Bst2.0 DNA Polymerase M0537L from New England Biolabs

Product Specs
ItemBst2.0 DNA Polymerase
CompanyNew England Biolabs
Price $311.00 Inquire
Catalog NumberM0537L
Size8000 units
ApplicationsDNA Analysis
TypeDNA Polymerase
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) sc-rDSeq: a robust and cost-effective full-length total RNA sequencing method for single cells reveals multilayered heterogeneity in drug-resistant lung cancer cellsNucleic Acids ResApril 13, 2026Xue Sun, Shir-Liya Dadon, Dena Ennis, Wenpeng Fan, Muhammad Awawdy, Eli Reuveni, Adi Alajem, Oren Ramand hybridization at 60°C for 20 min, 5 μl of primer extension mix containing 1.8 U of DNA Polymerase (NEB, cat #M0537) and 0.3 mM dNTP (NEB, cat #N0446) in 1× isothermal amplification buffer (NEB, cat #B0537) was added,

(2) Subsistence transition preceded population turnover in the eastern Colombian AndesbioRxivMarch 27, 2026Kendra Sirak, Miguel Delgado, Angélica Triana, Sebastián Rivas, Pedro Argüello, Ana María Boada, Javier Rivera-Sandoval, German Peña, Carl Langebaek, Juan Pablo Ospina, et al.Ligase Buffer,New England Biolabs,B0216L "Bst DNA Polymerase2.0, large frag.",New England Biolabs,M0537 UGI,New England Biolabs,M0281 USER enzyme,New England Biolabs,M5505 Buffer PB,QIAGEN,19066 Buffer

(3) Papuan admixture predated the settlement of PalauCellMarch 10, 2026Yue-Chen Liu, Joanne Eakin, Jolie Liston, Rosalind Hunter-Anderson, Calvin Emesiochel, Kiblas Soaladaob, Sunny O. Ngirmang, Olivia Cheronet, Carla S. Hadden, Alexander Cherkinsky, et al.Ligase Buffer,New England Biolabs,B0216L "Bst DNA Polymerase2.0, large frag.",New England Biolabs,M0537 UGI,New England Biolabs,M0281 USER enzyme,New England Biolabs,M5505 Buffer PB,QIAGEN,19066 Buffer

(4) Population genetic diversity of invasive Pomacea snails and surveillance of Angiostrongylus cantonensis in Shanghai, East ChinaParasites & VectorsMarch 9, 2026Peter S. Andrus, Qing-chi Han, Li-min Yang, Christopher M. Wade, Zhi-qiang Qin, Kokouvi Kassegne, Xiao-nen Wu, Yun-hai Guo, Xiao-nong ZhouThe Anc-COI LAMP reaction was conducted in a total volume of 25 µL using New England Biolabs Bst 2.0 (M0537L). The reaction mixture contained 12.5 µL of buffer mix (0.8 mM MgSO4, 1.4 mM dNTPs, and 0.8 M betaine),

(5) LAMP-coupled CRISPR-Cas12a assays: A promising new tool for molecular diagnosis of leishmaniasisPLOS Neglected Tropical DiseasesFebruary 13, 2026Eva Dueñas, Ingrid Tirado, Percy Huaihua, Ariana Parra Parra del Riego, Luis Cabrera-Sosa, Jose A. Nakamoto, María Cruz, Carlos M. Restrepo, Jorge Arévalo, Vanessa Adauiconditions using Bst 2.0 DNA polymerase (New England Biolabs Inc. (NEB), Massachusetts, USA; Cat no. M0537) according to the manufacturer’s protocol. The only modification was the final total concentration of

(6) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruziMicrobiology SpectrumNovember 4, 2025Sneider Alexander Gutierrez Guarnizo, Beth Jessy Condori, Luciana Basma, Shirley Equilia, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.Isothermal Amplification Buffer (20 mM Tris-HCl pH 8.8, NEB, CN: B0537S), and 8 U Bst Polymerase 2.0 WarmStart (NEB, CN: M0537L). Two microliters of DNA template were added to the master mix to achieve a total volume of 25 µL. SYBR

