Q5 Hot Start High-Fidelity 2X Master Mix M0494S from New England Biolabs

Product Specs
ItemQ5 Hot Start High-Fidelity 2X Master Mix
CompanyNew England Biolabs
Price $233.00 Inquire
Catalog NumberM0494S
Size100 reactions
ApplicationsDNA Analysis
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) THE FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycleeLifeApril 30, 2026Taylar Hammond, Jong Bin Choi, Miles W Membreño, Janos Demeter, Roy Ng, Debadrita Bhattacharya, Thuyen N Nguyen, Griffin G Hartmann, Caterina I Colón, Carine Bossard, et al.for FAM53C was amplified by PCR using Q5 Hot Start High-Fidelity 2 X Master Mix (New England Biolabs, M0494S). To select the FAM53C knockout clone, we used the following primer pair: forward, 5′-GGCCCAAGATTCCTCTCGAC-3′,

(2) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,Propidium iodide,Sigma-Aldrich,P4170, "Chemical compound, drug",Q5 Hot Start High-Fidelity 2X Master Mix,NEB,M0494S, "Chemical compound, drug",Richard-Allan Scientific Cover glass,Epredia,102424, "Chemical compound,

(3) Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunityNat CommunApril 20, 2026Kotaro Chihara, Aa Haeruman Azam, Artyom A. Egorov, Ilya Terenin, Masanori Hashino, Koichi Watashi, Kazuhiro Horiba, Vasili Hauryliuk, Kotaro Kigaresuspended in 10 mM Tris-HCl (pH 8.5). Bead-bound DNA was amplified using Q5 Hot-Start Master Mix (NEB, #M0494S) and Recovery PCR primers in 50 µL reactions. Following thermal cycling, supernatants were recovered

(4) Rapid and precise amplicon-based genome sequencing to preparedness and response chikungunya virus outbreaksEmerg Microbes InfectApril 15, 2026Kaiying Wang, Jinhui Li, Tianyuan Liu, Peihan Li, Ning Wang, Yuqi Liu, Yufeng Liang, Fenxiang Li, Chuhong Wei, Huacheng Yan, et al.by multiple amplification. The amplification system included Q5® High-Fidelity 2X Master Mix (NEB #M0494) 6.25 µL, cDNA 4.5 µL and primer pool 1/2 1.75 µL. The pool 1 and pool 2 need to be amplified separately.

(5) ECHOS enables spatial epigenome profiling at subcellular resolutionbioRxivMarch 31, 2026Qiqi Cao, Qianlan Xu, Yusuke Ueda, Shreya Rajachandran, Manjita Sharma, Xin Zhang, Mala Mahendroo, Edward J. Grow, Haiqi Chenbuffer at 22 °C for 1 h. After ligation, PCR was performed using Q5 High-Fidelity DNA polymerase (NEB, M0494L) by following the program: 72°C for 5min, 98°C for 30 s; 5 cycles of 98°C for 10 s and 72°C for 90 s,

(6) Regulated transformation system (RTS): sddi-mediated programmable shut-off and mode switching of base editorsNucleic Acids ResMarch 30, 2026Jiacheng Deng, Jian Zhou, Hongyong Xiang, Xueyuan Li, Xiang Han, Zhen Weng, Junbo Jia, Yingshuo Shao, Yang Sima, Ming Niu, et al.Target sites were PCR-amplified using the Q5 High-Fidelity 2X Master Mix (New England Biolabs, USA, M0494S). Amplicons were then submitted to the Hi-TOM [36] (http://www.hi-tom.net/hi-tom/index-CH.php). Prediction

(7) A self-complementary recombinant adeno-associated virus vector coding for an anchorless prion protein carrying the G127V mutation extends survival in a rodent prion disease modelbioRxivMarch 30, 2026Thomas Zerbes, Claire Verkuyl, Cunjie Zhang, Sophie Grunnesjoe, Shehab Eid, Hamza Arshad, Wenda Zhao, Zahra Nasser, Teaghan O’Shea, Ari Belotserkovsky, et al.reaction, 50 ng of gDNA was amplified with Q5 Hot Start High-Fidelity 2X Master Mix (catalog number M0494S, New England Biolabs) and forward and reverse primers (final concentration 500 nM) that map to gDNA

(8) Cytoplasmic mRNA decay by the antiviral nuclease RNase L promotes transcriptional repressionCell ReportsMarch 24, 2026Xiaowen Mao, Sherzod Tokamov, Felix Pahmeier, Azra Lari, Jinyi Xu, Eva Harris, Britt Glaunsingerindicated in the key resources table. All PCR reactions were performed using Q5 2X master mix (NEB, cat #M0494S). Sequences encoding the CRS tag, HALO tag, and RNase L with appropriate overhangs were PCR amplified.

