Cultrex RGF Basement Membrane Extract, Type 2 3533-010-02 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCultrex RGF Basement Membrane Extract, Type 2
CompanyR&D Systems, a Bio-Techne Brand
Price $455.00Supplier Page View Company Product Page
Catalog Number3533-010-02
Size10 mL (2 x 5 mL)
ApplicationsBioactivity
Format12 - 18 mg/mL
TypeBasement Membrane Extract
StorageProduct is stable for a minimum of 3 months from date of shipment when stored at -20 °C in a manual defrost freezer. For optimal stability, store at -80 °C. Avoid freeze-thaw cycles. Storage Buffer: Dulbecco’s Modified Eagle’s medium without phenol red, with 10 ug/ml gentamicin sulfate. Sterility Testing: PathClear® - Negative by PCR test for mycoplasma; 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses. No bacterial or fungal growth detected after incubation at 37 °C for 14 days following USP sterility testing guidelines

Add to Compare List

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) WNT7B drives a program for pancreatic cancer subtype switching and progressioniScienceMarch 20, 2026Joep Sprangers, Jeroen M. Bugter, Despina Xanthakis, Michiel Boekhout, Rutger N.U. Kok, Veerle E. Geurts, Hans Clevers, Lodewijk A.A. Brosens, Frederike Dijk, Maria J. Rodríguez Colman, et al.bromide),Merck,3701-03-3 Puromycin,Santa Cruz,SC-108071 Matrigel,Corning,356231 Basement Membrane Extract (BME),R&D Systems,3533-010-02 (RGF) TrypLE,Thermo Fisher Scientific (Life Technologies),12604-021 Advanced DMEM/F12,Invitrogen,12634010 Penicillin-Streptomycin,Lonza,17-602F HEPES,Thermo

(2) NSUN2 mediates intestinal stem cell expansion and colorectal tumour initiation via MAPK/ERK signallingCell Death & DiseaseMarch 19, 2026Aslihan Bastem Akan, Caroline V. Billard, Szu-Ying Chen, Po-Hsien Huang, Patrizia Cammareri, Vidya Rajasekaran, Adam E. Hall, Paula Preyzner, Sebastian Öther-Gee Pohl, Susan M. Farrington, et al.varying between 3 and 5 days, and cultured in Cultrex PathClear Reduced Growth Factor BME (Bio-Techne, 3533-010-02). The plate was incubated for 10 min at 37 °C to allow BME solidification, and 500 µl of growth medium

(3) CRISPR-engineered human lung organoids with a biomolecular condensate reporter enable mechanistic toxicity monitoringMaterials Today BioFebruary 26, 2026Seung-Yeon Kim, Ji-Won Baek, Eo Jin Kim, Sathiyaraj Srinivasan, Kee K. Kim, Eun-Mi KimNational University, Seoul, Korea). LCOs were embedded in reduced growth factor basement membrane extract (3533-010-02, R&D systems, Minneapolis, MN, ULA) and maintained in the organoid culture medium supplied by KCLB following

(4) Ex Vivo Immuno-Oncology Platform Reveals Spatial T-cell Infiltration Patterns Linked to ATR Inhibition Responses in High-Grade Serous Ovarian CancerCancer Immunology ResearchFebruary 25, 2026Ashwini S Sakrepatna Nagaraj, Matilda Salko, Aditi Sirsikar, Ziqi Kang, Erdogan Pekcan Erkan, Elina A. Pietilä, Iga Niemiec, Jie Bao, Giovanni Marchi, Angéla Szabó, et al.HGSC tumor-dissociated cells were mixed with Cultrex RGF Basement Membrane Extract (BME), Type 2 (# 3533-010-02 R&D Systems), or 2.5 mg/mL OmGel at a concentration of 1000 cells / μL and plated as a droplet in 48-well

(5) Targeting cancer stem cells predicts response and reverses chemoresistance in ascites-derived ovarian cancer organoidsBMC MedicineFebruary 24, 2026Xiaoli Zhang, Wen Zhang, Ying Cui, Qi Zhang, Duo Wan, Ning Li, Kaitai Zhang, Huiqin Guothen mixed with Culture Reduced Growthfactor Basement Membrane Extract (BME) (R&D Systems, cat. no. 3533–010-02) to a final BME concentration of 70% BME. Approximately 40,000 cells or cell clusters were embedded

