NEBNext High-Fidelity 2X PCR Master Mix M0541S from New England Biolabs

Product Specs
ItemNEBNext High-Fidelity 2X PCR Master Mix
CompanyNew England Biolabs
Price $92.00 Inquire
Catalog NumberM0541S
Size50 reactions
ApplicationsDNA Analysis, PCR
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol update to: Genome-wide interrogation of protein-DNA interactions in mammalian cells using ChIPmentationSTAR ProtocApril 16, 2026Xuemei Li, Wei Xu, Ying Ye, Andrew D. Sharrocks, Wensheng Zhang, Xi Chen252549 Glycine,Sigma,G8790 20X EvaGreen,Biotium,Cat# 31000-T NEBNext High-Fidelity 2X PCR Master Mix,NEB,M0541 Dynabeads™ Protein A for Immunoprecipitation,ThermoFisher,10001D Dynabeads™ Protein G for Immunoprecipitation,ThermoFisher,10003D Dynabeads™ Pan Mouse IgG

(2) STAG2 loss amplifies EWS-FLI1-driven microsatellite enhancer activity promoting Ewing sarcoma aggressivenessProc Natl Acad Sci U S AApril 14, 2026Sanjana Eyunni, Shih-Chun Chu, Mary L. Guan, Michaela Louw, Eleanor Young, Sandra E. Carson, Jianhui Gong, Marcin Cieslik, Arul M. Chinnaiyan, Abhijit Paroliacolumn, and sequencing adapters were enriched by PCR using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L), with the number of amplification cycles empirically determined by qPCR to prevent overamplification.

(3) CUT&Tag for High-Resolution Epigenomic Profiling From a Low Amount of Arabidopsis TissuePlant DirectApril 10, 2026Yixuan Fu, Marc W. Schmid, Sara Simoninitagmented DNA, 2 μL i5 primer (10 μM) and 2 μL i7 primer (10 μM), and 25 μL NEBNext Hi‐Fi 2× PCR mix (NEB #M0541) to make a total final volume of 50 μL. Make sure to use unique combinations of i5 and i7 primers for

(4) Sp1 mechanotransduction regulates breast cancer cell invasion in engineered viscoelastic extracellular matricesBiomaterialsApril 1, 2026Abhishek Sharma, Rowan F. Steger, Jen M. Li, Samuel Y. Fong, Neha Saxena, Jane A. Baude, Kellie A. Heom, Siddharth S. Dey, Ryan S. Stowersi5 indexed primers, dNTPs (New England Biolabs, Cat# N0447l), and PCR master mix (New England, Cat# M0541S). The size distribution of the libraries was quantified on an Agilent Bioanalyzer 2100 and Qubit fluorometer.

(5) NFYA regulates two sequential genome-wide transcriptional activation events during oocyte to embryo transitionbioRxivApril 1, 2026Qianying Yang, Shan Jiang, Boyan Wang, Yi Zhangto quench SDS. Sequencing libraries were prepared with NEBNext High-Fidelity 2×PCR Master Mix (NEB, M0541S). RNA-seq library was prepared by SMART-Seq® Stranded Kit (Takara Bio, 63444) using 5,000 mESCs, 20~50

(6) ECHOS enables spatial epigenome profiling at subcellular resolutionbioRxivMarch 31, 2026Qiqi Cao, Qianlan Xu, Yusuke Ueda, Shreya Rajachandran, Manjita Sharma, Xin Zhang, Mala Mahendroo, Edward J. Grow, Haiqi Chenat 50 °C for 30–60 min, followed by second-strand synthesis (SSS) using NEBNext 2× master mix (NEB, M0541L) and SSS primer (ECHOS_T7split_SSS) to generate double-stranded cDNA. The cDNA was used to generate

(7) CLOCK is an Epigenetic Integrator of Circadian Rhythm and T Cell ImmunityResearch SquareMarch 27, 2026Khashayarsha Khazaie, Shirin Eyvazi, Abdulrahman Saadalla, Mahendra Pal Saadalla, Mahendra Pal Marlow, Cameron Keshavarzian, Faraz Keshavarzian, Faraz Bishehzari, Aleksey Matveyenko, Aleksey Matveyenko, et al.amplified by PCR using Illumina Nextera Index primers and NEBNext PCR Master Mix (New England BioLabs, M0541). Final libraries were cleaned using a Qiagen PCR purification kit and sequenced on the Illumina HiSeq

