Alexa Fluor® 594 Streptavidin 405240 from BioLegend

Product Specs
ItemAlexa Fluor® 594 Streptavidin
CompanyBioLegend
Price $265.00Supplier Page View Company Product Page
Catalog Number405240
Size100 µg
ApplicationsICC - Quality tested, IHC-F - Verified, IHC-P
Concentration0.5 mg/mL (concentration relates to the Streptavidin only component of the conjugate)
Conjugate/TagAlexa Fluor® 594
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
SpeciesHuman, Mouse, Rat, All Species
TargetBiotin
TypeStreptavidin

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Migration arrest and transendothelial trafficking of human pathogenic-like Th17 cells are mediated by differentially positioned chemokinesNature communicationsFebruary 26, 2025Parween F, Singh SP, Kathuria N, Zhang HH, Ashida S, Otaizo-Carrasquero FA, Shamsaddini A, Gardina PJ, Ganesan S, Kabat J, et al.washing with Vascular Cell Basal Medium, cells were incubated with Alexa Fluor 594 streptavidin (1:500, 405240; BioLegend) for an additional 30 min. Cells were washed with Vascular Cell Basal Medium once followed

(2) B Cell Activation, Differentiation, and Their Potential Molecular Mechanisms in Osteoarthritic Synovial TissueJournal of inflammation researchJanuary 1, 2025Lu P, Li Y, Yang S, Yao H, Tu B, Ning R.sections were then washed and incubated with Alexa 594-conjugated secondary (Biolegend USA Cat no: 405240) antibodies or Alexa 488-conjugated antibodies (Biolegend USA Cat no: 405235) at room temperature for

(3) mRNA-LNP HIV-1 trimer boosters elicit precursors to broad neutralizing antibodiesScienceMay 17, 2024Zhenfei Xie, Ying-Cing Lin, Jon M. Steichen, Gabriel Ozorowski, Sven Kratochvil, Rashmi Ray, Jonathan L. Torres, Alessia Liguori, Oleksandr Kalyuzhniy, Xuesong Wang, et al.,BD Bioscience,563259 Alexa Fluor 488-Streptavidin,BioLegend,405235 Alexa Fluor 594-Streptavidin,BioLegend,405240 Sigma Adjuvant,Sigma,S6322 ACK lysing buffer,Lonza,BP10–548E

(4) Transcription factor Tox2 is required for metabolic adaptation and tissue residency of ILC3 in the gutImmunityMay 14, 2024Arundhoti Das, Gustavo Ulises Martinez-Ruiz, Nicolas Bouladoux, Apollo Stacy, Josquin Moraly, Maria Vega-Sendino, Yongge Zhao, Marieke Lavaert, Yi Ding, Abigail Morales-Sanchez, et al.Antibody (RA3-6B2), APC",ThermoFisher Scientific,17-0452-82 Alexa Fluor® 594 Streptavidin,Biolegend,405240 Recombinant Anti-Hexokinase 1 antibody [EPR10134(B)],abcam,ab150423 Recombinant Anti-Hexokinase II

(5) A modified SELEX approach to identify DNA aptamers with binding specificity to the major histocompatibility complex presenting ovalbumin model antigenRSC advancesOctober 31, 2023Lin Y, Chen CY, Ku YC, Wang LC, Hung CC, Lin ZQ, Chen BH, Hung JT, Sun YC, Hung KF.30 minutes followed by staining with the 1 : 500 secondary antibody Streptavidin Alexa-594 (Cat. no.405240, BioLegend, USA) at 4 °C for 30 minutes. Nuclei were stained with 300 nM DAPI at 4 °C for 15 minutes.

