Q5 Hot Start High-Fidelity 2X Master Mix M0494S from New England Biolabs

Product Specs
ItemQ5 Hot Start High-Fidelity 2X Master Mix
CompanyNew England Biolabs
Price $233.00 Inquire
Catalog NumberM0494S
Size100 reactions
ApplicationsDNA Analysis
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) KOLF2.1J iTF-Microglia: A standardized platform to study microglial transcriptional regulatory networks in CNS diseaseiScienceJuly 17, 2026Brianne B. Rogers, Ashlyn G. Anderson, Ivan Rodriguez-Nunez, Samuel C. Bartley, S. Quinn Johnston, Jared W. Taylor, Sarah K. Meadows, Kimberly M. Newberry, Richard M. Myers, J. Nicholas CochranKit,Norgen,17270 Tagment DNA TDE1 Enzyme and Buffer,Illumina,20034197 Q5 HotStart Master Mix,NEB,M0494S Arima-HiC+ Genome-Wide Hi-C Kit,Arima Genomics,A410232 ,, Deposited data,Deposited data,Deposited

(2) Confined migration induces non-lethal DNA damage in developing neuronsNatureJune 17, 2026Zhejing Zhang, Andres Canela, Junko Kurisu, Peilin Zou, Takumi Kawaue, Naotaka Nakazawa, Noriko Takeda, Mai Saeki, Masaki Utsunomiya, Merve Bilgic, et al.ATAC DNA was used for library amplification using the Q5 Hot Start High-Fidelity 2× Master Mix (NEB, M0494S). The libraries were sequenced on the NextSeq 500 platform (Illumina) using 75 bp single-end reads. END-seq Dissociated

(3) Mitochondrial genome microhomology-mediated editing by donor DNA delivery into mitochondria in human cellsMolecular Therapy Nucleic AcidsJune 16, 2026Vadim V. Maximov, Nikita Shebanov, Natalia Nikitchina, Rachel Rapoport, Yehoshua Maor, Ivan Tarassov, Ophry Pines, Nina Enteliswas 0.02 μM. The total reaction volume was 10 μL. Q5 Hot Start High-Fidelity 2× Master Mix (cat. # M0494, New England Biolabs) was used for this reaction. The reaction conditions were as follows: 98°C – 2

(4) CTCF directly binds G-quadruplex structures to regulate genome topology and gene expressionProceedings of the National Academy of SciencesJune 2, 2026Daniela Samaniego-Castruita, Isabella Han, Roxroy C. Morgan, Samantha Carpenter, Bryce Williams, Abhijit Chakraborty, Ishwar Radhakrishnan, Ferhat Ay, Samuel A. Myers, Anjana Rao, et al.with forward and reverse primers (each 10 μM) and Q5 Hot Start High-Fidelity 2X Master Mix (NEB Cat# M0494S) for amplification. Following the PCR amplification, the product was subjected to a Kinase, Ligase,

(5) An ancient retrotransposon provides species-specific tuning of IL-18 inflammatory signalingbioRxivMay 29, 2026Andrea D. Ordonez, Holly Allen, Lynn Sanford, Atma Ivancevic, Ashley Agyepong, Mia Chaw, James P. Bridges, Tony Schountz, Edward B. ChuongIL-18 pathway receptors and human IL18BP were amplified using Q5 High-Fidelity DNA Polymerase (NEB; M0494L), whereas human IL18R1 receptors, human IL18RAP, and Jamaican fruit bat IL18R1-Short were synthesized

(6) Generative modeling for RNA splicing prediction and designElifeMay 29, 2026Di Wu, Natalie Maus, Anupama Jha, Kevin Yang, Benjamin D Wales-McGrath, San Jewell, Anna Tangiyan, Peter Choi, Jake R Gardner, Yoseph Barashand PCR for the splicing event was performed with the Q5 Hot Start High-Fidelity 2 X Master Mix (NEB, M0494S). PCR reactions were then visualized on a 2% agarose gel in 1 X lithium boric acid buffer (Faster Better Media

