ProtoScript II Reverse Transcriptase M0368L from New England Biolabs

Product Specs
ItemProtoScript II Reverse Transcriptase
CompanyNew England Biolabs
Price $198.00 Inquire
Catalog NumberM0368L
Size10000 units
ApplicationsDNA Analysis
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(141) Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA EditingCell ReportsNovember 3, 2020Ryan J. Marina, Kristopher W. Brannan, Kevin D. Dong, Brian A. Yee, Gene W. YeoNEBNext Ultra II Q5 Master Mix,New England Biolabs,Cat# M0544S Protoscript II Reverse Transcriptase,New England Biolabs,Cat# M0368L Agencourt AMPure XP beads,Beckman Coulter,Cat# A63881 ,, Critical Commercial Assays,Critical Commercial

(142) Integrator-Dependent and Allosteric/Intrinsic Mechanisms Ensure Efficient Termination of snRNA TranscriptionCell reportsOctober 27, 2020Davidson L, Francis L, Eaton JD, West S.,Fisher Scientific,13778075 Asunaprevir,, Auxin,Sigma,I2886 Hygromycin,Life Technologies,10687010 Neomycin,Sigma,345810 LUNA qPCR mastermix,NEB,M3003 Protoscript II,NEB,M0368 TURBO DNase,Fisher Scientific,AM2238 Benzonase,Sigma,E1014 Tri-reagent,Fisher Scientific,AM9738 Sheep

(143) Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunitiesBiosensors and BioelectronicsOctober 15, 2020Jeanne E. van Dongen, Johanna T.W. Berendsen, Renske D.M. Steenbergen, Rob M.F. Wolthuis, Jan C.T. Eijkel, Loes I. Segerink

(144) Modulation of Tmem135 Leads to Retinal Pigmented Epithelium Pathologies in MiceInvestigative ophthalmology & visual scienceOctober 1, 2020Landowski M, Grindel S, Shahi PK, Johnson A, Western D, Race A, Shi F, Benson J, Gao M, Santoirre E, et al.Oligo d(T)18 primer (#S1316S; NEB, Ipswich, MA, USA) and the ProtoScript II Reverse Transcriptase (#M0368L; NEB) following the manufacturer's protocol. Expression of each gene was assessed in triplicate reactions

(145) Ultra-sensitive and high-throughput CRISPR-p owered COVID-19 diagnosisBiosensors and BioelectronicsSeptember 15, 2020Zhen Huang et al.UK). EnGen® Lba Cas12a (#M0653T) and NEBuffer™ 2.1 (#B7202S), ProtoScript® II Reverse Transcriptase (#M0368S) were purchased from New England Biolabs (Ipswich, USA). Primers, gRNA, probes (Table S1), including those

(146) SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed CasesmSystemsAugust 25, 2020Fuqing Wu et al.reverse transcription with random hexamers (IDT) based on manufacturer’s protocol (NEB catalog no. M0368). PCR includes TaqMan Fast Advanced master mix (4444557; ThermoFisher Scientific); CDC N1, N2, and N3

(147) A low-bias and sensitive small RNA library preparation method using randomized splint ligationNucleic Acids ResearchAugust 20, 2020Sean Maguire, Gregory J S Lohman, Shengxi Guan20 units of Murine RNase inhibitor (M0314), 200 units of Protoscript II reverse transcriptase (NEB M0368) and nuclease free water to bring the final volume to 50 μl. This reaction was then incubated for 1

(148) Adipocyte Piezo1 mediates obesogenic adipogenesis through the FGF1/FGFR1 signaling pathway in miceNature communicationsMay 8, 2020Wang S, Cao S, Arhatte M, Li D, Shi Y, Kurz S, Hu J, Wang L, Shao J, Atzberger A, et al.DNA synthesis was performed using the ProtoScript II Reverse Transcription kit (New England BioLabs, M0368S). Primers were designed with the online tool provided by Roche and quantification was performed using

(149) Ultra-high throughput single-cell analysis of proteins and RNAs by split-pool synthesisCommunications biologyMay 7, 2020O'Huallachain M, Bava FA, Shen M, Dallett C, Paladugu S, Samusik N, Yu S, Hussein R, Hillman GR, Higgins S, et al.mM Aminoallyl dUTP (Thermo Scientific, R0091), 5000 units ProtoScript II Reverse Transcriptase (NEB, M0368), 1X ProtoScript II reaction buffer (NEB, B0368), 500 units of SUPERase-In RNase Inhibitor (Ambion,

(150) Inflammasome-mediated antagonism of type I interferon enhances Rickettsia pathogenesisNature microbiologyMay 1, 2020Burke TP, Engström P, Chavez RA, Fonbuena JA, Vance RE, Welch MD.was quantified using a NanoDrop ND-1000 and 200 ng RNA was in vitro transcribed (ProtoScript II, NEB, M0368S) and diluted 10x in sterile nuclease-free water. Real-time PCR was performed using SYBR Green (Thermo Fisher Scientific

(151) Machine learning of reverse transcription signatures of variegated polymerases allows mapping and discrimination of methylated purines in limited transcriptomesNucleic Acids ResearchApril 17, 2020Stephan Werner et al.No.,Supplier #1,M-MuLV,MMLV,M0253S,New England Biolabs #2,AMV,AMV,M0277S,New England Biolabs #3,ProtoScript® II,MMLV,M0368S,New England Biolabs #4,GoScript™,MMLV,A5003,Promega #5,SuperScript™ III,MMLV,18080044,Thermo Fisher Scientific

