Cultrex RGF Basement Membrane Extract, Type 2 3533-005-02 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex RGF Basement Membrane Extract, Type 2
CompanyR&D Systems, a Bio-Techne Brand
Price $292.00Supplier Page View Company Product Page
Catalog Number3533-005-02
Size5 mL
ApplicationsBioactivity
Format12 - 18 mg/mL
SpeciesMouse
TypeBasement Membrane Extract
StorageProduct is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 µg/ml gentamicin sulfate. Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines

Specifications/Features

Available conjugates: Sizes Available: 5 mL

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Multiple distinct metastatic cell states are induced by epithelial-mesenchymal plasticitybioRxivJuly 24, 2026Fahda Alsharief, Robert K. Suter, Apsra Nasir, Idalia Cruz, Gray W. Pearsonleast 16 h. The aggregates were then embedded in Matrigel (Corning, 354230) or Cultrex (Biotechne, 3533–005-02) and overlaid with Organoid Media (Supplementary file 1) in 6-well ultra-low adhesion plates (Sigma,

(2) Impact of non‐genetic heterogeneity of BRAF ‐mutant colon cancer organoids on growth kinetics, drug sensitivity and Wnt dynamicsInternational Journal of CancerJuly 15, 2026Viktoria Zieger, Ellen Woehr, Jasmin Traichel, Tilman Brummer, Roland Zengerle, Sabrina Kartmann, Stefan Zimmermannwere embedded in 4 mg/mL Cultrex BME (Reduced Growth Factor, Type 2, R&D Systems, Inc., USA; cat‐no. 3533‐005‐02) and seeded as 30 μL domes in 24‐well plates. For COCM+GF cultures, growth factors were also added to

(3) Distinct 2-phenylimidazo[1,2-a]pyridine derivatives that inhibit breast cancer cell proliferation identified as AHR ligandsiScienceJune 19, 2026Katrin Koellisch, Christine Blattner, Stefano Motta, Janine Wesslowski, Melanie Rothley, Simone Büchel, Savannah Sirounian, Ilenia Segatto, Hanna T. Weber, Julia Müller, et al.E1941 Collagenase,Merck Life Science,Cat# C9407 "Cultrex Basement Membrane Extract (BME), Type 2",R&D Systems,Cat# 3533-005-02 Cell recovery,Corning,Cat# 354253 Dulbeccoś phosphate buffered saline,Gibco,Cat# 14190094 "1,4-Dithiothreitol",Carl

(4) Tumor organoids modeling reveals timed responses and interplay of radiotherapy and chemotherapy in pancreatic cancerRadiotherapy and OncologyMay 1, 2026Chayu Yang, Zachery Keepers, Hem D. Shukla, Lei Ren5 min twice, and resuspended in Cultrex™ PathClear Reduced Growth Factor BME, Type 2 (R&D Systems, #3533–005–02). Organoids were then plated onto 24-well tissue culture plates (Costar, #3524) that had been pre-warmed

(5) Interferon-γ selectively promotes survival of alveolar progenitor cells in a human lung organoid modelThe EMBO JournalApril 16, 2026Antonella F M Dost, Katarína Balážová, Carla Pou Casellas, Lisanne M van Rooijen, Wisse Epskamp, Gijs J F van Son, Willine J van de Wetering, Carmen Lopez-Iglesias, Harry Begthel, Peter J Peters, et al.and cells were resuspended in a mixture of 20% washing buffer (Table EV1) and 80% BME (R + D Systems #3533-005-02) at a concentration of ~3000 cells/µl. Drops of 20–40 µL were plated on pre-warmed suspension culture

(6) Human airway organoids as a versatile model to study BSL-4 virus replication and pathogenesisSci RepMarch 27, 2026Joo-Hee Wälzlein, Sebastian Reusch, Jenny Ospina-Garcia, Ruth Olmer, Marc A. Schneider, Laura V. Klotz, Christian Klotz, Susann KummerBasement Membrane Extracts (BME, growth factor-reduced Type 2 basement membrane extract; R&D Systems CAT#3533-005-02) and 30% organoid medium. The cells were seeded as 70 µL BME droplet domes in 6-well plates, with each