(7) Long shared haplotypes identify the southern Urals as a primary source for the 10th-century HungariansCellOctober 16, 2025Balázs Gyuris, Leonid Vyazov, Attila Türk, Pavel Flegontov, Bea Szeifert, Péter Langó, Balázs Gusztáv Mende, Veronika Csáky, Andrey A. Chizhevskiy, Ilgizar R. Gazimzyanov, et al.Ligase Buffer,New England Biolabs,B0216L "Bst DNA Polymerase2.0, large frag.",New England Biolabs,M0537 UGI,New England Biolabs,M0281 USER enzyme,New England Biolabs,M5505 Buffer PB,QIAGEN,19066 Buffer

(8) qByte: An open-source isothermal fluorimeter for democratizing analysis of nucleic acids, proteins and cellsPLOS BiologyMay 28, 2025Francisco J. Quero, Guy Aidelberg, Hortense Vielfaure, Yann Huon Huon de Kermadec, Severine Cazaux, Amir Pandi, Ana Pascual-Garrigos, Anibal Arce, Samuel Sakyi, Urs Gaudenz, et al.SYTO9 fluorescent dye (S34854, Thermo Fisher Scientific), and 0.32 U/μL of Bst 2.0 DNA polymerase (M0537L, New England Biolabs). For SARS-CoV-2 detection, an additional 0.32 U/μL of RTx retrotranscriptase (M0380L,

(9) The histone chaperone Spn1 preserves chromatin protections at promoters and nucleosome positioning in open reading framesG3: Genes, Genomes, GeneticsApril 17, 2025Andrew J Tonsager, Alexis Zukowski, Catherine A Radebaugh, Abigail Weirich, Laurie A Stargell, Srinivas RamachandranBeads were washed, and second-strand synthesis was performed using Bst 2.0 DNA polymerase (NEB cat. M0537) with an increasing temperature gradient of 15–31°C with shaking at 1,750 rpm. Beads were washed, and

(10) Scalable acoustic virtual stirrer for enhanced interfacial enzymatic nucleic acid reactionsSci AdvMarch 7, 2025Dayang Li, Kunjie Li, Jianquan Li, Dongfang Li, Heng Chen, Sen-Sen Li, Chaoyong Yang, Huimin Zhang, Lu-Jian Chen, Xuejia Huinput strands. The 10-μl input mix for both OR and AND gates contained Bst DNA Polymerase (0.4 U/μl M0537, NEB), 20 mM tris-HCl, 5 mM dNTP mix, 2 mM MgSO4, 50 mM KCl, 10 mM (NH4)2SO4, 0.1% (v/v) Tween 20, and

(11) Multiplexed detection of respiratory virus RNA using optical pH sensors and injection-molded centrifugal microfluidicsMikrochim ActaFebruary 12, 2025Gianmarco Domenico Suarez, Yuki Yu Kiu Tang, Steevanson Bayer, Peter Pak-Hang Cheung, Stefan Naglunits µL−1 RTx reverse transcriptase (M0380L, NEB, USA), and 0.32 units/µL Bst 2.0 DNA polymerase (M0537M, NEB, USA). Fifteen-microliter reaction volumes were incubated at 55 °C for 15 min and 65 °C for 35

(12) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruzimedRxivJanuary 13, 2025Sneider Alexander Gutierrez Guarnizo, Luciana Basma, Shirley Equilia, Beth Jessy Condori, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.Isothermal Amplification Buffer (20 mM Tris-HCl pH 8.8, NEB, CN: B0537S), and 8 U Bst Polymerase 2.0 WarmStart (NEB, CN: M0537L). Two microliters of DNA template were added to the master mix to achieve a total volume of 25 μL. SYBR

(13) A drop dispenser for simplifying on-farm detection of foodborne pathogensPLOS ONEDecember 31, 2024Mohsen Ranjbaran, Simerdeep Kaur, Jiangshan Wang, Bibek Raut, Mohit S. Verma(1 U/μL; New England Biolabs, M0372S), 5.4 μL Bst2.0 DNA Polymerase (120 U/μL; New England Biolabs, M0537M), 20 μL phenol red solution (25 mM; Sigma-Aldrich, P3532), and 99.4 μL nuclease-Free water (Fisher Scientific,

