Corning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL 354277 from Corning Life Sciences

Product Specs
ItemCorning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL
CompanyCorning Life Sciences
Price $224.99Supplier Page View Company Product Page
Catalog Number354277
Size1 / Pk
Source/Expression SystemEngelbreth-Holm-Swarm (EHS) mouse tumor
TypeExtracellular Matrices

Specifications/Features

Size: 5 mL

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Corning Life Sciences

836 North Street

Building 300 Suite 3401

Tewksbury, MA 01876

United States

Phone: (978) 442-2200
Phone: (800) 492-1110

Email: clscustserv@corning.com

Company Profile

Website: www.corning.com/lifesciences

(1) Application of spatial transcriptomics across organoids for a high-resolution, spatial whole-transcriptome benchmarking datasetiScienceJune 19, 2026Maria Rosaria Nucera, Natalie Charitakis, Ryan F. Leung, Anna Leichter, Natasha K. Tuano, Marzena Walkiewicz, Vallari Sawant, Lynn Rowley, Michelle Scurr, Pei X. Er, et al.brain organoids were grown on human embryonic stem cell (hESC)-qualified Matrigel Matrix (Corning Cat# 354277), with varying medium conditions. iPSCs for brain organoids were grown on Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix

(2) Protocol for generating iPSC-derived intestinal organoids through dissection to model cystic fibrosis therapiesSTAR ProtocolsJune 1, 2026Alexander J. Stuffer, Barbara Tabak, Joshua Conte, Victoria Momyer, Mercy Chado, Matthew Armstrong, Phillip Wible, Andrey Sivachenko, Normand Allaire, Junjie Lu, et al.,Stem Cell Technologies,100–0276 ReLeSR™,Stem Cell Technologies,100–0484 "Corning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL",Corning,354277 "Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free, 10 mL

(3) Naïve pluripotency and genomic stability are coordinated in embryonic stem cells by a novel pluripotency regulator ZFP998Nucleic Acids ResMay 27, 2026Min Tang, Lin Wang, Jia-Wen Liu, Wan-Huan Zhong, Han Wu, Wen-Hui Nie, Guo-Meng Li, Wei-Dao Zhang, Hu Zhou, Jing Gao, et al.XAV939 (Sigma, X3004). H9 hESCs were cultured in Essential 8 medium on Matrigel-coated dishes (Corning, 354277). All cells were maintained at 37°C in a humidified incubator with 5% CO2. For the resetting of EpiSCs

(4) Microglial MARCO facilitates Varicella zoster virus uptake and triggers TLR2-mediated neuroinflammationJ Biomed SciMay 23, 2026Ji-Soo Lim, Soo-Jin Oh, Ji-Yeun Hur, Subin Oh, Seuk-Min Ryu, Rafael T. Han, Hosun Park, Dawn M. E. Bowdish, Ok Sarah Shinprotocol with minor modifications [19]. Briefly, undifferentiated ESCs were maintained in Matrigel (354277, Corning)-coated dishes in Essential 8 medium (A1517001, Gibco) until they reached 80–90% confluency.

(5) Multilayered microelectrode array for monitoring electrophysiological signals of 3d neural networks in cerebral organoidMicrosyst NanoengMay 22, 2026Namyun Kim, Minjin Kang, Joonhwan Ji, Hyelim Kim, Hong Nam Kim, Nakwon Choi, Yi Jae LeeKIST-202305-BR-002). Culture plates were coated with Corning® Matrigel® hESC-Qualified Matrix, LDEV-free (5 mL; Cat. #354277; Corning, USA) diluted 1:25 in DMEM/F-12 (Cat. #11320033; Gibco, USA). Cells were cultured in mTeSR™1

(6) Multispectral extended depth-of-field fluorescence microscopy with co-designed meta-optics and neural reconstructionLight Sci ApplMay 19, 2026Ipek Anil Atalay Appak, Haobijam Johnson Singh, Sanna Korpela, Teemu O. Ihalainen, Erdem Sahin, Christine Guillemot, Humeyra Caglayanempty lumens instead of a monolayer. Cells were cultured and passaged as described above. Matrigel (#354277, Corning) was thawed overnight on ice and kept below 4 ∘C prior to gelation. Cells were mixed with Matrigel,

