Fig 1: METTL3, METTL14, and WTAP level in A549 cells of all groups. C: control group without treatment; G: TGF-β inhibitor added group; P: PM2.5 added group; PG: PM2.5 and TGF-β inhibitor simultaneously added group. (a,b) PM2.5 induced up-regulation of METTL3 gene and protein expression in A549 was reversed after TGF-β inhibitor treatment. (c,d) PM2.5 induced up-regulation of METTL14 gene and protein expression in A549 was reversed after TGF-β inhibitor treatment. (e,f) WTAP gene and protein expression did not change significantly after PM2.5 exposure. *: p < 0.05; **: p < 0.01; ***: p < 0.001.
Fig 2: RNA m6A level in A549 cells after PM2.5 exposure. PM2.5 with a concentration of 100 μg/mL exposure induced a significant up-regulation of RNA m6A level in A549 cells. *: p < 0.05.
Fig 3: The RNA m6A levels of A549 cells of all groups. C: control group without treatment; G: TGF-β inhibitor added group; P: PM2.5 added group; PG: PM2.5 and TGF-β inhibitor simultaneously added group. PM2.5 induced up-regulation of RNA m6A level in A549 was reversed after TGF-β inhibitor treatment. *: p < 0.05; **: p < 0.01; ***: p < 0.001.
Supplier Page from Abcam for Recombinant Human METTL3 protein (GST tag N-Terminus)