(9) Protocol for validating computationally predicted splice-altering variants using full-length gene reporter assaysSTAR ProtocMarch 20, 2026Maria E. Lukes, Kostantin Kiianitsa, Anthony Widjaja, Olena KorvatskaMix,New England Biolabs,Cat# M0482L Q5 Hot Start High-Fidelity 2× Master Mix,New England Biolabs,Cat# M0494S Luna Universal qPCR Master Mix,New England Biolabs,Cat# M3003L Amaxa SF Cell Line X Kit S,Lonza,Cat#

(10) The Rpd3 histone deacetylase is a critical regulator of temperature-mediated morphogenesis and virulence in the human fungal pathogen HistoplasmaPLOS BiologyMarch 17, 2026Nebat Ali, Mark Voorhies, Rosa A. Rodriguez, Anita Silgenerated through amplifying the RPD3 open reading frame (ORF) from genomic DNA using Q5 Polymerase (NEB M0494S) and primers (see S3 Table) overlapping with a pDONR overexpression backbone amplified from pDI71. The

(11) DNA secondary structures in BCL2 and MYC elicit activation-induced cytidine deaminase binding and activityNucleic Acids ResMarch 14, 2026Mason McCrury, Ambrocio Sanchez, Rylie Mangold, Todd J Spears, Claire Doshier, Rémi Buisson, Mojnu Miah, Derin Akdeniz, Samantha KendrickESI-MS. Substitutions and insertions into plasmid DNA were performed using Q5 HotStart 2× Mix (catalog # M0494), T4 polynucleotide kinase (catalog # M0201), T4 ligase (catalog # M0202), and DpnI (catalog # R0176)

(12) The yeast phosphofructokinase β-subunit has RNA unwinding activity and modulates cell cycle progressionNucleic Acids ResMarch 12, 2026Waleed S Albihlal, Ana M Matia-González, Tobias Schmidt, Wieland Mayer, Joe Bryant, Martin Bushell, Dierk Niessing, Matteo Barberis, Alexander Schmidt, Jürgen J Heinisch, et al.NXA931). Kits (in order of appearance): Q5® Hot Start High-Fidelity 2× Master Mix (New England Biolabs, M0494S), QIAquick polymerase chain reaction (PCR) Purification Kit (Qiagen, 28104), Kinase-Ligase-DpnI (KLD) enzyme mix

(13) Bacillus subtilis MutL samples multiple conformations during nucleotide binding and hydrolysisStructureMarch 5, 2026Javier Rodríguez Rodríguez González, Corey L. Davis, Hunter Wilkins, Dorothy A. Erie, Alba Guarnéassays,Critical commercial assays,Critical commercial assays Q5® hot start high-fidelity 2x master mix,NEB,Cat# M0494S Deposited data,Deposited data,Deposited data N-terminal domain of Bacillus subtilis MutL bound to

(14) Retrospective Molecular Detection and Characterization of Pathogenic Leptospira in the PhilippinesTrop Med Infect DisMarch 4, 2026Joanna Ina G. Manalo, Adeliza Mae L. Realingo, Lei Lanna M. Dancel, Timothy John R. Dizon, Amalea Dulcene Nicolasora, Kristine Alvarado-Dela Cruz, Desiree D. Argana, Arjay Niño A. Digman, Emarld Julian G. Medina, Celine Bernice A. Roxas, et al.Boonsilp assay for molecular detection, using the Q5® Hot Start High-Fidelity 2X Master Mix (Catalog No. M0494L, New England Biolabs, Ipswich, MA, USA) and a C1000 PCR Thermal Cycler (Bio-Rad, Hercules, CA, USA).

