RecA M0249S from New England Biolabs

Product Specs
ItemRecA
CompanyNew England Biolabs
Price $78.00 Inquire
Catalog NumberM0249S
Size200 µg
ApplicationsDNA Manipulation
TypeProteins
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Cryo-EM architecture of a near-native stretch-sensitive membrane microdomainNatureAugust 15, 2024Jennifer M. Kefauver, Markku Hakala, Luoming Zou, Josephine Alba, Javier Espadas, Maria G. Tettamanti, Jelena Gajić, Caroline Gabus, Pablo Campomanes, Leandro F. Estrozi, et al.Mastermix (M0492, New England Biolabs), gel-purified DNA fragments were assembled using RecA recombinase (M0249, New England Biolabs). A plasmid containing mCherry was a gift from A. Michelot. The mCherry was cloned

(2) Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursorsNucleic Acids ResearchJuly 8, 2024Raquel Carreira, Tomas Lama-Diaz, Maria Crugeiras, F Javier Aguado, Marek Sebesta, Lumir Krejci, Miguel G Blancowere purified according to described procedures (55). RecA was obtained from New England Biolabs (#M0249S) and SSB from Thermo Fisher Scientific (#70032Z500G). Protein concentrations were determined using the

(3) An improved nucleic acid sequence-based amplification method mediated by T4 gene 32 proteinPLOS ONEMarch 24, 2022Yi Heng Nai, Egan H. Doeven, Rosanne M. GuijtNASBA, SSBs were added to the amplification mix in combination with the LEM cocktail at 2 μg of RecA (M0249, New England Biolabs (NEB)), 80 ng ET SSB (M2401S, NEB) or gp32 (M0300, NEB) at a concentration range

(4) HELQ is a dual-function DSB repair enzyme modulated by RPA and RAD51NatureJanuary 1, 2022Anand R, Buechelmaier E, Belan O, Newton M, Vancevska A, Kaczmarczyk A, Takaki T, Rueda DS, Powell SN, Boulton SJ.using same procedure. RPA–mRFP1 and RPA–eGFP were purified as described previously31. Recombinant RecA (M0249) and ET SSB (M2401) were commercially purchased from NEB, England. To purify GST and GST–BRC4, the BRC4

(5) Inhibition of SOS Response by Nitric Oxide Donors in Escherichia coli Blocks Toxin Production and HypermutationFrontiers in Cellular and Infection MicrobiologyDecember 22, 2021John K. Crane, Sarah R. Burke, Cassandra L. Alvarado2018) except that the purified RecA protein was from New England Biolabs instead of Abcam (NEB cat. no. M0249). The 10 X reaction buffer supplied with the RecA was used. As previously described, the ssDNA probe

(6) Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instabilityScientific ReportsDecember 1, 2020James Alexander Booth, Mário Špírek, Tekle Airgecho Lobie, Kirsten Skarstad, Lumir Krejci, Magnar Bjøråsof a previously published protocol22. RecA was diluted from concentrated stock (New England Biolabs M0249L) into Storage Buffer (50 mM phosphate/citrate buffer at pH 7, 50 mM NaCl), which was also used in no

(7) Modular microfluidics enables kinetic insight from time-resolved cryo-EMNature CommunicationsDecember 1, 2020Märt-Erik Mäeots et al.sodium-acetate, 1 mM DTT. Final reaction mixture also contained 1 mM ATP-γ-S, 10.57 μM RecA (NEB - M0249L) (0.4 mg/ml), 31.71 μM ssDNA (3-fold of RecA). The ssDNA used was 144-mer (TTTTTTCTCACACTCATTTTTTTTCTCACACTCATTTTTTTTCTCACACTCATTTTTTTTCTCACACTCATTTTTCCTATATTTATTCCTTTTCCTATATTTATTCCTTTTCCTATATTTATTCCTTTTCCTATATTTATTCCT).

(8) Influence of the C-Terminal Tail of RecA Proteins from Alkaline pH-Resistant Bacterium Deinococcus FicusACS OmegaAugust 11, 2020Hsiu-Fang Fan, Shu Su, Ying-An Kuo, Cyuan-Ji Chentail. Materials and Methods Proteins E. coli RecA was purchased from New England Biolabs (NEB, M0249S, native E. coli RecA without histidine tag accession ID: WP_000963143.1) and used without further purification.

(9) Separating Functions of the Phage-Encoded Quorum-Sensing-Activated Antirepressor QtipCell Host & MicrobeApril 1, 2020Justin E. Silpe, Andrew A. Bridges, Xiuliang Huang, Daniela R. Coronado, Olivia P. Duddy, Bonnie L. BasslerS9102S HALO-TMR,Promega,Cat# G8251 HALO-Alexa660,Promega,Cat# G8471 RecA and RecA Buffer,NEB,Cat# M0249L ATP-γ-S,Sigma,Cat# A1388–5MG Novex 6% DNA Retardation Gel,Thermo,Cat# EC63655BOX SYBR Green I Nucleic