(7) Microtubule-templated actin assembly by septin9 drives apical expansion of epithelial cellsbioRxivMay 23, 2026Angelo Arrigo, Victoria Hua, Elias T. Spiliotis, Saurabh Kulkarni,Selleck Chemicals,S2775 Latrunculin A,Sigma,428021 Q5 Hot Start High-Fidelity 2X Master Mix,New England Biolabs,M0494S Gibson Assembly Master Mix,New England Biolabs,E2611S NEBuilder HiFi DNA Assembly Cloning Kit,New England Biolabs

(8) Modulation of WNT and FGF18 enhances yield and subtype identity of hPSC-derived midbrain dopamine neuronsJ Clin InvestMay 15, 2026Tae Wan Kim, Jinghua Piao, Vittoria D. Bocchi, So Yeon Koo, Se Joon Choi, Fayzan Chaudhry, Donghe Yang, Hyein S. Cho, Emiliano Hergenreder, Lucia Ruiz Perera, et al.targeting constructs were generated from a genomic DNA PCR with Q5 polymerase (New England Biolabs #M0494) amplifying ~500bp of homology per side and assembled with NEBuilder (New England Biolabs #E2621S).

(9) The Roles of MKI67, GNL2, and MDN1 in Ribosome Biogenesis and Transcriptome Regulation in the Neuronal Cell Line HEK293TbioRxivMay 5, 2026Shiro Iuchi, Joao A. Paulothe cDNA encoding GNL2 (Q13823) was amplified using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0494S). This product was then cloned into the ECORI-SCAII site of pEGFP-C2, yielding pGNL2-G7, which encodes

(10) Reciprocal repulsions enforce heterotypic dendrite segregation in an olfactory circuitbioRxivMay 4, 2026Hui Ji, Jingxian Li, Yizhen Xu, Kenneth Kin Lam Wong, Yunming Wu, David J. Luginbuhl, Yanbo Zhang, Zhuoran Li, Jaeyoon Lee, Robert C Jones, et al.assays,Critical commercial assays Q5 hot-start high-fidelity DNA polymerase,New England Biolabs,Cat# M0494S NEBuilder HiFi DNA assembly master mix,New England Biolabs,Cat# E2621L Deposited data,Deposited data,Deposited

(11) A fast-to-faithful transition shapes the DNA repair landscape during embryogenesisbioRxivMay 1, 2026Shengzhou Wang, Ian Pantziris, Mengsheng Zhang, Yang Liu, James A. Gagnonsamples were amplified with PCR using Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs M0494). PCR amplification as performed with the following conditions: 98 °C for 30 seconds, 25 cycles (tyr),

(12) THE FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycleeLifeApril 30, 2026Taylar Hammond, Jong Bin Choi, Miles W Membreño, Janos Demeter, Roy Ng, Debadrita Bhattacharya, Thuyen N Nguyen, Griffin G Hartmann, Caterina I Colón, Carine Bossard, et al.for FAM53C was amplified by PCR using Q5 Hot Start High-Fidelity 2 X Master Mix (New England Biolabs, M0494S). To select the FAM53C knockout clone, we used the following primer pair: forward, 5′-GGCCCAAGATTCCTCTCGAC-3′,

(13) An anaerobic pathogen rewires host metabolism to fuel oxidative growth in the inflamed gutCellApril 30, 2026Luisella Spiga, Ryan T. Fansler, Yifan Wu, Alexandra Grote, Madison Langford-Butler, Asia K. Miller, Maxwell Neal, Owen F. Hale, Deepanshu Singla, M. Wade Calcutt, et al.Kit,Qiagen,47014 PowerUp SYBR Green Master Mix,Applied Biosystems,A25742 Q5 Hot Start 2x Master Mix,NEB,M0494L Quick-DNA Fungal/Bacterial Miniprep Kit,Zymo Research,D6005 RNAlater™ Stabilization Solution,Invitrogen,AM7020 RNeasy