(152) Diapedesis-Induced Integrin Signaling via LFA-1 Facilitates Tissue Immunity by Inducing Intrinsic Complement C3 Expression in Immune CellsImmunityMarch 17, 2020Kolev M, West EE, Kunz N, Chauss D, Moseman EA, Rahman J, Freiwald T, Balmer ML, Lötscher J, Dimeloe S, et al.Reverse Transcription Kit,Applied Biosystems,Cat. #4368814 Protoscrip II,New England Biolabs,Cat. #M0368S RNAqueous-Micro Total RNA Isolation Kit,Thermo Fisher Scientific,Cat. #AM1931 Experimental Models:

(153) Translational efficiency across healthy and tumor tissues is proliferation‐relatedMolecular Systems BiologyMarch 9, 2020Xavier Hernandez‐Alias, Hannah Benisty, Martin H Schaefer, Luis SerranoT4 Polynucleotide Kinase,New England BioLabs,Cat#M0201 ProtoScript II Reverse Transcriptase,New England BioLabs,Cat#M0368 miRNeasy Mini kit,Qiagen,Cat#217004 15% TBE–urea gels,"NOBEX, Invitrogen",Cat#EC6885BOX RNeasy MinElute

(154) Programmable CRISPR interference for gene silencing using Cas13a in mosquitoesJournal of GenomicsMarch 1, 2020Aditi Kulkarni, Wanqin Yu, Alex S. Moon, Ashmita Pandey, Kathryn A. Hanley, Jiannong Xuto remove genomic DNA contamination, and then 1μg RNA was converted to cDNA using Protoscript II RT (M0368S, New England Biolabs) following the manufacturer's instruction. The PCR assays were performed using

(155) PERK-mediated expression of peptidylglycine α-amidating monooxygenase supports angiogenesis in glioblastomaOncogenesisFebruary 13, 2020Soni H, Bode J, Nguyen CDL, Puccio L, Neßling M, Piro RM, Bub J, Phillips E, Ahrends R, Eipper BA, et al.One microgram of RNA was used to synthesize cDNA using ProtoScript® II Reverse Transcriptase, NEB (#M0368L, New England Biolabs (NEB), Ipswich, USA). Real-time PCR was performed by mixing 10 ng of cDNA, 0.5

(156) Dicer- and Bulge Stem Cell-Dependent MicroRNAs During Induced Anagen Hair Follicle DevelopmentFrontiers in cell and developmental biologyJanuary 1, 2020Vishlaghi N, Lisse TS.using 200 ng total RNA with the ProtoScript® First Strand cDNA Synthesis kit (New England Biolabs; M0368) utilizing random hexamers. All cDNAs were amplified under the following conditions: 95°C for 10 min

(157) Har-P, a short P-element variant, weaponizes P-transposase to severely impair Drosophila developmenteLifeDecember 17, 2019Satyam P Srivastav, Reazur Rahman, Qicheng Ma, Jasmine Pierre, Saptaparni Bandyopadhyay, Nelson C LauE7805, commerical assay or kit,First Strand cDNA Synthesis Kit using ProtoScript II,New England Biolabs Inc,Cat No. M0368, commerical assay or kit,NEBNext Small RNA Library Prep Set for Illumina,New England Biolabs Inc,Cat

(158) Systematic evaluation and optimization of the experimental steps in RNA G-quadruplex structure sequencingScientific ReportsDecember 1, 2019Pui Yan Yeung, Jieyu Zhao, Eugene Yui-Ching Chow, Xi Mou, HuiQi Hong, Leilei Chen, Ting-Fung Chan, Chun Kit KwokTGIRT™-III Enzyme (100U final) (InGex), or 200U/μl ProtoScript® II Reverse Transcriptase (100U final) (NEB, M0368S). The guanosine (G) sequencing reaction was prepared by addition of 1 μl of 10 mM dideoxycytidine (ddC)

(159) Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation ApplicationsACS chemical biologyNovember 15, 2019Ward CC, Kleinman JI, Brittain SM, Lee PS, Chung CYS, Kim K, Petri Y, Thomas JR, Tallarico JA, McKenna JM, et al.(Thermo Fisher, 15596026) per the manufacturer’s specification. NEB Protoscript II Reverse Transcriptase (NEB, M0368) was used to synthesize cDNA with oligo dT primers per the manufacturer’s specification. Gene expression

(160) Myogenic vasoconstriction requires G12/G13 and LARG to maintain local and systemic vascular resistanceeLifeSeptember 24, 2019Ramesh Chennupati, Angela Wirth, Julie Favre, Rui Li, RÃ©my Bonnavion, Young-June Jin, Astrid Wietelmann, Frank Schweda, Nina Wettschureck, Daniel Henrion, et al.using Qiagen RNeasy mini kit, and cDNA was preamplified using ProtoScript II Reverse Transcriptase (M0368S, New England BioLabs). Primers were designed with the online tool provided by Roche, and quantification

Be the First to Write a Review!

ProtoScript II Reverse Transcriptase

ProtoScript II Reverse Transcriptase from New England Biolabs

New England Biolabs

Citations (188)

Reviews

ProtoScript II Reverse Transcriptase from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map