PNGase F V4831 from Promega

Product Specs
ItemPNGase F
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberV4831
Size500 units

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Enzymatically Controlled Release of Proteins and Peptides (ENCOREP): A Promising Alternative Secretion ApproachiScienceApril 17, 2026Daniel Ivanusic, Gregor Zipf, Josef Maier, Hubert Bernauer, Norbert BannertTEV Protease,New England Biolabs,Cat# P8112S PNGase F,New England Biolabs,Cat# P0704S PNGase F,Promega,Cat# V4831 Benzonase Nuclease,Millipore,Cat# 70746-10KU "Sambucus nigra lectin (SNA, EBL) biotinylated",Vector Laboratories

(2) Deficient de-S-acylation in aging and CLN1 contributes to lyso-mitochondrial dysfunction, lipid dyshomeostasis, and resultant lipofuscin biogenesisResearch SquareFebruary 10, 2026Sofia Massaro Tieze, Alexander Esqueda, Rachel McAllister, Matija Lagator, Betül Yücel, Eric Sun, TuKiet T. Lam, Nicholas Lockyer, Kallol Gupta, Sreeganga S. Chandrathe dark). Reduced and alkylated proteins were then treated with PNGase (37°C, overnight, Promega, V4831) followed by digestion with Lys-C (1:50 enzyme:protein ratio; 37°C, 6.5 hours) and subsequently with

(3) Breast cancer bone metastasis and bone metastatic cells retain NKG2DLs intracellularly: could this be a strategy to evade immune recognition?Frontiers in Cell and Developmental BiologyJanuary 27, 2026Marta Gomarasca, Paola Maroni, Chiara Verdelli, Laura Gerosa, Martina Faraldi, Alessandro Luzzati, Luca Cannavò, Giuseppe Banfi, Giovanni Lombardicell lysates were digested with either 1500 U of EndoH (V4871, Promega) for 4 h or 20 U of PNGase F (V4831, Promega) for 2 h, according to the manufacturer’s instructions. Following enzymatic digestion, MICA/B

(4) Improving dual targeting selectivity in T-cell engagers via synapse-gated and affinity-tuned trispecific antibody designMAbsOctober 8, 2025Peng Zhao, John Schardt, Chi-I Chiang, Pooja Shah, Gee Sung Eun, Jan Martinek, Matthew Cyr, Yoshimi Johnson, Bismark Amofah, Xiaoying Ye, et al.established protocol with minor modifications.19 Proteins were deglycosylated using PNGase F (Promega V4831) before intact mass analysis or digested using IgdE (Genovis, A0-AG1-020) and/or GlySERIAS (Genovis,

(5) Molecular mechanisms of immune evasion by host protein glycosylation of a bacterial immunogen used in nucleic acid vaccinesbioRxivMay 31, 2025Mukaddes Sena Cinar, Trevor M Adams, Ahmet Ozdilek, Fikri AvciLaboratories, Part #: DLM-4–100). Glycopeptides were treated with 2μL/20 units PNGaseF (Promega, Part #: V4831) overnight at 37°C. Deglycosylated peptides were then subjected to a final round of reverse-phase cleanup

(6) Glycomics and Glycoproteomics Reveal Distinct Oligomannose Carriers Across Bladder Cancer StagesInt J Mol SciMay 20, 2025Marta Relvas-Santos, Dylan Ferreira, Andreia Brandão, Luis Pedro Afonso, Lúcio Lara Santos, André M. N. Silva, José Alexandre Ferreiratissue slide with PNGase F from Elizabethkingia miricola (1 U/10 µg protein at 37 °C overnight; Promega, V4831, Madison, WI, USA). The glycosylamine form of released N-glycans was hydrolyzed on glass tubes by incubation

(7) In-depth immunochemical characterization of the serum antibody response using a dual-titration microspot assayFrontiers in ImmunologyFebruary 25, 2025Ágnes Kovács, Zoltán Hérincs, Krisztián Papp, Jakub Zbigniew Kaczmarek, Daniel Nyberg Larsen, Pernille Stage, László Bereczki, Eszter Ujhelyi, Tamás Pfeil, József Prechltreated with 2% homemade methylated trypsin [1] for an hour at 57 degrees and PNGaseF (0.5 µl) (Promega, V4831) for an hour at 37 degrees, while the other was only treated with 2% trypsin for an hour at 57 degrees. The

