Recombinant Human TNF-alpha Protein, CF 210-TA-020/CF from R&D Systems, a Bio-Techne Brand

Product Specs
ItemRecombinant Human TNF-alpha Protein, CF
CompanyR&D Systems, a Bio-Techne Brand
Price $272.00Supplier Page View Company Product Page
Catalog Number210-TA-020/CF
ApplicationsBioactivity
Conjugate/TagUnconjugated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution
Purity>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
NCBI Gene AliasesTNFSF2, DIF, TNF-alpha, TNLG1F, TNFA
NCBI Full Gene Nametumor necrosis factor
Molecular WeightObserved: 17 kDa, reducing conditions
Theoretical: 17 kDa
FormatLyophilized from a 0.2 µm filtered solution in PBS. *Small pack size (5 & 20 µg) is supplied as a 0.2 µm filtered solution in PBS
Source/Expression SystemE. coli-derived Recombinant Human TNF-alpha Protein, CF
Molecule NameTNF-alpha
SpeciesHuman
Size20 ug (also 5 ug, 100 ug, 2 mg)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Plasma Extracellular Vesicle Modulate Immune Cell Transcriptional Responses Following Acute Myocardial InfarctioniScienceMarch 20, 2026Lewis Timms, Raphaella Ridley, Adam Braithwaite, Charlotte Hooper, Tanvir Rafe, Carolin Lewe, Sebastian Lemke, Divya Ganesh, Zeyu Chen, Tafadzwa Kufazvinei, et al.recombinant proteins" ,, ,Life Technologies,434315 Recombinant Human TNF-alpha Protein,Biotechne,210-TA-020/CF LPS,Sigma-Aldrich,L2880-10 MG Recombinant Human IFN-gamma Protein,Biotechne,285-IF-100/CF ,, Critical

(2) Evaluation of risk promoting effects for age-related macular degeneration by estradiolPLoS OneMarch 9, 2026Inga-Marie Pompös, Dietrich Polenz, Norbert Kociok, Silvia Winkler, Olaf StraußUSA",CRL-2302 17ß-Estradiol,"Sigma-Aldrich, Darmstadt, Germany",50-28-2 TNFa,"R&D Systems, Minneapolis, USA",210-TA-020/CF RNeasy Plus Mini Kit,"Qiagen, Hilden, Germany",74136 QuantiTect Reverse Transcription Kit,"Qiagen,

(3) Molecular cargo of small EVs from NSCLC patient BALF and plasma: unveiling their role in airway inflammation and immune regulation in a novel human 3D bronchial modelCell Commun SignalOctober 15, 2025Magdalena Dlugolecka, Jacek Szymanski, Lukasz Zareba, Karolina Soroczynska, Zuzanna Homoncik, Malgorzata Polubiec-Kownacka, Ewa Frankiewicz, Diana Wierzbicka, Kannathasan Thetchinamoorthy, Joanna Domagala-Kulawik, et al.negative control, where we added PBS instead of EVs, and a positive control with TNF-α (1.75 µg/mL, cat. 210-TA-020CF, R&D Systems, BIOTECHNE). After 48 h of coculture (37 °C, 5% CO2, 100% humidity), the apical side was

(4) Commensal to pathogen switch in Streptococcus pneumoniae is influenced by a thermosensing master regulatorPLOS PathogensSeptember 30, 2025Shruti Apte, Greicy K. Bonifacio-Pereira, Sourav Ghosh, Srijit Kumar Mandal, Leena Badgujar, Krithika Gosavi, Elizabeth Pohler, Thomas E. Barton, Sian Pottenger, Alice Blake, et al.A21202), Anti-goat Alexa Fluor 633 (Invitrogen A21082). Recombinant Human TNF-α Protein (R&D systems 210-TA-020/CF) was used for induction of PAFR expression in A549 cells. In vitro transcription/translation To in vitro

(5) IDO‐1 Promotes Pulmonary Vascular Remodeling Via Kynurenine Pathway in Pulmonary Arterial HypertensionJournal of the American Heart AssociationAugust 19, 2025Zongye Cai, Ly Tu, Siyu Tian, Lin Deng, Yahong Fu, Carole Phan, Thierry P. P. van den Bosch, Karin Tran‐Lundmark, Bence M. Nagy, Horst Olschewski, et al.medium was 20 ng/mL for IL‐6 (interleukin‐6)/IL‐6Rα (8954‐SR/CF), TNF‐α (tumor necrosis factor alpha; 210‐TA‐020/CF), IFN‐γ (interferon‐gamma; 285‐IF‐100/CF), and TGF‐β1 (transforming growth factor beta 1; 7754‐BH‐005/CF).

