Fig 1: ER-stress-induced PERK activity is higher in PERK-B/B-expressing MDMs than in PERK-A/A-expressing MDMs in vitro. A. Monocyte-derived macrophages isolated from PERK-A/A and PERK-B/B mice (n = 5–6/group) were treated with thapsigargin (Tg) or vehicle control (VC), and the protein levels of p-eIF2a, total eIF2a, PERK, and actin were determined with immunoblotting. Representative immunoblots are shown. B. Quantification of the immunoblots is shown. * p < 0.05, two-way repeated measures ANOVA with Sidak’s test for multiple comparisons.