(8) Veneer Is a Webtool for Rapid, Standardized, and Transparent Interpretation, Annotation, and Reporting of Mammalian Cell Surface N-Glycocapture DataJournal of Proteome ResearchAugust 2, 2024Linda Berg Luecke, Roneldine Mesidor, Jack Littrell, Morgan Carpenter, Melinda Wojtkiewicz, Rebekah L. Gundryof PNGase F from Four Different Manufacturers PNGase F from New England Biolabs (P0709S), Promega (V4831), and Roche (11365169001) was used as provided, and PNGase F PRIME-LY (N-Zyme Scientifics NZPP010LY)

(9) N-Glycosylation Profiles of Immunoglobulin G and Future Cardiovascular EventsCirculation ResearchMarch 1, 2024Rosangela A. Hoshi, Branimir Plavša, Yanyan Liu, Irena Trbojević-Akmačić, Robert J. Glynn, Paul M. Ridker, Richard D. Cummings, Ivan Gudelj, Gordan Lauc, Olga V. Demler, et al.BIA Separations),25 while N-glycans were released by peptide:N-glycosidase F (PNGase F, catalog no. V4831, Promega) and labelled with a fluorescent dye, 2-aminobenzamide (catalog no. A89804, Sigma-Aldrich),

(10) Identification of tyrosine sulfation in the variable region of a bispecific antibody and its effect on stability and biological activitymAbsDecember 31, 2023Christopher B. Lietz, Ekaterina Deyanova, Younhee Cho, Jon Cordia, Sarah Franc, Sally Kabro, Steven Wang, David Mikolon, Douglas D. Banksby incubating 1 mg/mL of mAb-X buffered by 50 mM Tris-HCl (pH 7.5) with PNGase F (Promega, Catalog # V4831) at a final enzyme concentration of 1 U/µL. Samples were incubated overnight at 37°C prior to intact

(11) CAR T cells as micropharmacies against solid cancers: Combining effector T-cell mediated cell death with vascular targeting in a one-step engineering processCancer Gene TherapyOctober 1, 2023Bianca Altvater, Sareetha Kailayangiri, Christian Spurny, Maike Flügge, Jutta Meltzer, Lea Greune, Katja Urban, Christian Schwöppe, Caroline Brand, Christoph Schliemann, et al.ice 2 µl GlycoBuffer 2 (10X), 2 µl 10% NP-40, 1 µl PNGase F (Promega, Walldorf, Germany, product no. V4831) and 6 µl H2O were added, mixed and incubated at 37 °C for 1 h. Factor X (FX) activation assay The

(12) Analyzing the topology of N-linked glycans by PNGase F accessibility assaySTAR protocolsSeptember 15, 2023Wang J, Ye J.proteins" ,, Pierce Protease Inhibitor (Cocktail) Mini Tablets,Thermo Fisher,A32955 PNGase F,Promega,V4831 "0.5 M Tris-HCl, pH 6.8",Bio-Rad,1610799 (PBS) Dulbecco’s Phosphate-Buffered Saline without calcium

(13) Development of an Affinity-Based Probe to Profile Endogenous Human Adenosine A3 Receptor ExpressionJournal of Medicinal ChemistryAugust 24, 2023Bert L. H. Beerkens, Inge M. Snijders, Joep Snoeck, Rongfang Liu, Anton T. J. Tool, Sylvia E. Le Dévédec, Willem Jespers, Taco W. Kuijpers, Gerard J.P. van Westen, Laura H. Heitman, et al.pH 7.4). Eosinophils were isolated from the PMNs as described before.68 Biologicals PNGase (cat# V4831) was purchased from Promega (Leiden, The Netherlands). RabbitαhA3AR antibody (cat# bs-1225R) was ordered

(14) Multi-Attribute Monitoring Method for Process Development of Engineered Antibody for Site-Specific ConjugationJournal of the American Society for Mass SpectrometryJuly 5, 2023Alistair R. Hines, Matthew Edgeworth, Paul W. A. Devine, Samuel Shepherd, Nicholas Chatterton, Claire Turner, Kathryn S. Lilley, Xiaoyu Chen, Nicholas J. Bondconducted through UPLC analysis. Antibody intermediate (100 mg) was digested overnight with PNGaseF (V4831, Promega) at 37 °C, and subsequently labeled with 2-aminobenzamide (PN 654213, Sigma-Aldrich) for 30