(14) The FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycleElifeApril 30, 2026Taylar Hammond, Jong Bin Choi, Miles W Membreño, Janos Demeter, Roy Ng, Debadrita Bhattacharya, Thuyen N Nguyen, Griffin G Hartmann, Caterina I Colon, Carine Bossard, et al.for FAM53C was amplified by PCR using Q5 Hot Start High-Fidelity 2 X Master Mix (New England Biolabs, M0494S). To select the FAM53C knockout clone, we used the following primer pair: forward, 5′-GGCCCAAGATTCCTCTCGAC-3′,

(15) Nitrogen metabolism profiling reveals cell state-specific pyrimidine synthesis pathway choiceNature MetabolismApril 29, 2026Milan R. Savani, Bingbing Li, Bailey C. Smith, Wen Gu, Yi Xiao, Gerard Baquer, Tracey Shipman, Skyler S. Oken, Namya Manoj, Lauren G. Zacharias, et al.was amplified by PCR with Q5 Hot Start High-Fidelity 2× Master Mix polymerase (New England Biolabs, M0494). A cDNA library prepared from NHA donor #1 cells with the RNeasy Mini kit (QIAGEN, 74004) and LunaScript RT Supermix

(16) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrowElifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livak, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,Propidium iodide,Sigma-Aldrich,P4170, "Chemical compound, drug",Q5 Hot Start High-Fidelity 2X Master Mix,NEB,M0494S, "Chemical compound, drug",Richard-Allan Scientific Cover glass,Epredia,102424, "Chemical compound,

(17) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,Propidium iodide,Sigma-Aldrich,P4170, "Chemical compound, drug",Q5 Hot Start High-Fidelity 2X Master Mix,NEB,M0494S, "Chemical compound, drug",Richard-Allan Scientific Cover glass,Epredia,102424, "Chemical compound,

(18) Ribosome Molecular Aging Shapes Translation DynamicsbioRxivApril 25, 2026Jordy F. Botello, Lifei Jiang, Peter J. Metzger, Troy J. Comi, Aya A. Abu-Alfa, Qiwei Yu, Margaret S. Ebert, Minkyu Lee, Lennard W. Wiesner, Maya Butani, et al.DNA fragments were amplified from original plasmids by PCR using Q5 High-Fidelity 2X Master Mix (NEB, M0494L) with oligonucleotides synthesized by IDT, then the In-Fusion HD Cloning kit (Takara, 638949) was used

(19) Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contextsmedRxivApril 24, 2026Shawn Fayer, Riddhiman K. Garge, Melissa Hopkins, Clayton E. Friedman, Abby V. McGee, Joshua Rico, Rachel L. Powell, Evan McDermot, Nahum T. Smith, Sriram Pendyala, et al.MYBPC3 allele specific PCR was conducted with Q5 Hot Start High-Fidelity 2x Master Mix (NEB; cat. no. M0494S). POLG allele specific PCR was performed with LongAmp Taq DNA Polymerase (NEB; cat. no.M0323S). gDNA

(20) Disruption of Cell-Type-Specific Molecular Programs of Medium Spiny Neurons in AutismbioRxivApril 23, 2026Guohua Yuan, Varun Suresh, Emilie Wigdor, Yuhan Hao, Rachel Leonard, Marilyn Steyert, Michael Griffiths, Clements Evans, Narjes Rohani, Jakob Weiss, et al.were subamplified from each 10X cDNA library using the Q5 Hot Start High Fidelity 2× master mix (NEB, M0494). In brief, 10 μl of cDNA was used as template in a 50 μl PCR reaction containing STICR barcode read

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(1)

10/6/2023Convenient PCR Master Mix From New England Biolabs Anh NguyenI used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)

3/31/2023Good PCR Master Mix YONG MTry this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)

1/2/2020DNA Insert Amplification for CloningDr. Thippeswamy SannasiddappaHighly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.

Q5 Hot Start High-Fidelity 2X Master Mix

Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

New England Biolabs

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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

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