Q5 Hot Start High-Fidelity 2X Master Mix M0494S from New England Biolabs

Product Specs
ItemQ5 Hot Start High-Fidelity 2X Master Mix
CompanyNew England Biolabs
Price $233.00 Inquire
Catalog NumberM0494S
Size100 reactions
ApplicationsDNA Analysis
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Virulence attenuation in ST11-K64 Klebsiella pneumoniae explains its divergent clinical manifestation from ST23-K1VirulenceDecember 31, 2026Shujing Li, Ying Zhang, Qian Zeng, Lilan Zheng, Jing Yan, Yong Wang, Yun Liu, Lianhua Yu, Yi Li, Meng Li, et al.pACYC-iucA and pACYC-rmpA2 were constructed using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, M0494), following the manufacturer’s recommended protocol. Additionally, the pACYC184 plasmid was electrotransferred

(2) Site-specific inhibition of translation initiation via 2'-O-methylationNucleic Acids ResSeptember 28, 2026Adam Suh, Stephanie Mou, Emmely A Patrasso, Hannah Serio, Rui Wang, Yue Zhao, Kevin Vasquez, Smriti Sangwan, Yang Yi, Daniel ArangoPCR was performed using the Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs, Cat. #: M0494). All leader sequences were in vitro transcribed using the HiScribe® T7 High Yield RNA Synthesis Kit

(3) Baseline cellular state shapes the molecular impact of mutant Kirsten rat sarcoma viral oncogene homologue (KRAS) alleles in reconstituted pancreatic cancer cellsMol OmicsSeptember 21, 2026Yanixa Quiñones-Avilés, Barbora Salovska, Cassandra S Markham, Yi Di, Benjamin E Turk, Yansheng Liu, Mandar Deepak MuzumdarMutant Clone Collection (Addgene, Kit #1000000089) with Q5 Hot Start High-Fidelity DNA Polymerase (NEB, M0494S) using forward primers containing a NheI-HF (NEB, R3131S) or BamHI-HF (NEB, R3136S) restriction digest

(4) Protocol for robust gene knockout and reliable validation in human cell lines using quad-guide RNA vectorsSTAR ProtocSeptember 16, 2026Michael Tobin, Jie Dai, Li-Chuan Chan, Weiwei GuoPES,Fisherbrand,Cat# 09–720–514 Q5 Hot Start High Fidelity 2× Master Mix,New England Biolabs,Cat#: M0494S Nanodrop One Spectrophotometer,Thermo Scientific,Cat#: ND-ONE-C SimpliAmp Thermal Cycler,Applied Biosystems,Cat#:

(5) Jamestown Canyon Virus Rapidly Adapts to Mosquito Cells Through Multiple M Segment MutationsVirusesSeptember 15, 2026Sarah Dysinger, Tamanna Srivastava, Sara Cherry, Paul Batesreading frame were amplified using Q5 High-Fidelity 2X Hot Start Master Mix (New England Biolabs, Cat #M0494S, Ipswich, MA, USA) and the primers listed in Supplemental Table S4. Primer overhangs were designed to

(6) Hepatitis B virus genome mutations in precore and basal core promoter regions among HBeAg-negative chronic hepatitis B patients with high viral load in IndonesiaPLoS OneSeptember 15, 2026Dewi Wulandari, Yan Mardian, Sunita, Kadek Aditya Darmayoga, Rindha Agustiani, Khoirunnisa, Youdiil Ophinni[22]. Amplification was performed using Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs, M0494) and Biorad CFX96 TM Real Time PCR system. PCR amplification consisted of an initial denaturation at

(7) Gliomas phenocopy an inborn error of metabolism to drive neuronal activity and tumor growthCellSeptember 15, 2026Kalil G. Abdullah, Kenji Miki, Charles K. Edgar, Shuangcheng Alivia Wu, Yi Xiao, Yuan-Tai Huang, Richard Drexler, Saritha Krishna, Vinesh T. Puliyappadamba, Li Wei Rachel Tay, et al.Kit,Miltenyi Biotec,Cat# 130-095-942 Q5 Hot Start High-Fidelity 2X Master Mix,New England Biolabs,Cat# M0494 QIAquick Gel Extraction Kit,QIAGEN,Cat# 28706 Monarch PCR & DNA Cleanup Kit,New England Biolabs,Cat#