(7) Mannose-6-Phosphate-Tagged Liposomes Exhibit Increased Transcytosis Across Human Blood-Brain Barrier ModelPharmaceuticsMay 19, 2026Margarita C. Dinamarca, Boris Sevarika, Scott McNeilwere cultured in Matrigel-coated dishes (according to the manufacturer’s recommendations, Cat. No. 354277, Corning, New York, NY, USA) in mTeSR Plus medium (Cat. No. 100-0276, STEMCELL Technologies, Vancouver,

(8) Modeling human enterovirus A71 infection using an intestinal microphysiological systemJournal of VirologyMay 19, 2026Hiroki Futatsusako, Sayaka Deguchi, Kaori Kosugi, Rina Hashimoto, Noriyo Nagata, Tadaki Suzuki, Takuya Yamamoto, Kazuo TakayamaFUJIFILM Wako Pure Chemical). Human ES cells were also maintained on Matrigel hESC-Qualified Matrix (Cat# 354277, Corning) with mTeSR1-cGMP medium (Cat# ST-85850, STEMCELL Technologies). For passaging, human ES cells

(9) Morphine potentiates HIV infection and receptor expression in 3d brain organoidsJ NeurovirolMay 18, 2026Michael Swingler, Doga Tasdemir, Senem Cakir, Anna Bellizzi, Martina Donadoni, Ilker K. SariyerCLS3473) containing induction Medium. On day 8, each EB was embedded in 15 µL of Matrigel® (CORNING, 354277) on organoid embedding sheets (STEMCELL TECHNOLOGIES, 08579). After Matrigel was allowed to polymerize,

(10) Optimizing post-transplantation detection of subcutaneously transplanted islets using dithizone stainingBMC MethodsMay 18, 2026James Lu, Matthew Ishahak, Marlie M. Maestas, Jeffrey R. Millmanpublished protocol [8]. Briefly, undifferentiated stem cells were seeded onto a Matrigel (Corning; 354277) treated tissue culture flask at 0.63 × 106 cells/cm2 with mTesR1 (StemCell Technologies; 05850) and

(11) Non-invasive in vivo monitoring of transplanted human intestinal organoids using bioluminescenceSurg Open SciMay 15, 2026Chioma Moneme, Antonio Vinicios Alves da Silva, Prisca C. Obidike, Bryan A. Hogg, Georgia B. Brousseau, Casandra Robinson, Christian Roig-Laboy, José Kleybson de Sousa, Yuwen Zhang, Lily S. Cheng, et al.cells were cultivated in the 6-wells-plate previously coated with hESC qualified Matrigel™ (Corning, #354277) and fed with mTeSR1™ Complete Medium until colonies reach about 80% confluence. Cells were arrested

(12) A long-read human pangenome initiative for comprehensive interpretation of nuclear-embedded mitochondrial DNANat CommunMay 15, 2026Lianting Fu, Jieyi Chen, Da Lian, Siyuan Du, Dongya Wu, Chentao Yang, Ziyi Wang, Hongyi Ma, Zhengtong Li, Nicole J. Lake, et al.post-transfection. For fluorescence imaging, cells were replated onto coverslips coated with Matrigel (Corning, #354277) on Day 2 post-transfection and imaged on Day 3 post-transfection. RT-qPCR Total RNA was extracted

(13) Reduced LINC01089 Expression Impairs Coordinated Heme-Globin Transcriptional Programs in Human Erythroid CellsInt J Mol SciMay 14, 2026Yuan Xue, Xiaohong Li, Yonggang Zhang, Feng Mato the manufacturer’s instructions. H1 cells were maintained on Matrigel (Corning, Corning, NY, USA; 354277)-coated plates in NcTarget™ hPSC Medium (Shownin Biotechnologies Co., Ltd., Hefei, China), with daily

(14) MECP2 mutations rewire human ESC fate and bias cortical lineage commitmentStem Cell ReportsMay 12, 2026Marion Guillon, Margaux Brin, Elodie Gabet, Justine Gromaire, Mathéa Bernard, Laetitia Laurent, Théo Rabin, Lisa Bianchin, Marie Veziano, Julie Kloda, et al.culture, cells were thawed onto tissue-culture plates coated with hESC-qualified Matrigel (Corning, #354277) and maintained in mTeSR Plus medium (Basal Medium #100-0274 supplemented 1:5 with mTeSR Plus 5× Supplement,

