Fig 1: Mechanistic studies of Jun13296.a Differential scanning fluorimetry assay of Jun13296 in stabilizing SARS-CoV-2 PLpro. Jun12682 was included as a positive control for comparison. Data from Jun12682 is the mean of two repeats, and data from Jun13296 is the mean ± standard deviation of three technical repeats. b Ki plot of Jun13296 in inhibiting SARS-CoV-2 PLpro hydrolysis of ISG15-AMC. c Ki plot of Jun13296 in inhibiting SARS-CoV-2 PLpro hydrolysis of Ub-AMC. d Counter screening of Jun13296 against host proteases USP2, USP7, USP8, USP14, USP15, USP30, UCH-L1, cathepsin B, cathepsin K, calpain-1, trypsin, and caspase 3. Data in (d) are presented as mean ± standard deviation of two technical repeats. Source data are provided as a source data file.