(14) Population expansion from central plain to northern coastal China inferred from ancient human genomesiScienceDecember 20, 2024Baitong Wang, Daohua Hao, Yu Xu, Kongyang Zhu, Rui Wang, Xiaomin Yang, Qu Shen, Mengting Xu, Tianyou Bai, Hao Ma, et al.Isothermal amplification buffer,NEB,B0537S Deoxynucleotide (dNTP) solution mix,NEB,N0447L Bst 2.0 DNA polymerase,NEB,M0537L AMPure XP Beads,Beckman,A63881 Agarose,Biowest,111860 Tris-EDTA buffer solution (1003),Sigma,T9285 ,, Critical

(15) Ancient DNA challenges prevailing interpretations of the Pompeii plaster castsCurrent BiologyNovember 18, 2024Elena Pilli, Stefania Vai, Victoria C. Moses, Stefania Morelli, Martina Lari, Alessandra Modi, Maria Angela Diroma, Valeria Amoretti, Gabriel Zuchtriegel, Massimo Osanna, et al.M0201 T4 DNA Polymerase,New England Biolabs,Cat# M0203 Bst DNA Polymerase,New England Biolabs,Cat# M0537L Quick Ligase,New England Biolabs,Cat# M2200 PfuTurbo Cx Hotstart DNA Polymerase,Agilent Technologies,Cat#

(16) Multiomic characterization of RNA microenvironments by oligonucleotide-mediated proximity-interactome mappingNature methodsNovember 1, 2024Tsue AF, Kania EE, Lei DQ, Fields R, McGann CD, Marciniak DM, Hershberg EA, Deng X, Kihiu M, Ong SE, et al.Supplementary Table 1), in 10 mM MgSO4, 0.6 mM each ATP, CTP, and TTP, 4 U Bst 2.0 DNA Polymerase (NEB; M0537), 1x PBS, in a final volume of 50 μL. Reactions were incubated at 37°C for two hours in a thermocycler,

(17) Enhancing homology-directed repair efficiency with HDR-boosting modular ssDNA donorNature CommunicationsAugust 10, 2024Ying-Ying Jin, Peng Zhang, Le-Le Liu, Xiang Zhao, Xiao-Qing Hu, Si-Zhe Liu, Ze-Kun Li, Qian Liu, Jian-Qiao Wang, De-Long Hao, et al.88817), and a 5′-tailed primer was used to copy the template strand with the Bst polymerase 2.0 (NEB, M0537L). After the removal of 3′ overhangs via T4 DNA polymerase (Thermo Fisher Scientific, EP0062), a second

(18) THE HISTONE CHAPERONE SPN1 PRESERVES SUBNUCLEOSOMAL STRUCTURES AT PROMOTERS AND NUCLEOSOME POSITIONING IN OPEN READING FRAMESbioRxivAugust 2, 2024Andrew J. Tonsager, Alexis Zukowski, Catherine A. Radebaugh, Abigail Weirich, Laurie A. Stargell, Srinivas RamachandranBeads were washed, and second-strand synthesis was performed using Bst 2.0 DNA polymerase (NEB cat. M0537) with an increasing temperature gradient of 15-31°C with shaking at 1750 rpm. Beads were washed, and

(19) Simultaneous de novo calling and phasing of genetic variants at chromosome-scale using NanoStrand-seqCell DiscoveryJuly 9, 2024Xiuzhen Bai, Zonggui Chen, Kexuan Chen, Zixin Wu, Rui Wang, Jun’e Liu, Liang Chang, Lu Wen, Fuchou Tangincubated for 5 min at room temperature. Next, 0.5 μL Bst 2.0 DNA Polymerase (New England Biolabs; M0537), 1 μL 10× Isothermal Amplification Buffer, 0.5 μL 10 mM dNTPs, and 0.75 μL H2O were subsequently added

(20) Protocol for a comprehensive pipeline to study ancient human genomesSTAR ProtocolsJune 21, 2024Kongyang Zhu, Haifeng He, Le Tao, Hao Ma, Xiaomin Yang, Rui Wang, Jianxin Guo, Chuan-Chao WangIsothermal amplification buffer,NEB,B0537S Deoxynucleotide (dNTP) solution mix,NEB,N0447L Bst 2.0 DNA polymerase,NEB,M0537L AMPure XP Beads,Beckman,A63881 Agarose,Biowest,111860 Tris-EDTA buffer solution (100×),Sigma,T9285 ,, Critical

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Bst2.0 DNA Polymerase

Bst2.0 DNA Polymerase from New England Biolabs

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Bst2.0 DNA Polymerase from New England Biolabs

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