(6) T Cells Expressing a Modified FcγRI Exert Antibody-Dependent Cytotoxicity and Overcome the Limitations of CAR T-cell Therapy against Solid TumorsCancer immunology researchJune 2, 2023Rasoulouniriana D, Santana-Magal N, Gutwillig A, Farhat-Younis L, Tal L, Amar S, Milyavsky M, Muddineni SSNA, Solomon N, Shpilt H, et al.Human HER2/ErbB2 Protein (ECD) Biotinylated (Sino Biological, #10004-HCCH-B) and Alexa Fluor 594 Streptavidin (BioLegend, #405240). Images were collected using a Zeiss LSM800 confocal laser scanning microscope using Objective Plan-Apochromat

(7) Altered Tau Kinase Activity in rTg4510 Mice after a Single Interfaced CHIMERA Traumatic Brain InjuryInternational journal of molecular sciencesMay 29, 2023Cheng WH, Cheung H, Kang A, Fan J, Cooper J, Anwer M, Barron C, Wilkinson A, Hu G, Yue J, et al.blocked, and probed with p62 antibody (NEB D5L7G, 1:800, Ipswich, MA, USA) and streptavidin-594 (Biolegend 405240, 1:500, San Diego, CA, USA), using the Mouse on Mouse kit (Vector BMK2202, Newark, USA) per the manufacturer’s

(8) Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cellsbioRxiv : the preprint server for biologyFebruary 13, 2023Parween F, Singh SP, Zhang HH, Kathuria N, Otaizo-Carrasquero FA, Shamsaddini A, Gardina PJ, Ganesan S, Kabat J, Lorenzi HA, et al.washing with Vascular Cell Basal Medium, cells were incubated with Alexa Fluor 594 streptavidin (1:500, 405240; BioLegend) for an additional 30 min. Cells were washed and fixed with 4% paraformaldehyde and after

(9) High-affinity, neutralizing antibodies to SARS-CoV-2 can be made without T follicular helper cellsScience immunologyFebruary 4, 2022Chen JS, Chow RD, Song E, Mao T, Israelow B, Kamath K, Bozekowski J, Haynes WA, Filler RB, Menasche BL, et al.BioLegend, 144606) overnight at 4°C. Secondary staining was performed with AF594 streptavidin (BioLegend, 405240) for 1 hr at room temperature. Images were acquired on a Nikon TiE Spinning Disk Confocal Microscope

(10) Toll-Like Receptor 4 Regulates Rabies Virus-Induced Humoral Immunity through Recruitment of Conventional Type 2 Dendritic Cells to Lymph OrgansJournal of virologyNovember 23, 2021Chen C, Zhang C, Li H, Wang Z, Yuan Y, Zhou M, Fu ZF, Zhao L.

(11) Improvement of HSV-1 based amplicon vectors for a safe and long-lasting gene therapy in non-replicating cellsMolecular therapy. Methods & clinical developmentJune 11, 2021Soukupová M, Zucchini S, Trempat P, Ingusci S, Perrier-Biollay C, Barbieri M, Cattaneo S, Bettegazzi B, Falzoni S, Berthommé H, et al.biotin anti-mouse/human CD45R/B220 antibody (1:100; 103204, BioLegend) associated with Alexa Fluor 594 Streptavidin (1:250; 405240, BioLegend). All secondary antibodies were incubated at room temperature for 2.5 h. Finally, sections

(12) Multiplexed CRISPR/CAS9-mediated engineering of pre-clinical mouse models bearing native human B cell receptorsThe EMBO journalJanuary 15, 2021Wang X, Ray R, Kratochvil S, Melzi E, Lin YC, Giguere S, Xu L, Warner J, Cheon D, Liguori A, et al.405235 Alexa Fluor 647 Streptavidin,BioLegend,Cat#: 405237 Alexa Fluor 594 Streptavidin,BioLegend,Cat#: 405240 "Pan B‐Cell Isolation Kit II, mouse",Miltenyi Biotec,Cat#: 130‐104‐443 SuperScript™ III Reverse Transcriptase,Thermo Fisher

(13) MEF2C regulates osteoclastogenesis and pathologic bone resorption via c-FOSBone researchJanuary 11, 2021Fujii T, Murata K, Mun SH, Bae S, Lee YJ, Pannellini T, Kang K, Oliver D, Park-Min KH, Ivashkiv LB.goat anti-rabbit IgG antibody (Vector Laboratories #BA-1000), Alexa Fluor 594 Streptavidin (Biolegend #405240), and Fluoroshield Mouting Medium (Abcam #ab104139) were used. Human osteoclast differentiation “Peripheral blood mononuclear cells

