Q5 Hot Start High-Fidelity 2X Master Mix M0494S from New England Biolabs

Product Specs
ItemQ5 Hot Start High-Fidelity 2X Master Mix
CompanyNew England Biolabs
Price $233.00 Inquire
Catalog NumberM0494S
Size100 reactions
ApplicationsDNA Analysis
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Generative modeling for RNA splicing prediction and designElifeMay 29, 2026Di Wu, Natalie Maus, Anupama Jha, Kevin Yang, Benjamin D Wales-McGrath, San Jewell, Anna Tangiyan, Peter Choi, Jake R Gardner, Yoseph Barashand PCR for the splicing event was performed with the Q5 Hot Start High-Fidelity 2 X Master Mix (NEB, M0494S). PCR reactions were then visualized on a 2% agarose gel in 1 X lithium boric acid buffer (Faster Better Media

(2) Modulation of WNT and FGF18 enhances yield and subtype identity of hPSC-derived midbrain dopamine neuronsJ Clin InvestMay 15, 2026Tae Wan Kim, Jinghua Piao, Vittoria D. Bocchi, So Yeon Koo, Se Joon Choi, Fayzan Chaudhry, Donghe Yang, Hyein S. Cho, Emiliano Hergenreder, Lucia Ruiz Perera, et al.targeting constructs were generated from a genomic DNA PCR with Q5 polymerase (New England Biolabs #M0494) amplifying ~500bp of homology per side and assembled with NEBuilder (New England Biolabs #E2621S).

(3) The Roles of MKI67, GNL2, and MDN1 in Ribosome Biogenesis and Transcriptome Regulation in the Neuronal Cell Line HEK293TbioRxivMay 5, 2026Shiro Iuchi, Joao A. Paulothe cDNA encoding GNL2 (Q13823) was amplified using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0494S). This product was then cloned into the ECORI-SCAII site of pEGFP-C2, yielding pGNL2-G7, which encodes

(4) Reciprocal repulsions enforce heterotypic dendrite segregation in an olfactory circuitbioRxivMay 4, 2026Hui Ji, Jingxian Li, Yizhen Xu, Kenneth Kin Lam Wong, Yunming Wu, David J. Luginbuhl, Yanbo Zhang, Zhuoran Li, Jaeyoon Lee, Robert C Jones, et al.assays,Critical commercial assays Q5 hot-start high-fidelity DNA polymerase,New England Biolabs,Cat# M0494S NEBuilder HiFi DNA assembly master mix,New England Biolabs,Cat# E2621L Deposited data,Deposited data,Deposited

(5) A fast-to-faithful transition shapes the DNA repair landscape during embryogenesisbioRxivMay 1, 2026Shengzhou Wang, Ian Pantziris, Mengsheng Zhang, Yang Liu, James A. Gagnonsamples were amplified with PCR using Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs M0494). PCR amplification as performed with the following conditions: 98 °C for 30 seconds, 25 cycles (tyr),

(6) An anaerobic pathogen rewires host metabolism to fuel oxidative growth in the inflamed gutCellApril 30, 2026Luisella Spiga, Ryan T. Fansler, Yifan Wu, Alexandra Grote, Madison Langford-Butler, Asia K. Miller, Maxwell Neal, Owen F. Hale, Deepanshu Singla, M. Wade Calcutt, et al.Kit,Qiagen,47014 PowerUp SYBR Green Master Mix,Applied Biosystems,A25742 Q5 Hot Start 2x Master Mix,NEB,M0494L Quick-DNA Fungal/Bacterial Miniprep Kit,Zymo Research,D6005 RNAlater™ Stabilization Solution,Invitrogen,AM7020 RNeasy

(7) THE FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycleeLifeApril 30, 2026Taylar Hammond, Jong Bin Choi, Miles W Membreño, Janos Demeter, Roy Ng, Debadrita Bhattacharya, Thuyen N Nguyen, Griffin G Hartmann, Caterina I Colón, Carine Bossard, et al.for FAM53C was amplified by PCR using Q5 Hot Start High-Fidelity 2 X Master Mix (New England Biolabs, M0494S). To select the FAM53C knockout clone, we used the following primer pair: forward, 5′-GGCCCAAGATTCCTCTCGAC-3′,

