Fig 1: The subcellular localization of human DEGS1 in SH-SY5Y cells(A–D)Representative confocal images of SH-SY5Y cells immunostained with antibodies against specific subcellular compartments including anti-KDEL for the ER (A), anti-Rab5 for the endosomes (B), anti-CD63 for vesicles (C), and anti-GM130 for the Golgi apparatus (D). The cells were co-stained with anti-DEGS1 antibodies (A′-D′) and merged with DAPI (A″-D″).(E) Pearson's coefficient of DEGS1 and the markers of subcellular compartments. Scale bars: 5 μm. The bar chart shows the mean ± SEM of at least three independent experiments.
Fig 2: DEGS1 drives the formation of ILVs in the giant unilamellar vesicles (GUVs) in vitro(A) Table summary of the percentages of GUVs that formed ILVs within 5 min with or without dhCer and recombinant DEGS1 protein.(B–E) Representative images of GUVs in (A).(F) Table summary of the percentages of GUVs that showed ILV formation within 5 min with and without DEGS1 protein in the presence of ethanol (EtOH) solvent or 0.2 μM fenretinide, a DEGS1 inhibitor.(G–J)Representative images of GUVs in (F). Scale bar: 5 μm.See also Figure S5.
Supplier Page from Abcam for Recombinant Human MLD protein