Ubiquitin Rhodamine 110 C-Terminal Derivative Rhodamine 110-conjugated Protein U-555-050 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemUbiquitin Rhodamine 110 C-Terminal Derivative Rhodamine 110-conjugated Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $451.00Supplier Page View Company Product Page
Catalog NumberU-555-050
Size50 ug
ApplicationsEnzyme Activity
Conjugate/TagRhodamine 110
FormatSupplied as a solution in DMSO
Molecular Weight8.9 kDa
SpeciesHuman
Molecule NameUbiquitin
Source/Expression SystemE. coli-derived Recombinant Human Ubiquitin Rhodamine 110 Protein, CF
NCBI Full Gene Nameribosomal protein S27a, ubiquitin B
NCBI Gene AliaseseS31, UBCEP1, S27A, HEL112, UBCEP80, HEL-S-50, UBC, CEP80, UBA80
StorageProtect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening

Specifications/Features

Available conjugates: Sizes Available: 50 ug

Add to Compare List

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Small-molecule allosteric activator of ubiquitin-specific protease 7 (USP7)Proceedings of the National Academy of SciencesOctober 21, 2025Isabella Jaen Maisonet, Mona Sharafi, Emilie J. Korchak, Andres Salazar-Chaparro, Ariana S. Bratt, Tvesha Parikh, Anthony C. Varca, Binita Shah, Michael Darnowski, Mia Chung, et al.assay buffer (50 mM Tris pH 8.0, 50 mM NaCl, 5 mM TCEP, 0.002% Tween20). UbRho (Bio-Techne catalog # U-555-050) was added to a final concentration of 500 nM to initiate the reaction. The enzyme initial velocity

(2) Discovery of indane and naphthalene derivatives as USP7 inhibitorsEuropean journal of medicinal chemistryOctober 5, 2025Zhang X, Tan S, Wang D, Zhu H, Chen D, Chen Y, Wu G, Zhu Z, Zhang F, Liang Z, et al.

(3) Dynamic networks connect the USP14 active site region with the proteasome interaction surfaceProtein SciMarch 17, 2025Johannes Salomonsson, Linda Sjöstrand, Arvid Eskilson, Dean Derbyshire, Pádraig D'Arcy, Maria Sunnerhagen, Alexandra AhlnerpH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 mg/mL BSA, 1 mM DTT, and 2 mM ATP. 1 μM Ub‐rhodamine (Bio‐Techne, U‐555‐050) was added to start the reaction. The hydrolysis of Ub‐rhodamine was monitored with a Promega plate

(4) Transient interdomain interactions in free USP14 shape its conformational ensembleProtein ScienceMay 1, 2024Johannes Salomonsson, Björn Wallner, Linda Sjöstrand, Pádraig D'Arcy, Maria Sunnerhagen, Alexandra Ahlner50 mM NaCl, 5 mM MgCl2, 1 mg/mL BSA, 1 mM DTT, and 2 mM ATP; 1 μM ubiquitin‐rhodamine (Bio‐Techne, U‐555‐050) was added to start the reaction. The hydrolysis of ubiquitin‐rhodamine was monitored with a Promega

(5) SARS-CoV-2 papain-like protease activates nociceptors to drive sneeze and painbioRxiv : the preprint server for biologyJanuary 11, 2024Mali SS, Silva R, Gong Z, Cronce M, Vo U, Vuong C, Moayedi Y, Cox JS, Bautista DM.

(6) Neuroinflammation inhibition by small-molecule targeting USP7 noncatalytic domain for neurodegenerative disease therapyScience advancesAugust 12, 2022Zhang XW, Feng N, Liu YC, Guo Q, Wang JK, Bai YZ, Ye XM, Yang Z, Yang H, Liu Y, et al.

(7) USP25 inhibition ameliorates Alzheimer's pathology through the regulation of APP processing and Aβ generationThe Journal of clinical investigationMarch 1, 2022Zheng Q, Song B, Li G, Cai F, Wu M, Zhao Y, Jiang L, Guo T, Shen M, Hou H, et al.USP25 catalytic domain (His-USP25aCat, amino acids 157–706) using ubiquitin-rhodamine 110 (R&D System, U-555-050) as a substrate in 384-well white plates (Corning, 3570) as previously described (20). Briefly, different

(8) Rational Development and Characterization of a Ubiquitin Variant with Selectivity for Ubiquitin C-Terminal Hydrolase L3BiomoleculesJanuary 1, 2022Hewitt CS, Das C, Flaherty DP.

