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NEBNext High-Fidelity 2X PCR Master Mix
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NEBNext High-Fidelity 2X PCR Master Mix
NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
Be the first to write a review!
Citations:
(491)
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Supplier Page from
New England Biolabs for
NEBNext High-Fidelity 2X PCR Master Mix
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NEBNext High-Fidelity 2X PCR Master Mix
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
M0541S
Quantity
50 reactions
Applications
DNA Analysis, PCR
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Master Mix
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (491)
(1)
Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni
Wellcome Open Research
February 13, 2024
Chrystelle Lasica, Ronaldo de Carvalho Augusto, Hélène Moné, Gabriel Mouahid, Cristian Chaparro, Anne-Clémence Veillard, Agnieszka Zelisko-Schmidt, Christoph Grunau
in a thermocycler for five minutes at 55°C. Then, 25 µL of 2xPCR NEB master mix (
New England Biolabs
M0541L
, lot 10067165) was added to each input. The end-repair and de-cross-link were performed in a thermocycler
(2)
Dose rate dependent reduction in chromatin accessibility at transcriptional start sites long time after exposure to gamma radiation
Epigenetics
December 31, 2023
Hildegunn Dahl, Jarle Ballangby, Torstein Tengs, Marcin W. Wojewodzic, Dag M. Eide, Dag Anders Brede, Anne Graupner, Nur Duale, Ann-Karin Olsen
µL) was performed using 25 µL 2× NEBnext High-Fidelity PCR Master Mix (
New England BioLabs
(cat. nr:
M0541L
), Ipswich, MA, USA) and 2.5 µL forward and reverse Nextera DNA CD indexes (Illumina, San Diego, CA,
(3)
NET formation is a default epigenetic program controlled by PAD4 in apoptotic neutrophils
Sci Adv
December 22, 2023
Yanfang Peipei Zhu, Mary Speir, ZheHao Tan, Jamie Casey Lee, Cameron J. Nowell, Alyce A. Chen, Hajera Amatullah, Ari J. Salinger, Carolyn J. Huang, Gio Wu,
et al.
i7 primer and then amplified with the NEBNext HiFi 2x PCR Master mix (
New England Biolabs
Inc. (EB),
M0541
). The libraries were cleaned-up with solid-phase reversible
immobilization
(SPRI) paramagnetic beads
(4)
Sperm chromatin accessibility's involvement in the intergenerational effects of stress hormone receptor activation
Transl Psychiatry
December 8, 2023
Vincent Fischer, Miriam Kretschmer, Pierre-Luc Germain, Jasmine Kaur, Sergio Mompart-Barrenechea, Pawel Pelczar, David Schürmann, Primo Schär, Katharina Gapp
the NEBNext high-Fidelity 2X master mix (
New England Biolabs
, Ipswich, Massachusetts, United States,
M0541
) and 2.5 uM Nextera primers (Illumina, San Diego, California, United States, FC-141-1007) at 72 °C for
(5)
Dystrophin deficiency impairs cell junction formation during embryonic myogenesis
bioRxiv
December 7, 2023
Elise Mozin, Emmanuelle Massouridès, Virginie Mournetas, Clémence Lièvre, Audrey Bourdon, Dana L Jackson, Jonathan S Packer, Cole Trapnell, Caroline Le Guiner, Oumeya Adjali,
et al.
A63880
Ethanol,Decon Labs,Cat# 2716
NEBNext® High Fidelity 2X PCR Master Mix
,
New England Biolabs
,Cat#
M0541
"Novex® TBE-PAGE gels, 6%",
Thermo Fisher
,Cat#
EC6265BOX
Quick-Load 2-log DNA Ladder
,
New England Biolabs
,Cat#
(6)
Immunoproteasome function maintains oncogenic gene expression in KMT2A-complex driven leukemia
Mol Cancer
December 4, 2023
Nuria Tubío-Santamaría, Ashok Kumar Jayavelu, Tina M. Schnoeder, Theresa Eifert, Chen-Jen Hsu, Florian Perner, Qirui Zhang, Daniela V. Wenge, Fynn M. Hansen, Joanna M. Kirkpatrick,
et al.
barcoded i7 primer and amplified using
NEBNext High Fidelity 2x PCR Master Mix
(
New England Biolabs
,
M0541S
) in a thermocycler using the following conditions: 98°C for 30 seconds; 14 cycles of 98°C for 10 seconds,
(7)
Systematic characterization of the HOXA9 downstream targets in MLL-r leukemia by noncoding CRISPR screens
Nature Communications
November 28, 2023
Shaela Wright, Xujie Zhao, Wojciech Rosikiewicz, Shelby Mryncza, Judith Hyle, Wenjie Qi, Zhenling Liu, Siqi Yi, Yong Cheng, Beisi Xu,
et al.