(15) Comprehensive glycoprofiling of oral tumours associates N-glycosylation with lymph node metastasis and patient survivalMolecular & Cellular ProteomicsJuly 1, 2023Carolina Moretto Carnielli, Thayná Melo de Lima Morais, Fábio Malta de Sá Patroni, Ana Carolina Prado Ribeiro, Thaís Bianca Brandão, Evandro Sobroza, Leandro Luongo Matos, Luiz Paulo Kowalski, Adriana Franco Paes Leme, Rebeca Kawahara, et al.N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μl) for 16 h at 37 °C. The detached N-glycans were reduced with 1 M sodium borohydride in 50 mM

(16) Virus-Receptor Interactions of Glycosylated SARS-CoV-2 Spike and Human ACE2 ReceptorbioRxivApril 26, 2023Peng Zhao, Jeremy L. Praissman, Oliver C. Grant, Yongfei Cai, Tianshu Xiao, Katelyn E. Rosenbalm, Kazuhiro Aoki, Benjamin P. Kellman, Robert Bridger, Dan H. Barouch, et al.,Sigma Aldrich,Cat#I1149 Trypsin,Promega,Cat#V5111 Lys-C,Promega,Cat#V1671 Arg-C,Promega,Cat#V1881 Glu-C,Promega,Cat#V1651 Asp-N,Promega,Cat#VA1160 Endoglycosidase H,Promega,Cat#V4871 PNGaseF,Promega,Cat#V4831 Chymotrypsin,Athens Research and Technology,Cat#16-19-030820 Alpha lytic protease,New England BioLabs,Cat#P8113 18O

(17) Topological regulation of a transmembrane protein by luminal-to-cytosolic retrotranslocation of glycosylated sequenceCell reportsApril 25, 2023Wang J, Han S, Ye J.were incubated in 50 mM sodium phosphate in the absence or presence of 0.25 U/μl PNGase F (Promega V4831), 0.5% NP-40, and/or 0.5% SDS at 30°C for 45 min. After cooling to 4°C, the reaction was terminated

(18) Preclinical safety and efficacy of a therapeutic antibody that targets SARS-CoV-2 at the sotrovimab face but is escaped by OmicroniScienceApril 21, 2023Kreye J, Reincke SM, Edelburg S, Jeworowski LM, Kornau HC, Trimpert J, Hombach P, Halbe S, Nölle V, Meyer M, et al.MabSelectTM,Cytiva,Cat# 28408256 MACSQuant® Tyto® Cartridges,Miltenyi Biotec,Cat# 130-104-791 PNGase F,Promega,Cat# V4831 OCT medium (Roth).,Roth,Cat# 6478.1 Ventana diluent,Ventana,Cat# 760-108 DAB Map detection system,Ventana,Cat#

(19) Cyclophosphamide depletes tumor infiltrating T regulatory cells and combined with anti-PD-1 therapy improves survival in murine neuroblastomaiScienceSeptember 16, 2022Webb ER, Moreno-Vincente J, Easton A, Lanati S, Taylor M, James S, Williams EL, English V, Penfold C, Beers SA, et al.Biosciences,https://www.bdbiosciences.com/en-gb/products/software/instrument-software/bd-facsdiva-software ,, Other,Other,Other ,, PNGase F,Promega,Cat#V4831 Liberase TL,Merck (Roche),Cat#V4831 DNAse,Sigma-Aldrich,Cat#10104159001 100 μm cell strainer,Corning,Cat#352360 Red

(20) An optimized protocol for PD-L1 pathological assessment with patient sample deglycosylation to improve correlation with therapeutic responseSTAR ProtocolsMarch 18, 2022Ying-Nai Wang, Heng-Huan Lee, Yongkun Wei, Yu-Yi Chu, Weiya Xia, Michael Wang, Dihua Yu, Mien-Chie Hung(Cat#P0704). However, it should be also applicable to that from different vendors, such as from Promega (Cat#V4831) or Sigma-Aldrich (Cat#G5166). We have not evaluated it using other PNGase F proteins, but we extrapolate

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PNGase F

PNGase F from Promega

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PNGase F from Promega

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