(8) The FAM53C/DYRK1A axis regulates the G1/S transition of the cell cycleElifeApril 30, 2026Taylar Hammond, Jong Bin Choi, Miles W Membreño, Janos Demeter, Roy Ng, Debadrita Bhattacharya, Thuyen N Nguyen, Griffin G Hartmann, Caterina I Colon, Carine Bossard, et al.for FAM53C was amplified by PCR using Q5 Hot Start High-Fidelity 2 X Master Mix (New England Biolabs, M0494S). To select the FAM53C knockout clone, we used the following primer pair: forward, 5′-GGCCCAAGATTCCTCTCGAC-3′,

(9) Nitrogen metabolism profiling reveals cell state-specific pyrimidine synthesis pathway choiceNature MetabolismApril 29, 2026Milan R. Savani, Bingbing Li, Bailey C. Smith, Wen Gu, Yi Xiao, Gerard Baquer, Tracey Shipman, Skyler S. Oken, Namya Manoj, Lauren G. Zacharias, et al.was amplified by PCR with Q5 Hot Start High-Fidelity 2× Master Mix polymerase (New England Biolabs, M0494). A cDNA library prepared from NHA donor #1 cells with the RNeasy Mini kit (QIAGEN, 74004) and LunaScript RT Supermix

(10) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marrowElifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livak, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,Propidium iodide,Sigma-Aldrich,P4170, "Chemical compound, drug",Q5 Hot Start High-Fidelity 2X Master Mix,NEB,M0494S, "Chemical compound, drug",Richard-Allan Scientific Cover glass,Epredia,102424, "Chemical compound,

(11) Single-cell lineage tracing identifies hemogenic endothelial cells in the adult mouse bone marroweLifeApril 27, 2026Jing-Xin Feng, Mei-Ting Yang, Lili Li, Caiyi C Li, Ferenc Livák, Jack Chen, Yongmei Zhao, Dunrui Wang, Avinash Bhandoola, Naomi Taylor, et al.,Propidium iodide,Sigma-Aldrich,P4170, "Chemical compound, drug",Q5 Hot Start High-Fidelity 2X Master Mix,NEB,M0494S, "Chemical compound, drug",Richard-Allan Scientific Cover glass,Epredia,102424, "Chemical compound,

(12) Ribosome Molecular Aging Shapes Translation DynamicsbioRxivApril 25, 2026Jordy F. Botello, Lifei Jiang, Peter J. Metzger, Troy J. Comi, Aya A. Abu-Alfa, Qiwei Yu, Margaret S. Ebert, Minkyu Lee, Lennard W. Wiesner, Maya Butani, et al.DNA fragments were amplified from original plasmids by PCR using Q5 High-Fidelity 2X Master Mix (NEB, M0494L) with oligonucleotides synthesized by IDT, then the In-Fusion HD Cloning kit (Takara, 638949) was used

(13) Editing stem cell genomes at scale to measure variant effects in diverse cell and genetic contextsmedRxivApril 24, 2026Shawn Fayer, Riddhiman K. Garge, Melissa Hopkins, Clayton E. Friedman, Abby V. McGee, Joshua Rico, Rachel L. Powell, Evan McDermot, Nahum T. Smith, Sriram Pendyala, et al.MYBPC3 allele specific PCR was conducted with Q5 Hot Start High-Fidelity 2x Master Mix (NEB; cat. no. M0494S). POLG allele specific PCR was performed with LongAmp Taq DNA Polymerase (NEB; cat. no.M0323S). gDNA

(14) Disruption of Cell-Type-Specific Molecular Programs of Medium Spiny Neurons in AutismbioRxivApril 23, 2026Guohua Yuan, Varun Suresh, Emilie Wigdor, Yuhan Hao, Rachel Leonard, Marilyn Steyert, Michael Griffiths, Clements Evans, Narjes Rohani, Jakob Weiss, et al.were subamplified from each 10X cDNA library using the Q5 Hot Start High Fidelity 2× master mix (NEB, M0494). In brief, 10 μl of cDNA was used as template in a 50 μl PCR reaction containing STICR barcode read