(6) Long-term feeder cell-free cat intestinal organoid cultures to study Toxoplasma gondii’s sexual developmentCommunications BiologyFebruary 14, 2026David Warschkau, Tobias Hoffmann, Michael Laue, Antonia Müller, Chandra Ramakrishnan, Giulia Rigamonti, Fabrizia Veronesi, Elvio Lepri, Mohamed Ali Hakimi, Christian Klotz, et al.resuspended in Advanced DMEM/F12 and mixed 1:1 with Cultrex basement membrane extract (R&D Systems, Cat. No. 3533-010-02). 50 µl droplets were placed into wells of a 24-well plate and hardened at 37 °C before adding cat organoid

(7) Activating KRAS mutations mark premalignant cystic structures in congenital pulmonary airway malformationsRespiratory ResearchJanuary 15, 2026Cathy van Horik, Floor Benthem, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Wilfred .F.J. van IJcken, Rene .M.H. Wijnen, Marco. Schnater, Robbert .J. Rottierbuffer, the cells where plated in Culturex Basement Membrane Extracts type 2 (BME) (Bio-techne, cat nr 3533-010-02) at a density of 1.000.000 cells per drop of 30 µL BME. Airway Organoid medium (AO medium) with cefuroxime

(8) Liquid Metal Microrobots for Magnetically Guided Transvascular NavigationAdvanced MaterialsDecember 19, 2025Xiaohui Ju, Roshan Velluvakandy, Xianghua Wu, Miguel Angel Merlos Rodrigo, Zbyněk Heger, Kamila Bendíčková, Jan Frič, Martin Pumera(Sigma–Aldrich # F1141‐5MG, 5 µg cm−2 in phosphate buffered saline, PBS), Cultrex (R&D systems # #: 3533‐010‐02, diluted 100× in PBS), or Matrigel (Corning # 356 231, diluted 100× in PBS). A volume of 50 µL coating

(9) Defining the Antitumor Mechanism of Action of a Clinical-stage Compound as a Selective Degrader of the Nuclear Pore ComplexCancer discoveryDecember 2, 2025Yuan L, Ji W, Dwyer BG, Lu J, Bian J, Colombo GM, Martinez MJ, Fernandez D, Phillips NA, Tang MT, et al.

(10) Patient-Derived Organoids Predict Treatment Response in Metastatic Colorectal CancerClinical Cancer ResearchDecember 1, 2025Lidwien P. Smabers, G. Emerens Wensink, Carla S. Verissimo, Mayke Doorn, Tao Yang, Timo Voskuilen, Maarten A. Huismans, Liselot B.J. Valkenburg-van Iersel, Geert A. Cirkel, Elske C. Gootjes, et al.PDOs were resuspended in organoid screening medium with Y-27632 with 5% basement membrane extract (#3533-010-02, Bio-Techne) for a final 125,000 PDOs/20 mL concentration. Drug screening was performed as described

(11) Impact of treatment history on drug resistance of metastatic colorectal cancer organoidsiScienceNovember 21, 2025Maarten A. Huismans, Lidwien P. Smabers, Sascha R. Brunner, Arne van Hoeck, Demi van de Kaa, Ingrid A. Franken, Emerens Wensink, Jan Koster, Richard Volckmann, Onno Kranenburg, et al.(adDMEM+++) and re-plated by embedding in a solution of ice-cold BME (Bio techne, Dublin, Ireland, #3533-010-02) and plated as droplets on a pre-warmed 6-well plate. After solidification of the BME, organoid culturing

(12) The Phenotypical and Functional Effect of PGE2 on Human MacrophagesEuropean Journal of ImmunologyNovember 10, 2025Maren Pfirrmann, Johanna Bödder, Rowan Wuestenenk, Jesse Tennebroek, Kirti K. lyer, Dennis Poel, Daniele V.F. Tauriello, Martijn Verdoes, I. Jolanda M. de Vriesin 12 µL domes of Cultrex Reduced Growth Factor Basement Membrane Extract Type 2 (BME) (Bio‐Techne, 3533‐010‐02) in six‐well cell culture plates with 2 mL culture medium. As culture medium, we prepared +3 medium

(13) Benchmarking porcine pancreatic ductal organoids for drug screening applicationsEMBO Molecular MedicineNovember 4, 2025Christos Karampelias, Kaiyuan Yang, Falk J Farkas, Michael Sterr, Mireia Molina van Den Bosch, Simone Renner, Janina Fuß, Christine von Toerne, Sören Franzenburg, Tatsuya Kin, et al.Y-27632 dihydrochloride,Sigma-Aldrich,Y0503-1MG Nicotinamide,Sigma-Aldrich,N0636-100G N-acetyl-L-cysteine,Sigma-Aldrich,A9165-5G 3dGRO L-WRN,Sigma-Aldrich,SCM105 Cultrex RGF basement membrane extract Type 2,Bio-Techne,3533-010-02 Porcine serum,Sigma-Aldrich,P9783-500ML DAPT,Tocris Bioscience,2634 4-Diethylaminobenzaldehyde,Sigma-Aldrich,D86256 BMS-536924,Sigma-Aldrich,TA9947273392 Mouse

