Fig 1: UNO specificity for CD206. (a) Change in fluorescence anisotropy of FAM-UNO (dotted line) and FAM-UNO in DTT (solid line) while incubating with mouse recombinant CD206. (b) Change in fluorescence anisotropy of FAM-CSPGAK with mouse recombinant CD206 (solid line) or with CD163 (dotted line) and of FAM-CPMTDNE (control) with CD206 (dashed line). (c) FAM-UNO binds selectively to CCR2+ macrophages collected from the peritoneal cavity of 4T1 tumor-bearing mice, as 94.3% of FAM+ cells are CCR2+ cells. The analysis was done gating for the FAM+ population (left panel). In these 4T1 tumor bearing mice, 58% of peritoneal cells are macrophages, i.e CCR2+ cells (right panel). (d) FAM-UNO binding to peritoneal cells is inhibited by preincubating with 10 µg/mL of anti-CD206, whereas the preincubation with anti CD206 antibody had no effect on FAM-LyP-1 binding. In panels a and d are shown representative graphs from three independent experiments. In panels c and d are shown results from three independent experiments (n = 3 mice) and bars of panel d represents mean + SEM.