Taq polymerase stop assays were used to assess the stabilization imparted by NCL to wild-type (wt) and mutant M4+5 LTRII+III+IV (M4+5) sequences. Taq polymerization was performed in the presence/absence of K+ and NCL (OriGene TP319082). Amplification of the wt template was performed at 37 C and 47 C; elongation was obtained at 37 C on the mutant template. The * symbol highlights stop regions. Figure cited from Nucleic Acids Res, PMID: 26354862

Dimethylsulfate protection analysis of the complex between NCL (OriGene TP319082) and LTR sequence oligonucleotides. A densitogram shown on the right quantifies band cleavage intensity: red and blue lines correspond to the LTR and NCL/LTR complex, respectively. G-tracts and their numbering are indicated on the left. The two gel portions derive from a single gel run. Figure cited from Nucleic Acids Res, PMID: 26354862

FRET melting analysis of the stabilization of NCL (OriGene TP319082) on the different length G4 LTR sequences.The results showed that NCL conferred the highest stabilization in the series to the LTR-II+III+IV construct, followed by LTR-III+IV. Progressively lower stabilization was observed for LTR-III and LTR-II, whereas LTR-IV was the least affected in the series. The negative control bovine serum albumin (BSA) did not afford any detectable stabilization to the selected sequences. Figure cited from Nucleic Acids Res, PMID: 26354862

SPR binding analysis of wild-type (wt) LTR-II+III+IV to immobilized NCL (OriGene TP319082). Oligonucleotide concentration range was 31.25 nM - 2000 nM. Sensograms are shown as gray lines and their respective fits as black lines. Figure cited from Nucleic Acids Res, PMID: 26354862