(7) Protocol for generating an in vitro 3D multicellular culture model of ovarian high-grade serous carcinomaSTAR ProtocolsMarch 20, 2026Krister Wennerberg, Daria Bulanova, Laura Gall-Mas, Wojciech Senkowski, Lidia Moyano-Galceranproteins" ,, "Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear",R&D systems,Cat# 3533-005-02 Advanced DMEM/F-12 (1×),Thermo Fisher Scientific,Cat# 12634010 Primocin,InvivoGen,Cat# ant-pm-1 HEPES (1 M

(8) Spatial transcriptomics reveals altered communities and drivers of aberrant epithelia and pro-fibrotic fibroblasts in interstitial lung diseasesCell GenomicsMarch 11, 2026Alok Jaiswal, Tristan Kooistra, Vladislav Pokatayev, Hélder N. Bastos, Rita F. Santos, Tresa R. Sarraf, Åsa Segerstolpe, Crystal Lin, Liat Amir-Zilberstein, Shaina Twardus, et al.3335402001 "Cultrex Reduced Growth Factor Basement Membrane Extract, Type 2, Pathclear",R&D Systems,Cat # 3533-005-02 "Gibco™ TrypLE™ Select Enzyme (1X), no phenol red",Fisher Scientific,Cat # 50-591-420 Maxima H Minus

(9) The latest in vitro models for novel uveal melanoma drug discovery: how effective are they and what needs to be done?Expert Opin Drug DiscovMarch 10, 2026Lauren A. Dalvin, Stephanie D. Burr, Hien Ong, James A. ArmitageUM PDOs has been developed via culturing freshly dissociated tumor samples on Cultrex (R&D Systems 3533–005–02) (Figures 2 and 3) [91]. At least 40 unique PDOs have been established, and the PDOs accurately replicate

(10) Liver metastases dampen IL18-driven γδ T cell activity and immunotherapy responsiveness in colorectal cancerCell Reports MedicineFebruary 17, 2026Allard W.J. van Renterghem, Miguel Parra-Martinez, Maartje Witsen, Karlijn Verkerk, Marit Steur, Laurien J. Zeverijn, Krijn K. Dijkstra, Leila Akkari, Emile E. Voesttype IV (Sigma-Aldrich) and 10 μM Y-27632 (Sigma-Aldrich). Cells plated in Cultrex RGF BME type 2 (3533-005-02, R&D systems) and placed into a 37°C incubator for 20 min, and CRC organoid medium was added. The medium

(11) Regulatory grammar in human promoters uncovered by MPRA-based deep learningNatureFebruary 4, 2026Lucía Barbadilla-Martínez, Noud Klaassen, Vinícius H. Franceschini-Santos, Jérémie Breda, Hatice Yücel, Miguel Hernández-Quiles, Tijs van Lieshout, Carlos G. Urzua Traslaviña, Minh Chau Luong Boi, Maryam Akbarzadeh, et al.IV (Sigma-Aldrich) and 10 μM Y-27632 (Sigma-Aldrich). Cells were plated in Cultrex RGF BME type 2 (3533-005-02, R&D Systems) and placed in a 37 °C incubator for 20 min before adding CRC complete organoid medium.

(12) Glycolytic heterogeneity drives metabolic-targeted therapy in pancreatic ductal adenocarcinomaSignal Transduction and Targeted TherapyJanuary 20, 2026Ugo Chianese, Chiara Papulino, Gerardo Saggese, Ahmad Ali, Marianna Ciotola, Enza Lonardo, Mirko Cortese, Gregorio Favale, Annabella Di Mauro, Danila La Gioia, et al.each condition, 1000 cells were resuspended in 10 µL of basement membrane extract (BME2, R&D Systems, #3533-005-02) and plated as individual droplets in triplicate wells of a 96-well plate (Corning, #3596). The plate

(13) Microbial Metabolic Pathways Guide Response to Immune Checkpoint Blockade TherapyCancer discoveryJanuary 12, 2026Mimpen IL, Battaglia TW, Parra-Martinez M, Toner-Bartelds C, Zeverijn LJ, Geurts BS, Verkerk K, Hoes LR, van Renterghem AWJ, Noë M, et al.