(14) A Peptidyl Inhibitor that Blocks Calcineurin-NFAT Interaction and Prevents Acute Lung InjuryJournal of medicinal chemistryNovember 12, 2020Dougherty PG, Karpurapu M, Koley A, Lukowski JK, Qian Z, Srinivas Nirujogi T, Rusu L, Chung S, Hummon AB, Li HW, et al.suppliers: α-mouse CD45-PerCP/Cy5.5 and streptavidin-Alexa Fluor 594 from Biolegend (Cat. No. NC0217834 and 405240), α-rabbit IgG-Alexa Fluor 488 from Cell Signaling (Cat. No. 4412S), and Human/Mouse/Rat NFATc3 from

(15) RAB11FIP5-Deficient Mice Exhibit Cytokine-Related Transcriptomic SignaturesImmunoHorizonsNovember 10, 2020Li D, Bradley T, Cain DW, Pedroza-Pacheco I, Aggelakopoulou M, Parks R, Barr M, Xia SM, Scearce R, Bowman C, et al.anti–IL-5 (clone no. TRFK4, catalog no. 504402, 1:100 dilution; BD), and streptavidin-AF594 (catalog no. 405240, 1:3000 dilution; BioLegend) Abs in permeabilization buffer (catalog no. 88-8824; Invitrogen). LIVE/DEAD

(16) A proximity-labeling proteomic approach to investigate invadopodia molecular landscape in breast cancer cellsScientific reportsApril 22, 2020Thuault S, Mamelonet C, Salameh J, Ostacolo K, Chanez B, Salaün D, Baudelet E, Audebert S, Camoin L, Badache A.were from Sigma Life Science. Biotinylated proteins were detected either by Alexa Fluor594 Streptavidin (405240, BioLegend) or Streptavidin-HRP (21130, Thermo Scientific). Proximity biotinylation. Cells stably expressing

(17) Expanding the Dynamic Range of Fluorescence Assays through Single-Molecule Counting and Intensity CalibrationJournal of the American Chemical SocietyOctober 24, 2018Lucas Smith, Manish Kohli, Andrew M. Smith(azide-PEG5000-NHS, 5000 Da) were purchased from Nanocs, Inc. Avidin-labeled phycoerythrin (405203) and Alexa 594 (405240) were purchased from BioLegend. Avidin-labeled Quantum Dot 605 nm (Q10103MP) and FluoSpheres (F8770)

(18) The Chemokine Receptor CCR8 Promotes the Migration of Dendritic Cells into the Lymph Node Parenchyma to Initiate the Allergic Immune ResponseImmunitySeptember 18, 2018Sokol CL, Camire RB, Jones MC, Luster AD.Reagent/Material,Source,Identifier Antibodies:,, Alexa Fluora 594 Streptavidin,Biolegend,Cat #: 405240 Alexa Fluroa 647 Streptavidin,Biolegend,Cat #: 405237 "Anti-Mouse CD8α, Clone: 53-6.7",Biolegend,Cat

(19) IFNAR1-Signalling Obstructs ICOS-mediated Humoral Immunity during Non-lethal Blood-Stage Plasmodium InfectionPLoS pathogensNovember 1, 2016Sebina I, James KR, Soon MS, Fogg LG, Best SE, Labastida Rivera F, Montes de Oca M, Amante FH, Thomas BS, Beattie L, et al.

(20) Engineered Lactobacillus rhamnosus GG expressing IgG-binding domains of protein G: Capture of hyperimmune bovine colostrum antibodies and protection against diarrhea in a mouse pup rotavirus infection modelVaccineJanuary 16, 2014Günaydın G, Zhang R, Hammarström L, Marcotte H.

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(1)

11/19/2021Great Streptavidin Stain Matt ArvedsonStreptavidin conjugates are used to detect biotin-stained markers. We use it to detect CCR7 biotin-stained samples.

Alexa Fluor® 594 Streptavidin from BioLegend

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Alexa Fluor® 594 Streptavidin from BioLegend

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