(10) Mycobacterial Mutagenesis and Drug Resistance Are Controlled by Phosphorylation- and Cardiolipin-Mediated Inhibition of the RecA CoproteaseMolecular cellOctober 4, 2018Wipperman MF, Heaton BE, Nautiyal A, Adefisayo O, Evans H, Gupta R, van Ditmarsch D, Soni R, Hendrickson R, Johnson J, et al.MC2155,PMID:2082148,N/A Mycobacterium tuberculosis Erdman,N/A,N/A Biological Samples,, E. coli RecA,New England Biolabs,M0249L "Trypsin-ultra, Mass Spectrometry Grade",NEB,P8101S Proteinase K,Fisher,FEREO0491 "Chemicals, Peptides,

(11) Human RAD51 rapidly forms intrinsically dynamic nucleoprotein filaments modulated by nucleotide binding stateNucleic Acids ResearchMay 4, 2018Mário Špírek, Jarmila Mlčoušková, Ondrej Beláň, Máté Gyimesi, Gábor M Harami, Eszter Molnár, Jiri Novacek, Mihály Kovács, Lumir KrejciRecA protein accordingly to (11), alternatively was purchased from New England Biolabs (catalog number M0249). Stopped-flow experiments and data analysis Stopped-flow mixes were performed in SF50 buffer (50

(12) Stable Nuclei of Nucleoprotein Filament and High ssDNA Binding Affinity Contribute to Enhanced RecA E38K Recombinase ActivityScientific ReportsDecember 1, 2017Chih-Hao Lu, Ting-Tzu Chang, Chia-Chuan Cho, Hui-Cin Lin, Hung-Wen LiA-3′). Protein and buffer condition E. coli wild-type RecA was purchased from New England Biolabs (NEB, M0249) without further purification. RecA E38K mutant was purified as previously described11,41, and a brief

(13) RecA stimulates AlkB-mediated direct repair of DNA adductsNucleic Acids ResearchOctober 14, 2016Gururaj Shivange, Mohan Monisha, Richa Nigam, Naveena Kodipelli, Roy Anindya7.0 or 20 mM Tris–HCl, pH 8.0 or 9.0. E. coli RecA (EcRecA) was purchased from New England Biolabs (M0249L). For the analysis of EcRecA, 0.5 mg (35 μM) of AlkB was mixed with 0.735 mg (35 μM) of RecA in 0.5

(14) Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathwayseLifeAugust 25, 2015Sukrit Suksombat, Rustem Khafizov, Alexander G Kozlov, Timothy M Lohman, Yann R Chemlatetramer) as described previously (Roy et al., 2009). E. coli RecA was purchased from New England Biolabs (M0249S; Ipswich, MA). Single-stranded DNA construct The single-stranded DNA construct consisted of three

(15) Insights into the environmental reservoir of pathogenic Vibrio parahaemolyticus using comparative genomicsFrontiers in MicrobiologyMarch 24, 2015Tracy H. Hazen, Patricia C. Lafon, Nancy M. Garrett, Tiffany M. Lowe, Daniel J. Silberger, Lori A. Rowe, Michael Frace, Michele B. Parsons, Cheryl A. Bopp, David A. Rasko, Patricia A. Sobecky

(16) Quality Control of Purified Proteins Involved in Homologous RecombinationMethods in Molecular BiologyJune 4, 2014Xiao-Ping Zhang, Wolf-Dietrich HeyerCommercial Form I ΦX174 (NEB, N3021L) and virion DNA (circular ssDNA, NEB, N3023L). RecA protein (NEB, M0249S) as positive control. 30 °C water bath. 4 °C bench top centrifuge. Liquid scintillation vials

(17) Opposing Roles for Two Molecular Forms of Replication Protein A in Rad51-Rad54-Mediated DNA Recombination in Plasmodium falciparummBioJuly 1, 2013Anusha M. Gopalakrishnan, Nirbhay KumarµM ΦX virion dsDNA (NEB), 1 mM DTT, and 2 µM concentrations of the proteins (RecA obtained from NEB M0249S, PfRad51, or PfRad54) to be tested for functional activity. The reaction mixture (135 µl) was preincubated

(18) Force and ATP hydrolysis dependent regulation of RecA nucleoprotein filament by single-stranded DNA binding proteinNucleic Acids ResearchJanuary 1, 2013Hongxia Fu, Shimin Le, Hu Chen, K. Muniyappa, Jie Yanis M3011). E. coli RecA protein was purchased from New England BioLabs, USA (The catalog number is M0249L) or purified as previously described (25). The 576 bp DNA construct was generated by PCR using DreamTaq

(19) A study on the mechanism of RecA in homologous recognition by using single molecule fluorescence trackingActa Physica SinicaJanuary 1, 2012Pang Zhe, Wang Shuang, Li Hui, Xu Chun-Hua, Li Ming, Beijing National Laboratory for Condensed Matter Physics and CAS Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China溶液中. 218701-2 物理学报 Acta Phys. Sin. Vol. 61, No. 21 (2012) 218701 实验中使用的 RecA 是直接购买的蛋白 (NEB- 双链 DNA 的固定是通过液流拉伸的方法实 M0249L), 分子量为 38.5 kD, 浓度为 2 mg/mL. 实验现的. 使用 RecA 缓冲液将双链 DNA 配成浓度时使用 RecA 缓冲液进行稀释, RecA 缓冲液配方为 5

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RecA

RecA from New England Biolabs

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RecA from New England Biolabs

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