(14) Crosstalk between H3K4me3 and oxidative stress is a potential target for the improvement of ART-derived embryosNature CommunicationsDecember 16, 2025Chuanxin Zhang, Hao Tian, Xiaoyu Yin, Xueqi Dong, Ning Wang, Yiwen Zhang, Jiawei Wang, Jinzhu Song, Cheng Li, Zi-Jiang Chen, et al.uniformly covered with 50 μL of Reduced Growth Factor Basement Membrane Extract (RGF BME), Type 2 (3533-005-02, R&D Systems), and the dishes were placed in a 5% CO2 incubator at 37 °C for 30 min. After the RGF BME

(15) Streptococcus mutans-derived extracellular vesicles promote herpes simplex virus infection in oral epitheliaVirulenceDecember 11, 2025Tae-Lyn Kim, Su Young Oh, Kah Young Lee, Heon-Jin Lee, Tae-Geon Kwon, Jin-Wook Kim, Chang-Geol Shin, Sungil Jang, Su-Hyung Hong, So-Young Choisamples were then centrifuged at 200 × g for 5 min at 4°C, and the pellets were resuspended in cold BME (3533–005-02; R&D Systems). Subsequently, 50-μL droplets were plated on the bottom of preheated suspension culture

(16) Targeting Interleukin‐6 as a Novel Strategy to Overcome Eribulin Resistance in Breast CancerCancer MedicineNovember 4, 2025Akira Hattori, Masayuki Nagahashi, Miki Komatsu, Sayaka Urano, Mamiko Kuroiwa, Yosuke Matsushita, Toyomasa Katagiri, Masafumi Shimoda, Yasuo MiyoshiCultrex Reduced Growth Factor Basement Membrane Extract (BME), Type 2 (10 mg/mL; Bio‐Techne, cat. no. 3533‐005‐02). Aliquots of 100 μL of the BME‐cell suspension were plated as small domes in each well of a prewarmed

(17) In vitro approaches to study centriole and cilium function in early mouse embryogenesisLife Sci AllianceOctober 29, 2025Isabella Voelkl, Tamara Civetta, Mirijam Egg, Marie Huber, Songjie Feng, Alexander Dammermann, Christa Bueckerresuspended in 20 μl ice-cold Cultrex Reduced Growth Factor Basement Membrane Extract (BME), Type 2 (3533-005-02; R&D Systems), which mimics the basement membrane that surrounds the epiblast during implantation (Bedzhov

(18) GABAergic signaling contributes to tumor cell invasion and poor overall survival in colorectal cancerOncogeneOctober 1, 2025Strelez C, Battaglin F, Perez R, Yang Y, Cherry C, Millstein J, Yoon AY, Chlystek JS, Canfield E, Haliday B, et al.

(19) Protocol for generation and utilization of patient-derived organoids from multimodal specimenSTAR ProtocAugust 18, 2025Sumin Kang, Mi Rim Lee, Wonyoung Choi, Sun-Young Kong, Yun-Hee KimFetal bovine serum,Gibco,16000044 "Cultrex reduced growth factor basement membrane extract, type 2, PathClear",R&D Systems,3533-005-02 "DNase I, RNase-free (1 U/μL)",Thermo Fisher Scientific,EN0521 Ficoll-Paque PREMIUM,Cytiva,17544202 "LIVE/DEAD viability/cytotoxicity kit, for mammalian cells

(20) Impact of 3D cell culture hydrogels derived from basement membrane extracts or nanofibrillar cellulose on CAR-T cell activationiScienceJuly 30, 2025Sonia Aristin Revilla, Alessandro Cutilli, Eugenia Cambiaso, Dedeke Rockx-Brouwer, Cynthia Lisanne Frederiks, Marc Falandt, Riccardo Levato, Onno Kranenburg, Caroline A. Lindemans, Paul James Coffer, et al.Recombinant murine FGF-basic,PeproTech,450-33 N-acetylcysteine,Sigma,A9165-5g "B-27™ Supplement(50X), serum-free",Gibco,17504044 "Cultrex RGF BME, Type 2",R&D systems,3533-005-02 "Matrigel, GFR",Corning,CLS356231-1EA GrowDex,UPM Biomedicals,100.103.905 GrowDase,UPM Biomedicals,100.103.905 Cell

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Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand

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