(8) Krüppel-like factors 2 and 3 regulate T cell exhaustion by directing T cell residency and migrationImmunityMarch 27, 2026Jian Shen, Ryan J. Brown, Yuqi Zhang, Kexin Gai, Ashley K. Brown, Ashley M. Bauer, Ashley Zhu, Siying Lin, Hongshen Niu, Arjun Kharel, et al.paramagnetic beads,EpiCypher,Cat# 21-1411 NEBNext High-Fidelity 2X PCR Master Mix,New England Biolabs,Cat# M0541L QIAprep Spin Miniprep Kit,Qiagen,Cat# 27106 DNeasy Blood & Tissue Kit,Qiagen,Cat# 69504 MinElute

(9) Characterization of HER2-Positive Murine Breast Cancer Models for Investigating HER2-Targeted Therapy and ImmunotherapyCancers (Basel)March 19, 2026Yun Lu, Benjamin P. Lee, Abbigael V. Eli, Shannon E. Lynch, Ar Rafi Md Faisal, Jonathan Moye, Anna G. Soracevariant were PCR-amplified with the NEBNext High-Fidelity 2X PCR master mix (NEB, Ipswich, MA, USA, M0541S) in 50 μL total volume using the following primers: forward 5′-ACTGTCTAGAATGGAGCTGGCGGCCTTGT-3′ and

(10) Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fateNucleic Acids ResMarch 19, 2026Patrizia Maria Christiane Nothnagel, Paul-Arthur Meslin, Jonas Aakre Wik, Damien Dufour, Yunna Erika Strøm, Magnar Bjørås, Jorrit Martijn Enserink, Bjørn Steen Skålhegg, Nolwenn Briand, Anthony Mathelier, et al.[Ad2.(1–15)] (Supplementary Table S1 and [57]) and 25 μl NEB Next High-Fidelity 2× PCR Master Mix (NEB, M0541S) and the following thermal cycle settings: 1 cycle: 72°C for 5 min; 1 cycle: 98°C for 30 s; 13 cycles

(11) Systematic Evaluation Defines the Limits of Ferroptosis in Cancer TherapybioRxivMarch 17, 2026Kenji M. Fujihara, Azmi Aziz, Behnia Akbari, Maria Gutierrez-Perez, Gregory Francis, Siham Zentout, Kameng Wu, Nicholas J. Clemons, Erdem M. Terzi, Michael E. Pacold, et al.in at least four parallel 50 μL reactions containing 25 μL NEBNext High-Fidelity 2× PCR Master Mix (M0541L, New England Biolabs), 1 μL oligonucleotide pool (5 ng), 2.5 μL each of 10 μM forward and reverse primers,

(12) A functional overlap between actively transcribed genes and chromatin insulator elementsThe EMBO JournalMarch 16, 2026Lucy J Cornell, Caroline L Harrold, Susannah Holliman, Felice Tsang, Matthew E Gosden, Lars L P Hanssen, Rosa Stolper, Damien J Downes, Daniel Biggs, Chris Preece, et al.,New England Biolabs,E7645S Tagment DNA Enzyme Kit,Illumina,20034211 NEBNext High-Fidelity 2x PCR Master Mix,New England Biolabs,M0541 NEBNext rRNA Depletion Kit (Human/Mouse/Rat),New England Biolabs,E6310 rRNA and globin mRNA using

(13) ADAMTS7 promotes smooth muscle foam cell expansion in atherosclerosisJournal of Clinical InvestigationMarch 16, 2026Allen Chung, Lauren E. Fries, Hyun-Kyung Chang, Huize Pan, Alexander C. Bashore, Karissa Shuck, Caio V. Matias, Juliana Gomez Pardo, Jordan S. Kesner, Hanying Yan, et al.Kit. Transposed DNA was amplified using NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs, M0541) with Nextera indexing primers, and libraries were purified with AMPure XP magnetic beads (Beckman Coulter,

(14) H3K4me3 CUT&Tag and Transcriptome Analysis Reveal the Epigenetic Regulatory Landscape in Mammary Gland Tissues of Yili Horses at Different Lactation StagesAnimals (Basel)March 12, 2026Lingling Liu, Hang Cao, Haiyu Ma, Bin Chen, Wujun Liu(Vazyme, Cat. No. TD203, Nanjing, China). A total of 25 µL of NEBNext HiFi 2× PCR Master Mix (Cat. No. M0541, NEB, Ipswich, MA, USA) was added and thoroughly mixed. PCR amplification was performed using the following