100 × SYBR Green I (Invitrogen, S-7563), and 25 μL NEBNext® High-Fidelity 2 × PCR Master Mix (
NEB
,
M0541
).
PCR
products were subsequently re-amplified for another 6
PCR
cycles, followed by
purification
using
(8)
Noncanonical Activity of Med4 as a Gatekeeper of Metastasis through Epigenetic Control of Integrin Signaling
bioRxiv
November 23, 2023
Seong-Yeon Bae, Yi Chen, Hong Chen, Dhiraj Kumar, Spyros Karaiskos, Jane Xu, Chao Lu, Aaron D. Viny, Filippo G. Giancotti
35 s), and 72°C for 1 min).
PCR
was performed using
NEBNext® High-Fidelity 2X PCR Master Mix
(
NEB
;
M0541S
). The purified
PCR
products were separated by a 4–20% TBE gel (
Invitrogen
;
EC62252BOX
) and stained with
(9)
Intrinsic epigenetic state of primary osteosarcoma drives metastasis
bioRxiv
November 17, 2023
Irtisha Singh, Nino Rainusso, Lyazat Kurenbekova, Bikesh K. Nirala, Juan Dou, Abhinaya Muruganandham, Jason T. Yustein
purified DNA was then pre-amplified for 5
PCR
Cycles using NEBNext
High-Fidelity PCR
MasterMix (
NEB
,
M0541
) and Illumina indexing primers. To define the final number of
amplification
cycles required, the pre-amplified
(10)
Unistrand piRNA clusters are an evolutionarily conserved mechanism to suppress endogenous retroviruses across the Drosophila genus
Nature Communications
November 13, 2023
Jasper van Lopik, Azad Alizada, Maria-Anna Trapotsi, Gregory J. Hannon, Susanne Bornelöv, Benjamin Czech Nicholson
amplified for 5 cycles using the NEBNext High-Fidelity MasterMix (
New England BioLabs
; catalogue nr.
M0541S
) followed by
qPCR
amplification
to determine the exact number of additional cycles required for optimal
(11)
Dapagliflozin alleviates renal inflammation and protects against diabetic kidney diseases, both dependent and independent of blood glucose levels
Frontiers in Immunology
November 9, 2023
Anxiang Cai, Jianxiao Shen, Xiaoqian Yang, Xinghua Shao, Leyi Gu, Shan Mou, Xiajing Che
five cycles of pre-
amplification
using
NEBNext High-Fidelity 2X PCR Master Mix
(
New England Biolabs
,
M0541S
). To determine the suitable number of cycles required for the second round of
PCR
, the library was assessed
(12)
Joint sequence & chromatin neural networks characterize the differential abilities of Forkhead transcription factors to engage inaccessible chromatin
bioRxiv
November 5, 2023
Sonny Arora, Jianyu Yang, Tomohiko Akiyama, Daniela Q. James, Alexis Morrissey, Thomas R. Blanda, Nitika Badjatia, William K.M. Lai, Minoru S.H. Ko, B. Franklin Pugh,
et al.
1 × SYBR Green (Invitrogen), custom-designed primers [69], and 2 ×
NEB
MasterMix (New England Labs,
M0541
). The library was cleaned up with AMPure XP beads and the fragment length distribution of the library
(13)
Deciphering the mitophagy receptor network identifies a crucial role for OPTN (optineurin) in acute myeloid leukemia
Autophagy
November 1, 2023
Meyer LM, Koschade SE, Vischedyk JB, Thoelken M, Gubas A, Wegner M, Basoglu M, Knapp S, Kaulich M, Eimer S,
et al
.
of each 10 µM PCR1 forward and reverse primers and 25 µL Next High-Fidelity 2× PCR Master Mix (
NEB
,
M0541L
) was used. Thermal cycler protocol was applied as follows: Initial denaturation at 98°C for 2 min, 15
(14)
Myeloid leukemia vulnerabilities embedded in long noncoding RNA locus MYNRL15
iScience
October 20, 2023
Michelle Ng, Lonneke Verboon, Hasan Issa, Raj Bhayadia, Marit Willemijn Vermunt, Robert Winkler, Leah Schüler, Oriol Alejo, Konstantin Schuschel, Eniko Regenyi,
et al.
