Recombinant Human TNF-alpha, Biotinylated Protein BT210-010 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human TNF-alpha, Biotinylated Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $388.00Supplier Page View Company Product Page
Catalog NumberBT210-010
Size10 ug
ApplicationsBioactivity
Conjugate/TagBiotin
FormatLyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein
Purity>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Molecular WeightObserved: 17 kDa, reducing conditions
Theoretical: 17 kDa (unlabeled)
SpeciesHuman
Molecule NameTNF-alpha
Source/Expression SystemE. coli-derived Recombinant Human TNF-alpha, Biotinylated Protein
NCBI Full Gene Nametumor necrosis factor
NCBI Gene AliasesTNFSF2, DIF, TNF-alpha, TNLG1F, TNFA
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Inhibition of TBK1/IKKε mediated RIPK1 phosphorylation sensitizes tumors to immune cell killingCell Death DiscoveryNovember 28, 2025Anastasia Piskopou, David W. Vredevoogd, Xiangjun Kong, Daniel S. Peeper, Maarten Altelaar, Kelly E. Steckeror sgTBK1/IKKε) were cultured for 24 h and subsequently incubated with 100 ng/mL biotinylated TNF (BT210–010, R&D Systems) or unlabeled TNF (300-01 A, PeproTech) for 10 min. Following this treatment, cells were

(2) Targeted protein degradation through site-specific antibody conjugation with mannose 6-phosphate glycanmAbsDecember 31, 2024Kaori Mukai, Robert Cost, Xin Sheen Zhang, Emily Condiff, Joanne Cotton, Xiaohua Liu, Ekaterina Boudanova, Björn Niebel, Peter Piepenhagen, Xinming Cai, et al.antibodies are specified in the methods described for characterization. Biotinylated TNF (catalog number as BT210–010) was purchased from R&D systems, while Alexa Fluor™ 647-labeled streptavidin (catalog number as S32357)

(3) Biodistribution and function of coupled polymer-DNA origami nanostructuresScientific reportsNovember 10, 2023Joseph N, Shapiro A, Gillis E, Barkey S, Abu-Horowitz A, Bachelet I, Mizrahi B.

(4) RNF31 inhibition sensitizes tumors to bystander killing by innate and adaptive immune cellsCell reports. MedicineJune 21, 2022Zhang Z, Kong X, Ligtenberg MA, van Hal-van Veen SE, Visser NL, de Bruijn B, Stecker K, van der Helm PW, Kuilman T, Hoefsmit EP, et al.addition of HOIPIN-8 (20 μM) or vehicle for 24 h before treatment with 100 ng/mL biotinylated TNF (BT210–010, R&D Systems) or unlabeled TNF (Peprotech) for 10 min. Cells were then collected and lysed in IP lysis

(5) Tumor necrosis factor receptor-1 is selectively sequestered into Schwann cell extracellular vesicles where it functions as a TNFα decoyGliaFebruary 1, 2022Sadri M, Hirosawa N, Le J, Romero H, Martellucci S, Kwon HJ, Pizzo D, Ohtori S, Gonias SL, Campana WM.described (Crookston & Gonias, 1994). Rat SC EVs (10 μg) were incubated with TNFα (0.5 nM, R&D Systems, BT210–010) and then with 5 mM BS3 for 10 min at 37°C. TNFα (0.5 nM) was treated with BS3 in the absence of SC

(6) Roles for ADAM17 in TNF-R1 Mediated Cell Death and Survival in Human U937 and Jurkat CellsCellsNovember 10, 2021Fritsch J, Frankenheim J, Marischen L, Vadasz T, Troeger A, Rose-John S, Schmidt-Arras D, Schneider-Brachert W.

(7) Regulation of CYLD activity and specificity by phosphorylation and ubiquitin-binding CAP-Gly domainsCell reportsOctober 5, 2021Elliott PR, Leske D, Wagstaff J, Schlicher L, Berridge G, Maslen S, Timmermann F, Ma B, Fischer R, Freund SMV, et al.

(8) Soft extracellular matrix enhances inflammatory activation of mesenchymal stromal cells to induce monocyte production and traffickingScience advancesApril 1, 2020Wong SW, Lenzini S, Cooper MH, Mooney DJ, Shin JW.

(9) ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptorThe Journal of biological chemistryFebruary 2, 2018Holdbrooks AT, Britain CM, Bellis SL.

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TNF-alpha Biotinylated Protein

TNF-alpha Biotinylated Protein

TNF-alpha Biotinylated Protein

Recombinant Human TNF-alpha, Biotinylated Protein from R&D Systems, a Bio-Techne Brand

R&D Systems, a Bio-Techne Brand

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Recombinant Human TNF-alpha, Biotinylated Protein from R&D Systems, a Bio-Techne Brand

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