(8) Cell cycle regulation of polymerase theta mediated end-joining underlies its sensitivity to loss of BLM-DNA2bioRxivSeptember 14, 2026Connor P. McBrine, Ryan B. Jensen, Megan C. KinggDNA, 0.5 μM forward primer, 0.5 μM reverse primer, and Q5 Hot Start High-Fidelity Master Mix at 1X (M0494S, NEB). Cycling conditions were: initial denaturation at 98°C for 30 s, then (98°C for 10 s, 66°C for

(9) A PDGFRB-CD44-MIF crosstalk promotes cancer-associated cellular phenotypes in ATRT-TYR/MYC cell linesFront OncolSeptember 9, 2026Yacine A. Choutri, Liming Xu, Fupan Yao, Annie HuangCat#GB300). The regions flanking gRNA target sites were amplified by PCR (New England Biolabs, Cat#M0494), using primers listed in Supplementary Table 6. Primers were designed using the Primer Wizard tool

(10) Systemically delivered mRNA-LNPs transfect primary and secondary liver tumorsMolecular Therapy Nucleic AcidsSeptember 8, 2026Laura J. Leighton, Yee Jing Gee, Sachithrani U. Madugalle, Maria Victorova, Nissa L. Carrodus, Kim R. Bridle, Sidney A. Howell, Xiaowen Liang, Gregory C. Miller, Chris L.D. McMillan, et al.(Integrated DNA Technologies, Singapore). DNA was amplified using 2X Q5 PCR master mix (New England Biolabs M0494L) with a forward primer containing a T7 promoter and a reverse primer encoding a 126-nt poly(A) tail.

(11) UV irradiation drives lineage-specific MITF-mediated transcription of PD-L1 to confer immune tolerance to UV-mutated melanocytesImmunitySeptember 8, 2026Jennifer A. Lo, Inbal Rachmin, Jessica L. Flesher, Xunwei Wu, Akinori Kawakami, Miroslav Hejna, Judith R. Boozer, Nhu Nguyen, Andrew D. King, Yawen Ji, et al.peptide (KVPRNQDWL),GenScript,Cat# RP20344 Q5 high-fidelity DNA polymerase,New England Biolabs,"Cat# M0494S, M0494L" TRIzol reagent,Invitrogen / Life Technologies,Cat# 15596018 DAPI,Thermo Fisher Scientific,Cat#

(12) Establishment of a Common Marmoset Lineage Carrying a Frameshift Mutation in SETD1A, a Schizophrenia Risk GeneJ NeurochemSeptember 3, 2026Zefeng Wei, Harumi Nakao, Yuichi Hiraoka, Maria Harbers, Yukiko Abe, Motoki Goto, Moe Tamano, Mai Saeki, Yusuke Kishi, Atsu AibaLibrary amplification was performed using Q5 Hot Start High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0494S) under the following thermal cycling conditions: 5 min at 72°C and 30 s at 98°C; followed by 12 cycles

(13) Integrated surveillance in Kilifi reveals continued SARS-CoV-2 circulation and immune escape of emerging JN.1 sublineages post-pandemicFront Cell Infect MicrobiolSeptember 3, 2026Gloria Konyino, Bernadette Kutima, Sharon Owuor, Donwilliams Omuoyo, Gerald Waweru, Igor Andrade Santos, Arnold W. Lambisia, Esther Nyadzua Katama, Angela Maina, Joyce Uche Nyiro, et al.Then, 1.3 µL of cDNA, 2 μL of primer pools, 6.3 μL of Q5 Hot Start High-Fidelity 2X Master Mix (NEB M0494, MA, USA), and 1.9 μL of nuclease-free water were combined in the reaction under the following thermocycling

