Human IL-2 IS, premium grade 130-097-744 from Miltenyi Biotec

(1) Healthy donor T cell receptors expand functional neoantigen recognition beyond patient vaccinationSci AdvApril 17, 2026Hong Kai Teo, Shuting Han, Thamizhanban Manoharan, Yi Ren, Cyrus Zai Ming Cheng, Soon Chai Loo, Zhewang Lin, Jiaqi Li, Who-Whong Wang, Si-Lin Koo, et al.the CD8+ T cells were resuspended with RPMI 1640 with 10% HS, 1% P/S, and IL-2 (600 IU/ml; Miltenyi, #130-097-744) and mixed with 50 μl of lentivirus in the presence of polybrene (10 μg/ml) and centrifuged at 2000g

(2) Identification of immunogenic neoantigens from intron retention in colorectal cancerScientific ReportsMarch 9, 2026Thamizhanban Manoharan, Brandon Bing Rui Kee, Cyrus Zai Ming Cheng, Malcolm Kaiheng Choy, Bei En Siew, Wai-Kit Cheong, Kai-yin Lee, Ian Jse-Wei Tan, Bettina Lieske, Choon Kong Yap, et al.coculture, on day four, the expansion media (RPMI 1640 with 10% HS, 2% P/S, 200 IU/ml IL-2 (Miltenyi #130-097-744), plus 40 ng/ml IL-7 and IL-15) was added to the co-cultured cells. Expansion medium was replenished

(3) CD8+ T cells in the tumor microenvironment modulate the response to endocrine therapy in breast cancerJournal of Clinical InvestigationFebruary 2, 2026Fabiana Napolitano, Yunguan Wang, Dhivya R. Sudhan, Paula I. Gonzalez-Ericsson, Luigi Formisano, Nisha Unni, Shahbano Shakeel, James Z. Zhu, Khushi Ahuja, Lei Guo, et al.antibiotic/antimycotic (Gibco, Thermo Fisher Scientific) plus IL-2 improved sequence (Miltenyi Biotec, 130097744), at 37°C in a humidified atmosphere of 5% CO2. Cell lines were authenticated by ATCC by the short tandem

(4) Alternative polyadenylation upon CPSF6 knock-out enhances HIV-1 infection in primary T cellsPLoS PathogDecember 12, 2025Daphne Cornish, Kathryn A. Jackson-Jones, Ted Ling-Hu, Lacy M. Simons, William J. Cisneros, Edmund Osei Kuffour, Natalie Stegman, Francesca Agnes, Yujin Lee, Ved P. Sharma, et al.#SV30010). Media was supplemented with interleukin-2 (IL-2) at 20 IU/mL immediately before use (Miltenyi, #130097744). Cells were activated on α-CD3-coated plates (Tonbo Biosciences, UCHT1) incubated at 4°C overnight

(5) Protocol for the simultaneous activation and lentiviral transduction of primary human T cells with artificial T cell receptorsSTAR ProtocMarch 21, 2025Jordan Yong Ming Tan, Jia Chi Tan, Chuqi Wang, Ling Wu, Nicholas R.J. Gascoigne, Shruti Bhattproteins" ,, "Recombinant human interleukin-2 (IL-2) improved sequence (IS), premium grade",Miltenyi Biotec,Cat# 130-097-744 "Miltenyi T Cell TransAct, human",Miltenyi Biotec,Cat# 130-111-160 "Gibco Trypsin-EDTA (0.5%), no

(6) Dose-dependent serological profiling of AdCLD-CoV19-1 vaccine in adultsmSphereJanuary 28, 2025Jung Hyuk Lee, Yuna Shin, Kwang-Soo Shin, Ju Yeon Park, Mi Sun Kim, Young-Shin Park, Wuhyun Kim, Joon Young Song, Ji Yun Noh, Hee Jin Cheong, et al.media (#5150, StemCell) supplemented with 10% horse serum (#16050-122, StemCell), 0.02 µg/mL IL-2 IS (#130-097-744, Miltenyi Biotec), and 1% penicillin-streptomycin (#15140122, Invitrogen). For NK cell detection, anti-CD107a-PE-Cy5

