NEBNext High-Fidelity 2X PCR Master Mix M0541S from New England Biolabs

Product Specs
ItemNEBNext High-Fidelity 2X PCR Master Mix
CompanyNew England Biolabs
Price $92.00 Inquire
Catalog NumberM0541S
Size50 reactions
ApplicationsDNA Analysis, PCR
TypeMaster Mix
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Spatial epigenomics reveals stromal niche-associated regulatory programs across pancreatic neuroendocrine tumor gradesiScienceNovember 20, 2026Dejiang Wang, Xiangjun Di, Fu Gao, Gaocai Li, Liyun Lin, Shujie He, Di Zhang, Junyi Xu, Yu Jin, Yuanxin Liang, et al.,New England Biolabs T4 DNA Ligase Reaction Buffer,B0202S,New England Biolabs NEBNext High-Fidelity 2X PCR Master Mix,M0541L,New England Biolabs DPBS,14190144,Thermo Fisher Scientific DNA Clean & Concentrator-5,D4014,Zymo Research dNTP mix

(2) Inflammatory memory primes the intestine for enhanced regenerative potentialNat CommunOctober 27, 2026Borja Arozamena, Ilias Moraitis, Loris Mularoni, Olga Wienskowska, Jasin Taelman, Milica Stefanovic, Ignasi Modolell Farré, Ferran Guedea, Mònica Díaz, Jordi Guiuwere pre-amplified by 5-PCR cycles using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, M0541S) and the required number of PCR cycles were determined by qPCR. The final libraries were purified using

(3) Survey of nucleotide-specific Rab GTPase interactions reveals multiple Rab effectorsbioRxivOctober 23, 2026Tabitha A. Peterson, Christopher P. Ptak, Mark A Stamnes, Robert C. Piperpools (Transomic Technologies, Huntsville, AL) were amplified by PCR using NEBNext polymerase (cat#: M0541, New England Biolabs, Ipswich, MA) and a biotinylated primer recognizing the Gateway vector backbone

(4) Protocol for profiling chromatin accessibility in human small-cell lung cancer FFPE-derived samples using Omni-ATAC-seqSTAR ProtocOctober 23, 2026Eralda Salataj, Kenta Kawasaki, Joan Massagué, Charles M. Rudin, Iestyn Whitehouse, Richard Koche, Pierre-Jacques HamardCoulter,B23319 DAPI,FisherScientific,P36962 Propidium Iodide (PI),Denovix,CD-PI-1.5 1× PBS,Fisher Scientific,MT21040CV NEBNext 2× MasterMix,NEB,M0541L Qubit™ 1× dsDNA High Sensitivity (HS) and Broad Range (BR) Assay Kits,ThermoFisher Scientific,Q32854Q32853 ,, Critical

(5) XIST drives X-chromosome inactivation and safeguards female extraembryonic cells in humansNat CommunOctober 22, 2026Amitesh Panda, Léo Carrillo, Bradley Philip Balaton, Jeanne Brouillet, Solomon Nshemereirwe, Jarne Bonroy, Charbel Alfeghaly, Romina Facchinello, Sherif Khodeer, Nicolas Peredo, et al.used for tagmentation of the regions bound by the target of interest. NEBnext PCR master mix (NEB, M0541) was used for tagmented fragment amplification and library preparation. DNA libraries were prepared

(6) HDAC inhibitor treatment restores transcriptome, chromatin accessibility, and memory deficits in a mouse model for Down syndromeiScienceOctober 16, 2026Cesar Sierra, René A.J. Crans, Mara Dierssen,Applied Biosystems,Cat#N8080119 Digitonin,Promega,Cat#G9441 NEBNext High-Fidelity 2X PCR Master Mix,NEB,Cat#M0541S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, GenElute

(7) Optimized parameters for CRISPR-Cas9 interference library designCell GenomicsOctober 14, 2026Smriti Srikanth, Fengyi Zheng, Laura M. Drepanos, Spencer T. Shah, Eleanor G. Kaplan, Daytan Gibson, Grace O. Lynch, Allison T. Uebele, Ganna Reint, Sarra Merzouk, et al.assays,Critical commercial assays ,, NEBNext High Fidelity 2× PCR Master Mix,New England Biolabs,Cat#M0541 Mag-Bind Blood & Tissue DNA HDQ Kit,Omega Bio-Tek,Cat#M6399-00 Titanium Taq DNA Polymerase,Takara,Cat#639209 Agencourt

