDX biobank for precision oncology research
Cancer Biol Ther
August 13, 2025
Larissa Akemi Kido, Milena Rodrigues Marusco, Ellen Aparecida da Silva, Laís Do Carmo, Ana Beatriz Teodoro Borges, Felipe Luz Torres Silva, Juliana Silveira Ruas, Dieila Giomo de Lima, Larissa de Abreu Fernandes, Camila Maia Martin Daiggi,
et al.
translocation analysis.
PCR reactions
were performed using GoTaq G2 polymerase (
Promega
™, Madison, WI, USA, Cat#
M7841
), specific primers for target translocations and optimized thermal cycling conditions. The translocations
(8)
A chimeric Mla-Pqi lipid transport system is required for Brucella abortus survival in macrophages
The EMBO Journal
August 13, 2025
Adélie Lannoy, Alexi Ronneau, Miguel Fernández-García, Marc Dieu, Patricia Renard, Antonia García Fernández, Raquel Condez-Alvarez, Xavier De Bolle
acid,
ThermoFisher
,B25096 gentamicin,
Sigma
,
G1397
ampicillin,Sigma,A9518
Q5® High-Fidelity DNA Polymerase
,
New England Biolabs
,A9518
GoTaq® DNA polymerase
,
Promega
,
M7848
T4 DNA ligase
,
Invitrogen
,100004917
EcoRV
,
New England Biolabs
,
R3195S
Sodium deoxycholate,VWR,0613-50
(9)
Mitochondrial dynamics and quality control regulate proteostasis in neuronal ischemia-reperfusion
Autophagy
July 1, 2025
Fogo GM, Raghunayakula S, Emaus KJ, Torres Torres FJ, Shangguan G, Wider JM, Hüttemann M, Sanderson TH.
Reaction Buffer (Promega, M791A),
dNTP mix
(
Promega
,
U1515
), and
GoTaq G2 DNA polymerase
(
Promega
,
M7848
).
PCR
products were run on 1.5% agarose gels with 1X GelRed Nucleic Acid Stain (MilliporeSigma, SCT123)
(10)
Physiological role and mechanisms of action for a long noncoding haplotype region
Cell Reports
June 24, 2025
Hong Xue, Manoj K. Mishra, Yong Liu, Pengyuan Liu, Michael Grzybowski, Rajan Pandey, Kristie Usa, Mark A. Vanden Avond, Niharika Bala, Abdel A. Alli,
et al.
Triton X-100 Surfact-Amps Detergent Solution
,
Thermo Scientific
,Cat#
85111
Tween 20
,
Bio-Rad
,Cat#
1706531
GoTaq G2 DNA Polymerase
,
Promega
,Cat#
M7841
Q5 Hi-fidelity DNA polymerase,
NEB
,Cat#
M0491S
2.5% normal
horse
serum,
Vector Labs
,Cat#
S-2012-50
Vectashield
(11)
Senescence and inflammation are unintended adverse consequences of CRISPR-Cas9/AAV6-mediated gene editing in hematopoietic stem cells
Cell Rep Med
June 17, 2025
Anastasia Conti, Kety Giannetti, Federico Midena, Stefano Beretta, Nicolò Gualandi, Rosaria De Marco, Edoardo Carsana, Angelica Varesi, Teresa Tavella, Laura Alessandrini,
et al.
,
New England Biolabs
,Cat#
M0302L
TaqMan PreAmp Master Mix
2X,
Thermo Fisher
,Cat#
4488593
GoTaq G2 DNA Polymerase
,
Promega
,Cat#
M7841
MinElute PCR Purification kit,
QIAGEN
,Cat#
28004
QuickExtract DNA Extraction Solution,Lucigen,Cat#QE09050 DNeasy
(12)
Functional Characterization of LTR12C as Regulators of Germ-Cell-Associated TA-p63 in U87-MG and T98-G In Vitro Models
Cells
June 5, 2025
Lucia Meola, Sohum Rajesh Shetty, Angelo Peschiaroli, Claudio Sette, Camilla Bernardini
20 μL was amplified by conventional
polymerase chain reaction (PCR
) using GoTaq polymerase (
Promega
,
M7841
, Madison, WI, USA) and analyzed by
agarose gel electrophoresis
. Amplified DNA was isolated from the
(13)
Perinatal death in pig models of hypertrophic cardiomyopathy carrying sarcomere pathogenic variants
Journal of Molecular and Cellular Cardiology Plus
June 1, 2025
Tatiana Flisikowska, Björn Petersen, Giulia Mearini, Daniela Huber, Mayuko Kurome, Melanie Stoff, Saskia Schlossarek, Andrea Lucas-Hahn, Eckhard Wolf, Judith Montag,
et al.