DNA blood mini kit,
Qiagen
,Cat#
51104
NEBNext® high-fidelity PCR master mix,
New England Biolabs
,Cat#
M0541L
Human methylcellulose complete media
,
R&D Systems
,Cat#
HSC003
Dual-luciferase® reporter assay system
,
Promega
,Cat#
(15)
KMT2D suppresses Sonic hedgehog-driven medulloblastoma progression and metastasis
iScience
October 20, 2023
Reeti Mayur Sanghrajka, Richard Koche, Hector Medrano, Salsabiel El Nagar, Daniel N. Stephen, Zhimin Lao, N. Sumru Bayin, Kai Ge, Alexandra L. Joyner
Buffer,Illumina,15027866 Qiagen MinElute Reaction Cleanup Kit,
Qiagen
,
28204
NEBNext High-Fidelity 2X PCR Master Mix
,
NEB
,
M0541S
SYBR Green I,
Thermo Fisher Scientific
,
S7563
Agencourt AMPure XP magnetic beads,
Beckman Coulter
,
A63880
Agilent
(16)
Inflammatory stress-mediated chromatin changes underlie dysfunction in endothelial cells
bioRxiv
October 20, 2023
Haibo Liu, Amada D. Caliz, Heather Learnard, Milka Koupenova, John F. Keaney, Shashi Kant, Lihua Julie Zhu, Anastassiia Vertii
Libraries were prepared from purified transposed DNA using NEBNext
High-Fidelity PCR
MasterMix (
NEB
,
M0541
) and Illumina indexing primers. The number of
library amplification
cycles was determined by
qPCR
analysis
(17)
Antioxidants stimulate BACH1-dependent tumor angiogenesis
The Journal of clinical investigation
October 16, 2023
Wang T, Dong Y, Huang Z, Zhang G, Zhao Y, Yao H, Hu J, Tüksammel E, Cai H, Liang N,
et al
.
(
Zymo Research
,
D4013
). IDT primers (Illumina, 20027213) and
PCR
enzyme mix (New England BioLabs [
NEB
],
M0541S
) were used for library preparation, and AMPure beads (
Beckman Coulter
,
A63881
) were used for
PCR
cleanup.
(18)
Guide-specific loss of efficiency and off-target reduction with Cas9 variants
Nucleic Acids Research
October 13, 2023
Liang Zhang, Wei He, Rongjie Fu, Shuyue Wang, Yiwen Chen, Han Xu
pairs listed in Supplementary Table S2 with 16 cycles using NEBNext High Fidelity Master Mix (
NEB
, #
M0541
) for the screens with EpiC and Tiling libraries and
NEBNext Ultra II Q5 Master Mix
(
NEB
, #M0544) for
(19)
A transcriptional and regulatory map of mouse somite maturation
Developmental Cell
October 9, 2023
Ximena Ibarra-Soria, Elodie Thierion, Gi Fay Mok, Andrea E. Münsterberg, Duncan T. Odom, John C. Marioni
proteins","Chemicals, peptides, and recombinant proteins" ,, NEB Next High Fidelity 2X PCR Master Mix,
NEB
,Catalog #
M0541L
EvaGreen Dye 20X in water,Biotum,Catalog #31000 Agencourt AMPure XP Beads,"
Beckman Coulter, Inc
.",Cat.
(20)
Sex-associated early-life viral innate immune response is transcriptionally associated with chromatin remodeling of type-I IFN-inducible genes
Mucosal immunology
October 1, 2023
Malinczak CA, Fonseca W, Mire MM, Parolia A, Chinnaiyan A, Rasky AJ, Morris S, Yagi K, Bermick JR, Lukacs NW.
column and
PCR
-amplified for 12 cycles with the
NEBNext High-Fidelity 2X PCR Master Mix
(
NEB
; cat. no.
M0541L
).
qPCR
was used to determine the optimal
PCR
cycles to prevent over-
amplification
. The amplified library
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NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs
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