(8) High-throughput identification of chromatin-opening transcription factorsbioRxivOctober 9, 2026Aidan Keith, Shruti Jain, Riza M. Daza, Chengxiang Qiu, Pierre-Luc Germain, Jay Shendure, Silvia Domcke(Zymo Research, D4013), eluted in EB buffer (10 mM Tris), and amplified using 2x NEBNext HF PCR Mastermix (NEB, M0541L) and indexed Illumina primers using SYBR green to monitor amplification and preserve library complexity.

(9) Clonal lineage tracing and parallel multiomics profiling reveal transcriptional heterogeneity induced by ARID1A deficiencySci AdvOctober 9, 2026Kenichi Miyata, Liying Yang, Yuxiao Yang, Kohei Kumegawa, Reo Maruyamadouble-stranded barcode-shRNA oligonucleotides: 25 μl of NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541), 1 μl of 100 μM cell barcode, 2 μl of 100 μM reverse extension primer (5′-TGCAGCATGCGTCTCACAACG-3′),

(10) ATRX deficiency drives aberrant type I interferon signalling through cGAS-dependent transcriptional dysregulationNat CommunOctober 8, 2026Marie-Thérèse A. El-Daher, Eve L. White, Andreas Fellas, Graeme Grimes, Simone Pelliciari, Gaofeng Zhu, Tom A. Tait, Carolina Uggenti, Natalie Blair, Erisa Nita, et al.with 0.67% Triton-X100. Barcoded i5 (v2_Ad1) and i7 (v2_Ad2) primer78 and NEBNext 2x Master Mix (NEB, M0541L) were added, and tagmented fragments were amplified by PCR. PCR clean-up was performed using AMPure

(11) Live-cell transcriptomics with engineered virus-like particlesCellOctober 1, 2026Mohamad Ali Najia, Anna Le, Jacob Borrajo, Lauren Pruett, FuNien Tsai, Ryan Ogi, Jeremy Y. Huang, Linda G. Griffith, George Q. Daley, Paul C. BlaineyAMPure XP SPRI beads followed by 12 cycles of PCR with NEBNext High Fidelity polymerase (NEB cat. no. M0541L) to incorporate Illumina indices and flow cell handles. The libraries were assessed using a High Sensitivity

(12) A druggable chromatin vulnerability in suppressive neutrophils restores antitumor immunitybioRxivOctober 1, 2026Fan Yang, Yi Bao, Yuanyuan Qiao, Gabriel Cruz, Eleanor Young, Rahul Mannan, Yuping Zhang, Matt Goodrich, Yizhi Cao, Jie Luo, et al.purified by Qiagen minElute column and PCR-amplified with the Next High-Fidelity 2X PCR Master Mix (NEB, M0541L) following the original protocol. Optimal PCR cycles were determined via qPCR to avoid over-amplification.

(13) Integration of the D-Genome Reshapes Gene Transcription, Chromatin Architecture, and Metabolome of Allohexaploid Wheat, Leading to Enhanced Environmental AdaptabilityAdv Sci (Weinh)September 27, 2026Yanyan Liu, Tao Zhu, Ziru Zhou, Wei Chen, Chao He, Xinkai Zhou, Xin Wang, Chuanye Chen, Bo Yang, Jiaqi Wei, et al.(QIAGEN, 28006). ATAC‐seq libraries were prepared with NEBNext High‐Fidelity 2X PCR Master Mix (NEB, M0541L) and TruePrep Index Kit V2 for Illumina (Vazyme, TD202). Libraries were sequenced using Illumina NovaSeq 6000 system

(14) Rapid detection of cultivated truffle fungi ( Tuber ) from truffles and ectomycorrhizal root tips using loop-mediated isothermal amplificationApplied and Environmental MicrobiologySeptember 23, 2026Alassane Sow, Andrew Cooper, Inga Meadows, Gregory Bonito, Matthew E. Smithnot included in our estimate. c M1800L NEB WarmStart Colorimetric LAMP 2× Master Mix (DNA & RNA). d M0541L NEBNext High-Fidelity 2× PCR Master Mix. e M3003L NEB Luna Universal qPCR Master Mix. f Eurofins Genomics

