Fig 1: miR-490-3p and PCBP1 protein expression inversely correlate in patients with breast cancer. (A) Spearman's rank correlation test demonstrated the inverse correlation between PCBP1 protein expression and' miR-490-3p expression in 10 patients with DCIS (red dots) and 10 patients with IDC (black dots) (P<0.05, Spearman's correlation coefficient-0.801). (B) Representative immunohistochemistry showing PCBP1 expression in breast cancer tissues from DCIS and IDC cases (2 each). DCIS, ductal carcinoma in situ; IDC, invasive ductal carcinoma.
Fig 2: PCBP1 is a target of miR-490-3p in breast cancer cells. (A) Complementary 7mer-m8 seed match between miR-490-3p and the 3′UTR of PCBP1 as predicted by TargetScan software. (B) Relative luciferase activity of transiently transfected luciferase reporter constructs containing either full-length or mutated (miR-490-3p binding site deleted) PCBP1 3′UTR in indicated cells. *P<0.05. Error bars represent the mean ± SD. (C) Relative luciferase activity of transiently transfected luciferase reporter constructs containing full-length PCBP1 3′UTR in indicated cells, alone or in combination with miR-490-3p mimic and antagomir. *P<0.05 compared to respective mock control. Error bars represent the mean ± SD. (D) Western blot analysis of PCBP1 in MDA-MB-468 and MDA-MB-231 cell lines transfected with miR-490-3p antagomir and mimic, respectively. TBP was used as a loading control.
Supplier Page from Abcam for Recombinant Human PCBP1 protein