(14) A Novel Human Distal Tubuloid-on-a-Chip Model for Investigating Sodium and Water Transport MechanismsKidney360November 1, 2025Murillo D.L. Bernardi, Emre Dilmen, Dorota Kurek, Henriette L. Lanz, Jos Joore, Joost G. Hoenderop, Maarten B. Rookmaaker, Marianne C. Verhaarexperiments. Tubuloids were embedded in Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, 3533-010-02) and cultured in expansion medium (EM) composed of advanced modified eagle medium/nutrient mixture F12

(15) Mechanosensitive calcium channels and integrins coordinate the reprogramming of colorectal cancer cells into a fetal-like stateCell reportsOctober 28, 2025van der Net MC, Vliem MJ, Kemp LJS, Perez-Gonzalez C, Haddad TS, Strating EA, Krotenberg-Garcia A, Houtekamer RM, Pannekoek WJ, van den Anker KB, et al.

(16) TCR-engineered T cells targeting a shared β-catenin mutation eradicate solid tumorsNature immunologyOctober 1, 2025Eggebø MS, Heinzelbecker J, Palashati H, Chandler N, Tran TT, Li Y, Yang W, Laos M, Blaas I, Rustad EH, et al.

(17) EMT-ciliary signaling in quasi-mesenchymal-stem-like cells drives therapeutic resistance and is a druggable vulnerability in triple-negative breast cancerEMBO molecular medicineOctober 1, 2025Tessier CE, Derrien J, Dupuy AMM, Pelé T, Moquet M, Roul J, Douillard E, El Harrif C, Pinson X, Le Gallo M, et al.protein,Thermo-Fisher,120-10C-100UG B-27,Thermo-Fisher,17504044 Nicotinamide,Sigma,n0636-100g N-acetyl-L-cystein,Sigma,A9165 Primocin,Invivogen,ant-pm-05 Y27-632,AbMole,M1817 Heregulin,Peprotech,AF-100-03 FGF-7,Peprotech,AF-100-19 FGF-10,Peprotech,AF-100-26 A83-01,Sigma,SML0788-5MG EGF,Peprotech,AF-100-15 SB202190,Sigma,S7067-5MG BME,Bio-Techne,3533-010-02 Formaldehyde,Thermo-Fisher,11586711 Triton X-100,VWR,437002A Normal Serum Block,Biolegend,927503 Tween-20,Thermo-Fisher,10113103 Hoechst,Invitrogen,H3570 RNA

(18) BRD2 Upregulation as a Pan-Cancer Adaptive Resistance Mechanism to BET InhibitionbioRxivSeptember 11, 2025Suyakarn Archasappawat, Juliette Jacques, EunJung Lee, Chang-il Hwang15–1040), and resuspended in 50 μl of Cultrex Reduced Growth Factor Basement Membrane Extract (Bio-Techne, 3533-010-02). Each mouse received 0.5 × 106 cells: shScr control cells in the left flank and shBrd2 cells in the

(19) Tumor nutrient stress gives rise to a drug tolerant cell state in pancreatic cancerbioRxiv : the preprint server for biologySeptember 8, 2025Sheehan C, Hu L, Cognet G, Croley G, Nguyen TT, Thomas-Toth A, Agovino D, Jonker PB, Sadullozoda M, Ziolkowski LM, et al.

(20) Microbial riboflavin inhibits ceramide synthase 3 to lower ceramide (d18:1/26:0) and delay colorectal cancer progressionCell MetabolismSeptember 2, 2025Ruize Qu, Yi Zhang, Bora Kim, Guangyi Zeng, Pengcheng Wang, Weike Shaoyong, Ying Huang, Wanwan Guo, Yang Chen, Ping Wang, et al.filtration through a 70 μM filter, cells were centrifuged again and resuspended in BME (R&D Systems, #3533–010-02). The single-cell suspension was then dispensed into a 96-well plate at approximately 3000 cells per

Be the First to Write a Review!

Cultrex RGF Basement Membrane Extract, Type 2

Cultrex RGF Basement Membrane Extract, Type 2

Cultrex RGF Basement Membrane Extract, Type 2

Cultrex RGF Basement Membrane Extract, Type 2

Cultrex RGF Basement Membrane Extract, Type 2

Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand

Description

R&D Systems, a Bio-Techne Brand

Citations (149)

Reviews

Cultrex RGF Basement Membrane Extract, Type 2 from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map