(6) Therapeutic potential of AAV2-shmTOR gene therapy in reducing retinal inflammation and preserving endothelial Integrity in age-related macular degenerationScientific reportsMarch 19, 2025Kim J, Moon SY, Kang HG, Kim HJ, Choi JS, Lee SHS, Park K, Won SY.retinal degeneration. Materials and methods Chemicals and reagents Recombinant Human TNF-alpha protein (210-TA-020/CF) was purchased from R&D Systems (Minneapolis, MN). The following primary antibodies were used in this

(7) The isolation of VCAM-1+ endothelial cell-derived extracellular vesicles using microfluidicsExtracellular Vesicles and Circulating Nucleic AcidsFebruary 8, 2024Naveed Akbar, Evelyn Grace Luciani, Raheel Ahmad, Dasol Lee, Sara Veiga, Daniel Christopher Rabe, Shannon Leigh Stottstimulation HUVECs were treated with recombinant human tumor necrosis factor-α (TNF-α) (Biotechne, 210-TA-020/CF) at a concentration of 10 ng/mL for the described time points. Lentiviral transductions EA.hy926 cells

(8) Influence of Race and High Laminar Shear Stress on TNFR1 Signaling in Endothelial CellsInternational Journal of Molecular SciencesSeptember 29, 2023Maitha Aldokhayyil, Dulce H. Gomez, Marc D. Cook, Andreas N. Kavazis, Michael D. Roberts, Thangiah Geetha, Michael D. Brown2 h before stimulation or application of shear stress. A concentration of 30 ng/mL of TNF (cat. No. 210-TA-020/CF) from R&D Systems (Minneapolis, MN, USA) was used and incubated for 6 h. This dose was chosen based

(9) Inhibition of CDC20 potentiates anti-tumor immunity through facilitating GSDME-mediated pyroptosis in prostate cancerExperimental hematology & oncologyAugust 1, 2023Wu F, Wang M, Zhong T, Xiao C, Chen X, Huang Y, Wu M, Yu J, Chen D.was purchased from MedChemExpress (Cat. No. HY-12320). Recombinant Human TNF-alpha protein (rh-TNF-a, 210-TA-020/CF) was purchased from R and D Systems and reconstituted with sterilized PBS (pH 7.4) containing 0.1% BSA. Antibodies

(10) LOXL4, but not LOXL2, is the critical determinant of pathological collagen cross-linking and fibrosis in the lungScience advancesMay 26, 2023Ma HY, Li Q, Wong WR, N'Diaye EN, Caplazi P, Bender H, Huang Z, Arlantico A, Jeet S, Wong A, et al.

(11) Interferon-gamma is quintessential for NOS2 and COX2 expression in ER(-) breast tumors that lead to poor outcomeCell death & diseaseMay 11, 2023Cheng RYS, Ridnour LA, Wink AL, Gonzalez AL, Femino EL, Rittscher H, Somasundaram V, Heinz WF, Coutinho L, Rangel MC, et al.

(12) SARS-CoV-2 ORF3a positively regulates NF-κB activity by enhancing IKKβ-NEMO interactionVirus researchApril 15, 2023Nie Y, Mou L, Long Q, Deng D, Hu R, Cheng J, Wu J.11,668,019), dual-specific luciferase assay kits (Promega, E1980), human recombinant TNFα (R&D Systems, 210-TA-020/CF), BAY 11–7082 (MCE, HY-13,453), mouse anti-Flag (Sigma, F3165), rabbit anti-Flag (Proteintech, 20,543–1-AP),

(13) Structural homology screens reveal poxvirus-encoded proteins impacting inflammasome-mediated defensesbioRxiv : the preprint server for biologyApril 3, 2023Boys IN, Johnson AG, Quinlan M, Kranzusch PJ, Elde NC.

(14) Anti-inflammatory properties of antiangiogenic fucoidan in retinal pigment epithelium cellsHeliyonApril 1, 2023Dörschmann P, Seeba C, Thalenhorst T, Roider J, Klettner A.

(15) Fructose-1,6-bisphosphatase 1 dephosphorylates IκBα and suppresses colorectal tumorigenesisCell researchMarch 1, 2023Zhu W, Chu H, Zhang Y, Luo T, Yu H, Zhu H, Liu Y, Gao H, Zhao Y, Li Q, et al.

(16) Activation of Endothelial Large Conductance Potassium Channels Protects against TNF-α-Induced InflammationInternational journal of molecular sciencesFebruary 17, 2023Zyrianova T, Zou K, Lopez B, Liao A, Gu C, Olcese R, Schwingshackl A.

(17) Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cellsbioRxiv : the preprint server for biologyFebruary 13, 2023Parween F, Singh SP, Zhang HH, Kathuria N, Otaizo-Carrasquero FA, Shamsaddini A, Gardina PJ, Ganesan S, Kabat J, Lorenzi HA, et al.

(18) ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulationExperimental & molecular medicineFebruary 1, 2023Cho DI, Ahn MJ, Cho HH, Cho M, Jun JH, Kang BG, Lim SY, Yoo SJ, Kim MR, Kim HS, et al.

(19) Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisisNatureFebruary 1, 2023Nassour J, Aguiar LG, Correia A, Schmidt TT, Mainz L, Przetocka S, Haggblom C, Tadepalle N, Williams A, Shokhirev MN, et al.

(20) TGF-Beta Induces Activin A Production in Dermal Fibroblasts Derived from Patients with Fibrodysplasia Ossificans ProgressivaInternational journal of molecular sciencesJanuary 24, 2023de Ruiter RD, Wisse LE, Schoenmaker T, Yaqub M, Sánchez-Duffhues G, Eekhoff EMW, Micha D.

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Recombinant Human TNF-alpha Protein, CF

TNF-alpha Protein

TNF-alpha Protein

TNF-alpha Protein

TNF-alpha Protein

Recombinant Human TNF-alpha Protein, CF from R&D Systems, a Bio-Techne Brand

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R&D Systems, a Bio-Techne Brand

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Recombinant Human TNF-alpha Protein, CF from R&D Systems, a Bio-Techne Brand

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