(15) Genomic factors contributing to the resilience of Salmonella enterica on ready-to-eat muskmelonFood MicrobiologyMarch 1, 2026Irene Esteban-Cuesta, Laura Führer, Steffen Porwollik, Weiping Chu, Steven R. Fiddaman, Irmak Sah, Michael McClelland, Claudia Guldimannreaction was subsequently held at 20 °C. The Q5™ Hot Start High-Fidelity 2x Master Mix (Item number M0494L; New England Biolabs, Frankfurt am Main, Germany) was added at a final concentration of 1x. Equal volumes

(16) Integrating natural and engineered genetic variations to decode regulatory influence on blood traitsCell ReportsFebruary 24, 2026Manuel Tardaguila, Dominique Von Schiller, Michela Colombo, Ilaria Gori, Eve L. Coomber, Thomas Vanderstichele, Paola Benaglio, Chiara Chiereghin, Sebastian Gerety, Dragana Vuckovic, et al.PowerUP SYBR green master mix,Applied Biosystems,Cat#A25742 Q5 Hot Start polymerase,New England Biolabs,Cat#M0494S KAPA library quantification kit,Kapa Biosystems,Cat#KK4834 SF Cell Line 4D-Nucleofector™ X Kit,Lonza,Cat#V4XC-2032 P3

(17) Enhanced editing of Bifidobacterium lactis using the endogenous Type I-G CRISPR-Cas systemApplied and Environmental MicrobiologyFebruary 18, 2026Ourania Raftopoulou, Kendall Malmstrom, Meichen Pan, Rodolphe Barrangoucloning were carried out with Q5 Hot Start High-Fidelity 2X Master Mix (NEB, Ipswich, MA, USA; Cat# M0494L), whereas screening of transformants used OneTaq DNA Polymerase (NEB, Ipswich, MA, USA; Cat# M0509L).

(18) Charting the multilevel molecular response to palbociclib in ER-positive breast cancerNAR CancerFebruary 17, 2026Archishma Kavalipati, Amy Aponte, Michael E Sullivan, Sarah L Whittington, José C Martínez, Grant A Goda, Maria M Aleman, Michael J Emanuele, Daniel Dominguezfor T-47D cells was performed using Q5® Hot Start High-Fidelity 2× Master Mix (New England Biolabs, M0494S). Samples were run on 3% agarose (Invitrogen, 16500-500) Tris-acetate-EDTA (TAE) gel (Fisher Scientific,

(19) Chemical inhibition of a bacterial immune systemCell Host & MicrobeFebruary 11, 2026Zhiyu Zang, Olivia K. Duncan, Dziugas Sabonis, Iana Fedorova, Yun Shi, Gause Miraj, Shuai Le, Jun Deng, Yuhao Zhu, Yanyao Cai, et al.NEB® 10-beta Electrocompetent E. coli,New England Biolab,Cat#C3020K Q5® Hot Start High-Fidelity 2X Master Mix,New England Biolab,Cat#M0494 Lysing Matrix B,MP Biomedicals,Cat#116911050 Amicon Ultra-0.5 Centrifugal Filter Units 3 kDa,EMD Millipore,Cat#UFC500396 MagStrep®

(20) Modular gene tagging in C. elegansResearch SquareFebruary 10, 2026Erik Jorgensen, Kevin Hefel, Kevin Kruse, Soren B. Jorgensen, Kam Hoe Jorgensen, Ryan Stolley, Matthew S. Stolley, Matthew Jorgensen(NEB 5-alpha). Amplicons used in cloning were generated with 2X Q5® Hot-Start Master Mix from NEB (#M0494). Manufacturer’s recommendations for extension time, amount of template DNA, and annealing temperature

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(1)

10/6/2023Convenient PCR Master Mix From New England Biolabs Anh NguyenI used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)

3/31/2023Good PCR Master Mix YONG MTry this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)

1/2/2020DNA Insert Amplification for CloningDr. Thippeswamy SannasiddappaHighly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.

Q5 Hot Start High-Fidelity 2X Master Mix

Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

New England Biolabs

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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

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