(15) A temporal single cell atlas of dynamic chromatin accessibility during murine lung maturationNature CommunicationsSeptember 18, 2026Xin Wang, Yuhao Deng, Yanru Zhang, Yurong Chen, Hang Li, Min Zhang, Zhizhuo Cao, Shaojun Zhang, Fang Wangof sample from the step above was mixed with 50 μl of NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S), 2 μl of 25 μM Partial_P5 primer, 2 μl of 25 μM Read2 Primer (sequences provided in Supplementary Data

(16) Coenzyme A activates TLR4 and enhances alternative macrophage activation in line with MyD88 agonismEMBO ReportsSeptember 18, 2026Anthony E Jones, Amy Rios, Neira Ibrahimovic, Andréa B Ball, Carolina Chavez, Nicholas A Bayley, Natalie A Falta, Marisa Mekkittikul, Clarissa Carrillo, Wei Yuan Hsieh, et al.hb-det2 Tn5 transposase,Illumina,Cat# 20034197 NEBNext High-Fidelity PCR Mix,New England Biolabs,Cat# M0541S Software,Software,Software Agilent Seahorse XF Wave,Agilent,https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-software/seahorse-wave-pro-software-2007523 Agilent

(17) A retinoic acid-Rfx6 cascade modulates Wnt/Bmp-dependent gut tube patterning: insight into the molecular basis of Mitchell–Riley SyndromeDevelopmentSeptember 15, 2026Scott A. Rankin, Jacek Biesiada, Kyle W. McCracken, Riya K. Patel, Mehek Rana, David M. Luedeke, Daniel O. Kechele, James M. Wells, Aaron M. Zornwas amplified in a reaction with 25 µl NEBNext High-Fidelity 2× PCR Mastermix (New England Biolabs, M0541L) and custom 25 µm Nextera PCR Primers (Ad1_noMx universal primer, 0.5 µm Ad2.x indexing primer). PCR

(18) Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growthCellSeptember 15, 2026Kalil G. Abdullah, Kenji Miki, Charles K. Edgar, Shuangcheng Alivia Wu, Yi Xiao, Yuan-Tai Huang, Richard Drexler, Saritha Krishna, Vinesh T. Puliyappadamba, Li Wei Rachel Tay, et al.Clean and Concentrator-5 Kit,Zymo Research,Cat# D4013 NEBNext 2x MasterMix,New England Biolabs,Cat# M0541S RNeasy Plus Universal Kit,Qiagen,Cat# 73404 Neural Tissue Dissociation Kit (P),Miltenyi Biotec,Cat#

(19) Sea nettle jellyfish venom targets proteoglycans to cause cell death and painSci AdvSeptember 4, 2026Christopher E. Denes, Tian Y. D'Araujo, Felicity Chung, Man-Tat Lau, Adam Cole, Nicholas R. Casewell, Eivind A. B. Undheim, G. Gregory Neely5′-GAGCCAATTCCCACTCCTTTCAA-3′) was combined with 5 μg of gDNA and NEBNext High-Fidelity 2× PCR Master Mix (NEB, no. M0541) at a final 1× concentration, necessitating multiple 5-μg reactions to cover diversity controls. Thermocycling

(20) Mapping genetic modifiers of epimutation rates identifies VIM2/4 as dosage-sensitive negative regulators of CG methylation maintenanceNature PlantsSeptember 3, 2026Zhilin Zhang, Wilma Wanney, Yangyang Xu, Johan Zicola, Angela M. Hancock, Robert J. Schmitz, Frank JohannesClean-up, #T1030) and then amplified for 10–12 cycles using Q5 polymerase (NEBNext HiFi 2x PCR Mastermix, #M0541). PCR conditions were followed as described previously78. Amplified libraries were purified with AMPure

Be the First to Write a Review!

NEBNext High-Fidelity 2X PCR Master Mix

NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs

New England Biolabs

Citations (1005)

Reviews

NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map