(7) MCP5, a methyl-accepting chemotaxis protein regulated by both the Hk1-Rrp1 and Rrp2-RpoN-RpoS pathways, is required for the immune evasion of Borrelia burgdorferiPLOS PathogensDecember 30, 2024Sajith Raghunandanan, Kai Zhang, Yan Zhang, Raj Priya, Ching Wooen Sze, Yongliang Lou, Michael J. Lynch, Brian R. Crane, Mark H. Kaplan, Chunhao Li, et al.maintained in MyeloCult H5100 media (StemCell Technologies, 05150) containing IL-2 (20 ng/ml, Miltenyi, 130-097-744) and Horse Serum (12.6%, Gibco, 16050122). The human THP-1 cell line was used from our lab cell lines

(8) Optogenetically engineered Septin-7 enhances immune cell infiltration of tumor spheroidsProceedings of the National Academy of Sciences of the United States of AmericaOctober 29, 2024Chen J, Hnath B, Sha CM, Beidler L, Schell TD, Dokholyan NV.grew mouse primary CD8+ T-cells in the above RPMI media supplemented with 30 U/mL IL-2 (Miltenyi, Cat# 130-097-744) and Dynabeads™ mouse T-activator CD3/CD28 (Thermo Fisher Scientific, Cat# 11456D). We cultured NK92

(9) Migration of human T cells can be differentially directed by electric fields depending on the extracellular microenvironmentiScienceMay 17, 2024Karen Ende, Fabião Santos, Judith Guasch, Ralf Kemkemeradhesion molecule 1) (200 μg/mL),Invitrogen,Cat# A42523 Human IL-2 (interleukin-2),Miltenyi Biotec,Cat# 130-097-744 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, MACS negative

(10) Patient-derived tumoroids and proteomic signatures: tools for early drug discoveryFrontiers in ImmunologyApril 18, 2024Hélène Lê, Jules Deforges, Pasquale Cutolo, Anissa Lamarque, Guoqiang Hua, Véronique Lindner, Shreyansh Jain, Jean-Marc Balloul, Nadia Benkirane-Jessel, Eric Quéméneur50X,17504-044,1X VEGF-165,H9166-10µg,50ng/mL HGF,SRP6014-10µg,30ng/mL FGF-2,130-093-839-10µg,20ng/mL EGF,GF316-500µg,100ng/mL IL-2,"130-097-744, Miltenyi Biotech",20UI/mL PDTs and PDHOs culture media composition.

(11) Protocol for the detection of defined T cell clones in a heterogeneous cell populationSTAR protocolsMarch 15, 2024Gobbini A, Bandera A, Grifantini R, Abrignani S, Notarbartolo S.Milan, Italy. Notarbartolo et al.",N/A Recombinant human interleukin-2 (IL-2),Miltenyi Biotec,Cat. Num. 130-097-744 Phytohemagglutinin (PHA),Thermo Fisher Scientific Remel,Cat. Num. R30852801 BD Horizon brilliant stain

(12) Integrative analysis of neuroblastoma by single-cell RNA sequencing identifies the NECTIN2-TIGIT axis as a target for immunotherapyCancer cellFebruary 12, 2024Wienke J, Visser LL, Kholosy WM, Keller KM, Barisa M, Poon E, Munnings-Tomes S, Himsworth C, Calton E, Rodriguez A, et al.Doxorubicin hydrochloride,Sigma-Aldrich,Cat# D1515 Cyclophosphamide monohydrate,Sigma-Aldrich,Cat# C0768 IL-2,Miltenyi,Cat# 130-097-744 IL-15,Miltenyi,Cat# 130-095-762 ,, Critical commercial assays,Critical commercial assays,Critical

(13) Regulatory T cells in autoimmune primary adrenal insufficiencyClinical and Experimental ImmunologyJanuary 9, 2024Thea Sjøgren, Jan-Inge Bjune, Eystein S Husebye, Bergithe E Oftedal, Anette S B Wolff(Miltenyi Biotec, cat. 130-097-196) supplemented with 500 U/ml recombinant (r) IL2 (Miltenyi Biotec, cat. 130-097-744) and 5% human AB serum for 14 days at 37°C and 5% CO2, according to the Treg Expansion Kit (Miltenyi Biotec