5’-GATGCAGAGCTGCTCAAAGCTGTT-3′ resulting in an amplicon of 492 bp. PCRs were carried out using GoTaq polymerase (
Promega
,
M7841
) with the following thermal cycling parameters: 2 min, 95 °C then 45 cycles of: 30 s, 95 °C; 30 s, 60
(14)
Mitochondria regulate MR1 protein expression and produce self-metabolites that activate MR1-restricted T cells
Proc Natl Acad Sci U S A
May 20, 2025
Gennaro Prota, Giuliano Berloffa, Wael Awad, Alessandro Vacchini, Andrew Chancellor, Verena Schaefer, Daniel Constantin, Dene R. Littler, Rodrigo Colombo, Vladimir Nosi,
et al.
ND5-, and GAPDH-specific primer pairs were used in PCRs with
GoTaq G2 DNA polymerase
(
Promega
, Cat#
M7845
).
Clones
were generated by limiting dilution and expansion.
PCR
confirmed the lack of mitochondrial
(15)
Capsular Polysaccharide Restrains Type VI Secretion in Acinetobacter baumannii
Elife
January 3, 2025
Nicolas Flaugnatti, Loriane Bader, Mary Croisier-Coeytaux, Melanie Blokesch
,
New England Biolabs
,
M0491L
,Polymerase for
PCR
Commercial kit or
assay
,
GoTaq G2 DNA polymerase
,
Promega
,
M7848
,Polymerase for
PCR
Recombinant DNA reagent,Plasmids,This study,Plasmids,See Supplementary file 1 for
(16)
Loop-extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex
Molecular Cell
January 2, 2025
Biswajit Pradhan, Amar Deep, Jessica König, Martin Dieter Baaske, Kevin D. Corbett, Eugene Kim
handles were made using a
PCR
on pBluescript SK+ (Stratagene) with
GoTaq G2 DNA polymerase
(
Promega
,
M7845
). The
PCR
was done using biotinylated primers primer CD21 (GACCGAGATAGGGTTGAGTG) and CD22 (CAGGGTCGGAACAGGAGAGC),
(17)
Methods for investigating STAT3 regulation of lysosomal function in mammary epithelial cells
Journal of Mammary Gland Biology and Neoplasia
December 1, 2024
Bethan Lloyd-Lewis, Michael E. D’Angelo, Neve B. Prowting, Bethan E. Wiseman, Timothy J. Sargeant, Christine J. Watson
equivalent).Cryovials.Genomic DNA extraction kit (e.g.
69504
,
QIAGEN
).Thermostable DNA polymerase (e.g.
M7841
,
Promega
).PCR clean-up kit (e.g.
28106
,
QIAGEN
).Primary and secondary antibodies to assess
STAT3
knockdown
(18)
Heterochromatin-dependent transcription links the PRC2 complex to small RNA-mediated DNA elimination
EMBO Reports
November 29, 2024
Therese Solberg, Chundi Wang, Ryuma Matsubara, Zhiwei Wen, Mariusz Nowacki
other reagents"
Q5 high-fidelity DNA polymerase
,
NEB
,Cat#
M0491L
GoTaq G2 DNA polymerase
,
Promega
,Cat#
M7848
RadPrime DNA Labeling System
,
Invitrogen
,Cat#
18428011
Wheat
grass
powder,"Pines International, Lawrence,
(19)
Outbreak of Western Equine Encephalitis Virus Infection Associated with Neurological Disease in Horses Following a Nearly 40-Year Intermission Period in Argentina
Viruses
October 10, 2024
María Aldana Vissani, Florencia Alamos, María Silvia Tordoya, Leonardo Minatel, Juan Manuel Schammas, María José Dus Santos, Karina Trono, María E. Barrandeguy, Udeni B. R. Balasuriya, Mariano Carossino
PCR
. The 25 μL reaction contained 5 μL of
PCR
Green Buffer with magnesium chloride (
Promega
, Cat N°
M7845
, Madison, WI, USA), 1 μL of 5 mM dNTP, 0.75 μL of 50 mM magnesium chloride, 0.25 μL of
GoTaq DNA polymerase
(20)
Ablation of PC1/3 in POMC-Expressing Tissues but Not in Immune Cells Induces Sepsis Hypersensitivity
Journal of the Endocrine Society
September 26, 2024
Moeller J, Meier DT.
1.25 μL was used for the
polymerase chain reaction (PCR
) reaction employing
GoTaq G2 DNA Polymerase
(
M7845
,
Promega
, Dübendorf, Switzerland) with primers (Microsynth, Balgach, Switzerland) on a Biometra TRIO
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GoTaq G2 DNA Polymerase from Promega
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