Fig 2: Angiopoietin‐like 4 in monolayer cultures of fibroblasts of the periodontal ligament stimulated with P. gingivalis lipopolysaccharide, E. coli lipopolysaccharide, IL‐1β, and TNFα in the presence of hypoxia or L‐mimosine. Human fibroblasts of the periodontal ligament (PDLF) in monolayer cultures were treated with P. gingivalis lipopolysaccharide (LPS Pg, A(i), B(i)), E. coli lipopolysaccharide (LPS Ec, A(ii), B(ii)), IL‐1β, A(iii), B(iii), TNFα, A(iv), B(iv), with and without the presence of hypoxia or L‐mimosine in medium without serum. Angiopoietin‐like 4 was measured at mRNA levels (Angptl4; A) and protein levels (ANGPTL4; B) using qPCR and ELISA, respectively. Bars represent mean + standard deviation, relative to the normoxic control. Experiments were performed twice with three different donors, respectively (N = 6). *P < 0.05 vs control (dashed line; A, white bar; B)

Fig 3: Hypoxia and L‐mimosine can increase angiopoietin‐like 4 in monolayer cultures of fibroblasts of the periodontal ligament. Human fibroblasts of the periodontal ligament (PDLF) in monolayer cultures were incubated in hypoxia or stimulated with L‐mimosine (L‐MIM) in medium with serum. Angiopoietin‐like 4 was measured at mRNA level (Angptl4; A) and protein level (ANGPTL4; B) using qPCR and ELISA, respectively. Bars represent mean + standard deviation, relative to the normoxic control. Experiments were performed three times with two different donors, respectively (N = 6). *P < 0.05 vs control (dashed line; A, white bar; B)