(15) Biomimetic microfibers for myelin-enhancer screening and neural regenerationCyborg and Bionic SystemsMay 7, 2026Lili Quan, Akiko Uyeda, Atsushi Sekiguchi, Ze Zhang, Kazuhisa Sakai, Tsunehiko Takamura, Ruijuan Zhang, Noritaka Ichinohe, Shinjiro Umezu, Rieko Muramatsu[18]. Cells were cultured in mTeSR1 medium with Y27632 (030-24021, Fuji film) and plated on Matrigel (354277, Corning)-precoated 6-well plate at a density of 1.5 × 105 to 2 × 105 cells. After 24 h, the culture

(16) Generation of Functional Patient-Specific Thymus Organoids From Human Pluripotent Stem Cells (hPSCs) Using Air–Liquid Interface CultureBio ProtocMay 5, 2026Stephan A. Ramos, Holger A. RussL0288-100 mL) 40. LY364947 (R&D Systems, catalog number: 2718) 41. Matrigel (Corning, catalog number: 354277) 42. Methyl cellulose (Sigma-Aldrich, catalog number: M7027-100G) 43. mTeSR Plus (STEMCELL Technologies,

(17) Inhibition of Adenosine Kinase Alleviates Hypertrophic Cardiomyopathy by Ameliorating Coronary Microvascular DysfunctionESC Heart FailureMay 5, 2026Fangfang Wan, Bingxuan Ma, Ziqiang Han, Youyang Zheng, Minjie Lu, Jingzhou Chen, Jizheng Wang, Lei Songformation HCMECs (60 000 cells per well) were seeded in 96-well plates coated with growth factor-reduced Matrigel (354277, Corning, USA). Cells were photographed under a microscope 6 h after growth on Matrigel. Tube formation

(18) Engineering of functional auditory neurons from human induced pluripotent stem cellsMil Med ResMay 1, 2026Minjin Jeong, Lucas G. Vattino, Maja Djurisic, Kevin P. Rose, Hiroshi Hyakusoku, Svetolik Spasic, Xiao-Jie Ma, Hiroaki Mohri, Ronna Hertzano, Anne E. Takesian, et al.number 50. Cells were maintained on Matrigel human embryonic stem cell (hESC) qualified matrix (Cat# 354277, Corning, USA) in mTeSR1 (Cat# 85850, StemCell Technologies, Canada) or mTeSR plus medium (Cat# 100-0276,

(19) Novel role of the lncRNA EPR as oncosuppressor in intestinal cancerbioRxivMay 1, 2026Naomi Shim, Maira Rossi, Monica Nicolau, Jesus R Barajas, Ettore Zapparoli, Paola Briata, Pier L Puri, Roberto Gherzi, Luca CaputoWA09) were maintained in mTESR+ media (Stem Cell Technologies Cat #100-0276) on Matrigel® (Corning Cat #354277) coated dishes in incubator at 37°C, 5% CO2. H9 cells were differentiated to small intestinal organoids

(20) Isoginkgetin protects against degeneration of ALS motor neurons via regulating the GSK-3β–TFEB signaling axisPharmacological ResearchMay 1, 2026Ang Li, Xianglu Xiao, Guopan Liu, Krinos Li, Yixia Ling, Shenglong Deng, Chunzuan Xu, Shu-qin Cao, Jing Wen, Guang Lu, et al.TARDBP A315T mutation into the healthy GZF2-iPSC line. All the iPSC cells were cultured in Matrigel (354277; Corning)-precoated cell culture plastic plates or confocal dishes in mTeSR Plus basal medium (100–0276;

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7/25/2021Great Matrigel Matrix for Ips Cells Xiaoguang LiWe used this Corning® Matrigel® hESC-Qualified Matrix to coat the 6-well plate for ips cells culture.

Corning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL

Corning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL

Corning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL from Corning Life Sciences

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Corning Life Sciences

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Corning® Matrigel® hESC-Qualified Matrix, LDEV-free, 5 mL from Corning Life Sciences

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