Fig 1: Role of JAK/STAT and TLR/JNK activation in RBP4-mediated HIV reactivation. a Reactivation of HIV-1 eGFP reporter proviruses in NT control (gray) or RELA, RELB, NFKB1, NFKB2 or NIK (green) KO J-Lat 10.6 cells treated with the indicated concentrations of holo-RBP4, TNFα or left untreated. Data are represented as mean ± SEM of three independent experiments with the NT sgRNA (gray) or two different targeting sgRNAs (green). p values are shown as measured by two-tailed Student’s t test with Welch’s correction. J-Lat 10.6 cells were pretreated with the indicated concentrations of STAT5 (b) or JNK (c) inhibitor or their combination (d). Four hours later, the cells were left untreated or treated with TNFα or the indicated concentrations of holo-RBP4. The percentage of cells showing reactivation of HIV-1 eGFP reporter proviruses (upper panel) and the cell viability (lower panel) were determined by flow cytometry. Data are represented as mean ± SEM of three independent experiments. p value are shown as measured by one-way ANOVA