(15) Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunityNat CommunApril 20, 2026Kotaro Chihara, Aa Haeruman Azam, Artyom A. Egorov, Ilya Terenin, Masanori Hashino, Koichi Watashi, Kazuhiro Horiba, Vasili Hauryliuk, Kotaro Kigaresuspended in 10 mM Tris-HCl (pH 8.5). Bead-bound DNA was amplified using Q5 Hot-Start Master Mix (NEB, #M0494S) and Recovery PCR primers in 50 µL reactions. Following thermal cycling, supernatants were recovered

(16) CHARIOT-AAV: Conjugation of diverse vectors to adeno-associated viruses for delivery of large genesbioRxivApril 18, 2026Keisuke Nagao, Tatsuya Osaki, Sho Toyonaga, Chae Gyu Lee, Emmanuel Vargas Vargas Paniagua, Emily Crespin Crespin Guerra, Scott Machen, Jacob L. Beckham, Mriganka Sur, Daniel Griffith Anderson, et al.from the split SpCas9 (Rma) constructs by PCR using Q5 Hot Start High-Fidelity 2X Master Mix (NEB #M0494S). dCas9-KRAB-MeCP2 was also split at several sites (L833/S834, Q844/S845, D850/S851, R859/S860, K867/S868,

(17) Force-modulated structural landscape of the catch bonding F-actin crosslinker α-actinin-4bioRxivApril 16, 2026Alfred C. Chin, Fatemah Mukadum, Matthew J. Reynolds, Glen M. Hocky, Gregory M. AlushinKOD Hot Start Master Mix (MilliporeSigma, 71842) or Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs, M0494L), and PCR products were purified using a QIAquick Gel Extraction Kit (Qiagen, 28704). Plasmid assembly

(18) Rapid and precise amplicon-based genome sequencing to preparedness and response chikungunya virus outbreaksEmerg Microbes InfectApril 15, 2026Kaiying Wang, Jinhui Li, Tianyuan Liu, Peihan Li, Ning Wang, Yuqi Liu, Yufeng Liang, Fenxiang Li, Chuhong Wei, Huacheng Yan, et al.by multiple amplification. The amplification system included Q5® High-Fidelity 2X Master Mix (NEB #M0494) 6.25 µL, cDNA 4.5 µL and primer pool 1/2 1.75 µL. The pool 1 and pool 2 need to be amplified separately.

(19) 5′ UTR length shapes alternative N-terminal protein isoforms across cancers and in rare diseaseEMBO ReportsApril 13, 2026Jimmy Ly, Eric M Smith, Matteo Di Bernardo, Yi Fei Tao, Elizabeth M Black, Ekaterina Khalizeva, Iain M Cheeseman5 min. The resulting cDNA was amplified by PCR using Q5 Hot Start High-Fidelity 2× Master Mix (NEB, M0494), with 5% of the reverse-transcription product used per reaction. To enhance PCR specificity, nested

(20) Direct RNA nanopore sequencing reveals rapid RNA modification changes following glucose stimulation of human pancreatic beta-cell linesbioRxivApril 12, 2026Logan Mulroney, Henry J. Taylor, Angela Lee, Amy J. Swift, Mihail Zdravkov, Lori L. Bonnycastle, Shelise Y. Brooks, Brian N. Lee, Tomas Fitzgerald, Narisu Narisu, et al.cDNA products for 15 cycles at 98°C for 10 seconds, 65°C for 15 seconds, and 72°C for 3 minutes (NEB M0494). We purified the amplicons with AMPure XP Beads (Beckman Coulter™) according to the manufacturer’s

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(1)

10/6/2023Convenient PCR Master Mix From New England Biolabs Anh NguyenI used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)

3/31/2023Good PCR Master Mix YONG MTry this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)

1/2/2020DNA Insert Amplification for CloningDr. Thippeswamy SannasiddappaHighly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.

Q5 Hot Start High-Fidelity 2X Master Mix

Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

New England Biolabs

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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

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