(9) Identification of deubiquitinase inhibitors via high-throughput screening using a fluorogenic ubiquitin-rhodamine assaySTAR ProtocolsDecember 17, 2021Anthony C. Varca, Dominick Casalena, Douglas Auld, Sara J. Buhrlage"Kanamycin sulfate, ultrapure",Thermo Scientific,Cat# J1792414 Ubiquitin-Rhodamine 110 (Ub-Rho),R&D Systems,Cat#U-555-050 ,, Deposited data,Deposited data,Deposited data ,, Primary and dose response confirmation screening

(10) Investigation of USP30 inhibition to enhance Parkin-mediated mitophagy: tools and approachesThe Biochemical journalDecember 10, 2021Tsefou E, Walker AS, Clark EH, Hicks AR, Luft C, Takeda K, Watanabe T, Ramazio B, Staddon JM, Briston T, et al.

(11) Identification and validation of selective deubiquitinase inhibitorsCell Chemical BiologyDecember 1, 2021Anthony C. Varca, Dominick Casalena, Wai Cheung Chan, Bin Hu, Robert S. Magin, Rebekka M. Roberts, Xiaoxi Liu, He Zhu, Hyuk-Soo Seo, Sirano Dhe-Paganon, et al.CID: 4383255 ,, ,, ,, ,, ,, ,, ,, ,, ,, Ubiquitin-Rhodamine 110 (Ub-Rho),R&D Systems,Cat#U-555–050 Ubiquitin-propargylamine (Ub-PA),UbiQ,Cat#UbiQ-076 Ubiquitin-vinyl methyl ester (Ub-VME),UbiQ,Cat#UbiQ-054 Critical

(12) Cytotoxic unsaturated electrophilic compounds commonly target the ubiquitin proteasome systemScientific reportsJuly 8, 2019Selvaraju K, Mofers A, Pellegrini P, Salomonsson J, Ahlner A, Morad V, Hillert EK, Espinosa B, Arnér ESJ, Jensen L, et al.

(13) Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15Structure (London, England : 1993)April 2, 2019Teyra J, Singer AU, Schmitges FW, Jaynes P, Kit Leng Lui S, Polyak MJ, Fodil N, Krieger JR, Tong J, Schwerdtfeger C, et al.

(14) Hypoxia-induced 26S proteasome dysfunction increases immunogenicity of mesenchymal stem cellsCell death & diseaseJanuary 28, 2019Abu-El-Rub E, Sequiera GL, Sareen N, Yan W, Moudgil M, Sabbir MG, Dhingra S.

(15) ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome StabilityMolecular cellApril 5, 2018Haahr P, Borgermann N, Guo X, Typas D, Achuthankutty D, Hoffmann S, Shearer R, Sixma TK, Mailand N.

(16) Reactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymesNature communicationsMarch 21, 2018Hewings DS, Heideker J, Ma TP, AhYoung AP, El Oualid F, Amore A, Costakes GT, Kirchhofer D, Brasher B, Pillow T, et al.

(17) Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10Journal of molecular biologyNovember 10, 2017Zhang W, Sartori MA, Makhnevych T, Federowicz KE, Dong X, Liu L, Nim S, Dong A, Yang J, Li Y, et al.

(18) Molecular basis of USP7 inhibition by selective small-molecule inhibitorsNatureOctober 26, 2017Turnbull AP, Ioannidis S, Krajewski WW, Pinto-Fernandez A, Heride C, Martin ACL, Tonkin LM, Townsend EC, Buker SM, Lancia DR, et al.

(19) Isolation and Characterization of RNA Aptamers against a Proteasome-Associated Deubiquitylating Enzyme UCH37Chembiochem : a European journal of chemical biologyJanuary 17, 2017Lee JH, Lee MJ.

Be the First to Write a Review!

Ubiquitin Rhodamine 110 C-Terminal Derivative Rhodamine 110-conjugated Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (19)

Reviews

Ubiquitin Rhodamine 110 C-Terminal Derivative Rhodamine 110-conjugated Protein from R&D Systems, a Bio-Techne Brand

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map