(14) A tailored in vivo CRISPR screen identifies BAP1 as a potent tumor suppressor of sarcomaJCI InsightAugust 24, 2026Jianguo Huang, Xingliang Liu, Warren Floyd, William Haugh, Zhaoyu Sun, Melissa J. Kasiewicz, Yaping Wu, Brian Piening, John T. Welle, Wesley K. Rosales, et al.performed with specific primer (Supplemental Table 4) using Q5 HiFi DNA polymerase (New England Biolabs, M0494) (11). PCR was sent to Azenta for Sanger sequencing or next-generation sequencing. Western blot analysis. NIH

(15) Efficient and Fast Site-Directed Mutagenesis via Partially or Completely Overlapping Primer PairsBio ProtocAugust 20, 2026Paulina Varela-Castillo, Arezousadat Razavi, Changsheng Zhao, Martin M. Geng, Amitis Nour, Xiang-Jiao Yangcatalog number: 12368010) or Q5® High-Fidelity DNA Polymerase (New England Biolabs, catalog number: M0494S) Note: Notably, while we have used these two enzymes extensively, we have not tested their related preparations,

(16) FHL3 Regulates Vascular Smooth Muscle Cell Phenotypic Switching Through the MRTFB-SRF Signaling AxisCellsAugust 17, 2026Xiaoxin Huang, Heming Zhang, Yanhong Zhang, Charles U. Solomon, David G. McVey, Shu Yegenomic DNA using Q5 Hot Start High-Fidelity 2× Master Mix (New England Biolabs, Ipswich, MA, USA; M0494L). Polymerase chain reaction (PCR) primers were designed according to the protocol reported by Joung

(17) Profiling Protein‐Peptide Interactions by Yeast Surface DisplayCurr ProtocAugust 8, 2026Joseph D. Hurley, Andrew C. Kruseplates containing ampicillinQ5 Hot Start High‐Fidelity 2× Master Mix (New England Biolabs, cat. no. M0494S) or equivalent high‐fidelity PCR master mixPCR primers flanking peptide region (e.g., Insert F1 and

(18) CLOCK/BMAL1 interactome uncovers homeodomain factors as tissue regulatorsNature Cell BiologyAugust 6, 2026Fatih Aygenli, Lukas A. Huschet, Tanja Popp, Andrea Ribeiro, Darina Barkhatova, Céline Jouffe, Riccardo Trozzo, Jerome S. Menet, Roland Rad, Kenneth A. Dyar, et al.Myc_Kpni_For and Myc_Sali_Rev primers using Q5 Hot Start High-Fidelity 2X Master Mix (Q5 Mix, NEB, M0494) and inserting it into the Kpni and Sali sites of the pCMV-SPORT6-Prox1 plasmid (N terminal of Prox1).

(19) A self-complementary recombinant adeno-associated virus vector coding for an anchorless prion protein carrying the G127V mutation extends survival in a rodent prion disease modelPLoS PathogAugust 4, 2026Thomas Zerbes, Claire Verkuyl, Cunjie Zhang, Sophie Grunnesjoe, Shehab Eid, Hamza Arshad, Wenda Zhao, Zahra Nasser, Teaghan O'Shea, Ari Bel, et al.reaction, 50 ng of gDNA was amplified with Q5 Hot Start High-Fidelity 2X Master Mix (catalog number M0494S, New England Biolabs) and forward and reverse primers (final concentration 500 nM) that map to gDNA

(20) BASELINE: a CRISPR base editing platform for mammalian-scale single-cell lineage tracingNucleic Acids ResAugust 4, 2026Evan Winter, Francesco Emiliani, Aidan Cook, Asma Abderrahim, Aaron McKennaamplification reactions were performed using Q5 Hot Start 2× Master Mix (New England Biolabs, Cat. No. M0494L) with SYBR™ Green I Nucleic Acid Gel Stain (Thermo Fisher Scientific, Cat. No. S7563) on a CFX Connect

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(1)

10/6/2023Convenient PCR Master Mix From New England Biolabs Anh NguyenI used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)

3/31/2023Good PCR Master Mix YONG MTry this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)

1/2/2020DNA Insert Amplification for CloningDr. Thippeswamy SannasiddappaHighly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.

Q5 Hot Start High-Fidelity 2X Master Mix

Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

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