(15) CEBPB-regulated gastric cell plasticity promotes liver metastasis of gastric cancerCancer CommunicationsMarch 12, 2026Zhixiang Zuo, Jiaqi Liang, Li-Na He, Kai Xu, Muren Hu, Kunming Zhang, Wei Gao, Junyi Yin, Lanlin Zhang, Boning Ma, et al.Buenrostro et al. [35], with 8 to 11 cycles of PCR using NEBNext High Fidelity 2× PCR master mix (Cat. #M0541S, New England Biolabs, Inc.). The number of PCR cycles was empirically determined for each library by

(16) Transcript-guided targeted cell enrichment for scalable single-nucleus RNA sequencingCell GenomicsMarch 11, 2026Andrew Liao, Zehao Zhang, Andras Sziraki, Abdulraouf Abdulraouf, Abid Rehman, Zihan Xu, Ziyu Lu, Weirong Jiang, Alia Arya, Jasper Lee, et al.Maxima H Minus Reverse Transcriptase (200 U/μL),Thermo Scientific,Cat#EP0752 NEBNext High-Fidelity 2× PCR Master Mix,NEB,Cat#M0541L NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module,NEB,Cat#E6111L Penicillin-Streptomycin,Gibco,Cat#15140122 SUPERase⋅In™

(17) Comparison with Ezh2 reveals the PRC2-dependent functions of Jarid2 in hematopoietic stem Cell lineage commitmentStem Cell ReportsMarch 10, 2026Hassan Bjeije, Wentao Han, Shuyang Lin, Nancy Issa, Aishwarya Krishnan, Infencia Xavier Raj, Jason Arand, Yanan Li, Wei Yang, Jeffrey A. Magee, et al.PCR amplification using specific i5 and i7 index primers and the NEBNext High-Fidelity 2× PCR Master Mix (Cat# M0541S, New England BioLabs). Library size selection was performed using SPRIselect beads (Cat# B23318, Beckman

(18) Mechanisms of gene regulation by SRCAP and H2A.ZNature CommunicationsMarch 6, 2026Armelle Tollenaere, Enes Ugur, Susanna Dalla Longa, Cédric Deluz, Devin Assenheimer, J. Christof M. Gebhardt, Heinrich Leonhardt, David M. SuterTransposed DNA was amplified in a solution containing 1X NEBNext High Fidelity PCR Master Mix (NEB #M0541L), 0.5 μM of Ad1.1 universal primer, 0.5 μM of Ad2.x indexing primer, and 0.6x SYBR Green I (Thermo Fisher Scientific

(19) Integrative Multi-omics Analysis of the Human Skeletal Muscle Response to Endurance or Resistance Exercise: Findings from the Molecular Transducers of Physical Activity Consortium (MoTrPAC)bioRxivMarch 6, 2026Hasmik Keshishian, Gina M. Many, Gregory Smith, Natalie M. Clark, Gayatri Iyer, Patrick Hart, Malene E. Lindholm, Samuel Montalvo, Zidong Zhang, Christopher Jin, et al.(Zymo research, D4014). The DNA product was amplified using NEBnext High-Fidelity 2x PCR Master Mix (NEB, M0541L) and custom indexed primers. Purified PCR libraries were amplified using Ampure XP beads, double-sided

(20) An endothelial RNA splicing atlas catalogs effects of IL-1β and identifies an alternative PROCR isoform with genetic links to pleiotropic vascular diseaseThe American Journal of Human GeneticsMarch 5, 2026Anna K. Golebiewski, Lindsey K. Stolze, Valentina D. Vazquez, Alhan Mehrabi Yazdi, Cecilia M. Careaga, Casey E. Romanoski(annealing temperatures) are listed in Table S1. PCR was performed using the NEB2Next PCR Master Mix (NEB # M0541L) with the following cycling conditions: 1) 98°C for 30 seconds, 2) 98°C for 1- seconds, 3) 60–63°C for

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NEBNext High-Fidelity 2X PCR Master Mix

NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs

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NEBNext High-Fidelity 2X PCR Master Mix from New England Biolabs

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