(14) Multimodal cartography of human lymphopoiesis reveals B and T/NK/ILC lineages are subjected to differential regulationiScienceOctober 20, 2023Alhaj Hussen K, Chabaane E, Nelson E, Lekiashvili S, Diop S, Keita S, Evrard B, Lardenois A, Delord M, Verhoeyen E, et al."Human GM-CSF, premium grade",Miltenyi Biotech,Cat#130-093-866 "Human IL-2 IS, premium grade",Miltenyi Biotech,Cat#130-097-744 "Human IL-3, premium grade",Miltenyi Biotech,Cat#130-095-069 "Human IL-7, premium grade",Miltenyi Biotech

(15) Mapping the chemotactic landscape in NK cells reveals subset-specific synergistic migratory responses to dual chemokine receptor ligationeBioMedicineOctober 1, 2023Mieszko Lachota, Katarzyna Zielniok, Daniel Palacios, Minoru Kanaya, Leena Peena, Hanna Julie Hoel, Merete Thune Wiiger, Lise Kveberg, Wojciech Hautz, Radosław Zagożdżon, et al.stimulation, 1 or 10 ng/ml of IL-15 (Miltenyi, Cat# 130-095-765) or 100 IU/ml IL-2 (Miltenyi, Cat# 130-097-744) was added to R10 NK cell culture medium. NK cells were stimulated either for 24 h or 7 days. For NK cell

(16) GSK-3 Inhibitor Elraglusib Enhances Tumor-Infiltrating Immune Cell Activation in Tumor Biopsies and Synergizes with Anti-PD-L1 in a Murine Model of Colorectal CancerInternational journal of molecular sciencesJune 29, 2023Huntington KE, Louie AD, Srinivasan PR, Schorl C, Lu S, Silverberg D, Newhouse D, Wu Z, Zhou L, Borden BA, et al.containing 20% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin. Recombinant human IL-2 (Miltenyi cat# 130-097744) with a final concentration of 100 units/mL was added to all immune cell culture media. All cell lines

(17) GSK-3 inhibitor elraglusib enhances tumor-infiltrating immune cell activation in tumor biopsies and synergizes with anti-PD-L1 in a murine model of colorectal cancerbioRxiv : the preprint server for biologyFebruary 7, 2023Huntington KE, Louie AD, Srinivasan PR, Schorl C, Lu S, Silverberg D, Newhouse D, Wu Z, Zhou L, Borden BA, et al.containing 20% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin. Recombinant human IL-2 (Miltenyi cat# 130–097744) with a final concentration of 100 units/mL was added to all immune cell culture media. All cell lines

(18) Ribonuclease 1 Enhances Antitumor Immunity against Breast Cancer by Boosting T cell ActivationInternational journal of biological sciencesJanuary 1, 2023Wang YN, Lee HH, Jiang Z, Chan LC, Hortobagyi GN, Yu D, Hung MC.cultured in RPMI 1640 medium containing 10% fetal bovine serum and IL-2 (10 ng/ml; Miltenyi Biotec, #130-097-744) to maintain T cell activity. Treatment with recombinant RNase1 protein purified from HEK293 cells (Sino Biological Inc

(19) Evaluating immune response in vitro in a relevant microenvironment: a high-throughput microfluidic model for clinical screeningExploration of Targeted Anti-tumor TherapyDecember 29, 2022Flora Doffe, Layla Fuoco, Judith Michels, Sandra Jernström, Raphael Tomasi, Pierre SavagnerSaint-Quentin-Fallavier France, S2GPT01RE) supplemented with 50 units of interleukin-2 (IL-2, Miltenyi Biotec, 130-097-744). After 48 h we added the third drop for treatments. We used a CF™647 hydrazide barcode at 2.0 μmol/L

(20) Impaired TRPM3-dependent calcium influx and restoration using Naltrexone in natural killer cells of myalgic encephalomyelitis/chronic fatigue syndrome patientsJournal of Translational MedicineDecember 1, 2022Natalie Eaton-Fitch, Stanley Du Preez, Hélène Cabanas, Katsuhiko Muraki, Donald Staines, Sonya Marshall-Gradisnikreconstituted at 100 mM in distilled water. IL-2 was purchased from Miltenyi Biotec (product code: 130-097-744) stored at 100,000 IU stock in distilled water for up to 1 month. Ionomycin was purchased from Sigma

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1/10/2023NK Expansion with IL-2 Scarlette CASSALI use Recombinant human IL-2 Improved Sequence (IS) at 500 UI/mL to expand and activate NK cells. It works very well.

Human IL-2 IS, premium grade from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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Human IL-2 IS, premium grade from Miltenyi Biotec

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